公开(公告)号：US20170334957A1

公开(公告)日：2017-11-23

申请号：US15485338

申请日：2017-04-12

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC分类号： C07K14/415 ,  C12N15/82 ,  C12N15/00

CPC分类号： C12N15/8222 ,  C12N15/00 ,  C12N15/8216 ,  C12N15/8225 ,  C12N15/8241 ,  C12N15/8286 ,  Y02A40/162

摘要： This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US09752155B2

公开(公告)日：2017-09-05

申请号：US14472977

申请日：2014-08-29

申请人： Dow AgroSciences, LLC

发明人： Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

IPC分类号： C12N15/82 ,  C07K14/415

CPC分类号： C12N15/8216 ,  C07K14/415

摘要： Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using the regulatory elements, including the promoters and/or 3′-UTRs, isolated from Brachypodium distachyon ubiquitin genes.

公开(公告)号：US20170081676A1

公开(公告)日：2017-03-23

申请号：US15266370

申请日：2016-09-15

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Sara Bennett ,  Andrew F. Worden

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C12N15/8222 ,  C12N15/8274 ,  C12N15/8286 ,  Y02A40/162

摘要： This disclosure concerns compositions and methods for promoting transcription and translation of a nucleotide sequence in a plant or plant cell, employing a 3′UTR from Arabidopsis thaliana Ubiquitin-10 gene. Some embodiments relate to a 3′ UTR from a Arabidopsis thaliana Ubiquitin-10 gene that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US20170029833A1

公开(公告)日：2017-02-02

申请号：US15212000

申请日：2016-07-15

申请人： Dow AgroSciences LLC

发明人： Sandeep Kumar ,  Diaa Alabed ,  Terry R. Wright ,  Manju Gupta

IPC分类号： C12N15/82 ,  C07K14/005

CPC分类号： C12N15/8216 ,  C07K14/005 ,  C12N15/8222 ,  C12N15/8286 ,  C12N2770/00022 ,  Y02A40/162

摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bidirectional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bidirectional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 aerie or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

摘要翻译： 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包含至少一个与多个基因表达盒的双向启动子连接。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1通气或其功能等同物的最小核心启动子元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US09453235B2

公开(公告)日：2016-09-27

申请号：US13674712

申请日：2012-11-12

申请人： Dow AgroSciences LLC

发明人： Sandeep Kumar ,  Diaa Alabed ,  Sara Bennett ,  Manju Gupta ,  Susan Jayne ,  Terry R. Wright

IPC分类号： C12N15/82

CPC分类号： C12N15/8216 ,  C12N15/8286 ,  Y02A40/162

摘要： Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter linked to multiple gene expression cassettes, wherein the bi-directional promoter comprises a functional promoter nucleotide sequence from Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided employs a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof, and nucleotide sequence elements from a Sugar Cane Bacilliform Virus promoter. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

摘要翻译： 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 所提供的构建体包括至少一个与多个基因表达盒连接的双向启动子，其中双向启动子包含来自甘蔗芽孢杆菌病毒启动子的功能启动子核苷酸序列。 在一些实施方案中，所提供的构建体和方法采用基于来自玉米泛素-1基因或其功能等同物的最小核心启动子元件和来自甘蔗芽孢杆菌病毒启动子的核苷酸序列元件的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US10526610B2

公开(公告)日：2020-01-07

申请号：US15882609

申请日：2018-01-29

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/79 ,  C12N15/82 ,  C12N9/22 ,  C12N15/63

摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.

公开(公告)号：US10400245B2

公开(公告)日：2019-09-03

申请号：US15485338

申请日：2017-04-12

申请人： Dow AgroSciences LLC

发明人： Manju Gupta ,  Sandeep Kumar ,  Wei Chen

IPC分类号： C12N15/82 ,  C12N15/00

摘要： This disclosure concerns compositions and methods for promoting transcription of a nucleotide sequence in a plant or plant cell, employing a Zea mays GRMZM2G047720 promoter. Some embodiments relate to a Zea mays GRMZM2G047720 promoter that functions in plants to promote transcription of operably linked nucleotide sequences. Other embodiments relate to a Zea mays GRMZM2G047720 3′UTR that functions in plants to terminate transcription of operably linked nucleotide sequences.

公开(公告)号：US10344289B2

公开(公告)日：2019-07-09

申请号：US14538609

申请日：2014-11-11

申请人： Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

发明人： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC分类号： C12N15/66 ,  C12N15/62 ,  C12N15/82 ,  C12N9/10

摘要： The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

公开(公告)号：US10030247B2

公开(公告)日：2018-07-24

申请号：US14587750

申请日：2014-12-31

申请人： Dow AgroSciences LLC

发明人： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC分类号： C12N15/82

CPC分类号： C12N15/8216

摘要： The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Z. mays genotype, Z. mays c.v. Hi-II.

公开(公告)号：US20180163217A1

公开(公告)日：2018-06-14

申请号：US15882609

申请日：2018-01-29

申请人： Dow AgroSciences LLC ,  Sangamo Therapeutics, Inc.

发明人： Noel Cogan ,  John Forster ,  Matthew Hayden ,  Tim Sawbridge ,  German Spangenberg ,  Steven R. Webb ,  Manju Gupta ,  W. Michael Ainley ,  Matthew J. Henry ,  Jeffrey C. Miller ,  Dmitry Y. Guschin

IPC分类号： C12N15/79 ,  C12N9/22 ,  C12N15/82 ,  C12N15/63

CPC分类号： C12N15/79 ,  C12N9/22 ,  C12N15/63 ,  C12N15/8241 ,  C12N15/8247

摘要： A method of gene editing or gene stacking within a FAD3 loci by cleaving, in a site directed manner, a location in a FAD3 gene in a cell, to generate a break in the FAD3 gene and then ligating into the break a nucleic acid molecule associated with one or more traits of interest is disclosed.