DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS
    25.
    发明申请
    DETECTION OF NUCLEIC ACID REACTIONS ON BEAD ARRAYS 有权
    检测珠粒阵列上的核酸反应

    公开(公告)号:US20090186349A1

    公开(公告)日:2009-07-23

    申请号:US12212585

    申请日:2008-09-17

    IPC分类号: C12Q1/68

    摘要: The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.

    摘要翻译: 本发明涉及使用微球阵列来检测和定量许多核酸反应的方法和组合物。 本发明用于基因分型,即确定核酸序列,特别是改变,例如核苷酸取代(错配)和单核苷酸多态性(SNP)。 类似地,本发明用于使用多种扩增技术检测和定量核酸靶,包括信号放大和靶扩增两者。 本发明的方法和组合物也可用于核酸测序反应。 所有应用程序可以包括使用适配器序列来允许通用阵列。