公开(公告)号：US5116743A

公开(公告)日：1992-05-26

申请号：US790063

申请日：1991-11-12

申请人： Makoto Goto ,  Terukazu Nara ,  Masato Terasawa ,  Hideaki Yukawa

发明人： Makoto Goto ,  Terukazu Nara ,  Masato Terasawa ,  Hideaki Yukawa

IPC分类号： C12P13/06

CPC分类号： C12P13/06 ,  Y10S435/874 ,  Y10S435/876 ,  Y10S435/877

摘要： There is disclosed a process for producing L-alanine by reacting in a single reaction tank, in an aqueous reaction mixture having a pH of 6 to 10 and containing at least one .alpha.-keto acid, fumaric acid or a salt thereof with ammonia or ammonium ions in the presence of two microorganisms having fumarase inactivity.

公开(公告)号：US09290770B2

公开(公告)日：2016-03-22

申请号：US14238527

申请日：2012-08-21

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N15/77 ,  C12P13/08 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10

CPC分类号： C12N15/77 ,  C12N9/0006 ,  C12N9/0016 ,  C12N9/1022 ,  C12P13/08 ,  C12Y101/01086 ,  C12Y104/01009 ,  C12Y202/01006 ,  Y02P20/52

摘要： A transformant obtainable by introducing one or more of the following DNAs (a), (b), and (c) into a coryneform bacterium as a host.(a) A DNA which encodes acetohydroxy acid synthase derived from Corynebacterium glutamicum and which has a mutation changing the glycine at position 156 to glutamic acid (G156E) in an amino acid sequence encoded by the DNA, or an analog thereof.(b) A DNA which encodes acetohydroxy acid isomeroreductase derived from Corynebacterium glutamicum and which has mutations changing the serine at position 34 to glycine (S34G), the leucine at position 48 to glutamic acid (L48E), and the arginine at position 49 to phenylalanine (R49F) in an amino acid sequence encoded by the DNA, or an analog thereof. (c) A DNA which encodes leucine dehydrogenase derived from Lysinibacillus sphaericus, or an analog thereof.

摘要翻译： 将一种或多种以下DNA（a），（b）和（c）引入作为宿主的棒状细菌获得的转化体。 （a）编码源自谷氨酸棒杆菌的乙酰羟酸合酶的DNA，其在DNA编码的氨基酸序列或其类似物中具有将位置156处的甘氨酸变为谷氨酸（G156E）的突变。 （b）编码源自谷氨酸棒杆菌的乙酰羟酸异构体还原酶的DNA，其具有将第34位的丝氨酸改变为甘氨酸（S34G），将第48位的亮氨酸变为谷氨酸（L48E），将第49位的精氨酸变为苯丙氨酸 （R49F），其由DNA编码的氨基酸序列或其类似物。 （c）编码源自嗜裂杆菌属的亮氨酸脱氢酶的DNA，或其类似物。

公开(公告)号：US08846367B2

公开(公告)日：2014-09-30

申请号：US13988107

申请日：2011-11-17

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N9/90 ,  C12N1/20 ,  C12P19/30 ,  C12P7/22 ,  C12N9/88 ,  C12N15/77

CPC分类号： C12N15/77 ,  C12N9/88 ,  C12P7/22 ,  C12Y401/99002

摘要： Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.

摘要翻译： 提供了通过将编码具有酪氨酸酚裂解酶活性的酶的基因转移到作为宿主的棒状细菌中构建的产生苯酚的转化体。 还提供了一种生产苯酚的方法，其包括在还原条件下使转化体在含有酪氨酸，其盐或其酯的反应混合物中反应的步骤和从反应混合物中收集苯酚的步骤。

公开(公告)号：US20130273624A1

公开(公告)日：2013-10-17

申请号：US13884549

申请日：2011-11-09

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N15/77 ,  C12P7/22

CPC分类号： C12N15/77 ,  C12N9/88 ,  C12P7/22 ,  Y02P20/52

摘要： Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having chorismate-pyruvate lyase activity and a gene which encodes an enzyme having 4-hydroxybenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing a saccharide under reducing conditions, and a step of collecting phenol from the reaction mixture.

摘要翻译： 本发明提供一种生产苯酚的转化体，其通过将编码具有4-硝基苯甲酸脱羧酶活性的酶的基因编码为具有佐罗沙普 - 丙酮酸裂解酶活性的酶的基因转移到作为宿主的棒状细菌中构建。 还提供了一种生产苯酚的方法，其包括使得转化体在还原条件下在含有糖的反应混合物中反应的步骤和从反应混合物中收集苯酚的步骤。

公开(公告)号：US20130267000A1

公开(公告)日：2013-10-10

申请号：US13988107

申请日：2011-11-17

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N15/77 ,  C12P7/22

CPC分类号： C12N15/77 ,  C12N9/88 ,  C12P7/22 ,  C12Y401/99002

摘要： Provided is a phenol-producing transformant constructed by transferring a gene which encodes an enzyme having tyrosine phenol-lyase activity into a coryneform bacterium as a host. Also provided is a process for producing phenol, which comprises a step of allowing the transformant to react in a reaction mixture containing tyrosine, a salt thereof, or an ester thereof under reducing conditions, and a step of collecting phenol from the reaction mixture.

摘要翻译： 提供了通过将编码具有酪氨酸酚裂解酶活性的酶的基因转移到作为宿主的棒状细菌中构建的产生苯酚的转化体。 还提供了一种生产苯酚的方法，其包括在还原条件下使转化体在含有酪氨酸，其盐或其酯的反应混合物中反应的步骤和从反应混合物中收集苯酚的步骤。

公开(公告)号：US08216820B2

公开(公告)日：2012-07-10

申请号：US12988882

申请日：2009-04-15

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12N1/21 ,  C12P7/04

CPC分类号： C12N15/52 ,  C12P7/04 ,  C12R1/15

摘要： A transformant capable of producing isopropanol which is constructed by transferring the following genes (a) to (d) into a coryneform bacterium: (a) an exogenous gene which encodes an enzyme having acetyl-CoA acetyltransferase activity; (b) an exogenous gene which encodes an enzyme having acetoacetyl CoA:acetate CoA-transferase activity; (c) an exogenous gene which encodes an enzyme having acetoacetate decarboxylase activity; and (d) an exogenous gene which encodes an enzyme having isopropanol dehydrogenase activity.

摘要翻译： 能够产生异丙醇的转化体，其通过将下列基因（a）至（d）转移到棒状细菌中构建：（a）编码具有乙酰辅酶A乙酰转移酶活性的酶的外源基因; （b）编码具有乙酰乙酰CoA：乙酸CoA转移酶活性的酶的外源基因; （c）编码具有乙酰乙酸脱羧酶活性的酶的外源基因; 和（d）编码具有异丙醇脱氢酶活性的酶的外源基因。

公开(公告)号：US20120115196A1

公开(公告)日：2012-05-10

申请号：US13258670

申请日：2010-03-29

申请人： Hideaki Yukawa ,  Masayuki Inui

发明人： Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12P7/16 ,  C12N1/21

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/88 ,  Y02E50/10

摘要： A Corynebacterium glutamicum transformant having the capability of producing isobutanol and the following genes (1) to (5): (1) a gene which encodes an enzyme having acetohydroxy acid synthase activity; (2) a gene which encodes an enzyme having acetohydroxy acid isomeroreductase activity; (3) a gene which encodes an enzyme having dihydroxy acid dehydratase activity; (4) a gene which encodes an enzyme having 2-keto acid decarboxylase activity; and (5) a gene which encodes an enzyme having alcohol dehydrogenase activity, at least one of the genes being endogenous, and at least one of the genes being exogenous, efficiently produces isobutanol.

摘要翻译： 具有产生异丁醇能力的谷氨酸棒杆菌转化体和以下基因（1）至（5）：（1）编码具有乙酰羟酸合成酶活性的酶的基因; （2）编码具有乙酰羟酸异构体还原酶活性的酶的基因; （3）编码具有二羟基酸脱水酶活性的酶的基因; （4）编码具有2-酮酸脱羧酶活性的酶的基因; 和（5）编码具有醇脱氢酶活性的酶的基因，至少一个基因是内源的，并且至少一个外源基因有效地产生异丁醇。

公开(公告)号：US20090280548A1

公开(公告)日：2009-11-12

申请号：US12435705

申请日：2009-05-05

申请人： Akihito YOSHIDA ,  Hideaki Yukawa ,  Masayuki Inui

发明人： Akihito YOSHIDA ,  Hideaki Yukawa ,  Masayuki Inui

IPC分类号： C12P3/00 ,  C12M3/00

CPC分类号： C12P3/00 ,  C12M21/04 ,  C12M43/08 ,  C12N9/0006 ,  C12Y101/05006

摘要： In a step of contacting an organic material including formic acid ions and a carbon source other than the formic acid ions with a microorganism having a formate dehydrogenase gene and a hydrogenase gene under an anaerobic condition, concentration of the formic acid ions in the organic material is set to be not less than 0.01 mol/L and not more than 0.5 mol/L, and concentration of the carbon source is set to not less than 0.1 mmol/L and not more than 200 mmol/L. This allows continuously producing hydrogen for a long time, without dropping the ability of the microorganism to produce hydrogen.

摘要翻译： 在含有甲酸离子和除甲酸离子以外的碳源的有机材料与厌氧条件下具有甲酸脱氢酶基因和氢化酶基因的微生物接触的步骤中，有机材料中的甲酸离子的浓度为 设定为0.01mol / L以上且0.5mol / L以下，碳源的浓度为0.1mmol / L以上200mmol / L以下。 这允许长时间连续生产氢气，而不降低微生物产生氢气的能力。

公开(公告)号：US20090170176A1

公开(公告)日：2009-07-02

申请号：US11791731

申请日：2005-12-07

申请人： Hideaki Yukawa ,  Masayuki Inui ,  Akihito Yoshida ,  Naoto Torata

发明人： Hideaki Yukawa ,  Masayuki Inui ,  Akihito Yoshida ,  Naoto Torata

IPC分类号： C12P3/00 ,  C12N1/21

CPC分类号： C12N9/0095 ,  C12N9/0008 ,  C12P3/00 ,  C12Y112/07002

摘要： The present invention provides a microorganism which possesses the formate dehydrogenase gene and hydrogenase gene and contains an exogenous transcription activator gene for formate hydrogen lyase system, characterized in that said microorganism shows the transcription activator for formate hydrogen lyase system highly expressed therein and shows an improved function to generate hydrogen from formic acid, and a process for producing hydrogen using the microorganism.Utilization of the microorganism of the present invention enables the hydrogen production from an organic substrate to be accomplished on a practical, commercial scale. The hydrogen to be produced by the present invention, which is free of carbon monoxide being causative to poisoning of the electrode catalyst for fuel cells, is useful as a fuel for fuel cells.

摘要翻译： 本发明提供一种具有甲酸脱氢酶基因和氢化酶基因并含有甲酸氢裂解酶系统的外源转录激活子基因的微生物，其特征在于所述微生物显示出在其中高度表达的甲酸氢裂解酶系统的转录激活剂，并显示出改善的功能 从甲酸产生氢气，以及使用微生物生产氢气的方法。 本发明的微生物的利用使得能够以实用的商业规模实现有机底物的氢生产。 本发明生产的不含作为燃料电池用电极催化剂中毒的一氧化碳的氢气可用作燃料电池用燃料。

公开(公告)号：US20070087423A1

公开(公告)日：2007-04-19

申请号：US10566129

申请日：2004-07-28

申请人： Shikiko Murakami ,  Kaori Nakata ,  Shohei Okino ,  Yuko Ikenaga ,  Masayuki Inui ,  Hideaki Yukawa

发明人： Shikiko Murakami ,  Kaori Nakata ,  Shohei Okino ,  Yuko Ikenaga ,  Masayuki Inui ,  Hideaki Yukawa

IPC分类号： C12P7/46 ,  C12N1/21

CPC分类号： C12R1/15 ,  C12P7/46

摘要： The present invention provides an aerobic coryneform bacterium transformant in which a lactate dehydrogenase gene is disrupted, and a pyruvate carboxylase gene is recombined so as to be highly expressed by a genetic engineering method. The aerobic coryneform bacterium transformant of the present invention can produce dicarboxylic acids from saccharides at a high production rate.

摘要翻译： 本发明提供一种有氧棒状细菌转化体，其中乳酸脱氢酶基因被破坏，并且丙酮酸羧化酶基因被重组以通过遗传工程方法高度表达。 本发明的有氧棒状细菌转化体可以高生产率从糖生产二羧酸。