-
公开(公告)号:US20140243224A1
公开(公告)日:2014-08-28
申请号:US13787396
申请日:2013-03-06
Applicant: ILLUMINA, INC.
Inventor: Steven M. Barnard , M. Shane Bowen , Maria Candelaria Rogert Bacigalupo , Wayne N. George , Andrew A. Brown , James Tsay
CPC classification number: B01J19/0046 , B01J2219/00317 , B01J2219/00621 , B01J2219/00644 , B01J2219/00722 , C12N15/1068 , C12Q1/6837 , C12Q2563/159
Abstract: Provided is an array including a solid support having a surface, the surface having a plurality of wells, the wells containing a gel material, the wells being separated from each other by interstitial regions on the surface, the interstitial regions segregating the gel material in each of the wells from the gel material in other wells of the plurality; and a library of target nucleic acids in the gel material, wherein the gel material in each of the wells comprises a single species of the target nucleic acids of the library. Methods for making and using the array are also provided.
Abstract translation: 提供了一种阵列,其包括具有表面的固体支持物,所述表面具有多个孔,所述孔包含凝胶材料,所述孔通过表面上的间隙区彼此分离,所述间隙区域在每个孔中分离凝胶材料 的来自多个其他孔中的凝胶材料的孔; 和凝胶材料中的靶核酸文库,其中每个孔中的凝胶材料包含文库的单个靶核酸的种类。 还提供了制作和使用阵列的方法。
-
公开(公告)号:US20140200158A1
公开(公告)日:2014-07-17
申请号:US14194143
申请日:2014-02-28
Applicant: Illumina, Inc.
Inventor: M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
IPC: C12Q1/68
CPC classification number: C12Q1/6844 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00722 , B01J2219/00788 , C12Q1/686 , C40B50/18 , C12Q2527/125 , C12Q2535/122 , C12Q2565/543
Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
Abstract translation: 设计的微阵列在基底上的多个位点的每一个处捕获一个或多个感兴趣的分子。 这些部位包括促进分子在部位附着的基底垫,例如聚合物基底垫。 微阵列可以通过一种或多种图案化技术制成,以产生所需图案中的基底布局。 此外,微阵列可以包括在站点鼓励克隆性的特征。
-