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公开(公告)号：US20150113683A1
公开(公告)日：2015-04-23
申请号：US14507744
申请日：2014-10-06
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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公开(公告)号：US20130198899A1
公开(公告)日：2013-08-01
申请号：US13750977
申请日：2013-01-25
Applicant: Monsanto Technology LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US12195736B2
公开(公告)日：2025-01-14
申请号：US18481464
申请日：2023-10-05
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
IPC: C12N15/82 , A01H4/00 , C12N5/00 , C12N5/04 , C12N9/10 , G01N33/00 , A01H6/20 , A01H6/46 , A01H6/54 , A01H6/60 , C12N1/20 , C12R1/41
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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公开(公告)号：US11807858B2
公开(公告)日：2023-11-07
申请号：US17118539
申请日：2020-12-10
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
IPC: C12N15/82 , C12N5/00 , A01H4/00 , C12N5/04 , G01N33/00 , C12N9/10 , A01H6/20 , A01H6/54 , A01H6/46 , A01H6/60 , C12N1/20 , C12R1/41
CPC classification number: C12N15/8209 , A01H4/003 , A01H4/008 , C12N5/0025 , C12N5/04 , C12N9/1029 , C12N15/8201 , C12N15/8202 , C12N15/8205 , C12N15/8221 , C12N15/8265 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , G01N33/0098 , A01H6/202 , A01H6/4684 , A01H6/542 , A01H6/604 , C12N1/205 , C12N15/8261 , C12R2001/41 , C12Y203/01081
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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公开(公告)号：US20210254084A1
公开(公告)日：2021-08-19
申请号：US17124115
申请日：2020-12-16
Applicant: Monsanto Technology LLC
Inventor： Beth Jo Calabotta , Kevin Lee Depperman , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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Patent Agency Ranking
公开(公告)号：US20210246459A1
公开(公告)日：2021-08-12
申请号：US17118539
申请日：2020-12-10
Applicant: Monsanto Technology LLC
Inventor： Brian J. Martinell , Michael W. Petersen , David A. Somers , Yuechun Wan , Edward Williams , Xudong Ye
Abstract: The present invention relates to methods and compositions for transforming soybean, corn, cotton, or canola explants using spectinomycin as a selective agent for transformation of the explants. The method may further comprise treatment of the explants with cytokinin during the transformation and regeneration process.
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公开(公告)号：US10584345B2
公开(公告)日：2020-03-10
申请号：US15706427
申请日：2017-09-15
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US20180135063A1
公开(公告)日：2018-05-17
申请号：US15706427
申请日：2017-09-15
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US09790512B2
公开(公告)日：2017-10-17
申请号：US14642671
申请日：2015-03-09
Applicant: MONSANTO TECHNOLOGY LLC
Inventor： Beth Jo Calabotta , Kevin Lee Deppermann , Erik D. Dersch , Jerald D. Heise , Angela Ranae Koestel , Cindy L. Ludwig , Brian J. Martinell , Edward Williams
CPC classification number: C12N15/8201 , A01H4/003 , A01H4/008 , C12N5/04 , C12N9/1029 , C12N15/8202 , C12N15/8205 , C12N15/8209 , C12N15/8271 , C12N15/8274 , C12N15/8275 , C12N15/8277 , C12N15/8281 , C12Y203/01081 , G01N33/0098
Abstract: The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.
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公开(公告)号：US20150040266A1
公开(公告)日：2015-02-05
申请号：US14298739
申请日：2014-06-06
Applicant: Monsanto Technology LLC
Inventor： Xudong Ye , Junjiang Shen , Edward Williams
IPC: C12N15/82
CPC classification number: C12N15/8202 , C12N15/8205 , C12R1/41 , G01N33/5097
Abstract: The invention relates to methods for Rhizobia-mediated genetic transformation of plant cells, including soybean, canola, corn, and cotton cells. These include both VirD2-dependent and VirD2-independent methods. Bacterial species utilized include strains of Rhizobium sp., Sinorhizobium sp., and Mesorhizobium sp. Vectors for use in such transformation are also disclosed.
Abstract translation: 本发明涉及用于根瘤菌介导的植物细胞遗传转化的方法，包括大豆，卡诺拉油菜，玉米和棉花细胞。 这些包括依赖于VirD2和与VirD2无关的方法。 使用的细菌种类包括根瘤菌属，中华根瘤菌属（Sinorhizobium sp。）和中生根瘤菌属（Mesorhizobium sp。 还公开了用于这种转化的载体。
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