公开(公告)号：US20250027152A1

公开(公告)日：2025-01-23

申请号：US18905923

申请日：2024-10-03

Applicant: Illumina, Inc.

Inventor： Andrew A. Brown ,  Wayne N. George ,  Alexandre Richez ,  M. Shane Bowen

IPC: C12Q1/6874 ,  B01L3/00 ,  G01N21/64 ,  G03F7/00 ,  G03F7/16

Abstract: Substrates comprising dual functional polymer layered surfaces and the preparation thereof by using UV nano-imprinting processes are disclosed. The substrates can be used as flow cells, nanofluidic or microfluidic devices for biological molecules analysis.

公开(公告)号：US11932900B2

公开(公告)日：2024-03-19

申请号：US17973327

申请日：2022-10-25

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Alexandre Richez ,  M. Shane Bowen ,  Andrew A. Brown ,  Dajun Yuan ,  Audrey Rose Zak ,  Sean M. Ramirez ,  Raymond Campos

IPC: C08J7/04 ,  C09D133/26 ,  C09D183/06 ,  C12Q1/68 ,  C08G77/14

CPC classification number: C12Q1/68 ,  C09D133/26 ,  C09D183/06 ,  C08G77/14

Abstract: An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.

公开(公告)号：US20230357846A1

公开(公告)日：2023-11-09

申请号：US18324708

申请日：2023-05-26

Applicant: Illumina, Inc.

Inventor： Wayne N. George ,  Andrew A. Brown ,  Daniel Bratton ,  Hongji Ren ,  Ryan Christopher Smith

IPC: C12Q1/6874 ,  B05D3/06 ,  C09D133/26 ,  C09D135/00 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  B05D3/065 ,  B05D3/06 ,  C09D133/26 ,  C09D135/00 ,  C12Q1/6806 ,  Y10T428/24917 ,  Y10T428/24802 ,  Y10T428/24926 ,  Y10T428/2998 ,  Y10T428/31536 ,  Y10T428/31935

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

公开(公告)号：US20230250248A1

公开(公告)日：2023-08-10

申请号：US18190685

申请日：2023-03-27

Applicant: Illumina, Inc.

Inventor： Lorenzo Berti ,  Andrew A. Brown ,  Wayne N. George

IPC: C08J7/12 ,  C12Q1/6876 ,  C08F8/00 ,  C12Q1/6874 ,  B01J19/00 ,  C09D133/26 ,  C12Q1/6837

CPC classification number: C08J7/12 ,  C12Q1/6876 ,  C08F8/00 ,  C12Q1/6874 ,  B01J19/0046 ,  C09D133/26 ,  C12Q1/6837 ,  C08J2335/00 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C08F220/603

Abstract: Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.

公开(公告)号：US11384392B2

公开(公告)日：2022-07-12

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210024991A1

公开(公告)日：2021-01-28

申请号：US16626452

申请日：2019-06-07

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/6869 ,  G03F7/075 ,  C08G77/04 ,  C08F220/56

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20200025670A1

公开(公告)日：2020-01-23

申请号：US16515849

申请日：2019-07-18

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Timothy J. Merkel ,  Wayne N. George ,  Andrew A. Brown ,  Audrey Zak ,  Gianluca Andrea Artioli ,  Julia Morrison ,  Nikolai Romanov ,  Lorenzo Berti ,  Graham Boud

IPC: G01N15/14 ,  C08L83/12 ,  G03F7/00

Abstract: An example of a resin composition includes an epoxy resin matrix, a free radical photoinitiator selected from the group consisting of 2-ethyl-9,10-dimethoxyanthracene, 2,2-dimethoxy-2-phenylacetophenone, 2-ethoxy-2-phenylacetophenone, and a phosphine oxide, and a photoacid generator. When cured, the resin composition has low or no autofluorescence when exposed to blue excitation wavelengths ranging from about 380 nm to about 480 nm or green excitation wavelengths ranging from about 510 nm to about 560 nm.

公开(公告)号：US20180178416A1

公开(公告)日：2018-06-28

申请号：US15847150

申请日：2017-12-19

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Alexandre Richez ,  Andrew A. Brown ,  Julia Morrison ,  Wayne N. George ,  Timothy J. Merkel ,  Audrey Rose Zak

IPC: B29C33/38 ,  C09D4/00 ,  C09D133/26 ,  B29C33/40 ,  B29C59/02 ,  G03F7/00

Abstract: An imprinting apparatus includes a silicon master having a plurality of nanofeatures defined therein. An anti-stick layer coats the silicon master, the anti-stick layer including a molecule having a cyclosiloxane with at least one silane functional group. A method includes forming a master template by: depositing a formulation on a silicon master including a plurality of nanofeatures defined therein, the formulation including a solvent and a molecule having a cyclosiloxane with at least one silane functional group; and curing the formulation, thereby forming an anti-stick layer on the silicon master, the anti-stick layer including the molecule. The method further includes depositing a silicon-based working stamp material on the anti-stick layer of the master template; curing the silicon-based working stamp material to form a working stamp including a negative replica of the plurality of nanofeatures; and releasing the working stamp from the master template.

公开(公告)号：US20250114980A1

公开(公告)日：2025-04-10

申请号：US18964377

申请日：2024-11-30

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Alexandre Richez ,  Andrew A. Brown ,  Julia Morrison ,  Wayne N. George ,  Timothy J. Merkel ,  Audrey Rose Zak

IPC: B29C33/38 ,  B29C33/40 ,  B29C59/02 ,  B29L31/00 ,  B81C1/00 ,  C09D4/00 ,  C09D133/26 ,  G03F7/00

Abstract: An imprinting apparatus includes a silicon master having a plurality of nanofeatures defined therein. An anti-stick layer coats the silicon master, the anti-stick layer including a molecule having a cyclosiloxane with at least one silane functional group. A method includes forming a master template by: depositing a formulation on a silicon master including a plurality of nanofeatures defined therein, the formulation including a solvent and a molecule having a cyclosiloxane with at least one silane functional group; and curing the formulation, thereby forming an anti-stick layer on the silicon master, the anti-stick layer including the molecule. The method further includes depositing a silicon-based working stamp material on the anti-stick layer of the master template; curing the silicon-based working stamp material to form a working stamp including a negative replica of the plurality of nanofeatures; and releasing the working stamp from the master template.

公开(公告)号：US20250010285A1

公开(公告)日：2025-01-09

申请号：US18750789

申请日：2024-06-21

Applicant: ILLUMINA, INC.

Inventor： Wayne N. George ,  Iuliana Petruta Maria ,  Randall Smith ,  Andrew A. Brown

IPC: B01L3/00 ,  C07D257/08 ,  C07F9/6561

Abstract: An example of a flow cell includes a substrate including a surface and a dendron architecture. The dendron architecture includes a functionalized focal point of attachment that is attached to the substrate surface and a plurality of peripheral functional groups that are orthogonal to the functionalized focal point of attachment. The flow cell further includes a primer set attached to the dendron architecture via the plurality of peripheral functional groups.