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公开(公告)号：US5837459A
公开(公告)日：1998-11-17
申请号：US653605
申请日：1996-05-24
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68 , G01N33/53 , G01N33/566
CPC分类号： C12P19/34 , C12Q1/6813 , C12Q1/6865
摘要： RNA is transcribed from a double stranded DNA template by forming a complex by hybridizing to the template at a desired transcription initiation site one or more oligonucleic acid analogues of the PNA type capable of forming a transcription initiation site with the DNA and exposing the complex to the action of a DNA dependant RNA polymerase in the presence of nucleoside triphosphates. Equal length transcripts may be obtained by placing a block to transcription downstream from the initiation site or by cutting the template at such a selected location. The initiation site is formed by displacement of one strand of the DNA locally by the PNA hybridization.
摘要翻译： 通过在期望的转录起始位点与模板杂交形成复合物，RNA可以从双链DNA模板转录成能够与DNA形成转录起始位点的一种或多种PNA型寡核酸类似物，并将该复合物暴露于 在DNA核苷三磷酸存在下DNA依赖性RNA聚合酶的作用。 可以通过将起始位点的下游位置嵌段转移到转录起始位点或通过在这样的选择位置切割模板来获得相等的长度转录物。 通过PNA杂交局部置换DNA的一条链来形成起始位点。
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公开(公告)号：US5719262A
公开(公告)日：1998-02-17
申请号：US685484
申请日：1996-07-24
申请人： Ole Buchardt, deceased , by Dorte Buchardt, legal representative , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg
发明人： Ole Buchardt, deceased , by Dorte Buchardt, legal representative , Michael Egholm , Peter Eigil Nielsen , Rolf Henrik Berg
CPC分类号： C07H21/00 , C07K14/003 , C07K5/06139 , C12Q1/68 , C12Q1/6869
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary DNA and RNA strands more strongly than the corresponding DNA or RNA strands, and exhibit increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases attached to a polyamide backbone, and contain alkylamine side chains.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA或RNA链更强地结合互补DNA和RNA链，并表现出增加的序列特异性和溶解度。 肽核酸包含选自天然存在的核碱基和连接到聚酰胺主链的非天然存在的核碱基并且含有烷基胺侧链的配体。
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公开(公告)号：US5539082A
公开(公告)日：1996-07-23
申请号：US54363
申请日：1993-04-26
申请人： Peter E. Nielsen , Ole Buchardt , Michael Egholm , Rolf H. Berg
发明人： Peter E. Nielsen , Ole Buchardt , Michael Egholm , Rolf H. Berg
IPC分类号： C12N15/09 , A61K31/00 , A61K31/52 , A61K31/522 , A61K31/70 , A61K31/7088 , A61K38/00 , A61K48/00 , A61P31/00 , A61P31/12 , A61P35/00 , C07C233/46 , C07C237/22 , C07D239/24 , C07D239/54 , C07D473/18 , C07D473/34 , C07H21/00 , C07K5/06 , C07K7/06 , C07K7/08 , C07K14/00 , C08G69/08 , C12N15/113 , C12Q1/68 , C07K7/00 , C07D239/00 , C07H5/04 , C07H21/04
CPC分类号： C12N15/113 , B82Y5/00 , C07H21/00 , C07K14/003 , C07K5/06026 , C07K7/06 , C07K7/08 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , A61K38/00 , C12N2310/15 , C12N2310/3181
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
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24.发明申请公开(公告)号：US20120264632A1
公开(公告)日：2012-10-18
申请号：US13443629
申请日：2012-04-10
申请人： John Harris Leamon , William Lun Lee , Jan Fredrik Simons , Brian Desany , Michael Todd Ronan , James Drake , Kenton Lohman , Michael Egholm , Jonathan Rothberg
发明人： John Harris Leamon , William Lun Lee , Jan Fredrik Simons , Brian Desany , Michael Todd Ronan , James Drake , Kenton Lohman , Michael Egholm , Jonathan Rothberg
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2565/301 , C12Q2531/107 , C12Q2531/101 , C12Q2565/537 , C12Q2565/515 , C12Q2545/114
摘要： The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.
摘要翻译： 所要求保护的发明提供了使用焦磷酸测序技术直接测序PCR产物的新的样品制备方法。 PCR产物可以是基因组的特定区域。 本公开中提供的技术允许在一个个体或个体群体中的单个等位基因多态性的SNP（单核苷酸多态性）检测，分类和评估。 结果可用于患者的诊断和治疗以及病毒和细菌群体鉴定的评估。
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25.发明申请公开(公告)号：US20060228721A1
公开(公告)日：2006-10-12
申请号：US11104781
申请日：2005-04-12
申请人： John Leamon , William Lee , Jan Simons , Brian Desany , Michael Ronan , James Drake , Kenton Lohman , Michael Egholm , Jonathan Rothberg
发明人： John Leamon , William Lee , Jan Simons , Brian Desany , Michael Ronan , James Drake , Kenton Lohman , Michael Egholm , Jonathan Rothberg
CPC分类号： C12Q1/6827 , C12Q1/6834 , C12Q1/6858 , C12Q2565/301 , C12Q2531/107 , C12Q2531/101 , C12Q2565/537 , C12Q2565/515 , C12Q2545/114
摘要： The claimed invention provides for new sample preparation methods enabling direct sequencing of PCR products using pyrophosphate sequencing techniques. The PCR products may be specific regions of a genome. The techniques provided in this disclosure allows for SNP (single nucleotide polymorphism) detection, classification, and assessment of individual allelic polymorphisms in one individual or a population of individuals. The results may be used for diagnostic and treatment of patients as well as assessment of viral and bacterial population identification.
摘要翻译： 所要求保护的发明提供了使用焦磷酸测序技术直接测序PCR产物的新的样品制备方法。 PCR产物可以是基因组的特定区域。 本公开中提供的技术允许在一个个体或个体群体中的单个等位基因多态性的SNP（单核苷酸多态性）检测，分类和评估。 结果可用于患者的诊断和治疗以及病毒和细菌群体鉴定的评估。
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公开(公告)号：US20060160731A1
公开(公告)日：2006-07-20
申请号：US10691012
申请日：2003-10-22
申请人： Ole Buchardt , Michael Egholm , Peter Nielsen , Rolf Berg
发明人： Ole Buchardt , Michael Egholm , Peter Nielsen , Rolf Berg
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
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公开(公告)号：US20050048552A1
公开(公告)日：2005-03-03
申请号：US10909914
申请日：2004-08-02
申请人： David Ecker , Ole Buchardt , Michael Egholm , Peter Nielsen , Rolf Berg , Niels Mollegaard
发明人： David Ecker , Ole Buchardt , Michael Egholm , Peter Nielsen , Rolf Berg , Niels Mollegaard
CPC分类号： C12N15/113 , A61K38/00 , B82Y5/00 , C07H21/00 , C07K14/003 , C12N2310/15 , C12N2310/3181 , C12Q1/6813 , C12Q2525/107 , C12Q2537/119
摘要： Peptide nucleic acids and analogues of peptide nucleic acids are used to form duplex, triplex, and other structures with nucleic acids and to modify nucleic acids. The peptide nucleic acids and analogues thereof also are used to modulate protein activity through, for example, transcription arrest, transcription initiation, and site specific cleavage of nucleic acids.
摘要翻译： 肽核酸和肽核酸的类似物用于与核酸形成双链体，三链体和其他结构并修饰核酸。 肽核酸及其类似物也用于通过例如核酸的转录停止，转录起始和位点特异性切割来调节蛋白质活性。
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公开(公告)号：US06713602B1
公开(公告)日：2004-03-30
申请号：US08462977
申请日：1995-06-05
IPC分类号： A61K3800
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , C12Q2535/107
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.
摘要翻译： 称为肽核酸的新一类化合物比相应的DNA更强地结合互补的ssDNA和RNA链。 肽核酸通常包含配体，例如通过合适的接头连接到肽主链上的天然存在的DNA碱基。
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公开(公告)号：US06297016B1
公开(公告)日：2001-10-02
申请号：US09416003
申请日：1999-10-08
申请人： Michael Egholm , Caifu Chen
发明人： Michael Egholm , Caifu Chen
IPC分类号： C12Q168
CPC分类号： C12Q1/6862
摘要： The invention provides methods, kits, and compositions for ligation of PNA-DNA chimeric probes and oligonucleotides when they are hybridized adjacently to template nucleic acids using ligases and ligation reagents. Structural requirements of the chimeras for ligation include 5 to 15 contiguous PNA monomer units, 2 or more contiguous nucleotides, and a 3′ hydroxyl or 5′ hydroxyl terminus. The chimera and/or oligonucleotide may be labelled with fluorescent dyes or other labels. The methods include, for example, oligonucleotide-ligation assays (OLA) and single nucleotide polymorphism detection.
摘要翻译： 当使用连接酶和连接试剂将它们与模板核酸相互杂交时，本发明提供用于连接PNA-DNA嵌合探针和寡核苷酸的方法，试剂盒和组合物。 用于连接的嵌合体的结构要求包括5至15个连续的PNA单体单元，2个或更多个连续核苷酸，以及3'羟基或5'羟基末端。 嵌合体和/或寡核苷酸可以用荧光染料或其他标记物标记。 所述方法包括例如寡核苷酸连接测定（OLA）和单核苷酸多态性检测。
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公开(公告)号：US6107470A
公开(公告)日：2000-08-22
申请号：US225146
申请日：1999-01-04
CPC分类号： C07K14/003
摘要： Peptide nucleic acids containing histidine moieties are provided. These compounds have applications including diagnostics, research and potential therapeutics.
摘要翻译： 提供含有组氨酸部分的肽核酸。 这些化合物具有诊断，研究和潜在治疗的应用。
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