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39 条记录 (耗时 0.128 秒)

    Nucleotide sequence of the Haemophilus influenzae Rd genome, fragments thereof, and uses thereof
    23.
    发明授权
    Nucleotide sequence of the Haemophilus influenzae Rd genome, fragments thereof, and uses thereof 失效
    流感嗜血杆菌Rd基因组的核苷酸序列，其片段及其用途

    公开(公告)号：US06506581B1

    公开(公告)日：2003-01-14

    申请号：US09557884

    申请日：2000-04-25

    申请人： Robert D. Fleischmann ,  Mark D. Adams ,  Owen White ,  Hamilton O. Smith ,  J. Craig Venter

    发明人： Robert D. Fleischmann ,  Mark D. Adams ,  Owen White ,  Hamilton O. Smith ,  J. Craig Venter

    IPC分类号： C12N121

    CPC分类号： C07K14/285 ,  Y10S435/851

    摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO:1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.

    摘要翻译： 本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。 本发明还提供存储在计算机可读介质上的序列信息，以及便于其使用的基于计算机的系统和方法。 除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件 基因组

    Computer readable genomic sequence of Haemophilus influenzae Rd, fragments thereof, and uses thereof
    24.
    发明授权
    Computer readable genomic sequence of Haemophilus influenzae Rd, fragments thereof, and uses thereof 失效
    流感嗜血杆菌Rd的计算机可读基因组序列及其用途及其用途

    公开(公告)号：US06355450B1

    公开(公告)日：2002-03-12

    申请号：US08476102

    申请日：1995-06-07

    申请人： Robert D. Fleischmann ,  Mark D. Adams ,  Owen White ,  Hamilton O. Smith ,  J. Craig Venter

    发明人： Robert D. Fleischmann ,  Mark D. Adams ,  Owen White ,  Hamilton O. Smith ,  J. Craig Venter

    IPC分类号： C12P2106

    CPC分类号： C07K14/285 ,  Y10S435/851

    摘要： The present invention provides the sequencing of the entire genome of Haemophilus influenzae Rd, SEQ ID NO: 1. The present invention further provides the sequence information stored on computer readable media, and computer-based systems and methods which facilitate its use. In addition to the entire genomic sequence, the present invention identifies over 1700 protein encoding fragments of the genome and identifies, by position relative to a unique Not I restriction endonuclease site, any regulatory elements which modulate the expression of the protein encoding fragments of the Haemophilus genome.

    摘要翻译： 本发明提供了流感嗜血杆菌Rd，SEQ ID NO：1的整个基因组的测序。本发明还提供存储在计算机可读介质上的序列信息以及便于其使用的基于计算机的系统和方法。 除了整个基因组序列之外，本发明鉴定了基因组的超过1700个蛋白质编码片段，并且通过相对于独特的Not I限制性内切核酸酶位点的位置鉴定调节嗜血杆菌蛋白编码片段的表达的任何调节元件 基因组

    Cloned cell line expressing rat .beta..sub.3A adrenergic receptor
    25.
    发明授权
    Cloned cell line expressing rat .beta..sub.3A adrenergic receptor 失效
    克隆细胞株表达大鼠β3A肾上腺素能受体

    公开(公告)号：US5418160A

    公开(公告)日：1995-05-23

    申请号：US783602

    申请日：1991-11-01

    申请人： J. Craig Venter ,  Claire M. Fraser ,  Jean-Paul Giacobino

    发明人： J. Craig Venter ,  Claire M. Fraser ,  Jean-Paul Giacobino

    IPC分类号： C07K14/705 ,  C12N5/10

    CPC分类号： C07K14/70571

    摘要： The present invention relates to a fat cell specific rat .beta.-adrenergic receptor that mediates lipolysis in rats. The invention further relates to cloned cells which code for the specific .beta.-adrenergic receptor that mediates lipolysis. Another aspect of the present invention relates to a diagnostic test method for determining decreased levels of fat cell .beta.-adrenergic receptors that mediate lipotysis in order to diagnosis obesity caused by less active lipolysis.

    摘要翻译： 本发明涉及介导大鼠脂肪分解的脂肪细胞特异性大鼠β-肾上腺素能受体。 本发明还涉及编码介导脂肪分解的特异性β-肾上腺素能受体的克隆细胞。 本发明的另一方面涉及用于确定介导脂质分解的脂肪细胞β-肾上腺素能受体水平降低的诊断测试方法，以便诊断由较不活跃的脂肪分解引起的肥胖。

    Method of nucleic acid cassette assembly
    26.
    发明授权
    Method of nucleic acid cassette assembly 有权

    公开(公告)号：US10041060B2

    公开(公告)日：2018-08-07

    申请号：US11635355

    申请日：2006-12-06

    申请人： J. Craig Venter ,  Hamilton O. Smith ,  Clyde A. Hutchison, III ,  Daniel G. Gibson

    发明人： J. Craig Venter ,  Hamilton O. Smith ,  Clyde A. Hutchison, III ,  Daniel G. Gibson

    IPC分类号： C12Q1/68 ,  C12N15/10 ,  C12N15/66 ,  C07H21/02

    摘要： Methods are provided for constructing a synthetic genome, comprising generating and assembling nucleic acid cassettes comprising portions of the genome, wherein at least one of the nucleic acid cassettes is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. In one embodiment, the entire synthetic genome is constructed from nucleic acid components that have been chemically synthesized, or from copies of the chemically synthesized nucleic acid components. Rational methods may be used to design the synthetic genome (e.g., to establish a minimal genome and/or to optimize the function of genes within a genome, such as by mutating or rearranging the order of the genes). Synthetic genomes of the invention may be introduced into vesicles (e.g., bacterial cells from which part or all of the resident genome has been removed, or synthetic vesicles) to generate synthetic cells. Synthetic genomes or synthetic cells may be used for a variety of purposes, including the generation of synthetic fuels, such as hydrogen or ethanol.

    Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
    29.
    发明授权
    Methods for in vitro joining and combinatorial assembly of nucleic acid molecules 有权
    核酸分子的体外连接和组合装配方法

    公开(公告)号：US08968999B2

    公开(公告)日：2015-03-03

    申请号：US12371543

    申请日：2009-02-13

    申请人： Daniel G. Gibson ,  Hamilton O. Smith ,  Clyde A. Hutchison ,  Lei Young ,  J. Craig Venter

    发明人： Daniel G. Gibson ,  Hamilton O. Smith ,  Clyde A. Hutchison ,  Lei Young ,  J. Craig Venter

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/02 ,  C12N15/66 ,  C12N15/10 ,  C12N15/64

    CPC分类号： C12P19/34 ,  C12N15/10 ,  C12N15/1027 ,  C12N15/64 ,  C12N15/66

    摘要： The present invention relates to methods of joining two or more double-stranded (ds) or single-stranded (ss) DNA molecules of interest in vitro, wherein the distal region of the first DNA molecule and the proximal region of the second DNA molecule of each pair share a region of sequence identity. The method allows the joining of a large number of DNA fragments, in a predetermined order and orientation, without the use of restriction enzymes. It can be used, e.g., to join synthetically produced sub-fragments of a gene or genome of interest. Kits for performing the method are also disclosed. The methods of joining DNA molecules may be used to generate combinatorial libraries useful to generate, for example, optimal protein expression through codon optimization, gene optimization, and pathway optimization.

    摘要翻译： 本发明涉及在体外连接两个或多个目的双链（ds）或单链（ss）DNA分子的方法，其中第一DNA分子的远端区域和第二DNA分子的近端区域 每对共享序列同一性区域。 该方法允许以预定顺序和取向连接大量DNA片段，而不使用限制酶。 其可以用于例如合成产生的感兴趣的基因或基因组的亚片段。 还公开了用于执行该方法的套件。 连接DNA分子的方法可用于产生组合文库，其可用于产生例如通过密码子优化，基因优化和途径优化的最佳蛋白质表达。