公开(公告)号：US10481158B2

公开(公告)日：2019-11-19

申请号：US15170919

申请日：2016-06-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James R. Heath ,  Songming Peng

IPC: G01N33/53 ,  C12Q1/68 ,  C07H21/04 ,  G01N33/569 ,  C12Q1/6804 ,  G01N15/14 ,  G01N33/574 ,  G01N33/68 ,  G01N15/00

Abstract: Compositions and methods for isolating patient-derived antigen-specific T cells include an antigen complex having a polynucleotide barcoded nanoparticle sorting agent complexed with a peptide-loaded streptavidin major histocompatability complex (MHC) tetramer, the barcoding technology allowing for high fidelity screening of a library of the antigen complexes to readily isolate and identify antigen-specific T cells.

公开(公告)号：US20180319844A1

公开(公告)日：2018-11-08

申请号：US16031840

申请日：2018-07-10

Applicant: California Institute of Technology

Inventor： Ryan K. Henning ,  Ashwin N. Ram ,  Samir Das ,  Arundhati Nag ,  James R. Heath

IPC: C07K7/64 ,  C07K7/06 ,  C07K14/82 ,  G01N33/574

CPC classification number: C07K7/64 ,  A61K38/00 ,  C07K7/06 ,  C07K14/82 ,  G01N33/5748 ,  G01N2333/82 ,  G01N2333/914 ,  G01N2500/04

Abstract: Cyclic peptides represented by (Formula 1) selectively bind the oncoprotein K-Ras G12D in vitro and in cellulo, where Z1 and Z2 are each L-propargylglycine (Pm), azidoornithine (OrnN3), or L-azidolysine (Az4), and V1-V2-V3-V4-V5 is an amino acid variable region having a sequence selected from the group consisting of SEQ ID NOs: 1-20.

公开(公告)号：US10017540B2

公开(公告)日：2018-07-10

申请号：US15068528

申请日：2016-03-11

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Ryan K. Henning ,  Ashwin N. Ram ,  Samir Das ,  Arundhati Nag ,  James R. Heath

IPC: A61K38/12 ,  A61P35/00 ,  C07K7/64 ,  G01N33/574 ,  C07K14/82 ,  C07K7/06 ,  A61K38/00

CPC classification number: C07K7/64 ,  A61K38/00 ,  C07K7/06 ,  C07K14/82 ,  G01N33/5748 ,  G01N2333/82 ,  G01N2333/914 ,  G01N2500/04

Abstract: Cyclic peptides represented by (Formula 1) selectively bind the oncoprotein K-Ras G12D in vitro and in cellulo, where Z1 and Z2 are each L-propargylglycine (Pra), azidoornithine (OrnN3), or L-azidolysine (Az4), and V1-V2-V3-V4-V5 is an amino acid variable region having a sequence selected from the group consisting of SEQ ID NOs: 1-20.

公开(公告)号：US20170218021A1

公开(公告)日：2017-08-03

申请号：US15420596

申请日：2017-01-31

Applicant: California Institute of Technology ,  Indi Molecular, Inc.

Inventor： Blake Farrow ,  James R. Heath ,  Heather Dawn Agnew

IPC: C07K7/08 ,  C07K7/06

CPC classification number: A61K38/005 ,  A61K38/00 ,  A61K38/06 ,  A61K38/08 ,  A61K38/12 ,  A61K47/60 ,  C07B59/008 ,  C07B2200/05 ,  C07K5/0808 ,  C07K7/06 ,  C07K7/08 ,  C07K7/52 ,  C07K2319/01 ,  C07K2319/70 ,  G01N33/531 ,  G01N2333/33 ,  G16B5/00 ,  Y02A50/469

Abstract: The present application provides stable peptide-based Botulinum neurotoxin (BoNT) serotype A capture agents and methods of use as detection and diagnosis agents and in the treatment of diseases and disorders. The application further provides methods of manufacturing BoNT serotype A capture agents using iterative on-bead in situ click chemistry.

公开(公告)号：US20170003288A1

公开(公告)日：2017-01-05

申请号：US15170919

申请日：2016-06-01

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： James R. Heath ,  Songming Peng

IPC: G01N33/569 ,  G01N15/14 ,  G01N33/574 ,  C12Q1/68 ,  G01N33/68

CPC classification number: G01N33/56972 ,  C07H21/04 ,  C12Q1/6804 ,  G01N15/14 ,  G01N33/574 ,  G01N33/6878 ,  G01N2015/008 ,  G01N2015/149 ,  G01N2333/70539 ,  G01N2458/10 ,  C12Q2563/131 ,  C12Q2563/143 ,  C12Q2563/155 ,  C12Q2565/514

Abstract: Compositions and methods for isolating patient-derived antigen-specific T cells include an antigen complex having a polynucleotide barcoded nanoparticle sorting agent complexed with a peptide-loaded streptavidin major histocompatability complex (MHC) tetramer, the barcoding technology allowing for high fidelity screening of a library of the antigen complexes to readily isolate and identify antigen-specific T cells.

Abstract translation: 用于分离患者衍生的抗原特异性T细胞的组合物和方法包括具有与加载肽的链霉抗生物素蛋白主要组织相容性复合物（MHC）四聚体复合的多核苷酸条形码纳米颗粒分选试剂的抗原复合物，该条形码技术允许文库的高保真度筛选 的抗原复合物以容易地分离和鉴定抗原特异性T细胞。

公开(公告)号：US20160238594A1

公开(公告)日：2016-08-18

申请号：US15045241

申请日：2016-02-16

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Min Xue ,  Wei Wei ,  James R. Heath

IPC: G01N33/53

CPC classification number: G01N33/5308 ,  G01N33/573

Abstract: Methods and compositions for quantifying the cellular concentration of cyclic adenosine monophosphate (cAMP), cyclic guanine monophosphate (cGMP), glutathione, glucose, and glutamine in a single cell include the use of conjugates and analogs in immunofluorescent assays for on-chip quantification. These metabolite immunofluorescent assays may be incorporated with proteomic assays for simultaneous single cell analysis of metabolites and proteins.

Abstract translation: 用于量化单个细胞中环腺苷一磷酸（cAMP），环鸟嘌呤一磷酸（cGMP），谷胱甘肽，葡萄糖和谷氨酰胺的细胞浓度的方法和组合物包括在免疫荧光测定中使用缀合物和类似物进行片上定量。 这些代谢物免疫荧光测定可以与代谢物和蛋白质的同时单细胞分析的蛋白质组测定结合。

公开(公告)号：US20160011189A1

公开(公告)日：2016-01-14

申请号：US14694340

申请日：2015-04-23

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Rong Fan ,  Habib Ahmad ,  James R. Heath

IPC: G01N33/543 ,  B01J19/00

CPC classification number: G01N33/54393 ,  B01J19/0046 ,  B01J2219/00547 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00621 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5025 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2300/0636 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0487 ,  B01L2400/084 ,  G01N33/54366 ,  G01N33/582 ,  G01N2458/10

Abstract: Arrays and substrates comprising a material, in particular capture agents and/or detectable targets, attached to the substrates along substantially parallel lines forming a barcoded pattern and related methods and systems.

公开(公告)号：US20150344523A1

公开(公告)日：2015-12-03

申请号：US14704865

申请日：2015-05-05

Applicant: California Institute of Technology

Inventor： Kaycie Deyle ,  Blake Farrow ,  James R. Heath

IPC: C07K7/06 ,  G01N33/573

CPC classification number: C07K7/06 ,  A61K38/00 ,  C07K7/00 ,  C07K14/001 ,  C12N9/1205 ,  G01N33/573 ,  G01N33/574 ,  G01N2333/912

Abstract: The present application provides stable peptide-based Akt capture agents and methods of use as detection and diagnosis agents and in the treatment of diseases and disorders. The application further provides methods of manufacturing Akt capture agents using iterative on-bead in situ click chemistry.

Abstract translation: 本申请提供了稳定的基于肽的Akt捕获剂以及用作检测和诊断试剂以及用于治疗疾病和病症的方法。 该应用进一步提供了使用迭代的珠粒原位点击化学制造Akt捕获剂的方法。

公开(公告)号：US20150099658A1

公开(公告)日：2015-04-09

申请号：US14484610

申请日：2014-09-12

Applicant: California Institute of Technology

Inventor： Jessica A. Pfeilsticker ,  Aiko Umeda ,  James R. Heath

IPC: G01N33/68 ,  C07K9/00

Abstract: The present application provides stable peptide-based anti-gp41 antibody capture agents and methods of use as detection and diagnosis agents. The application further provides methods of manufacturing anti-gp41 antibody capture agents using iterative on-bead in situ click chemistry.

Abstract translation: 本申请提供稳定的基于肽的抗gp41抗体捕获剂和用作检测和诊断试剂的方法。 该应用进一步提供使用迭代在珠粒原位点击化学制造抗gp41抗体捕获剂的方法。

公开(公告)号：US20150078999A1

公开(公告)日：2015-03-19

申请号：US14310201

申请日：2014-06-20

Applicant: California Institute of Technology

Inventor： James R. Heath ,  Rosemary Dyane Rohde ,  Arundhati Nag ,  Samir Das ,  Aiko Umeda

IPC: C07K7/64 ,  C07K14/00 ,  A61K51/08 ,  G01N33/68 ,  G01N33/573

CPC classification number: C07K7/64 ,  A61K49/0032 ,  A61K49/0041 ,  A61K49/0043 ,  A61K51/08 ,  A61K51/088 ,  C07K1/047 ,  C07K5/06 ,  C07K5/08 ,  C07K5/10 ,  C07K7/56 ,  C07K14/001 ,  G01N33/573 ,  G01N33/6842 ,  G01N2333/912

Abstract: Peptides having activity as protein binding agents are disclosed. The peptides have the following structure (I): including stereoisomers, pharmaceutically acceptable salts and prodrugs thereof, wherein R, R1, L1, L2, G, M, Y1 Y2 and SEQ are as defined herein. Methods associated with preparation and use of such peptides, as well as pharmaceutical compositions comprising such peptides, are also disclosed.

Abstract translation: 公开了具有作为蛋白质结合剂活性的肽。 肽具有以下结构（I）：包括其立体异构体，药学上可接受的盐和前药，其中R，R 1，L 1，L 2，G，M，Y 1 Y 2和SEQ如本文所定义。 还公开了与这种肽的制备和使用相关的方法，以及包含这些肽的药物组合物。