公开(公告)号：US6046037A

公开(公告)日：2000-04-04

申请号：US434000

申请日：1995-05-04

申请人： Andrew C. Hiatt ,  Julian K.-C. Ma ,  Thomas Lehner ,  Keith E. Mostov

发明人： Andrew C. Hiatt ,  Julian K.-C. Ma ,  Thomas Lehner ,  Keith E. Mostov

IPC分类号： C07K14/705 ,  C07K16/00 ,  C12N15/82 ,  C12N15/00 ,  A01H4/00 ,  C12N15/29

CPC分类号： C12N15/8258 ,  C07K14/705 ,  C07K16/00

摘要： The immunoglobulins of the present invention are useful therapeutic immunoglobulins against mucosal pathogens such as S. mutans. The immunoglobulins contain a protection protein that protects the immunoglobulins in the mucosal environment.The invention also includes the greatly improved method of producing immunoglobulins in plants by producing the protection protein in the same cell as the other components of the immunoglobulins. The components of the immunoglobulin are assembled at a much improved efficiency. The method of the invention allows the assembly and high efficiency production of such complex molecules.The invention also contemplates the production of immunoglobulins containing protection proteins in a variety of cells, including plant cells, that can be selected for useful additional properties. The use of immunoglobulins containing protection proteins as therapeutic antibodies against mucosal and other pathogens is also contemplated.

摘要翻译： 本发明的免疫球蛋白是针对粘膜病原体如变形链球菌的有用的治疗性免疫球蛋白。 免疫球蛋白含有保护蛋白质，其保护粘膜环境中的免疫球蛋白。 本发明还包括通过在与免疫球蛋白的其它组分相同的细胞中产生保护性蛋白质来大大改进在植物中产生免疫球蛋白的方法。 以非常改进的效率组装免疫球蛋白的组分。 本发明的方法允许这种复合分子的组装和高效生产。 本发明还考虑在多种细胞（包括植物细胞）中产生含有保护性蛋白质的免疫球蛋白，其可以选择用于有用的附加性质。 还考虑使用含有保护性蛋白质的免疫球蛋白作为针对粘膜和其它病原体的治疗性抗体。

公开(公告)号：US20080115244A1

公开(公告)日：2008-05-15

申请号：US11605720

申请日：2006-11-28

申请人： Andrew C. Hiatt ,  Julian K.-C. Ma ,  Thomas Lehner

发明人： Andrew C. Hiatt ,  Julian K.-C. Ma ,  Thomas Lehner

IPC分类号： C12N15/82 ,  C07K16/18 ,  C12N9/14 ,  C12P21/04

CPC分类号： C07K16/1278 ,  C07K14/705 ,  C07K16/00 ,  C07K2317/13 ,  C07K2318/10 ,  C12N15/8242 ,  C12N15/8258

摘要： The immunoglobulins of the present invention are useful therapeutic immunoglobulins against mucosal pathogens such as S. mutans. The immunoglobulins contain a protection protein that protects the immunoglobulins in the mucosal environment.The invention also includes the greatly improved method of producing immunoglobulins in plants by producing the protection protein in the same cell as the other components of the immunoglobulins. The components of the immunoglobulin are assembled at a much improved efficiency. The method of the invention allows the assembly and high efficiency production of such complex molecules.The invention also contemplates the production of immunoglobulins containing protection proteins in a variety of cells, including plant cells, that can be selected for useful additional properties. The use of immunoglobulins containing protection proteins as therapeutic antibodies against mucosal and other pathogens is also contemplated.

摘要翻译： 本发明的免疫球蛋白是针对粘膜病原体如变形链球菌的有用的治疗性免疫球蛋白。 免疫球蛋白含有保护蛋白质，其保护粘膜环境中的免疫球蛋白。 本发明还包括通过在与免疫球蛋白的其它组分相同的细胞中产生保护性蛋白质来大大改进在植物中产生免疫球蛋白的方法。 以非常改进的效率组装免疫球蛋白的组分。 本发明的方法允许这种复合分子的组装和高效生产。 本发明还考虑在多种细胞（包括植物细胞）中产生含有保护性蛋白质的免疫球蛋白，其可以选择用于有用的附加性质。 还考虑使用含有保护性蛋白质的免疫球蛋白作为针对粘膜和其它病原体的治疗性抗体。

公开(公告)号：US06214987B1

公开(公告)日：2001-04-10

申请号：US08486885

申请日：1995-06-07

申请人： Andrew C. Hiatt ,  Floyd Rose

发明人： Andrew C. Hiatt ,  Floyd Rose

IPC分类号： C07H1904

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12P19/30 ,  Y02P20/55

摘要： A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3′-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3′-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5′ to 3′ phosphodiester linkage of the 5′-phosphate of the mononucleotide to the 3′-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3′-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3′-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.

公开(公告)号：US6136568A

公开(公告)日：2000-10-24

申请号：US929856

申请日：1997-09-15

申请人： Andrew C. Hiatt ,  Floyd D. Rose

发明人： Andrew C. Hiatt ,  Floyd D. Rose

IPC分类号： C12N15/10 ,  C12Q1/68 ,  C07H19/00 ,  C07H21/04 ,  C12P19/34

CPC分类号： C12N15/1027 ,  C12N15/10 ,  Y10S435/81

摘要： Disclosed is a method for synthesizing polynucleotide molecules such as genes or gene segments. A primer having 5' and 3' ends is incubated with a relatively shorter template having a 5' region non-complementary to the primer, a 3' region complementary to the 3' end of the primer, and a non-reactive 3' terminus to allow the 3' region of the template to anneal to the primer. The annealed product is reacted with at least one nucleotide in the presence of a template-dependent polynucleotide polymerase to produce a primer extended at its 3' end by at least one nucleotide complementary to the 5' region of the template. The extended primer is then dissociated from the template. The extended primer is further extended by repeating this cycle for sufficient cycles, wherein the templates and enzymes may differ from cycle to cycle, to obtain the object polynucleotide. Also disclosed are template libraries and kits containing said libraries for use in conjunction with the polynucleotide synthesis method.

摘要翻译： 公开了用于合成多基因分子例如基因或基因片段的方法。 将具有5'和3'末端的引物与相对短的模板孵育，其具有与引物不互补的5'区域，与引物3'末端互补的3'区域和非反应性3'末端 以允许模板的3'区域与引物退火。 退火的产物在模板依赖性多核苷酸聚合酶存在下与至少一个核苷酸反应，以产生在其3'末端延伸至少一个与模板的5'区域互补的核苷酸的引物。 然后将扩展的引物与模板分离。 通过重复该循环足够的循环来进一步延长延伸的引物，其中模板和酶可以不同于循环，以获得目的多核苷酸。 还公开了包含与多核苷酸合成方法结合使用的所述文库的模板文库和试剂盒。

公开(公告)号：US5990300A

公开(公告)日：1999-11-23

申请号：US300484

申请日：1994-09-02

申请人： Andrew C. Hiatt ,  Floyd Rose

发明人： Andrew C. Hiatt ,  Floyd Rose

IPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12P19/30 ,  C07H1/00

CPC分类号： C07H19/10 ,  C07H19/20 ,  C07H21/00 ,  C12P19/30 ,  Y02P20/55

摘要： A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.

摘要翻译： 通过制备含有游离和未修饰的3'-羟基的引发底物，在期望的核苷之间逐步产生磷酸二酯键的方法，导致合成具有预定核苷酸序列的多核苷酸; 将根据预定核苷酸序列的顺序选择的单核苷酸与起始底物的3'-羟基在含有催化量的能够催化5'-磷酸的5'至3'磷酸二酯键的酶的溶液中 的单核苷酸与起始底物的3'-羟基反应，其中单核苷酸含有受保护的3'-羟基，由此受保护的单核苷酸与起始底物共价连接，另外的加成受到3'-羟基保护基 。 还公开了将固定在固体支持物上的单核苷酸加入到游离多核苷酸链中的方法。