公开(公告)号：US20210208055A1

公开(公告)日：2021-07-08

申请号：US17191612

申请日：2021-03-03

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  B01J19/00

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20230116852A1

公开(公告)日：2023-04-13

申请号：US17813852

申请日：2022-07-20

Applicant: Illumina, Inc.

Inventor： Hayden Black ,  Mathieu Lessard-Viger ,  James Tsay

IPC: C12N15/10 ,  C12Q1/6806

Abstract: Embodiments of the present disclosure relate to method of preparing a substrate for sequencing by synthesis, including capturing library DNA to the surface using a low salt buffer solution prior to grafting primer oligonucleotides. Substrates prepared by the method described herein have increased monoclonality of clusters and sequencing by synthesis using the substrate prepared by the method are also described.

公开(公告)号：US11060135B2

公开(公告)日：2021-07-13

申请号：US16118268

申请日：2018-08-30

Applicant: ILLUMINA, INC.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: C12Q1/6844 ,  C40B50/18 ,  C12Q1/686 ,  B01J19/00 ,  C40B40/06

Abstract: A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.

公开(公告)号：US20180195950A1

公开(公告)日：2018-07-12

申请号：US15848450

申请日：2017-12-20

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20130116153A1

公开(公告)日：2013-05-09

申请号：US13661524

申请日：2012-10-26

Applicant: ILLUMINA, INC.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: B01J19/00

CPC classification number: C12Q1/6844 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00662 ,  B01J2219/00722 ,  B01J2219/00788 ,  C12Q1/686 ,  C40B50/18 ,  C12Q2527/125 ,  C12Q2535/122 ,  C12Q2565/543

Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.

Abstract translation: 设计的微阵列在基底上的多个位点的每一个处捕获一个或多个感兴趣的分子。 这些部位包括促进分子在部位附着的基底垫，例如聚合物基底垫。 微阵列可以通过一种或多种图案化技术制成，以产生所需图案中的基底布局。 此外，微阵列可以包括在站点鼓励克隆性的特征。

公开(公告)号：US11466321B2

公开(公告)日：2022-10-11

申请号：US16471411

申请日：2017-12-20

Applicant: ILLUMINA, INC.

Inventor： James Tsay ,  Yuxiang Huang

IPC: C12Q1/6874 ,  B01L3/00 ,  C40B40/06

Abstract: An example of an array includes a support including a plurality of discrete wells, a gel material positioned in each of the discrete wells, a sequencing primer grafted to the gel material, and a non-sequencing entity grafted to the gel material. Each of the sequencing primer and the non-sequencing entity is in its as-grafted form.

公开(公告)号：US11173466B2

公开(公告)日：2021-11-16

申请号：US16118307

申请日：2018-08-30

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: B01J19/00 ,  C12N15/10 ,  C12Q1/6837

Abstract: An example method includes contacting a substrate coated with a sol-gel material with a stamp that includes a plurality of protruding features. While contacting the coated sol-gel material with the stamp, the example method further includes curing the coated sol-gel material so as to form a patterned sol-gel layer that includes a plurality of wells. The stamp is separated from the patterned sol-gel layer.

公开(公告)号：US20210108258A1

公开(公告)日：2021-04-15

申请号：US17113180

申请日：2020-12-07

Applicant: ILLUMINA, INC.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: C12Q1/6844 ,  C40B50/18 ,  C12Q1/686 ,  B01J19/00

Abstract: A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.

公开(公告)号：US10955332B2

公开(公告)日：2021-03-23

申请号：US15848450

申请日：2017-12-20

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  B01J19/00 ,  G01N15/10

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20240117418A1

公开(公告)日：2024-04-11

申请号：US18507470

申请日：2023-11-13

Applicant: ILLUMINA, INC.

Inventor： M. Shane Bowen ,  Kevin L. Gunderson ,  Shengrong Lin ,  Maria Candelaria Rogert Bacigalupo ,  Kandaswamy Vijayan ,  Yir-Shyuan Wu ,  Bala Murali Venkatesan ,  James Tsay ,  John M. Beierle ,  Lorenzo Berti ,  Sang Ryul Park

IPC: C12Q1/6844 ,  B01J19/00 ,  C12Q1/686 ,  C40B50/18

CPC classification number: C12Q1/6844 ,  B01J19/0046 ,  C12Q1/686 ,  C40B50/18 ,  C40B40/06

Abstract: A microarray is designed to capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.