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    Diagnosis of sepsis or SIRS using biomarker profiles
    2.
    发明申请
    Diagnosis of sepsis or SIRS using biomarker profiles 审中-公开
    使用生物标志物图谱诊断败血症或SIRS

    公开(公告)号：US20080138832A1

    公开(公告)日：2008-06-12

    申请号：US11904282

    申请日：2007-09-25

    申请人： Richard M. Ivey ,  Thomas M. Gentle ,  Richard L. Moore ,  Michael L. Towns ,  Nicholas Bachur ,  Robert W. Rosenstein ,  James G. Nadeau ,  Paul E. Goldenbaum ,  Song Shi ,  Donald Copertino ,  James Garrett ,  Gregory Tice

    发明人： Richard M. Ivey ,  Thomas M. Gentle ,  Richard L. Moore ,  Michael L. Towns ,  Nicholas Bachur ,  Robert W. Rosenstein ,  James G. Nadeau ,  Paul E. Goldenbaum ,  Song Shi ,  Donald Copertino ,  James Garrett ,  Gregory Tice

    IPC分类号： G01N33/53 ,  C12Q1/02 ,  G01N33/00

    CPC分类号： G01N33/6803 ,  C12Q1/6837 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q2600/158 ,  G01N2800/26

    摘要： The early prediction or diagnosis of sepsis advantageously allows for clinical intervention before the disease rapidly progresses beyond initial stages to the more severe stages, such as severe sepsis or septic shock, which are associated with high mortality. Early prediction or diagnosis is accomplished by comparing an individual's profile of biomarker expression to profiles obtained from one or more control, or reference, populations, which may include a population that develops sepsis. Recognition of features in the individual's biomarker profile that are characteristic of the onset of sepsis allows a clinician to diagnose the onset of sepsis from a bodily fluid isolated from the individual at a single point in time. The necessity of monitoring the patient over a period of time is, therefore, avoided, advantageously allowing clinical intervention before the onset of serious symptoms of sepsis. Further, because the biomarker expression is assayed for its profile, identification of the particular biomarkers is unnecessary. The comparison of an individual's biomarker profile to biomarker profiles of appropriate reference populations likewise can be used to diagnose SIRS in the individual.

    摘要翻译： 败血症的早期预测或诊断有利地允许在疾病快速进展到初始阶段之前的临床干预到更严重的阶段，例如与高死亡率相关的更严重的败血症或败血性休克。 通过将个体的生物标志物表达谱与从一个或多个对照或参考群体获得的概况进行比较来实现早期预测或诊断，其可包括发生败血症的群体。 识别个体生物标志物特征的特征是脓毒症发作的特征允许临床医生在单个时间点诊断从个体分离的体液的败血症发作。 因此，避免在一段时间内监测患者的必要性，有利地允许在脓毒症严重症状发作之前进行临床干预。 此外，因为生物标志物的表达被测定其特征，所以特定生物标志物的鉴定是不必要的。 个体的生物标记谱与适当参考群的生物标志物概况的比较同样可以用于诊断个体中的SIRS。

    Sequence-specific methods for homogeneous, real-time detection of lamp products
    6.
    发明授权
    Sequence-specific methods for homogeneous, real-time detection of lamp products 有权
    灯具产品均匀，实时检测的序列特异性方法

    公开(公告)号：US09315863B2

    公开(公告)日：2016-04-19

    申请号：US13505598

    申请日：2010-11-04

    申请人： James G. Nadeau

    发明人： James G. Nadeau

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6865 ,  C12Q2525/307

    摘要： Presented herein are methods and compositions for generating sequence-specific, secondary amplification products during Loop-mediated Isothermal Amplification (LAMP). Conventional LAMP produces a preponderance of high molecular weight DNA structures concatenated into self-complementary hairpins, which are not amenable to detection by routine probe-based hybridization methods, making multiplex detection of two or more targets or sequence variants in closed-tube formats extremely difficult. Provided herein, for example, are methods for generating secondary LAMP products bearing a fragment of the original target sequence embedded within low-molecular weight products that are devoid of competitive hairpin structures, the lack of which enhances probe-based detection of target sequences. These secondary products can, for example, be produced in real-time, during the LAMP process, and can provide the option of detecting multiple target sequences within a single tube using, e.g., a homogenous, real-time fluorescence format.

    摘要翻译： 本文提出了在环介导的等温扩增（LAMP）期间产生序列特异性二级扩增产物的方法和组合物。 常规的LAMP产生连接到自身互补发夹中的高分子量DNA结构的优势，其不适于通过常规基于探针的杂交方法的检测，使得封闭管形式中的两个或更多个目标或序列变体的多重检测非常困难 。 例如，本文提供的是产生具有嵌入低分子量产物中的原始靶序列的片段的次级LAMP产物的方法，所述片段不含有竞争力的发夹结构，其缺乏可增强靶序列的基于探针的检测。 这些次要产物可以例如在LAMP过程期间实时产生，并且可以提供使用例如均匀的实时荧光格式在单个管内检测多个靶序列的选项。

    Simultaneous amplification of multiple targets
    9.
    发明授权
    Simultaneous amplification of multiple targets 失效
    同时放大多个目标

    公开(公告)号：US5422252A

    公开(公告)日：1995-06-06

    申请号：US73197

    申请日：1993-06-04

    申请人： George T. Walker ,  James G. Nadeau ,  Michael C. Little

    发明人： George T. Walker ,  James G. Nadeau ,  Michael C. Little

    IPC分类号： G01N33/566 ,  C07H21/04 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G11B5/00 ,  G11B5/09 ,  G11B5/187 ,  G11B5/31 ,  G11B5/455 ,  G11B5/465 ,  G11B19/04 ,  C12Q1/70

    CPC分类号： C12Q1/6853

    摘要： Methods for multiplex amplification of target nucleic acid sequences using a single pair of primers. Defined sequences are appended to the ends of multiple target sequences as part of the amplification reaction so that no steps in addition to amplification are required. The target sequences with the appended defined sequences need not be isolated prior to amplification. In one embodiment for coamplification of two target sequences, a sequence corresponding to a terminal segment of the first target sequence is appended to one end of the second target sequence and a sequence corresponding to a terminal segment of the second target sequence is appended to one end of the first target sequence. Amplification of the two targets then requires only a single pair of primers. Alternatively, a single defined sequence may be appended to the 5' and 3' ends of any number of selected targets. All such modified target sequences may then be amplified using a single pair of primers which hybridize to the defined end-sequences.

    摘要翻译： 使用单对引物多重扩增靶核酸序列的方法。 定义的序列作为扩增反应的一部分附加到多个靶序列的末端，因此不需要扩增步骤。 具有附加定义序列的靶序列不需要在扩增前分离。 在两个目标序列的共放大的一个实施例中，对应于第一目标序列的末端片段的序列被附加到第二目标序列的一端，并且与第二目标序列的末端片段相对应的序列被附加到一端 的第一个靶序列。 两个靶标的扩增只需要一对引物即可。 或者，单个定义的序列可以附加到任意数目的所选目标的5'和3'端。 然后可以使用与定义的末端序列杂交的单对引物扩增所有这些修饰的靶序列。

    METHODS FOR DETECTING NUCLEIC ACID SEQUENCE VARIATIONS
    10.
    发明申请
    METHODS FOR DETECTING NUCLEIC ACID SEQUENCE VARIATIONS 有权
    检测核酸序列变异的方法

    公开(公告)号：US20090246792A1

    公开(公告)日：2009-10-01

    申请号：US12419737

    申请日：2009-04-07

    申请人： Sha-Sha WANG ,  Keith Thornton ,  James G. Nadeau ,  Tobin J. Hellyer

    发明人： Sha-Sha WANG ,  Keith Thornton ,  James G. Nadeau ,  Tobin J. Hellyer

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6827 ,  C12Q2565/1025 ,  C12Q2535/125 ,  C12Q2531/119 ,  C12Q2531/101

    摘要： The invention employs an unlabeled signal primer comprising a 5′ adapter sequence for detection of variations in nucleic acid target sequences. The detection system further comprises a reporter probe, the 3′ end of which hybridizes to the complement of the 5′ adapter sequence of the signal primer to produce a 5′ overhang. Polymerase is used to fill in the overhang and synthesize the complement of the 5′ overhang of the reporter probe. Synthesis of the reporter probe complement is detected, either directly or indirectly, as an indication of the presence of the target.

    摘要翻译： 本发明采用包含5'衔接子序列的未标记信号引物来检测核酸靶序列的变异。 检测系统还包括报道探针，其3'末端与信号引物的5'衔接子序列的互补体杂交以产生5'突出端。 聚合酶用于填充突出端并合成报告基因探针的5'突出端的互补体。 直接或间接检测报道探针补体的合成，作为目标存在的指示。