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公开(公告)号:US20220268687A1
公开(公告)日:2022-08-25
申请号:US17745249
申请日:2022-05-16
Applicant: BECTON, DICKINSON AND COMPANY
Inventor: Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号:US11366052B2
公开(公告)日:2022-06-21
申请号:US17153527
申请日:2021-01-20
Applicant: BECTON, DICKINSON AND COMPANY
Inventor: Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号:US20220101955A1
公开(公告)日:2022-03-31
申请号:US17545325
申请日:2021-12-08
Applicant: Becton, Dickinson and Company
Inventor: Jonathan Lin , Joseph Trotter , Keegan Owsley
Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.
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公开(公告)号:US11268890B2
公开(公告)日:2022-03-08
申请号:US16817331
申请日:2020-03-12
Applicant: Becton, Dickinson and Company
Inventor: Matthew Bahr , Eric D. Diebold , Jonathan Lin , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号:US20210364413A1
公开(公告)日:2021-11-25
申请号:US17313457
申请日:2021-05-06
Applicant: Becton, Dickinson and Company
Inventor: Matthew Bahr , Keegan Owsley , Jizuo Zou
IPC: G01N15/14
Abstract: Aspects of the present disclosure include methods for modulating an intensity profile of a laser beam. Methods according to certain embodiments include irradiating an acousto-optic device with a laser to generate an output laser beam having a plurality of angularly deflected laser beams, capturing an image of the output laser beam, determining an intensity profile of the output laser beam along a horizontal axis from the captured image and adjusting one or more parameters of a waveform inputted into the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile. Systems having a laser, an acousto-optic device, an imaging sensor and a waveform generator as well as non-transitory computer readable storage medium with instructions for practicing the subject methods are also described.
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公开(公告)号:US20210356381A1
公开(公告)日:2021-11-18
申请号:US17386192
申请日:2021-07-27
Applicant: Becton, Dickinson and Company
Inventor: Eric D. Diebold , Keegan Owsley , Jonathan Lin
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data.. In other words, while the fluorescence data can contain image data that would allow generating a pixel-by-pixel fluorescence intensity map, the method arrives at the sorting decision without generating such a map. In some cases, the sorting decision can be made with a latency less than about 100 microseconds, In some embodiments, the above method of sorting cells can have a sub-cellular resolution, e.g., the sorting decision can be based on characteristics of a component of the cell. In some embodiments in which more than two frequency-shifted optical frequencies are employed, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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公开(公告)号:US10006852B2
公开(公告)日:2018-06-26
申请号:US15698506
申请日:2017-09-07
Applicant: Becton, Dickinson and Company
Inventor: Eric D. Diebold , Keegan Owsley , Matthew Bahr
CPC classification number: G01N15/1434 , G01N15/1459 , G01N2015/1006 , G01N2015/145 , G02F1/33 , G02F2203/18 , G02F2203/24 , H01S3/0071
Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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28.
公开(公告)号:US12235201B2
公开(公告)日:2025-02-25
申请号:US17403105
申请日:2021-08-16
Applicant: Becton, Dickinson and Company
Inventor: Peter Mage , Lingjie Wei , Peter Johnson , David A. Roberts , Keegan Owsley
IPC: G01N15/10 , G01N15/14 , G01N15/1429 , G01N21/64 , G01N15/149
Abstract: Aspects of the present disclosure include methods for determining baseline noise of a photodetector (e.g., in a light detection system of a particle analyzer). Methods according to certain embodiments include irradiating a sample having particles in a flow stream, detecting light with the photodetector from the irradiated flow stream, generating data signals from the detected light and calculating a moving average mean squared error of the generated data signals to determine the baseline of the photodetector. Systems (e.g., particle analyzers) having a light source and a light detection system that includes a photodetector for practicing the subject methods are also described. Integrated circuits and non-transitory computer readable storage medium are also provided.
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公开(公告)号:US20250035535A1
公开(公告)日:2025-01-30
申请号:US18817809
申请日:2024-08-28
Applicant: Becton, Dickinson and Company
Inventor: Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14 , G01N15/1404 , G01N15/1429 , G01N15/1434 , G01N15/149
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号:US20240344964A1
公开(公告)日:2024-10-17
申请号:US18641039
申请日:2024-04-19
Applicant: Becton, Dickinson and Company
Inventor: Joseph T. Trotter , Keegan Owsley , Jonathan Lin
IPC: G01N15/14 , B07C5/342 , B07C5/36 , G01N15/1434 , G01N15/149 , G06V10/70 , G06V20/69 , G16C20/20
CPC classification number: G01N15/1459 , B07C5/3425 , B07C5/361 , G01N15/1425 , G01N15/1434 , G06V10/87 , G06V20/695 , G06V20/698 , G16C20/20 , G01N15/149
Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.
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