公开(公告)号：US09587253B2

公开(公告)日：2017-03-07

申请号：US12280197

申请日：2007-02-16

申请人： Hideki Sawai ,  Katsushige Yamada ,  Takashi Mimitsuka ,  Kenji Sawai ,  Tetsu Yonehara ,  Yohito Ito ,  Masahiro Henmi

发明人： Hideki Sawai ,  Katsushige Yamada ,  Takashi Mimitsuka ,  Kenji Sawai ,  Tetsu Yonehara ,  Yohito Ito ,  Masahiro Henmi

IPC分类号： C12P13/04 ,  C12P7/06 ,  C12M1/00 ,  C12M1/34 ,  C12P7/18 ,  C12P7/46 ,  C12P7/50 ,  C12P7/56 ,  C12P13/00 ,  C12P13/08 ,  C12P19/30

CPC分类号： C12P7/06 ,  C12M29/04 ,  C12M29/18 ,  C12M41/40 ,  C12M47/10 ,  C12P7/18 ,  C12P7/46 ,  C12P7/50 ,  C12P7/56 ,  C12P13/001 ,  C12P13/005 ,  C12P13/04 ,  C12P13/08 ,  C12P19/30 ,  Y02E50/17

摘要： The invention provides a method of producing a chemical product through continuous fermentation which includes filtering a culture of a microorganism or cultured cells with a separation membrane to recover a product from a filtrate and simultaneously retaining a nonfiltered fluid in, or refluxing it to, the culture, and adding fermentation materials to the culture, wherein a porous membrane having an average pore size of 0.01 μm or more to less than 1 μm is used as the separation membrane and the filtration is conducted with a transmembrane pressure difference in the range of 0.1 to 20 kPa. According to this method, the fermentation productivity of the chemical product can be largely elevated at high stability and a low cost.

摘要翻译： 本发明提供了一种通过连续发酵生产化学产品的方法，包括用分离膜过滤微生物培养物或培养细胞，从滤液中回收产物，同时将非过滤液体保留在培养基中或将其回流至培养物中 将发酵材料添加到培养物中，其中使用平均孔径为0.01μm以上且小于1μm的多孔膜作为分离膜，并且以跨膜压差为0.1〜 20 kPa。 根据该方法，化学产品的发酵生产率可以在高稳定性和低成本的情况下大大提高。

公开(公告)号：US20120070871A1

公开(公告)日：2012-03-22

申请号：US13375882

申请日：2010-06-02

申请人： Kenji Sawai ,  Kazumi Suda ,  Hideki Sawai ,  Katsushige Yamada ,  Junya Yamagishi

发明人： Kenji Sawai ,  Kazumi Suda ,  Hideki Sawai ,  Katsushige Yamada ,  Junya Yamagishi

IPC分类号： C12P7/56 ,  C12N1/19 ,  C12N15/63 ,  C12N9/04 ,  C12N15/53

CPC分类号： C12P7/56 ,  C12N9/0006

摘要： Highly productive D-lactic acid fermentation uses a transformant obtained by introducing into a host cell a polynucleotide encoding a polypeptide according to any one of the following (A) to (C) in such a manner that the polypeptide is expressed, which polypeptide has a D-lactate dehydrogenase activity higher than those of conventional polypeptides: (A) a polypeptide having the amino acid sequence shown in SEQ ID NO:1 or 2; (B) a polypeptide having the same amino acid sequence as shown in SEQ ID NO:1 or 2 except that one or several amino acids are substituted, deleted, inserted and/or added, which polypeptide has a D-lactate dehydrogenase activity; and (C) a polypeptide having an amino acid sequence which has a sequence identity of not less than 80% to the amino acid sequence shown in SEQ ID NO:1 or 2, which polypeptide has a D-lactate dehydrogenase activity.

摘要翻译： 高生产率D-乳酸发酵使用通过向宿主细胞中引入编码根据下列（A）至（C）中任一项所述的多肽的多核苷酸而获得的转化体，使得多肽表达， D-乳酸脱氢酶活性高于常规多肽的活性：（A）具有SEQ ID NO：1或2所示氨基酸序列的多肽; （B）具有与SEQ ID NO：1或2所示相同氨基酸序列的多肽，不同之处在于一个或几个氨基酸被取代，缺失，插入和/或添加，该多肽具有D-乳酸脱氢酶活性; 和（C）具有与SEQ ID NO：1或2所示的氨基酸序列具有不小于80％的序列同一性的氨基酸序列的多肽，该多肽具有D-乳酸脱氢酶活性。

公开(公告)号：US08071357B2

公开(公告)日：2011-12-06

申请号：US12083315

申请日：2006-09-04

申请人： Hideki Sawai ,  Kenji Sawai ,  Tomonori Sonoki ,  Satoko Hatahira

发明人： Hideki Sawai ,  Kenji Sawai ,  Tomonori Sonoki ,  Satoko Hatahira

IPC分类号： C12N1/00 ,  C12N1/11 ,  C12N1/16

CPC分类号： C12N9/0006 ,  C12P7/56

摘要： Yeast includes an introduced gene coding a Homo sapiens- or frog-derived L-lactate dehydrogenase. It is possible to produce lactic acid, which has a variety of applications, efficiently and more cost-effectively by using the yeast and the method of producing lactic acid by using the yeast.

摘要翻译： 酵母包括编码智人或青蛙衍生的L-乳酸脱氢酶的引入基因。 通过使用酵母和通过使用酵母生产乳酸的方法，可以生产具有多种应用的乳酸，有效且更经济有效。

公开(公告)号：US20100190222A1

公开(公告)日：2010-07-29

申请号：US12666690

申请日：2008-06-18

申请人： Masateru Ito ,  Shin-ichi Minegishi ,  Eri Shimizu ,  Kenji Sawai ,  Yohito Ito ,  Hideki Sawai ,  Katsushige Yamada

发明人： Masateru Ito ,  Shin-ichi Minegishi ,  Eri Shimizu ,  Kenji Sawai ,  Yohito Ito ,  Hideki Sawai ,  Katsushige Yamada

IPC分类号： C12P7/56

CPC分类号： C12P7/56 ,  B01D61/027 ,  B01D71/56 ,  B01D2311/06 ,  B01D2311/18 ,  B01D2311/2669

摘要： Disclosed is a lactic acid production method by separating lactic acid produced in a culture solution by means of the fermentation culture of a microorganism. Specifically disclosed is a lactic acid production method, which comprises: a step (A) of filtering the culture solution through a nano-filtration membrane; and a step (B) of distilling a lactic-acid-containing solution produced in the step (A) under a pressure ranging from 1 Pa to the atmospheric pressure (inclusive) at a temperature ranging from 25 to 200° C. (inclusive) to collect lactic acid. The method can effectively remove an inorganic salt dissolved in a fermentation culture solution or contained in the fermentation culture solution in the form of a poorly soluble solid material by a simple manipulation, enables to prevent the racemization or oligomerization of lactic acid during the process of producing lactic acid, and therefore can produce lactic acid in a high yield.

摘要翻译： 公开了通过微生物的发酵培养分离培养液中产生的乳酸的乳酸生产方法。 具体公开的是乳酸的制造方法，其包括：通过纳滤膜过滤培养液的工序（A） 和（B）在步骤（A）中制备的含乳酸的溶液在25Pa至200℃的温度范围内，在1Pa至大气压（含）范围内， 收集乳酸。 该方法可以通过简单的操作有效地除去溶解在发酵培养液中的无机盐或发酵培养液中含有的难溶性固体物质的形式的无机盐，能够防止生产过程中乳酸的外消旋化或低聚反应 乳酸，因此可以高产率生产乳酸。

公开(公告)号：US20060260190A1

公开(公告)日：2006-11-23

申请号：US10568500

申请日：2005-04-14

申请人： Norihisa Miyoshi ,  Takashi Imaizumi ,  Kenji Sawai ,  Morio Iriyama ,  Shutaro Takakura

发明人： Norihisa Miyoshi ,  Takashi Imaizumi ,  Kenji Sawai ,  Morio Iriyama ,  Shutaro Takakura

IPC分类号： C02F3/28

CPC分类号： F02C6/18 ,  C10J3/482 ,  C10J2300/0903 ,  C10J2300/0909 ,  C10J2300/0923 ,  C10J2300/0946 ,  C10J2300/165 ,  C10J2300/1884 ,  C10J2300/1892 ,  F02C3/28 ,  F02D19/04 ,  F23G5/0276 ,  F23G5/04 ,  F23G5/30 ,  F23G5/46 ,  F23G7/001 ,  F23G2203/502 ,  F23G2203/503 ,  F23G2206/10 ,  F23G2206/202 ,  F23G2206/203 ,  F23G2209/12 ,  F23J15/06 ,  F23J2219/70 ,  F23L2900/00001 ,  Y02E20/12 ,  Y02E20/14 ,  Y02E20/363 ,  Y02E50/11 ,  Y02E50/12 ,  Y02P20/129 ,  Y02P20/131 ,  Y02T10/34 ,  Y02W10/30

摘要： An organic matter such as sewage sludge (101) is treated by gasifying the organic matter into a combustible gas and using the combustible gas as a fuel for a gas engine or a gas turbine to recover power (energy). In a method of treating the organic matter, an organic matter as a raw material is dried in a drying process (51), the dried raw material is gasified to produce a generated gas (123), and the sensible heat from the generated gas and/or a combustion gas produced by gasification is recovered by using a heating medium in a recovery process. Then, the heating medium heated in the recovery process is introduced into the drying process as a heating medium gas for drying.

摘要翻译： 通过将有机物质气化成可燃性气体，利用可燃性气体作为燃气发动机或燃气轮机的燃料来回收功率（能量）来处理污水污泥（101）等有机物质。 在处理有机物的方法中，作为原料的有机物在干燥工序（51）中进行干燥，将干燥后的原料气化，生成气体（123），产生气体的显热和 /或通过在回收过程中使用加热介质回收通过气化产生的燃烧气体。 然后，将在回收工序中加热的加热介质作为加热介质气体引入干燥过程中进行干燥。

公开(公告)号：US10683254B2

公开(公告)日：2020-06-16

申请号：US13142062

申请日：2009-12-25

申请人： Kenji Sawai ,  Hideki Sawai ,  Takashi Mimitsuka ,  Ito Masateru ,  Katsushige Yamada ,  Kenji Kawamura ,  Shin-ichi Minegishi ,  Izumi Nakagawa ,  Tatsuya Nagano

发明人： Kenji Sawai ,  Hideki Sawai ,  Takashi Mimitsuka ,  Ito Masateru ,  Katsushige Yamada ,  Kenji Kawamura ,  Shin-ichi Minegishi ,  Izumi Nakagawa ,  Tatsuya Nagano

IPC分类号： C07C59/08 ,  B01D71/56 ,  C08G63/06 ,  C12P7/56 ,  C07C51/42 ,  B01D61/02

摘要： Lactic acid is obtained by a method including (A) a step of continuous fermentation wherein a fermentation culture medium of a microorganism having an ability of lactic acid fermentation is filtered through a porous membrane having an average pore size of not less than 0.01 μm and less than 1 μm with a transmembrane pressure difference within the range of 0.1 to 20 kPa, and the permeate is collected, while retaining the non-permeated liquid in or returning the non-permeated liquid to the culture, and adding a fermentation feedstock to the culture; (B) a step of filtering the permeate obtained in Step (A) through a nanofiltration membrane; and (C) a step of distilling the permeate obtained in Step (B) under a pressure of not less than 1 Pa and not more than atmospheric pressure, at 25° C. to 200° C. to recover lactic acid.

公开(公告)号：US09080190B2

公开(公告)日：2015-07-14

申请号：US13992081

申请日：2011-12-08

申请人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

发明人： Nanami Sasaki ,  Takashi Mimitsuka ,  Hideki Sawai ,  Kenji Sawai

IPC分类号： C12P13/00

CPC分类号： C12P13/001 ,  C12Y401/01018

摘要： A method produces cadaverine more efficiently and at a higher yield than production methods by the conventional fermentation methods. The method includes culturing coryneform bacterium/bacteria having an ability to produce cadaverine and having a resistance to 2,2′-thiobis(ethylamine). Preferably, the coryneform bacterium/bacteria has/have lysine decarboxylase activity and, preferably, the coryneform bacterium/bacteria has/have homoserine auxotrophy and/or a resistance to S-(2-aminoethyl)-L-cysteine.

摘要翻译： 一种方法通过常规发酵方法比生产方法更有效地产生尸胺，并且产量高。 该方法包括培养具有产生尸胺的能力并具有对2,2'-硫代双（乙胺）的抗性的棒状细菌/细菌。 优选地，棒状细菌/细菌具有/具有赖氨酸脱羧酶活性，优选棒状细菌/细菌具有高丝氨酸营养缺陷型和/或对S-（2-氨基乙基）-L-半胱氨酸的抗性。

公开(公告)号：US08859260B2

公开(公告)日：2014-10-14

申请号：US12746642

申请日：2008-12-05

申请人： Kenji Sawai ,  Hideki Sawai

发明人： Kenji Sawai ,  Hideki Sawai

IPC分类号： C12N1/19 ,  C12N9/04 ,  C12P7/56 ,  C12N15/81

CPC分类号： C12P7/56 ,  C12N9/0006 ,  C12N15/81

摘要： A gene expressing cassette codes lactate dehydrogenase that is needed for prevention of deterioration in lactic acid yield and lactic acid production rate in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability, which achieves high optical purity, high lactic acid yield and high lactic acid production rate simultaneously, a yeast strain having the cassette and a method of producing lactic acid by culturing the yeast strain. The lactate dehydrogenase-expressing cassette is a lactate dehydrogenase-expressing cassette, comprising a gene coding lactate dehydrogenase connected to a site downstream of a promoter, the promoter being a promoter of a gene showing a gene expression amount larger by 5 times or more than the average relative expression amount of all genes after 50 hours from start of culture in continuous culture with simultaneous filtration of a yeast strain having a lactic acid-producing ability.

摘要翻译： 表达盒的基因编码乳酸脱氢酶，其在连续培养中同时过滤具有乳酸生产能力的酵母菌株（其获得高光学纯度）的高乳酸所需的乳酸产率和乳酸生产率的降低所需的乳酸脱氢酶 产量和高乳酸生产率，具有盒的酵母菌株和通过培养酵母菌株产生乳酸的方法。 表达乳酸脱氢酶的盒是表达乳酸脱氢酶的盒，其包含编码与启动子下游位点连接的乳酸脱氢酶的基因，该启动子是基因表达量大于5倍以上的基因的启动子 同时过滤具有乳酸生产能力的酵母菌株的连续培养开始培养50小时后的所有基因的平均相对表达量。

公开(公告)号：US20130071888A1

公开(公告)日：2013-03-21

申请号：US13640793

申请日：2011-04-11

申请人： Kenji Sawai ,  Shiomi Watanabe ,  Takashi Mimitsuka ,  Hideki Sawai

发明人： Kenji Sawai ,  Shiomi Watanabe ,  Takashi Mimitsuka ,  Hideki Sawai

IPC分类号： C12N15/52

CPC分类号： C12N15/52 ,  C12N9/88 ,  C12P13/001 ,  C12Y401/01018

摘要： A method of producing 1,5-pentanediamine includes culturing coryneform bacterium having a gene encoding lysine decarboxylase in its chromosome, which coryneform bacterium maintains lysine decarboxylase activity of not less than 50 mU/mg protein during culturing and the gene encoding lysine decarboxylase is linked downstream of a promoter that functions during the logarithmic growth phase.

摘要翻译： 一种生产1,5-戊二胺的方法包括在其染色体中培养具有编码赖氨酸脱羧酶基因的棒状细菌，棒状细菌在培养期间保持不低于50mU / mg蛋白质的赖氨酸脱羧酶活性，编码赖氨酸脱羧酶的基因连接在下游 在对数生长期间起作用的启动子。

公开(公告)号：US08334411B2

公开(公告)日：2012-12-18

申请号：US12920891

申请日：2009-03-11

申请人： Masateru Ito ,  Izumi Nakagawa ,  Koya Kato ,  Takashi Mimitsuka ,  Kenji Sawai ,  Shin-ichi Minegishi ,  Hideki Sawai ,  Katsushige Yamada

发明人： Masateru Ito ,  Izumi Nakagawa ,  Koya Kato ,  Takashi Mimitsuka ,  Kenji Sawai ,  Shin-ichi Minegishi ,  Hideki Sawai ,  Katsushige Yamada

IPC分类号： C07C209/84 ,  C07C209/86 ,  C07C231/02

CPC分类号： C07C209/84 ,  B01D61/025 ,  B01D61/027 ,  B01D61/58 ,  B01D71/60 ,  B01D2317/022 ,  C08G69/26 ,  C08G69/28 ,  C07C211/09 ,  C07C211/12

摘要： A method for producing a diamine includes purifying a diamine from an aqueous solution containing a diamine salt by adding an alkaline substance to the aqueous solution and then filtering the resulting solution by allowing the solution to pass through a nanofiltration membrane to remove the salt, thereby obtaining an aqueous diamine solution.

摘要翻译： 二胺的制造方法包括：通过向水溶液中添加碱性物质，从含有二胺盐的水溶液中纯化二胺，然后通过使溶液通过纳滤膜除去盐而过滤所得溶液，从而得到 二胺水溶液。