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    METHOD OF PRODUCING LACTIC ACID BY CONTINUOUS FERMENTATION
    22.
    发明申请
    METHOD OF PRODUCING LACTIC ACID BY CONTINUOUS FERMENTATION 审中-公开
    连续发酵生产酸乳酸的方法

    公开(公告)号：US20140017746A1

    公开(公告)日：2014-01-16

    申请号：US14027754

    申请日：2013-09-16

    申请人： Nanami Sasaki ,  Ken Morita ,  Takashi Mimitsuka ,  Hideki Sawai ,  Katsushige Yamada

    发明人： Nanami Sasaki ,  Ken Morita ,  Takashi Mimitsuka ,  Hideki Sawai ,  Katsushige Yamada

    IPC分类号： C12P7/56

    CPC分类号： C12P7/56

    摘要： A method of producing lactic acid by continuous fermentation includes filtering a culture of polyploidy prototrophic yeast having a capacity to produce lactic acid by introduction of a lactate dehydrogenase gene through a porous membrane having an average pore size of not less than 0.01 μm and less than 1 μm; and recovering the lactic acid from filtrate while the unfiltered liquid is retained in or returned to the culture and a fermentation feedstock is added to the culture.

    摘要翻译： 通过连续发酵生产乳酸的方法包括通过将平均孔径不小于0.01μm和小于1的多孔膜引入乳酸脱氢酶基因来过滤具有产生乳酸能力的多倍体原养酵母的培养物 妈妈 并从滤液中回收乳酸，同时将未过滤的液体保留在培养物中或返回到培养物中，向培养物中加入发酵原料。

    METHOD FOR PRODUCING LACTIC ACID AND METHOD FOR PRODUCING POLYLACTIC ACID
    23.
    发明申请
    METHOD FOR PRODUCING LACTIC ACID AND METHOD FOR PRODUCING POLYLACTIC ACID 审中-公开
    生产乳酸的方法和生产聚酸的方法

    公开(公告)号：US20110263811A1

    公开(公告)日：2011-10-27

    申请号：US13142062

    申请日：2009-12-25

    申请人： Kenji Sawai ,  Hideki Sawai ,  Takashi Mimitsuka ,  Ito Masateru ,  Katsushige Yamada ,  Kenji Kawamura ,  Shin-ichi Minegishi ,  Izumi Nakagawa ,  Tatsuya Nagano

    发明人： Kenji Sawai ,  Hideki Sawai ,  Takashi Mimitsuka ,  Ito Masateru ,  Katsushige Yamada ,  Kenji Kawamura ,  Shin-ichi Minegishi ,  Izumi Nakagawa ,  Tatsuya Nagano

    IPC分类号： C08G63/78 ,  C07D319/12 ,  C12P7/56

    摘要： Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 μm and less than 1 μm with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25° C. to 200° C. to recover lactic acid.

    摘要翻译： 乳酸通过以下方法获得：（A）连续发酵步骤，其中具有乳酸发酵能力的微生物的发酵培养基通过平均孔径不小于0.01μm的多孔膜过滤 超过1μm，跨膜压力差在0.1〜20kPa的范围内，并收集渗透物，同时将非渗透液体保留在或将非渗透液体返回到培养物中，并向培养物中加入发酵原料 ; （B）通过纳滤膜对步骤（A）中得到的渗透物进行过滤的工序; 和（C）在25℃至200℃下，在不低于1Pa且不大于大气压的压力下，将步骤（B）中得到的渗透物蒸馏的步骤，以回收乳酸。

    Method of stabilizing useful protein and useful protein compositions
    25.
    发明授权
    Method of stabilizing useful protein and useful protein compositions 有权
    稳定有用蛋白质和有用的蛋白质组成的方法

    公开(公告)号：US06391296B1

    公开(公告)日：2002-05-21

    申请号：US09269833

    申请日：1999-04-26

    申请人： Fumiyoshi Okano ,  Katsushige Yamada ,  Masatoshi Watanabe ,  Naomi Hara ,  Akira Yanai

    发明人： Fumiyoshi Okano ,  Katsushige Yamada ,  Masatoshi Watanabe ,  Naomi Hara ,  Akira Yanai

    IPC分类号： A61K4500

    CPC分类号： C07K14/555 ,  A61K38/00

    摘要： A method of stabilizing a useful protein by mixing a useful protein and an aqueous solution of a compound having the basic structure of arabic acid, and a stabilizied useful protein compositon containing a useful protein and a compound having the basic structure of arabic acid; gum arabic is preferred as a compound having the basic structure of arabic acid, and examples of a useful protein include cytokine and interferon; and a production method for canine interferon-&ggr; and stabilization thereof.

    摘要翻译： 通过混合有用蛋白质和具有阿拉伯酸碱性结构的化合物的水溶液和含有有用蛋白质的稳定化有用蛋白质组合物和具有阿拉伯酸碱性结构的化合物来稳定有用蛋白质的方法; 优选阿拉伯树胶作为具有阿拉伯酸碱性结构的化合物，有用的蛋白质的实例包括细胞因子和干扰素; 以及犬干扰素γ的制造方法及其稳定化。

    Polypeptide having D-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of D-lactic acid
    29.
    发明授权
    Polypeptide having D-lactate dehydrogenase activity, polynucleotide encoding the polypeptide, and process for production of D-lactic acid 有权
    具有D-乳酸脱氢酶活性的多肽，编码多肽的多核苷酸，以及D-乳酸的生产方法

    公开(公告)号：US08822195B2

    公开(公告)日：2014-09-02

    申请号：US13375882

    申请日：2010-06-02

    申请人： Kenji Sawai ,  Kazumi Suda ,  Hideki Sawai ,  Katsushige Yamada ,  Junya Yamagishi

    发明人： Kenji Sawai ,  Kazumi Suda ,  Hideki Sawai ,  Katsushige Yamada ,  Junya Yamagishi

    IPC分类号： C12N1/20 ,  C12N15/00 ,  C12N9/04 ,  C12P7/56

    CPC分类号： C12P7/56 ,  C12N9/0006

    摘要： Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.

    摘要翻译： 高生产率D-乳酸发酵使用通过向宿主细胞中引入编码根据下列（A）至（C）中任一项所述的多肽的多核苷酸而获得的转化体，使得多肽表达， D-乳酸脱氢酶活性高于常规多肽的活性：（A）具有SEQ ID NO：1或2所示氨基酸序列的多肽; （B）具有与SEQ ID NO：1或2所示相同氨基酸序列的多肽，不同之处在于一个或几个氨基酸被取代，缺失，插入和/或添加，该多肽具有D-乳酸脱氢酶活性; 和（C）具有与SEQ ID NO：1或2所示的氨基酸序列具有不小于80％的序列同一性的氨基酸序列的多肽，该多肽具有D-乳酸脱氢酶活性。