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公开(公告)号：US07256280B2
公开(公告)日：2007-08-14
申请号：US09993808
申请日：2001-11-06
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins that are involved in cell cycle regulation. The invention further provides recombinant expression cassettes, host cells, transgenic plants, and antibody compositions. The present invention provides methods and compositions relating to altering cell cycle protein content, cell cycle progression, cell number and/or composition of plants.
摘要翻译： 本发明提供分离的核酸及其编码的参与细胞周期调节的蛋白质。 本发明还提供了重组表达盒，宿主细胞，转基因植物和抗体组合物。 本发明提供了涉及改变细胞周期蛋白质含量，细胞周期进程，细胞数量和/或植物组成的方法和组合物。
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22.发明授权Isolated LEC1 transcriptional regulator encoding nucleic acids and methods of use thereof 有权编码核酸的分离的LEC1转录调节子及其使用方法公开(公告)号：US07166765B2
公开(公告)日：2007-01-23
申请号：US10744620
申请日：2003-12-22
申请人： Keith S. Lowe , William J. Gordon-Kamm , Theodore M. Klein , Sonriza Rasco-Gaunt , Rebecca E. Cahoon , Xifan Sun , George J. Hoerster , Carolyn Gregory , Ramgopal Nadimpalli , Yumin Tao
发明人： Keith S. Lowe , William J. Gordon-Kamm , Theodore M. Klein , Sonriza Rasco-Gaunt , Rebecca E. Cahoon , Xifan Sun , George J. Hoerster , Carolyn Gregory , Ramgopal Nadimpalli , Yumin Tao
CPC分类号： C07K14/415 , C12N15/8209 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： The invention provides isolated nucleic acids and their encoded proteins which act as transcriptional activators and methods of use thereof. The invention further provides expression cassettes, transformed host cells, transgenic plants and plant parts.
摘要翻译： 本发明提供分离的核酸及其编码的作为转录激活子的蛋白质及其使用方法。 本发明进一步提供表达盒，转化的宿主细胞，转基因植物和植物部分。
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公开(公告)号：US06452070B1
公开(公告)日：2002-09-17
申请号：US09627107
申请日：2000-07-27
申请人： William J. Gordon-Kamm , Keith S. Lowe , Matthew A. Bailey , Carolyn A. Gregory , George J. Hoerster , Brian A. Larkins , Brian R. Dilkes , Ronald Burnett , Young Min Woo
发明人： William J. Gordon-Kamm , Keith S. Lowe , Matthew A. Bailey , Carolyn A. Gregory , George J. Hoerster , Brian A. Larkins , Brian R. Dilkes , Ronald Burnett , Young Min Woo
IPC分类号： A01H100
CPC分类号： C12N15/8209 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： The invention provides novel methods of using viral replicase polypeptides and polynucleotides. Included are methods for increasing transformation frequencies, increasing crop yield, providing a positive growth advantage, modulating cell division, transiently modulating cell division, and for providing a means of positive selection.
摘要翻译： 本发明提供使用病毒复制酶多肽和多核苷酸的新方法。 包括增加转化频率，提高作物产量，提供正增长优势，调节细胞分裂，瞬时调节细胞分裂以及提供正选择的方法的方法。
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公开(公告)号：US06284947B1
公开(公告)日：2001-09-04
申请号：US09257131
申请日：1999-02-25
申请人： William J. Gordon-Kamm , Keith S. Lowe , Matthew A. Bailey , Carolyn A. Gregory , George J. Hoerster , Brian A. Larkins , Brian R. Dilkes , Ronald Burnett , Young Min Woo
发明人： William J. Gordon-Kamm , Keith S. Lowe , Matthew A. Bailey , Carolyn A. Gregory , George J. Hoerster , Brian A. Larkins , Brian R. Dilkes , Ronald Burnett , Young Min Woo
IPC分类号： C12N504
CPC分类号： C12N15/8209 , C12N15/8201 , C12N15/8207 , C12N15/8261 , Y02A40/146
摘要： The invention provides novel methods of using viral replicase polypeptides and polynucleotides. Included are methods for increasing transformation frequencies, increasing crop yield, providing a positive growth advantage, modulating cell division, transiently modulating cell division, and for providing a means of positive selection.
摘要翻译： 本发明提供使用病毒复制酶多肽和多核苷酸的新方法。 包括增加转化频率，提高作物产量，提供正增长优势，调节细胞分裂，瞬时调节细胞分裂以及提供正选择的方法的方法。
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25.发明授权Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof 失效用于生产稳定转化的肥沃单子叶植物及其细胞的方法和组合物
公开(公告)号：US5919675A
公开(公告)日：1999-07-06
申请号：US446933
申请日：1995-05-23
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87 , C12N15/00 , A01H1/06
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to a method for the production of stable, genetically transformed, Zea mays plants that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said plants. The method further comprises transforming said Zea mays plants with a second gene and further still wherein said second gene imparts insect resistance, such as the expression of a Bacillus thuringiensis toxin gene. The method for achieving stable transformation includes tissue culture methods and media, methods for the bombardment of recipient cells with said DNA's, and methods of regenerating fertile plants from transformed cells. The invention also pertains to a method of breeding said transgenic Zea mays plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.
摘要翻译： 本发明涉及一种用于生产稳定的转基因玉米植物的方法，其包含表达草甘膦乙酰转移酶的外源DNA，以赋予所述植物中的次氯氰菊酯抗性。 该方法还包括用第二基因转化所述玉米植物，并且还进一步其中所述第二基因赋予昆虫抗性，例如苏云金芽孢杆菌毒素基因的表达。 实现稳定转化的方法包括组织培养方法和培养基，用所述DNA轰击受体细胞的方法，以及从转化细胞再生可育植物的方法。 本发明还涉及用非转基因植物或转基因植物育种所述转基因玉米植物的方法，以及获得可育转基因植物和种子。
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26.发明授权Methods and compositions for the production of stably transformed fertile monocot plants and cells thereof 失效用于生产稳定转化的可育单子叶植物及其细胞的方法和组合物
公开(公告)号：US5874265A
公开(公告)日：1999-02-23
申请号：US446931
申请日：1995-05-23
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87 , C12N15/00 , A01H1/06
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to a method for the production of stable, genetically transformed cereal plants, other than maize or rice, that comprise an exogenous DNA expressing phosphinothricin acetyl transferase to impart resistance to phosphinothricin in said cereal plants. Cereal plants within the scope of the method include, but are not limited to, wheat, barley and oats. The invention also pertains to a method of breeding said transgenic cereal plants with a either a non-transgenic plant or a transgenic plant, and the obtention of fertile transgenic plants and seed therefrom.
摘要翻译： 本发明涉及一种生产稳定的基因转化谷物植物（玉米或水稻以外）的方法，其包含表达草甘膦乙酰转移酶的外源DNA，以赋予所述谷类植物中的次氯氰菊酯抗性。 在该方法范围内的谷类植物包括但不限于小麦，大麦和燕麦。 本发明还涉及用非转基因植物或转基因植物育种所述转基因谷物植物的方法，以及获得可育转基因植物和种子。
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27.发明授权
公开(公告)号：US5550318A
公开(公告)日：1996-08-27
申请号：US565844
申请日：1990-08-09
申请人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
发明人： Thomas R. Adams , Sheryl A. Chambers , Richard J. Daines , William J. Gordon-Kamm , Albert P. Kausch , Peggy G. Lemaux , Catherine J. Mackey , Mary L. Mangano , James V. O'Brien , Thomas B. Rice , T. Michael Spencer , William G. Start , Nancy G. Willetts
IPC分类号： A01H4/00 , C07K14/415 , C12N5/00 , C12N5/04 , C12N15/05 , C12N15/82 , C12N15/87 , C12N15/00 , A01H1/06
CPC分类号： A01H4/008 , A01H4/00 , A01H4/001 , C07K14/415 , C12N15/8207 , C12N15/87 , C12N5/0025 , C12N5/04 , Y10S47/01
摘要： This invention relates to a reproducible system for the production of stable, genetically transformed maize cells, and to methods of selecting cells that have been transformed. One method of selection disclosed employs the Streptomyces bar gene introduced by microprojectile bombardment into embryogenic maize cells which were grown in suspension cultures, followed by exposure to the herbicide bialaphos. The methods of achieving stable transformation disclosed herein include tissue culture methods and media, methods for the bombardment of recipient cells with the desired transforming DNA, and methods of growing fertile plants from the transformed cells. This invention also relates to the transformed cells and seeds and to the fertile plants grown from the transformed cells and to their pollen.
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公开(公告)号：US08318493B2
公开(公告)日：2012-11-27
申请号：US12756211
申请日：2010-04-08
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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公开(公告)号：US20110159486A1
公开(公告)日：2011-06-30
申请号：US12976531
申请日：2010-12-22
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： Methods and compositions for modulating plant yield are provided. Methods include employing cell cycle switch 52 (ccs52). The ccs52 sequences are used in a variety of methods including modulating plant biomass, growth, or both. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided.
摘要翻译： 提供了调节植物产量的方法和组合物。 方法包括采用细胞周期开关52（ccs52）。 ccs52序列用于多种方法，包括调节植物生物量，生长或两者。 还提供了转化的植物，植物细胞，组织，种子和表达载体。
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公开(公告)号：US20100192263A1
公开(公告)日：2010-07-29
申请号：US12756211
申请日：2010-04-08
CPC分类号： C12N15/8213 , C12N15/102 , C12N15/1051 , C12N15/1093 , C40B40/08
摘要： Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided.
摘要翻译： 提供了使用随机修饰的FRT重组位点群的方法和组合来鉴定，分离和/或表征修饰的FRT重组位点。 重组生成修饰的FRT重组位点可用于多种感兴趣多核苷酸靶向重组方法，包括重组多核苷酸的方法，评估启动子活性，直接选择转化的生物体，最小化或消除由随机整合到基因组中的表达 生物体如植物，去除感兴趣的多核苷酸，组合多个转移盒，反转或切割多核苷酸，以及鉴定和/或表征转录调节区。
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