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    Multi-Modal Fluorescence Imaging Flow Cytometry System
    21.
    发明申请
    Multi-Modal Fluorescence Imaging Flow Cytometry System 有权

    公开(公告)号：US20220268687A1

    公开(公告)日：2022-08-25

    申请号：US17745249

    申请日：2022-05-16

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64 ,  G02B21/06

    Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

    Multi-modal fluorescence imaging flow cytometry system
    22.
    发明授权
    Multi-modal fluorescence imaging flow cytometry system 有权

    公开(公告)号：US11366052B2

    公开(公告)日：2022-06-21

    申请号：US17153527

    申请日：2021-01-20

    Applicant: BECTON, DICKINSON AND COMPANY

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N21/64 ,  G01N15/14 ,  G02B21/06 ,  G01N15/10

    Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.

    Parameters for use in particle discrimination
    24.
    发明授权
    Parameters for use in particle discrimination 有权

    公开(公告)号：US11268890B2

    公开(公告)日：2022-03-08

    申请号：US16817331

    申请日：2020-03-12

    Applicant: Becton, Dickinson and Company

    Inventor： Matthew Bahr ,  Eric D. Diebold ,  Jonathan Lin ,  Keegan Owsley

    IPC: G01N15/02 ,  G01N15/14

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

    METHODS FOR MODULATING AN INTENSITY PROFILE OF A LASER BEAM AND SYSTEMS FOR SAME
    25.
    发明申请
    METHODS FOR MODULATING AN INTENSITY PROFILE OF A LASER BEAM AND SYSTEMS FOR SAME 有权

    公开(公告)号：US20210364413A1

    公开(公告)日：2021-11-25

    申请号：US17313457

    申请日：2021-05-06

    Applicant: Becton, Dickinson and Company

    Inventor： Matthew Bahr ,  Keegan Owsley ,  Jizuo Zou

    IPC: G01N15/14

    Abstract: Aspects of the present disclosure include methods for modulating an intensity profile of a laser beam. Methods according to certain embodiments include irradiating an acousto-optic device with a laser to generate an output laser beam having a plurality of angularly deflected laser beams, capturing an image of the output laser beam, determining an intensity profile of the output laser beam along a horizontal axis from the captured image and adjusting one or more parameters of a waveform inputted into the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile. Systems having a laser, an acousto-optic device, an imaging sensor and a waveform generator as well as non-transitory computer readable storage medium with instructions for practicing the subject methods are also described.

    Cell Sorting Using A High Throughput Fluorescence Flow Cytometer
    26.
    发明申请
    Cell Sorting Using A High Throughput Fluorescence Flow Cytometer 有权

    公开(公告)号：US20210356381A1

    公开(公告)日：2021-11-18

    申请号：US17386192

    申请日：2021-07-27

    Applicant: Becton, Dickinson and Company

    Inventor： Eric D. Diebold ,  Keegan Owsley ,  Jonathan Lin

    IPC: G01N15/14 ,  G01N21/64

    Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data.. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds, In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.

    Phase-Correction Of Radiofrequency-Multiplexed Signals
    29.
    发明申请
    Phase-Correction Of Radiofrequency-Multiplexed Signals 有权

    公开(公告)号：US20250035535A1

    公开(公告)日：2025-01-30

    申请号：US18817809

    申请日：2024-08-28

    Applicant: Becton, Dickinson and Company

    Inventor： Jonathan Lin ,  Keegan Owsley ,  Matthew Bahr

    IPC: G01N15/14 ,  G01N15/1404 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/149

    Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.

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