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公开(公告)号：US20090123984A1
公开(公告)日：2009-05-14
申请号：US11911660
申请日：2006-04-18
申请人： Joe Chappell , Shuiqin Wu , Michel Schalk , Athony Clark
发明人： Joe Chappell , Shuiqin Wu , Michel Schalk , Athony Clark
CPC分类号： C12N9/1085 , C12N9/88 , C12N15/8243
摘要： The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.
摘要翻译： 本发明涉及具有改变的萜烯含量的转化植物，优选地过度积聚单或三萜烯。 通过用编码萜烯合成酶（TS）和异戊烯基转移酶（PRT）的基因转化植物，获得了累积至少1000ng / g特定萜烯新鲜叶的植物。 本发明提供了一种用于生产萜烯的有利系统，因为可以在植物中生产本领域技术人员可选择的任何所需的单或三萜烯。 优选地，转化的植物含有至少一种靶向TS和PRT的重组质体。
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公开(公告)号：US20060206957A1
公开(公告)日：2006-09-14
申请号：US11440105
申请日：2006-05-23
申请人： Michel Schalk
发明人： Michel Schalk
摘要： The invention relates to sesquiterpene synthases from Patchouli plants (Pogostemon cablin), and methods of their production and use. In one embodiment, the invention provides nucleic acids comprising a nucleotide sequence as described herein that encodes for at least one sesquiterpene synthase. In a further embodiment, the invention also provides for sesquiterpene synthases and methods of making and using these enzymes. For example, sesquiterpene synthases of the invention may be used to convert farnesyl-pyrophosphate to various sesquiterpenes including patchoulol, γ-curcumene and other germacrane-type sesquiterpenes.
摘要翻译： 本发明涉及广藿香植物（Pogostemon cablin）的倍半萜合酶及其生产和使用方法。 在一个实施方案中，本发明提供了包含编码至少一种倍半萜合酶的如本文所述的核苷酸序列的核酸。 在另一个实施方案中，本发明还提供倍半萜合酶和制备和使用这些酶的方法。 例如，本发明的倍半萜烯合成酶可用于将焦亚磷酸法呢酯转化为各种倍半萜烯，包括patchoulol，γ-姜黄素和其他锗酸型倍半萜烯。
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公开(公告)号：US20130102038A1
公开(公告)日：2013-04-25
申请号：US13695840
申请日：2011-05-05
申请人： Michel Schalk , Fabienne Deguerry
发明人： Michel Schalk , Fabienne Deguerry
CPC分类号： C12P15/00 , C12N9/88 , C12N15/8243 , C12P5/002 , C12Y402/0307
摘要： A method of producing patchoulol and 7-epi-α-selinene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). The method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery.
摘要翻译： 通过使至少一种多肽与法呢基磷酸（fpp）接触来生产patclolol和7-表 - α-塞来林的方法。 该方法可以在体外或体内进行，以产生可用于香料领域的化妆品patchoulol和7-表阿尔赛尔烯。
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公开(公告)号：US20120021475A1
公开(公告)日：2012-01-26
申请号：US13258193
申请日：2010-05-12
申请人： Michel Schalk , Fabienne Deguerry
发明人： Michel Schalk , Fabienne Deguerry
摘要： A method of producing (+)-zizaene by contacting at least one polypeptide with farnesyl pyrophosphate (FPP) in vitro or in vivo to produce (+)-zizaene, a compound which can be used as precursor for diverse compounds useful in the fields of perfumery and flavoring. An amino acid sequence of a polypeptide useful in the method, a nucleic acid encoding the polypeptide of the invention, an expression vector containing the nucleic acid and a non-human host organism or a cell transformed to be used in the method of producing (+)-zizaene are also disclosed.
摘要翻译： 通过在体外或体内使至少一种多肽与法呢基焦磷酸（FPP）接触以制备（+） - izaene的方法，以产生（+） - iza，一种可用作多种化合物的前体的化合物，其可用于以下领域： 香料和香料。 可用于该方法的多肽的氨基酸序列，编码本发明多肽的核酸，含有核酸和非人宿主生物的转录的转化细胞（+ ）-zizaene也被公开。
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公开(公告)号：US08017835B2
公开(公告)日：2011-09-13
申请号：US11911660
申请日：2006-04-18
申请人： Joe Chappell , Shuiqin Wu , Michel Schalk , Athony Clark
发明人： Joe Chappell , Shuiqin Wu , Michel Schalk , Athony Clark
CPC分类号： C12N9/1085 , C12N9/88 , C12N15/8243
摘要： The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.
摘要翻译： 本发明涉及具有改变的萜烯含量的转化植物，优选地过度积聚单或三萜烯。 通过用编码萜烯合成酶（TS）和异戊烯基转移酶（PRT）的基因转化植物，获得了累积至少1000ng / g特定萜烯新鲜叶的植物。 本发明提供了一种用于生产萜烯的有利系统，因为可以在植物中生产本领域技术人员可选择的任何所需的单或三萜烯。 优选地，转化的植物含有至少一种靶向TS和PRT的重组质体。
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公开(公告)号：US20090205060A1
公开(公告)日：2009-08-13
申请号：US12366573
申请日：2009-02-05
申请人： Michel Schalk , Fabienne Deguerry
发明人： Michel Schalk , Fabienne Deguerry
IPC分类号： A01K67/027 , C07H21/04 , C12N5/10 , C12N15/63 , C12P21/02 , C12P7/02 , C12Q1/68 , C12N9/00 , C12P5/00
摘要： The invention relates to sesquiterpene synthases from Patchouli plants (Pogostemon cablin), and methods of their production and use. In one embodiment, the invention provides nucleic acids comprising a nucleotide sequence as described herein that encodes for at least one sesquiterpene synthase. In a further embodiment, the invention also provides for sesquiterpene synthases and methods of making and using these enzymes. For example, sesquiterpene synthases of the invention may be used to convert farnesyl-pyrophosphate to various sesquiterpenes including patchoulol, γ-curcumene and other germacrane-type sesquiterpenes.
摘要翻译： 本发明涉及广藿香植物（Pogostemon cablin）的倍半萜合酶及其生产和使用方法。 在一个实施方案中，本发明提供了包含编码至少一种倍半萜合酶的如本文所述的核苷酸序列的核酸。 在另一个实施方案中，本发明还提供倍半萜合酶和制备和使用这些酶的方法。 例如，本发明的倍半萜烯合成酶可用于将焦亚磷酸法呢酯转化为各种倍半萜烯，包括patchoulol，γ-姜黄素和其他锗酸型倍半萜烯。
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公开(公告)号：US20080268500A1
公开(公告)日：2008-10-30
申请号：US11914536
申请日：2006-06-08
申请人： Michel Schalk
发明人： Michel Schalk
IPC分类号： C12P21/04 , C12N15/11 , C12N9/00 , C12N15/00 , A01H5/00 , C12N5/04 , C12N1/14 , C12N1/20 , C12P7/64
摘要： The present invention relates to novel terpene synthases. The terpene synthases are capable of synthesising mono-, bi- and/or tri-cyclic sesquiterpenes having a C2-C7 or a C3-C7 bond, starting from an acyclic pyrophosphate terpene precursor, farnesyl-pyrophosphate. Accordingly, for the first time, sesquiterpene synthases catalyzing the cyclisation to the santalene and bergamotene carbon skeleton are disclosed. The present invention further relates to nucleic acid sequences encoding the sesquiterpene synthases and to methods for making terpenoids.
摘要翻译： 本发明涉及新颖的萜烯合成酶。 萜烯合酶能够合成具有C2-C7或C3-C7键的单环，双环和/或三环倍半萜烯，从非环状焦磷酸萜烯前体法尼基焦磷酸酯开始。 因此，首次公开了催化环化为呫吨烯和香叶碳骨架的倍半萜烯合成酶。 本发明还涉及编码倍半萜合酶的核酸序列和制备萜类化合物的方法。
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公开(公告)号：US08927238B2
公开(公告)日：2015-01-06
申请号：US13695840
申请日：2011-05-15
申请人： Michel Schalk , Fabienne Deguerry
发明人： Michel Schalk , Fabienne Deguerry
CPC分类号： C12P15/00 , C12N9/88 , C12N15/8243 , C12P5/002 , C12Y402/0307
摘要： A method of producing patchoulol and 7-epi-α-selinene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). The method may be carried out in vitro or in vivo to produce patchoulol and 7-epi-α-selinene, compounds which can be useful in the field of perfumery.
摘要翻译： 通过使至少一种多肽与法呢基磷酸（fpp）接触来生产帕替洛尔和7-表 - α-塞来林的方法。 该方法可以在体外或体内进行，以产生可用于香料领域的化合物，其可用于香草醇和7-表 - α-硒烯。
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公开(公告)号：US08877461B2
公开(公告)日：2014-11-04
申请号：US13124866
申请日：2009-12-09
申请人： Michel Schalk
发明人： Michel Schalk
IPC分类号： C12P21/06
CPC分类号： C12P5/002 , C12N9/88 , C12P5/007 , C12Y402/03083
摘要： A method of producing β-santalene by contacting at least one polypeptide with farnesyl pyrophosphate (FPP). This method may be carried out in vitro or in vivo to produce β-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing the nucleic acid are also disclosed, along with a non-human host organism or a cell transformed to be used in the method of producing β-santalene.
摘要翻译： 通过使至少一种多肽与法呢基焦磷酸酯（FPP）接触来生产β-花生四烯的方法。 该方法可以在体外或体内进行以产生在香料和香料领域中非常有用的化合物。 本发明还提供了可用于本发明方法的多肽的氨基酸序列。 还公开了编码本发明多肽的核酸和含有该核酸的表达载体，以及非人宿主生物体或转化用于生产β-花生四烯的方法的细胞。
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公开(公告)号：US08703454B2
公开(公告)日：2014-04-22
申请号：US13258193
申请日：2010-05-12
申请人： Michel Schalk , Fabienne Deguerry
发明人： Michel Schalk , Fabienne Deguerry
摘要： A method of producing (+)-zizaene by contacting at least one polypeptide with farnesyl pyrophosphate (FPP) in vitro or in vivo to produce (+)-zizaene, a compound which can be used as precursor for diverse compounds useful in the fields of perfumery and flavoring. An amino acid sequence of a polypeptide useful in the method, a nucleic acid encoding the polypeptide of the invention, an expression vector containing the nucleic acid and a non-human host organism or a cell transformed to be used in the method of producing (+)-zizaene are also disclosed.
摘要翻译： 通过在体外或体内使至少一种多肽与法呢基焦磷酸（FPP）接触以制备（+） - izaene的方法，以产生（+） - iza，一种可用作多种化合物的前体的化合物，其可用于以下领域： 香料和香料。 可用于该方法的多肽的氨基酸序列，编码本发明多肽的核酸，含有核酸和非人宿主生物的转录的转化细胞（+ ）-zizaene也被公开。
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