公开(公告)号：US10816554B2

公开(公告)日：2020-10-27

申请号：US15301930

申请日：2015-04-03

Applicant: The Board of Trustees of the Leland Stanford Junior University ,  CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Michael Edward Birnbaum ,  Juan Luis Mendoza ,  Michael Thomas Bethune ,  David Baltimore ,  Kenan Christopher Garcia

IPC: G01N33/68 ,  C07K14/00

Abstract: Compositions and methods are provided for the identification of peptide sequences that are ligands for a T cell receptor (TCR) of interest, in a given MHC context.

公开(公告)号：US10739351B2

公开(公告)日：2020-08-11

申请号：US15729253

申请日：2017-10-10

Applicant: California Institute of Technology

Inventor： Devdoot Majumdar ,  Anirudh Mathukumilli ,  David Baltimore

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6876

Abstract: Provided herein are method and kits for elucidation of immunological repertoires (comprising functional pairs of immune cell receptors and antigens). In some embodiments, an immune cell receptor and an antigen that it binds to are isolated, and sequence information is obtained about the immune cell receptor and antigen. In some embodiments, an antigen expressed on a first cell and an immune cell receptor expressed on a second cell can form a synapse, mRNAs can be isolated from the resulting doublet of cells, and sequences of the functional immune cell receptor and its antigen can be obtained from the mRNAs.

公开(公告)号：US10696984B2

公开(公告)日：2020-06-30

申请号：US16293151

申请日：2019-03-05

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Pin Wang ,  Lili Yang ,  David Baltimore

IPC: C12N5/00 ,  C12N15/00 ,  A01N63/00 ,  A01N65/00 ,  C12N15/86 ,  A61K38/17 ,  A61K31/70 ,  A61K38/19 ,  A61K38/20 ,  C07K14/005 ,  A61K35/76 ,  A61K39/21 ,  C12N7/00 ,  A61K39/00

Abstract: Methods and compositions are provided for delivery of a polynucleotide encoding a gene of interest, typically an antigen, to a dendritic cell (DC). The virus envelope comprises a DC-SIGN specific targeting molecule. The methods and related compositions can be used to treat patients suffering from a wide range of conditions, including infection, such as HIV/AIDS, and various types of cancers.

公开(公告)号：US20190316153A1

公开(公告)日：2019-10-17

申请号：US16293151

申请日：2019-03-05

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Pin Wang ,  Lili Yang ,  David Baltimore

IPC: C12N15/86 ,  A61K38/17 ,  A61K38/19 ,  A61K38/20 ,  C07K14/005 ,  A61K35/76 ,  A61K39/21 ,  C12N7/00 ,  A61K31/70

Abstract: Methods and compositions are provided for delivery of a polynucleotide encoding a gene of interest, typically an antigen, to a dendritic cell (DC). The virus envelope comprises a DC-SIGN specific targeting molecule. The methods and related compositions can be used to treat patients suffering from a wide range of conditions, including infection, such as HIV/AIDS, and various types of cancers.

公开(公告)号：US10266847B2

公开(公告)日：2019-04-23

申请号：US15820741

申请日：2017-11-22

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Pin Wang ,  Lili Yang ,  David Baltimore

IPC: A01N63/00 ,  A61K48/00 ,  C12N15/86 ,  A61K31/70 ,  A61K38/17 ,  A61K38/19 ,  A61K38/20 ,  C07K14/005 ,  A61K35/76 ,  A61K39/21 ,  C12N7/00 ,  A61K39/00

Abstract: Methods and compositions are provided for delivery of a polynucleotide encoding a gene of interest, typically an antigen, to a dendritic cell (DC). The virus envelope comprises a DC-SIGN specific targeting molecule. The methods and related compositions can be used to treat patients suffering from a wide range of conditions, including infection, such as HIV/AIDS, and various types of cancers.

公开(公告)号：US20190002883A1

公开(公告)日：2019-01-03

申请号：US16028262

申请日：2018-07-05

Applicant: California Institute of Technology ,  The Regents of the University of California

Inventor： Carlos Lois-Caballe ,  David Baltimore ,  Xiao-Feng Qin ,  Irvin S.Y. Chen ,  Dong Sung An

IPC: C12N15/113 ,  C12N15/11 ,  C12N15/63 ,  C12N15/86 ,  A61K48/00

Abstract: In one aspect, the invention provides methods and compositions for the expression of small RNA molecules within a cell using a retroviral vector (FIG. 1A). Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell. In a further aspect, the invention provides methods for producing siRNA encoding lentivirus where the siRNA activity may interfere with the lentiviral life cycle. In yet a further aspect, the invention provides methods for expression of a small RNA molecule within a cell, such as an siRNA capable of downregulating CCR5, wherein expression of the small RNA molecule is relatively non-cytotoxic to the cell. The invention also includes small RNA molecules, such as an siRNA capable of downregulating CCR5, that are relatively non-cytotoxic to cells.

公开(公告)号：US20180187193A1

公开(公告)日：2018-07-05

申请号：US15831839

申请日：2017-12-05

Applicant: California Institute of Technology

Inventor： David Baltimore ,  Ryan M. O'Connell ,  Konstantin Taganov ,  Mark Boldin

IPC: C12N15/113 ,  A61K31/56 ,  A61K31/7105 ,  A61K45/06

CPC classification number: C12N15/113 ,  A61K31/56 ,  A61K31/7105 ,  A61K45/06 ,  C12N2310/11 ,  C12N2310/113 ,  C12N2310/141 ,  C12N2320/31

Abstract: The present disclosure relates to the finding that microRNA-155 plays a role in inflammation, hematopoiesis and myeloproliferation, and that dysregulation of microRNA-155 expression is associated with particular myeloproliferative disorders. Disclosed herein are methods and compositions for diagnosing an treating disorders, including inflammation and myeloproliferation, modulating the levels of expression of one or more genes selected from the group consisting of Cutl1, Arntl, Picalm, Jarid2, PU.1, Csf1r, HIF1α, Sla, Cepbβ, and Bach1, and the like.

公开(公告)号：US09994867B2

公开(公告)日：2018-06-12

申请号：US14338947

申请日：2014-07-23

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： David Baltimore ,  Pin Wang ,  Lili Yang

IPC: A61K39/12 ,  C12N15/867 ,  C07K14/18 ,  C07K16/18 ,  C12N15/86 ,  C07K16/28 ,  A61K39/00

CPC classification number: C12N15/86 ,  A61K2039/505 ,  C07K16/2887 ,  C07K2317/53 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/03 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2740/16045 ,  C12N2799/027 ,  C12N2810/80 ,  C12N2810/851 ,  C12N2810/859 ,  Y02A50/386 ,  Y02A50/396

Abstract: Methods and compositions are provided for delivering a polynucleotide encoding a gene of interest to a target cell using a virus. The virus envelope comprises a cell-specific binding determinant that recognizes and binds to a component on the target cell surface, leading to endocytosis of the virus. A separate fusogenic molecule is also present on the envelope and facilitates delivery of the polynucleotide across the membrane and into the cytosol of the target cell. The methods and related compositions can be used for treating patients having suffering from a wide range of conditions, including infection, such as HIV; cancers, such as non-Hodgkin's lymphoma and breast cancer; and hematological disorders, such as severe combined immunodeficiency.

公开(公告)号：US09963717B2

公开(公告)日：2018-05-08

申请号：US15391189

申请日：2016-12-27

Applicant: California Institute of Technology

Inventor： Carlos Lois-Caballe ,  David Baltimore ,  Xiao-Feng Qin

IPC: C12N15/867 ,  C12N15/63 ,  A61K48/00 ,  C12N15/86 ,  C12N7/00 ,  C12N15/113

CPC classification number: C12N15/86 ,  A61K38/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/113 ,  C12N15/1132 ,  C12N15/1138 ,  C12N15/867 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2310/531 ,  C12N2330/30 ,  C12N2740/15043 ,  C12N2740/16043 ,  C12N2830/003 ,  C12N2830/006 ,  C12N2830/008 ,  C12N2830/30 ,  C12N2830/48 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/20 ,  C12N2840/203 ,  C12Q1/6897 ,  Y02A50/465

Abstract: The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which is capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated.

公开(公告)号：US20160340692A1

公开(公告)日：2016-11-24

申请号：US15051907

申请日：2016-02-24

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Pin Wang ,  Lili Yang ,  David Baltimore

IPC: C12N15/86 ,  A61K35/76 ,  A61K38/17 ,  A61K38/19 ,  A61K38/20 ,  A61K39/21 ,  C12N7/00 ,  C07K14/005

CPC classification number: C12N15/86 ,  A61K31/70 ,  A61K35/76 ,  A61K38/177 ,  A61K38/1774 ,  A61K38/178 ,  A61K38/1793 ,  A61K38/191 ,  A61K38/193 ,  A61K38/20 ,  A61K38/2013 ,  A61K38/2026 ,  A61K38/204 ,  A61K38/2046 ,  A61K38/2086 ,  A61K39/21 ,  A61K2039/5256 ,  C07K14/005 ,  C12N7/00 ,  C12N2740/13043 ,  C12N2740/13045 ,  C12N2740/15041 ,  C12N2740/15043 ,  C12N2740/15045 ,  C12N2770/36122 ,  C12N2770/36134 ,  C12N2810/609 ,  C12N2810/855 ,  Y02A50/386 ,  Y02A50/396 ,  Y02A50/401 ,  Y02A50/403 ,  Y02A50/407 ,  Y02A50/41 ,  Y02A50/412 ,  Y02A50/423 ,  Y02A50/466 ,  Y02A50/475 ,  Y02A50/48 ,  Y02A50/481 ,  Y02A50/492 ,  A61K2300/00

Abstract: Methods and compositions are provided for delivery of a polynucleotide encoding a gene of interest, typically an antigen, to a dendritic cell (DC). The virus envelope comprises a DC-SIGN specific targeting molecule. The methods and related compositions can be used to treat patients suffering from a wide range of conditions, including infection, such as HIV/AIDS, and various types of cancers.