公开(公告)号：US20230212650A1

公开(公告)日：2023-07-06

申请号：US18181263

申请日：2023-03-09

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  David Michael Patterson

IPC: C12Q1/6806

CPC classification number: C12Q1/6806

Abstract: Provided herein are methods, compositions, and kits for determining a location of a target analyte in a biological sample that include the use of terminal deoxynucleotidyl transferase.

公开(公告)号：US20210324457A1

公开(公告)日：2021-10-21

申请号：US17271866

申请日：2019-08-27

Applicant: Eswar Prasad RAMACHANDRAN IYER ,  Michael SCHNALL-LEVIN ,  Augusto Manuel TENTORI ,  Tarje Sigurd MIKKELSEN ,  Benjamin HINDSON ,  Michael Ybarra LUCERO ,  Rajiv BHARADWAJ ,  Yifeng YIN ,  Zachary BENT ,  Jennifer CHEW ,  Meghan L. F. FREY ,  Linda KVASTAD ,  Joakim LUNDEBERG ,  Eva Gracia VILLACAMPA ,  10x Genomics, Inc.

Inventor： Eswar Prasad Ramachandran Iyer ,  Michael Schnall-Levin ,  Augusto Manuel Tentori ,  Tarjei Sigurd Mikkelsen ,  Benjamin Hindson ,  Michael Ybarra Lucero ,  Rajiv Bharadwaj ,  Yifeng Yin ,  Zachary Bent ,  Jennifer Chew ,  Meghan L. F. Frey ,  Linda Kvastad ,  Joakim Lundeberg ,  Eva Gracia Villacampa

IPC: C12Q1/6837 ,  C12Q1/6841

Abstract: This disclosure relates to methods for generating spatially barcoded arrays using concentration gradients.

公开(公告)号：US11970739B2

公开(公告)日：2024-04-30

申请号：US18348063

申请日：2023-07-06

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Joseph Francis Shuga ,  Patrick Roelli ,  Denise Cheung ,  Alexander Hermes ,  Yifeng Yin ,  Naishitha Anaparthy ,  Ryo Hatori ,  Marlon Stoeckius ,  Rapolas Spalinskas ,  Anna-Maria Katsori ,  Cedric Uytingco ,  Mesruh Turkekul ,  David Sukovich ,  Christina Galonska ,  Aleksandra Jurek ,  Octavian Marian Bloju

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6837

Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

公开(公告)号：US20240011090A1

公开(公告)日：2024-01-11

申请号：US18348063

申请日：2023-07-06

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Joseph Francis Shuga ,  Patrick Roelli ,  Denise Cheung ,  Alexander Hermes ,  Yifeng Yin ,  Naishitha Anaparthy ,  Ryo Hatori ,  Marlon Stoeckius ,  Rapolas Spalinskas ,  Anna-Maria Katsori ,  Cedric Uytingco ,  Mesruh Turkekul ,  David Sukovich ,  Christina Galonska ,  Aleksandra Jurek ,  Octavian Marian Bloju

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6837

Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

公开(公告)号：US11808769B2

公开(公告)日：2023-11-07

申请号：US18160084

申请日：2023-01-26

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  James Michael Chell ,  Marlon Stoeckius ,  Albert Dale Kim ,  Dulce Ovando Morales ,  Andrej Hartnett ,  Yifeng Yin ,  Jennifer Chew

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/64 ,  G01N33/58

CPC classification number: G01N33/6803 ,  C12N15/1065 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/6428 ,  G01N21/6456 ,  G01N33/582 ,  G01N2021/6439

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

公开(公告)号：US20230332212A1

公开(公告)日：2023-10-19

申请号：US18311644

申请日：2023-05-03

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Marlon Stoeckius

IPC: C12Q1/6837 ,  C12Q1/48 ,  G01N1/30

CPC classification number: C12Q1/6837 ,  C12Q1/48 ,  G01N1/30

Abstract: Provided herein are methods and kits for binding of an analyte capture sequence to a capture domain of a capture probe.

公开(公告)号：US11739381B2

公开(公告)日：2023-08-29

申请号：US17867223

申请日：2022-07-18

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Joseph Francis Shuga ,  Patrick Roelli ,  Denise Cheung ,  Alexander Hermes ,  Yifeng Yin ,  Naishitha Anaparthy ,  Ryo Hatori ,  Marlon Stoeckius ,  Rapolas Spalinskas ,  Anna-Maria Katsori ,  Cedric Uytingco ,  Mesruh Turkekul ,  David Sukovich ,  Christina Galonska ,  Aleksandra Jurek ,  Octavian Marian Bloju

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6837

Abstract: Provided herein are methods, compositions, and kits for preparing biological samples for multiplex spatial gene expression and proteomic analysis, such as determining a location of a nucleic acid analyte and a protein analyte in a biological sample.

公开(公告)号：US20230081381A1

公开(公告)日：2023-03-16

申请号：US17801078

申请日：2021-02-19

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew

IPC: C12Q1/6874

Abstract: Provided herein are methods of identifying the spatial location of a nucleic acid in a biological sample. In some embodiments, the methods employ a template switching oligonucleotide. In some embodiments, the methods extend the capture probe to create a complementary DNA (cDNA) molecule of a capture analyte and produce second strand in one reaction.

公开(公告)号：US11592447B2

公开(公告)日：2023-02-28

申请号：US17738808

申请日：2022-05-06

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  James Michael Chell ,  Marlon Stoeckius ,  Albert Kim ,  Dulce Ovando Morales ,  Andrej Hartnett ,  Yifeng Yin ,  Jennifer Chew

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/64 ,  G01N33/58

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

公开(公告)号：US11560592B2

公开(公告)日：2023-01-24

申请号：US17548047

申请日：2021-12-10

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Zachary Bent ,  Alvaro J Gonzalez Lozano

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6825

Abstract: Provided herein are methods for resetting an array to which a biological sample has been applied that include treating the array with a set of biological sample removal conditions.