利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

46 条记录 (耗时 0.049 秒)

    Multiplex nucleic acid amplification
    21.
    发明授权
    Multiplex nucleic acid amplification 失效
    多重核酸扩增

    公开(公告)号：US5736365A

    公开(公告)日：1998-04-07

    申请号：US705225

    申请日：1996-08-29

    申请人： George Terrance Walker ,  James G. Nadeau ,  Patricia Anne Spears ,  Colleen M. Nycz ,  Daryl Dee Shank ,  James L. Schram ,  Stewart Russel Jurgensen

    发明人： George Terrance Walker ,  James G. Nadeau ,  Patricia Anne Spears ,  Colleen M. Nycz ,  Daryl Dee Shank ,  James L. Schram ,  Stewart Russel Jurgensen

    IPC分类号： C12N15/09 ,  C12Q1/04 ,  C12Q1/68 ,  C12R1/32 ,  C12R1/33 ,  C12P19/34 ,  C07H21/04 ,  C12Q1/70

    CPC分类号： C12Q1/689 ,  C12Q1/6851 ,  C12Q2600/16

    摘要： Primers and methods for adapter-mediated multiplex amplification of the IS6110 insertion element of Mycobacterium tuberculosis (M.tb) and the 16S ribosomal gene of Mycobacterium tuberculosis, useful for simultaneously detecting and/or identifying species of the M. tuberculosis complex and other clinically relevant Mycobacterium species. Multiplex Strand Displacement Amplification (SDA) is used in a single amplification reaction which is capable of simultaneously identifying M. tuberculosis and providing a screen for substantially all of the clinically relevant species of Mycobacteria. Also disclosed are methods for adapter-mediated multiplex amplification of multiple target sequences and a single internal control sequence for determination of sample efficacy or quantitation of the targets. In a preferred embodiment, an internal control sequence is included in the amplification reaction and coamplified with the IS6110 and 16S target sequences as an indication of sample amplification activity or to quantitate the initial amount of target sequences in the sample.

    摘要翻译： 针对结核分枝杆菌（M.tb）的IS6110插入元件和结核分枝杆菌的16S核糖体基因的适配器介导的多重扩增的引物和方法，可用于同时检测和/或鉴定结核分枝杆菌复合物和其他临床相关的物种 分枝杆菌种。 多重链位移扩增（SDA）用于能够同时鉴定结核分枝杆菌并为基本上所有临床相关分枝杆菌物种提供筛选的单一扩增反应。 还公开了用于多重靶序列的衔接子介导的多重扩增的方法和用于确定靶的样品功效或定量的单个内部控制序列的方法。 在优选的实施方案中，内部对照序列包括在扩增反应中并与IS6110和16S靶序列共扩增，作为样品扩增活性的指示或定量样品中靶序列的初始量。

    Methods for detecting nucleic acid sequence variations
    24.
    发明授权
    Methods for detecting nucleic acid sequence variations 有权
    检测核酸序列变异的方法

    公开(公告)号：US08323929B2

    公开(公告)日：2012-12-04

    申请号：US12419737

    申请日：2009-04-07

    申请人： Sha-Sha Wang ,  Keith Thornton ,  James G. Nadeau ,  Tobin J. Hellyer

    发明人： Sha-Sha Wang ,  Keith Thornton ,  James G. Nadeau ,  Tobin J. Hellyer

    IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  C07H21/00

    CPC分类号： C12Q1/6827 ,  C12Q2565/1025 ,  C12Q2535/125 ,  C12Q2531/119 ,  C12Q2531/101

    摘要： The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

    摘要翻译： 本发明采用包含5'衔接子序列的未标记信号引物来检测核酸靶序列的变异。 检测系统还包括报道探针，其3'末端与信号引物的5'衔接子序列的互补体杂交以产生5'突出端。 聚合酶用于填充突出端并合成报告基因探针的5'突出端的互补体。 直接或间接检测报道探针补体的合成，作为目标存在的指示。

    Methods for detecting nucleic acid sequence variations
    26.
    发明申请
    Methods for detecting nucleic acid sequence variations 审中-公开
    检测核酸序列变异的方法

    公开(公告)号：US20090131647A1

    公开(公告)日：2009-05-21

    申请号：US11724180

    申请日：2007-03-15

    申请人： James G. Nadeau ,  Tobin J. Hellyer

    发明人： James G. Nadeau ,  Tobin J. Hellyer

    IPC分类号： C07H21/04

    CPC分类号： C12Q1/6827 ,  C12Q2565/1025 ,  C12Q2535/125 ,  C12Q2531/119 ,  C12Q2531/101

    摘要： The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

    摘要翻译： 本发明采用包含5'衔接子序列的未标记信号引物来检测核酸靶序列的变异。 检测系统还包括报道探针，其3'末端与信号引物的5'衔接子序列的互补体杂交以产生5'突出端。 聚合酶用于填充突出端并合成报告基因探针的5'突出端的互补体。 直接或间接检测报道探针补体的合成，作为目标存在的指示。

    Diagnosis of sepsis or SIRS using biomarker profiles
    27.
    发明申请
    Diagnosis of sepsis or SIRS using biomarker profiles 审中-公开
    使用生物标志物图谱诊断败血症或SIRS

    公开(公告)号：US20080138832A1

    公开(公告)日：2008-06-12

    申请号：US11904282

    申请日：2007-09-25

    申请人： Richard M. Ivey ,  Thomas M. Gentle ,  Richard L. Moore ,  Michael L. Towns ,  Nicholas Bachur ,  Robert W. Rosenstein ,  James G. Nadeau ,  Paul E. Goldenbaum ,  Song Shi ,  Donald Copertino ,  James Garrett ,  Gregory Tice

    发明人： Richard M. Ivey ,  Thomas M. Gentle ,  Richard L. Moore ,  Michael L. Towns ,  Nicholas Bachur ,  Robert W. Rosenstein ,  James G. Nadeau ,  Paul E. Goldenbaum ,  Song Shi ,  Donald Copertino ,  James Garrett ,  Gregory Tice

    IPC分类号： G01N33/53 ,  C12Q1/02 ,  G01N33/00

    CPC分类号： G01N33/6803 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2800/26

    摘要： The early prediction or diagnosis of sepsis advantageously allows for clinical intervention before the disease rapidly progresses beyond initial stages to the more severe stages, such as severe sepsis or septic shock, which are associated with high mortality. Early prediction or diagnosis is accomplished by comparing an individual's profile of biomarker expression to profiles obtained from one or more control, or reference, populations, which may include a population that develops sepsis. Recognition of features in the individual's biomarker profile that are characteristic of the onset of sepsis allows a clinician to diagnose the onset of sepsis from a bodily fluid isolated from the individual at a single point in time. The necessity of monitoring the patient over a period of time is, therefore, avoided, advantageously allowing clinical intervention before the onset of serious symptoms of sepsis. Further, because the biomarker expression is assayed for its profile, identification of the particular biomarkers is unnecessary. The comparison of an individual's biomarker profile to biomarker profiles of appropriate reference populations likewise can be used to diagnose SIRS in the individual.

    摘要翻译： 败血症的早期预测或诊断有利地允许在疾病快速进展到初始阶段之前的临床干预到更严重的阶段，例如与高死亡率相关的更严重的败血症或败血性休克。 通过将个体的生物标志物表达谱与从一个或多个对照或参考群体获得的概况进行比较来实现早期预测或诊断，其可包括发生败血症的群体。 识别个体生物标志物特征的特征是脓毒症发作的特征允许临床医生在单个时间点诊断从个体分离的体液的败血症发作。 因此，避免在一段时间内监测患者的必要性，有利地允许在脓毒症严重症状发作之前进行临床干预。 此外，因为生物标志物的表达被测定其特征，所以特定生物标志物的鉴定是不必要的。 个体的生物标记谱与适当参考群的生物标志物概况的比较同样可以用于诊断个体中的SIRS。

    Methods for detecting single nucleotide polymorphisms
    28.
    发明授权
    Methods for detecting single nucleotide polymorphisms 失效
    检测单核苷酸多态性的方法

    公开(公告)号：US07223536B2

    公开(公告)日：2007-05-29

    申请号：US09778168

    申请日：2001-02-07

    申请人： David J. Wright ,  Maria A. Milla ,  James G. Nadeau ,  G. Terrance Walker

    发明人： David J. Wright ,  Maria A. Milla ,  James G. Nadeau ,  G. Terrance Walker

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6827 ,  C12Q2565/1025 ,  C12Q2535/125 ,  C12Q2531/119 ,  C12Q2531/101

    摘要： The present invention provides methods for detecting and identifying sequence variations in a nucleic acid sequence of interest using a detector primer. It has been found that the reduced efficiency of primer extension by DNA polymerases when the 3′ end of a primer does not hybridize perfectly with the target can be adapted for use as a means for distinguishing or identifying the nucleotide in the target which is at the site where the diagnostic mismatch between the detector primer and the target occurs. The detector primer hybridizes to the sequence of interest and is extended with polymerase. The efficiency of detector primer extension is detected as an indication of the presence and/or identity of the sequence variation in the target. The inventive methods make use of nucleotide mismatches at or near the 3′ end of the detector primer to discriminate between the nucleotide sequence of interest and a second nucleotide sequence which may occur at that same site in the target. The methods are particularly well suited for detecting and identifying single nucleotide differences between a target sequence of interest (e.g., a mutant allele of a gene) and a second nucleic acid sequence (e.g., a wild type allele for the same gene).

    摘要翻译： 本发明提供使用检测器引物检测和鉴定感兴趣的核酸序列中的序列变异的方法。 已经发现，当引物的3'端没有与靶完全杂交时，通过DNA聚合酶的引物延伸的效率降低可以适用于区分或鉴定靶中的核苷酸的手段 发现检测器引物与靶标之间的诊断不匹配的位置。 检测引物与感兴趣的序列杂交，并用聚合酶扩增。 检测器引物延伸的效率被检测为目标中序列变异的存在和/或身份的指示。 本发明的方法利用在检测器引物的3'末端附近或其附近的核苷酸错配来区分目的核苷酸序列和可能发生在靶的相同位点的第二核苷酸序列。 所述方法特别适用于检测和鉴定靶标目标序列（例如，基因的突变等位基因）和第二核酸序列（例如，相同基因的野生型等位基因）之间的单核苷酸差异。

    Detection of nucleic acids by fluorescence quenching
    29.
    发明授权
    Detection of nucleic acids by fluorescence quenching 失效

    公开(公告)号：US5935791A

    公开(公告)日：1999-08-10

    申请号：US933749

    申请日：1997-09-23

    申请人： James G. Nadeau ,  Helen V. Hsieh ,  J. Bruce Pitner ,  C. Preston Linn

    发明人： James G. Nadeau ,  Helen V. Hsieh ,  J. Bruce Pitner ,  C. Preston Linn

    IPC分类号： G01N21/64 ,  C07H21/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/542 ,  G01N33/566 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6818

    摘要： Detector nucleic acids are employed for detection of nucleic acid target sequences by fluorescence quenching mechanisms. The detector nucleic acid comprises at least two oligonucleotides and is partially single-stranded and partially double-stranded. One of the two dyes of a donor/acceptor dye pair is linked to the first oligonucleotide and the other is linked to a second oligonucleotide such that they are in close spatial proximity when the first and second oligonucleotides are base-paired and donor fluorescence is quenched. A single second oligonucleotide may be hybridized to the first oligonucleotide or multiple second oligonucleotides may be hybridized to the first oligonucleotide and to each other, forming a junction structure comprising multiple donor/acceptor dye pairs. The detector oligonucleotide retains its partially single-stranded and partially double-stranded conformation in the absence of target. In the presence of target, however, the second oligonucleotide(s) of the detector nucleic acid is/are completely or partially displaced from the first, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence which may be detected as an indication of the presence of the target sequence.

    Detection of nucleic acids by fluorescence quenching
    30.
    发明授权
    Detection of nucleic acids by fluorescence quenching 失效

    公开(公告)号：US5928869A

    公开(公告)日：1999-07-27

    申请号：US865675

    申请日：1997-05-30

    申请人： James G. Nadeau ,  J. Bruce Pitner ,  C. Preston Linn ,  James L. Schram

    发明人： James G. Nadeau ,  J. Bruce Pitner ,  C. Preston Linn ,  James L. Schram

    IPC分类号： G01N33/50 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566 ,  C07H21/00 ,  C07H21/04 ,  C12P19/34

    CPC分类号： C12Q1/6827 ,  C12Q1/6818 ,  C12Q1/6858

    摘要： A detector oligonucleotide having a sequence which forms an intramolecularly base-paired secondary structure is described for use in detecting nucleic acid target sequences and target sequence amplification. The detector oligonucleotide is further modified by linkage to two dyes which form a donor/acceptor dye pair. The two dyes are positioned on the detector oligonucleotide such that they are in close spatial proximity in the base-paired, folded secondary structure, thereby causing quenching of donor fluorescence. The detector oligonucleotide may optionally further comprise a restriction endonuclease recognition site (RERS) which remains partially or entirely single-stranded in the base-paired secondary structure. The RERS is flanked by the two dyes. In the presence of target, the base-paired secondary structure is unfolded or linearized, increasing the distance between the donor and acceptor dyes and causing a change in fluorescence of the donor and/or the acceptor. If an RERS is present, it is rendered double-stranded in the presence of target, allowing cleavage or nicking by a restriction endonuclease and separation of the two dyes onto separate nucleic acid fragments. This may further contribute to the magnitude of the change in fluorescence.