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21.发明申请INFORMATION ACQUISITION METHOD, INFORMATION ACQUISITION APPARATUS AND DISEASE DIAGNOSIS METHOD 有权信息获取方法，信息获取装置和疾病诊断方法公开(公告)号：US20100227308A1
公开(公告)日：2010-09-09
申请号：US12712131
申请日：2010-02-24
申请人： Hiroyuki Hashimoto , Katsuaki Kuge , Manabu Komatsu , Kumi Nakamura , Kazuhiro Ban , Takeshi Imamura , Shin Kobayashi , Tadashi Okamoto
发明人： Hiroyuki Hashimoto , Katsuaki Kuge , Manabu Komatsu , Kumi Nakamura , Kazuhiro Ban , Takeshi Imamura , Shin Kobayashi , Tadashi Okamoto
CPC分类号： G01N33/6848 , G01N33/4833
摘要： An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry.
摘要翻译： 一种用于获取关于目标对象的信息的信息获取方法，其包括使用促进所述目标对象的电离的物质促进所述目标对象的电离以使所述目标对象发射的步骤以及获取所述质量信息的步骤 的飞行目标物体使用飞行时间二次离子质谱法。
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22.发明授权Information acquisition method, information acquisition apparatus and disease diagnosis method 失效信息采集方法，信息获取装置及疾病诊断方法公开(公告)号：US07701138B2
公开(公告)日：2010-04-20
申请号：US11319233
申请日：2005-12-28
申请人： Hiroyuki Hashimoto , Katsuaki Kuge , Manabu Komatsu , Kumi Nakamura , Kazuhiro Ban , Takeshi Imamura , Shin Kobayashi , Tadashi Okamoto
发明人： Hiroyuki Hashimoto , Katsuaki Kuge , Manabu Komatsu , Kumi Nakamura , Kazuhiro Ban , Takeshi Imamura , Shin Kobayashi , Tadashi Okamoto
IPC分类号： H01J17/26
CPC分类号： G01N33/6848 , G01N33/4833
摘要： An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry.
摘要翻译： 一种用于获取关于目标对象的信息的信息获取方法，其包括使用促进所述目标对象的电离的物质促进所述目标对象的电离以使所述目标对象发射的步骤以及获取所述质量信息的步骤 的飞行目标物体使用飞行时间二次离子质谱法。
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23.发明申请公开(公告)号：US20060183235A1
公开(公告)日：2006-08-17
申请号：US11319233
申请日：2005-12-28
申请人： Hiroyuki Hashimoto , Katsuaki Kuge , Manabu Komatsu , Kumi Nakamura , Kazuhiro Ban , Takeshi Imamura , Shin Kobayashi , Tadashi Okamoto
发明人： Hiroyuki Hashimoto , Katsuaki Kuge , Manabu Komatsu , Kumi Nakamura , Kazuhiro Ban , Takeshi Imamura , Shin Kobayashi , Tadashi Okamoto
IPC分类号： G01N33/00
CPC分类号： G01N33/6848 , G01N33/4833
摘要： An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry.
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公开(公告)号：US09448241B2
公开(公告)日：2016-09-20
申请号：US13576124
申请日：2011-03-28
申请人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
发明人： Jumpei Naito , Shuya Satoh , Manabu Komatsu , Hiroyuki Hashimoto
CPC分类号： G01N33/6851 , H01J49/00 , Y10T436/182
摘要： The problem to be solved by the present invention is to provide a method for treating a polypeptide including a cysteine residue for enhancing the sensitivity of detection thereof in mass spectrometry using charged particles. A mass spectrometry method for a polypeptide including a cysteine residue is provided which includes treating the polypeptide including a cysteine residue with a cyanation agent, treating the cyanated polypeptide with a base, and then desorption ionizing the base-treated polypeptides using charged particles for mass spectrometry.
摘要翻译： 本发明要解决的问题是提供一种用于在使用带电粒子的质谱法中治疗包含半胱氨酸残基的多肽以提高其检测灵敏度的方法。 提供了包括半胱氨酸残基的多肽的质谱法，其包括用氰化剂处理包含半胱氨酸残基的多肽，用碱处理氰化多肽，然后使用带电粒子对基质处理的多肽进行解吸，用于质谱法 。
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公开(公告)号：US08754363B2
公开(公告)日：2014-06-17
申请号：US13575600
申请日：2011-01-31
申请人： Koichi Tanji , Manabu Komatsu , Hiroyuki Hashimoto
发明人： Koichi Tanji , Manabu Komatsu , Hiroyuki Hashimoto
CPC分类号： H01J49/0036 , H01J49/0004
摘要： A more effective noise reduction method is provided. In the method, when mass spectrum information having a spatial distribution is processed, the whole data is taken as three-dimensional data (positional information is stored in an xy plane, and spectral information is stored along a z-axis direction), and three-dimensional wavelet noise reduction is performed by applying preferable basis functions to a spectral direction and a peak distribution direction (in-plane direction).
摘要翻译： 提供了更有效的降噪方法。 在该方法中，当处理具有空间分布的质谱信息时，将整个数据作为三维数据（位置信息存储在xy平面中，并且光谱信息沿z轴方向存储），并且三个 通过对光谱方向和峰分布方向（面内方向）应用优选的基函数来进行维小波噪声降低。
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26.发明授权Information acquiring apparatus and information acquiring method for acquiring mass-related information 失效信息获取装置和用于获取大量相关信息的信息获取方法公开(公告)号：US08704194B2
公开(公告)日：2014-04-22
申请号：US13083510
申请日：2011-04-08
申请人： Manabu Komatsu , Hiroyuki Hashimoto
发明人： Manabu Komatsu , Hiroyuki Hashimoto
CPC分类号： H01J49/06 , H01J49/0463
摘要： Target molecules in a sample can be detected at an improved sensitivity by means of a mass spectrometer. A sample with or without a matrix is placed on a substrate and irradiated with a converged and pulsed primary beam selected from an ion beam, a neutral particle beam or a laser beam. Secondary ions and neutral molecules are emitted along with protons from the irradiated point of the sample as an electric field is applied between the substrate and an extraction electrode disposed above the substrate. A proton-control electrode is arranged in axial symmetry with the trajectory of the primary beam. A voltage is applied thereto so that the generated electric field decelerates the flying protons to raise their adhering efficiency to the flying neutral molecules.
摘要翻译： 可以通过质谱仪以更高的灵敏度检测样品中的靶分子。 将具有或不具有矩阵的样品放置在基底上并用从离子束，中性粒子束或激光束中选择的会聚且脉冲的一次束照射。 随着电场施加在基板和设置在基板之上的引出电极之间，二次离子和中性分子与质子的辐射点一起被发射出来。 质子控制电极与主光束的轨迹轴对称。 施加电压使得所产生的电场使飞行质子减速，以提高它们对飞行中性分子的附着效率。
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27.发明授权Treatment solution, method for pretreatment, method for acquiring information and method for detection 有权治疗方案，预处理方法，获取信息的方法和检测方法公开(公告)号：US08084214B2
公开(公告)日：2011-12-27
申请号：US12098822
申请日：2008-04-07
IPC分类号： G01N33/53
CPC分类号： G01N33/6848 , B82Y15/00 , G01N33/54313
摘要： The present invention intends to provide a high sensitivity method for identifying the type of a protein contained in each cell with a diameter of ten to several tens μm and a sample treatment solution required for it. The above is achieved by three steps of (1) treating a slice of a diseased tissue with an aqueous solution containing gold particles and a digestive enzyme, and digesting restrictively a protein of interest, (2) measuring a 2-dimensional distribution of fragmented peptides by TOF-SIMS, and (3) visualizing a 2-dimensional distribution of the target protein in the slice of the diseased tissue using the results of the proteome analysis and by means of a numerical analysis.
摘要翻译： 本发明旨在提供用于鉴定直径为10至几十μm的每个电池中所含的蛋白质的类型的高灵敏度方法以及所需的样品处理溶液。 上述通过以下三个步骤实现：（1）用含有金颗粒和消化酶的水溶液处理患病组织的切片，并限制性地消化目的蛋白质，（2）测量片段肽的2维分布 通过TOF-SIMS，以及（3）使用蛋白质组分析的结果并通过数值分析来显现病变组织切片中靶蛋白的二维分布。
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28.发明申请公开(公告)号：US20110248156A1
公开(公告)日：2011-10-13
申请号：US13083510
申请日：2011-04-08
申请人： Manabu Komatsu , Hiroyuki Hashimoto
发明人： Manabu Komatsu , Hiroyuki Hashimoto
CPC分类号： H01J49/06 , H01J49/0463
摘要： Target molecules in a sample can be detected at an improved sensitivity by means of a mass spectrometer. A sample with or without a matrix is placed on a substrate and irradiated with a converged and pulsed primary beam selected from an ion beam, a neutral particle beam or a laser beam. Secondary ions and neutral molecules are emitted along with protons from the irradiated point of the sample as an electric field is applied between the substrate and an extraction electrode disposed above the substrate. A proton-control electrode is arranged in axial symmetry with the trajectory of the primary beam. A voltage is applied thereto so that the generated electric field decelerates the flying protons to raise their adhering efficiency to the flying neutral molecules.
摘要翻译： 可以通过质谱仪以更高的灵敏度检测样品中的靶分子。 将具有或不具有矩阵的样品放置在基底上并用从离子束，中性粒子束或激光束中选择的会聚且脉冲的一次束照射。 随着电场施加在基板和设置在基板之上的引出电极之间，二次离子和中性分子与质子的辐射点一起被发射出来。 质子控制电极与主光束的轨迹轴对称。 施加电压使得所产生的电场使飞行质子减速，以提高它们对飞行中性分子的附着效率。
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29.发明授权Method of analyzing sample using secondary ion emitted from sample and analyzer for performing analysis method 有权使用从样品和分析仪发射的二次离子分析样品进行分析方法的方法公开(公告)号：US09267902B2
公开(公告)日：2016-02-23
申请号：US13979144
申请日：2012-01-12
CPC分类号： G01N23/2202 , H01J49/0468 , H01J49/142
摘要： Provided is a sample analysis method of irradiating a sample with a primary ion beam to analyze a secondary ion emitted from the sample by mass spectrometry, the sample analysis method including the steps of cooling a sample placed in a chamber; forming an ice layer on a surface of the cooled sample by discharging one of water and an aqueous solution to the chamber; and irradiating the surface of the sample with the primary ion beam with the ice layer being formed thereon, wherein an amount of the water forming the ice layer is 0.1 ng/mm2 or more and 20 ng/mm2 or less.
摘要翻译： 提供了一种用一次离子束照射样品以通过质谱分析从样品发射的二次离子的样品分析方法，该样品分析方法包括以下步骤：冷却置于室中的样品; 通过将水和水溶液中的一种排出到所述室，在所述冷却样品的表面上形成冰层; 并且在其上形成有冰层的初级离子束照射样品的表面，其中形成冰层的水的量为0.1ng / mm 2以上且20ng / mm 2以下。
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公开(公告)号：US20130320204A1
公开(公告)日：2013-12-05
申请号：US13979144
申请日：2012-01-12
IPC分类号： G01N23/22
CPC分类号： G01N23/2202 , H01J49/0468 , H01J49/142
摘要： Provided is a sample analysis method of irradiating a sample with a primary ion beam to analyze a secondary ion emitted from the sample by mass spectrometry, the sample analysis method including the steps of cooling a sample placed in a chamber; forming an ice layer on a surface of the cooled sample by discharging one of water and an aqueous solution to the chamber; and irradiating the surface of the sample with the primary ion beam with the ice layer being formed thereon, wherein an amount of the water forming the ice
摘要翻译： 提供了一种用一次离子束照射样品以通过质谱分析从样品发射的二次离子的样品分析方法，该样品分析方法包括以下步骤：冷却置于室中的样品; 通过将水和水溶液中的一种排出到所述室，在所述冷却样品的表面上形成冰层; 并且在其上形成有冰层的初级离子束照射样品的表面，其中形成冰的水量
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