公开(公告)号：US20090123984A1

公开(公告)日：2009-05-14

申请号：US11911660

申请日：2006-04-18

申请人： Joe Chappell ,  Shuiqin Wu ,  Michel Schalk ,  Athony Clark

发明人： Joe Chappell ,  Shuiqin Wu ,  Michel Schalk ,  Athony Clark

IPC分类号： C12P5/00 ,  A01H5/00 ,  C12N5/10 ,  C12N15/82

CPC分类号： C12N9/1085 ,  C12N9/88 ,  C12N15/8243

摘要： The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.

摘要翻译： 本发明涉及具有改变的萜烯含量的转化植物，优选地过度积聚单或三萜烯。 通过用编码萜烯合成酶（TS）和异戊烯基转移酶（PRT）的基因转化植物，获得了累积至少1000ng / g特定萜烯新鲜叶的植物。 本发明提供了一种用于生产萜烯的有利系统，因为可以在植物中生产本领域技术人员可选择的任何所需的单或三萜烯。 优选地，转化的植物含有至少一种靶向TS和PRT的重组质体。

公开(公告)号：US20080201796A1

公开(公告)日：2008-08-21

申请号：US11916272

申请日：2006-06-26

申请人： Joe Chappell ,  Shuiqin Wu ,  Michel Schalk ,  Athony Clark

发明人： Joe Chappell ,  Shuiqin Wu ,  Michel Schalk ,  Athony Clark

IPC分类号： A01H1/00 ,  A01H5/00 ,  C12N15/82 ,  C12N15/00

CPC分类号： C12N15/8243 ,  C12N9/0006 ,  C12N9/1085

摘要： The present invention relates to plant expressing transgenes and to plants transformed to comprise additional copies of genes, said genes encoding at least a HMGR-CoA reductase and a terpene synthase. The invention further claims methods for preparing the plants, and a method for producing terpenes. The present thus provides a reliable and cost effective platform for generating any terpene, in particular any mono- and/or sesquiterpene of interest. For example, the skilled person may use any gene encoding a sesquiterpene synthase for accumulating the respective sesquiterpene in the plant of the present invention.

摘要翻译： 本发明涉及表达转基因的植物和转化成包含编码另外的基因拷贝的植物，所述基因编码至少HMGR-CoA还原酶和萜烯合酶。 本发明进一步要求制备植物的方法以及萜烯的制备方法。 因此，本发明提供了用于产生任何萜烯，特别是感兴趣的任何单 - 和/或倍半萜的可靠和具有成本效益的平台。 例如，技术人员可以使用编码倍半萜合酶的任何基因来在本发明的植物中积累相应的倍半萜烯。

公开(公告)号：US08017835B2

公开(公告)日：2011-09-13

申请号：US11911660

申请日：2006-04-18

申请人： Joe Chappell ,  Shuiqin Wu ,  Michel Schalk ,  Athony Clark

发明人： Joe Chappell ,  Shuiqin Wu ,  Michel Schalk ,  Athony Clark

IPC分类号： C12N15/29 ,  C12N15/31 ,  C12N15/52 ,  C12N15/82

CPC分类号： C12N9/1085 ,  C12N9/88 ,  C12N15/8243

摘要： The present invention relates to transformed plants with an altered terpene content, preferably over-accumulating a mono- or sesqui-terpene. By transformation of plants with genes encoding terpene synthases (TS), and prenyl transferases (PRT), plants accumulating at least 1000 ng/per g of fresh leaf of a specific terpene were obtained. The present invention provides an advantageous system for production of terpenes in that any desired mono- or sesqui-terpene at the choice of the skilled person can be produced in plants. Preferably, the transformed plants contain at least one recombinant plastid targeted TS and PRT.

摘要翻译： 本发明涉及具有改变的萜烯含量的转化植物，优选地过度积聚单或三萜烯。 通过用编码萜烯合成酶（TS）和异戊烯基转移酶（PRT）的基因转化植物，获得了累积至少1000ng / g特定萜烯新鲜叶的植物。 本发明提供了一种用于生产萜烯的有利系统，因为可以在植物中生产本领域技术人员可选择的任何所需的单或三萜烯。 优选地，转化的植物含有至少一种靶向TS和PRT的重组质体。

公开(公告)号：US09297004B2

公开(公告)日：2016-03-29

申请号：US12918140

申请日：2009-03-04

申请人： Michel Schalk

发明人： Michel Schalk

IPC分类号： C12N15/29 ,  C12N15/60 ,  C12N15/82 ,  C12P15/00 ,  C12N9/88 ,  C12P5/00

CPC分类号： C12N9/88 ,  C12P5/007 ,  C12P15/00 ,  C12Y402/03082

摘要： The present invention provides a method of producing α-santalene by contacting at least one polypeptide with farnesyl phyrophosphate (fpp). In particular, the method may be carried out in vitro or in vivo to produce α-santalene, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of a polypeptide useful in the method of the invention. A nucleic acid encoding the polypeptide of the invention and an expression vector containing the nucleic acid represent part of the present invention. A non-human host organism and a cell transformed to be used in the method of producing α-santalene are also part of the present invention.

摘要翻译： 本发明提供了通过使至少一种多肽与法呢基磷酸（fpp）接触来生产α-檀香草的方法。 特别地，该方法可以在体外或体内进行以产生α-香豆素，在香料和香料领域中非常有用的化合物。 本发明还提供了可用于本发明方法的多肽的氨基酸序列。 编码本发明多肽的核酸和含有核酸的表达载体代表本发明的一部分。 非人宿主生物体和转化用于生产α-檀香烃的方法的细胞也是本发明的一部分。

公开(公告)号：US08048658B2

公开(公告)日：2011-11-01

申请号：US12279300

申请日：2007-02-13

申请人： Anthony Clark ,  Jérôme Maury ,  Mohammad Ali Asadollahi ,  Kasper Møller ,  Jens Nielsen ,  Michel Schalk

发明人： Anthony Clark ,  Jérôme Maury ,  Mohammad Ali Asadollahi ,  Kasper Møller ,  Jens Nielsen ,  Michel Schalk

IPC分类号： C12P5/00 ,  C12N1/19 ,  C12N1/63 ,  C07C403/00

CPC分类号： C12N15/52 ,  C12P23/00

摘要： The present invention relates to a microorganism capable of producing a terpene of choice. The microorganism expresses a heterologous pathway for the formation of isoprene units and, preferably, a heterologous terpene synthase. In this way, high amounts of terpene can be isolated from the medium of the microorganism.

摘要翻译： 本发明涉及能够产生选择的萜烯的微生物。 微生物表达用于形成异戊二烯单元的异源途径，优选异源萜烯合酶。 以这种方式，可以从微生物的培养基中分离出大量的萜烯。

公开(公告)号：US20110041218A1

公开(公告)日：2011-02-17

申请号：US12867861

申请日：2009-02-12

申请人： Michel Schalk

发明人： Michel Schalk

IPC分类号： C12P7/02 ,  C12N9/16 ,  C07H21/00 ,  C12N15/63 ,  C12N5/10 ,  C12N1/21 ,  C12N1/19 ,  A01H5/00

CPC分类号： C12P7/18 ,  C07D307/92 ,  C07D311/92 ,  C07K2319/00 ,  C12N9/88

摘要： The present invention provides a method of producing sclareol, said method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, said method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing said nucleic acid, as well as a non-human host organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.

摘要翻译： 本发明提供了生产香紫苏醇的方法，所述方法包括将具有香紫苏醇合成酶活性的特定多肽与二硫代二磷酸酯（LPP）接触。 特别地，所述方法可以在体外或体内进行以产生香紫苏醇，香料和香料领域中非常有用的化合物。 本发明还提供了该方法中使用的多肽的氨基酸序列。 衍生自丹参的核酸并编码本发明的多肽，含有所述核酸的表达载体以及非人宿主生物或转化成携带相同核酸的细胞也是本发明的一部分 。

公开(公告)号：US06787337B1

公开(公告)日：2004-09-07

申请号：US09713794

申请日：2000-11-15

申请人： Yannick Batard ,  Francis Durst ,  Michel Schalk ,  Daniele Werck-Reichhart

发明人： Yannick Batard ,  Francis Durst ,  Michel Schalk ,  Daniele Werck-Reichhart

IPC分类号： C12P2100

CPC分类号： C12N9/0042 ,  C12N15/67 ,  C12N15/81

摘要： The present invention relates to a recombinant non-yeast DNA, which encodes a protein of interest, wherein an unmodified DNA corresponding to the recombinant non-yeast DNA contains a region having a high content of codons that are poorly suited to yeasts, wherein a number of the codons that are poorly suited to yeasts are replaced in said region of the recombinant non-yeast DNA with synonymous codons coding for the same amino acid that are well-suited to yeasts, and wherein the number of replaced codons is sufficient to permit expression in yeasts. The present invention also relates to DNA sequences which originate from dicotyledonous or monocotyledonous plants, and in particular plants of the graminae family which are selected from among wheat, barley, oats, rice, maize, sorghum and cane sugar, as well as to vectors and transformed yeasts which contain the DNA sequences of the invention.

摘要翻译： 本发明涉及编码感兴趣的蛋白质的重组非酵母DNA，其中对应于重组非酵母DNA的未修饰的DNA含有具有高度不适于酵母的密码子的高含量区域，其中数目 的不适于酵母的密码子在所述重组非酵母DNA的所述区域中被替换，其具有编码相当于酵母的相同氨基酸的同义密码子，并且其中替换的密码子的数目足以允许表达 在酵母中 本发明还涉及源自双子叶植物或单子叶植物的DNA序列，特别是选自小麦，大麦，燕麦，稻，玉米，高粱和蔗糖的格氏族的植物，以及载体和 转化酵母含有本发明的DNA序列。

公开(公告)号：US20150218588A1

公开(公告)日：2015-08-06

申请号：US14355840

申请日：2012-10-25

申请人： Michel Schalk ,  Fabienne Deguerry

发明人： Michel Schalk ,  Fabienne Deguerry

IPC分类号： C12P5/00 ,  C12N9/02 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40

CPC分类号： C12P5/007 ,  C12N9/0071 ,  C12P7/02 ,  C12P7/24 ,  C12P7/40

摘要： The present invention provides the nucleic acid and the amino acid sequences of a cytochrome P450 capable of oxidizing terpene molecules. It also provides a method of oxidizing terpene molecules comprising contacting the cytochrome P450 of the invention with the terpene molecule intended to be oxidized. In particular, said method may be carried out in vitro or in vivo to produce oxidized terpene molecules, which may be used in different technical fields such as for example perfumery and flavoring. The present invention also provides an expression vector containing the nucleic acid. A non-human host organism or a cell transformed with the nucleic acid is also an object of the invention.

摘要翻译： 本发明提供能够氧化萜烯分子的细胞色素P450的核酸和氨基酸序列。 它还提供氧化萜烯分子的方法，包括将本发明的细胞色素P450与预期被氧化的萜烯分子接触。 特别地，所述方法可以在体外或体内进行以产生氧化萜烯分子，其可用于不同的技术领域，例如香料和调味剂。 本发明还提供含有该核酸的表达载体。 非人宿主生物体或用该核酸转化的细胞也是本发明的目的。

公开(公告)号：US08058046B2

公开(公告)日：2011-11-15

申请号：US12366573

申请日：2009-02-05

申请人： Michel Schalk ,  Fabienne Deguerry

发明人： Michel Schalk ,  Fabienne Deguerry

IPC分类号： C12N9/10

CPC分类号： C12P7/22 ,  C12N9/88 ,  C12P5/007 ,  C12P15/00

摘要： The invention relates to sesquiterpene synthases from Patchouli plants (Pogostemon cablin), and methods of their production and use. In one embodiment, the invention provides nucleic acids comprising a nucleotide sequence as described herein that encodes for at least one sesquiterpene synthase. In a further embodiment, the invention also provides for sesquiterpene synthases and methods of making and using these enzymes. For example, sesquiterpene synthases of the invention may be used to convert farnesyl-pyrophosphate to various sesquiterpenes including patchoulol, γ-curcumene and other germacrane-type sesquiterpenes.

摘要翻译： 本发明涉及广藿香植物（Pogostemon cablin）的倍半萜合酶及其生产和使用方法。 在一个实施方案中，本发明提供了包含编码至少一种倍半萜合酶的如本文所述的核苷酸序列的核酸。 在另一个实施方案中，本发明还提供倍半萜合酶和制备和使用这些酶的方法。 例如，本发明的倍半萜烯合酶可用于将焦亚磷酸法呢酯转化为各种倍半萜，包括帕丘洛尔，γ-姜黄等多种锗类倍半萜烯。

公开(公告)号：US20100311134A1

公开(公告)日：2010-12-09

申请号：US12865298

申请日：2008-01-29

申请人： Michel Schalk

发明人： Michel Schalk

IPC分类号： C12P7/22 ,  C12N9/00 ,  C07H21/04 ,  C12N15/63 ,  A01H5/00 ,  C12N1/00 ,  C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12N5/10 ,  C12N1/15

CPC分类号： C12P7/02 ,  C12N9/16 ,  C12P7/18

摘要： The present invention provides a method of producing sclareol, said method comprising contacting a particular polypeptide having a sclareol synthase activity with labdenediol diphosphate (LPP). In particular, said method may be carried out in vitro or in vivo to produce sclareol, a very useful compound in the fields of perfumery and flavoring. The present invention also provides the amino acid sequence of the polypeptide used in the method. A nucleic acid derived from Salvia sclarea and encoding the polypeptide of the invention, an expression vector containing said nucleic acid, as well as a non-human organism or a cell transformed to harbor the same nucleic acid, are also part of the present invention.

摘要翻译： 本发明提供了生产香紫苏醇的方法，所述方法包括将具有香紫苏醇合成酶活性的特定多肽与二硫代二磷酸酯（LPP）接触。 特别地，所述方法可以在体外或体内进行以产生香紫苏醇，香料和香料领域中非常有用的化合物。 本发明还提供了该方法中使用的多肽的氨基酸序列。 编码本发明多肽的编码本发明多肽的核酸，含有所述核酸的表达载体以及转化成携带相同核酸的非人生物体或细胞也是本发明的一部分。