公开(公告)号：US20130147613A1

公开(公告)日：2013-06-13

申请号：US13814910

申请日：2011-08-24

申请人： Xiaobing Guo ,  Yi Xiao ,  Wei Xie ,  Dengfeng Wu

发明人： Xiaobing Guo ,  Yi Xiao ,  Wei Xie ,  Dengfeng Wu

IPC分类号： G08C19/16

CPC分类号： G08C19/16 ,  G08C17/00

摘要： An electronic device (100) and method for operating the same. The electronic device (100) comprises at least two working modes and comprises a connection state acquisition unit (110) and a mode switching unit (12). The connection state acquisition unit (110) is used for acquiring the connection state between the electronic device (100) and a remote control unit, and the remote control unit is used for remotely controlling the electronic device (100). The mode switching unit (12) is used for switching the working mode of the electronic device (100) according to the connected state.

摘要翻译： 一种电子设备（100）及其操作方法。 电子设备（100）包括至少两个工作模式，并且包括连接状态获取单元（110）和模式切换单元（12）。 连接状态获取单元（110）用于获取电子设备（100）和遥控单元之间的连接状态，并且远程控制单元用于遥控电子设备（100）。 模式切换单元（12）用于根据连接状态切换电子设备（100）的工作模式。

公开(公告)号：US20070254282A1

公开(公告)日：2007-11-01

申请号：US10588153

申请日：2005-01-27

申请人： Itamar Willner ,  Yi Xiao ,  Valeri Pavlov ,  Tamara Niazov

发明人： Itamar Willner ,  Yi Xiao ,  Valeri Pavlov ,  Tamara Niazov

IPC分类号： C12Q1/68

CPC分类号： C12N15/115 ,  C12Q1/6813 ,  C12Q1/683 ,  C12Q2521/543 ,  C12Q2521/331

摘要： A method for the determination of an analyte in a sample, the method comprising: (a) providing a catalytic polynucleotide; (b) contacting the catalytic polynucleotide with the sample so that the catalytic polynucleotide may bind to the analyte; (c) providing assay conditions such that the catalytic polynucleotide produces an optically detectable signal in the presence of the analyte; and (d) detecting the signal, thereby determining the presence of the analyte in the sample. One application of the method is for the detection of telomerase in a biological sample.

摘要翻译： 一种用于测定样品中分析物的方法，所述方法包括：（a）提供催化多核苷酸; （b）使催化多核苷酸与样品接触，使得催化多核苷酸可以结合分析物; （c）提供测定条件，使得催化多核苷酸在分析物存在下产生可光学检测的信号; 和（d）检测信号，从而确定样品中分析物的存在。 该方法的一个应用是用于检测生物样品中的端粒酶。

公开(公告)号：US11796499B2

公开(公告)日：2023-10-24

申请号：US18065118

申请日：2022-12-13

申请人： Yi Xiao ,  Yingzhu Liu ,  Juan Canoura

发明人： Yi Xiao ,  Yingzhu Liu ,  Juan Canoura

IPC分类号： G01N27/327 ,  G01N33/543 ,  G01N33/53

CPC分类号： G01N27/3276 ,  G01N27/3277 ,  G01N33/5308 ,  G01N33/5438

摘要： The subject invention provides methods for fabricating electrochemical aptamer-based (E-AB) sensors with enhanced sensitivity, signal-to-noise ratios, LOD, and improved stability and reproducibility. The subject invention also provides methods for aptamer immobilization on the surface of the electrode, which favors sufficient spacing between aptamers at the microscale to achieve optimal target recognition, folding, and signal transduction. The E-AB sensors of the subject invention provide superior sensing regardless of the sequence or structure of the bound aptamers or the physiochemical properties of the target.

公开(公告)号：US11579110B2

公开(公告)日：2023-02-14

申请号：US17826891

申请日：2022-05-27

申请人： Yi Xiao ,  Juan Canoura

发明人： Yi Xiao ,  Juan Canoura

IPC分类号： C12N15/115 ,  G01N27/327 ,  G01N21/64

摘要： The subject invention provides materials and methods for single-step fluorescence and electrochemical detection of small molecules, e.g., fentanyl and its analogs, in a sample. The subjection invention provides nucleic acids materials, e.g., aptamers (nucleic acid oligonucleotides) that can bind to fentanyl and its analogs with nanomolar affinity and high specificity against illicit drugs, adulterants, and cutting agents commonly existing in seized samples. The method for detecting fentanyl and/or its analogs in a sample comprises contacting the sample with an aptamer-based sensor selective for fentanyl and its analogs, and sensitively, specifically, and rapidly detecting fentanyl and/or its analogs in the sample.

公开(公告)号：US11060095B2

公开(公告)日：2021-07-13

申请号：US17117317

申请日：2020-12-10

申请人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

发明人： Weijuan Yang ,  Haixiang Yu ,  Yingzhu Liu ,  Yi Xiao

IPC分类号： C12N15/11 ,  C12N15/115 ,  A61K31/167 ,  A61K31/137 ,  G01N27/26

摘要： The subject invention provides a SELEX strategy for isolating cross-reactive aptamers that recognize a core structure of a small-molecule family and bind to several structurally-similar molecules in the family. The subject invention also provides methods, assays, and products for detecting small-molecule targets of the family in a sample in both clinical and field settings. Such method is based on an aptamer sensor that reports the presence of small-molecule targets via a sensitive colorimetric signal for naked-eye detection. The subject invention further provides exonuclease-based methods for generating structure-switching aptamers from fully folded aptamers and developing electrochemical aptamer-based (E-AB) sensors for rapid and sensitive detection of synthetic cathinones.

公开(公告)号：US10725058B2

公开(公告)日：2020-07-28

申请号：US16179336

申请日：2018-11-02

申请人： Yi Xiao ,  Yingping Luo ,  Haixiang Yu

发明人： Yi Xiao ,  Yingping Luo ,  Haixiang Yu

IPC分类号： G01N33/94 ,  G01N33/52

摘要： The subject invention provides methods, assays, and products for visual detection of small-molecule targets in a sample in both clinical and field settings within minutes. The subject invention is based on an aptamer sensor that reports the presence of small-molecule target via a sensitive colorimetric signal for naked-eye detection. The aptamer sensor is a CBSAzyme-based sensor having both target-mediated cooperative behavior of the CBSA and peroxidase-mimicking catalytic activity of DNAzyme. The subject invention also provides methods of using the CBSAzyme-based sensor.

公开(公告)号：US20160329121A1

公开(公告)日：2016-11-10

申请号：US15146440

申请日：2016-05-04

申请人： Yi Xiao ,  Bhargav Guntupalli ,  Janak Paudyal

发明人： Yi Xiao ,  Bhargav Guntupalli ,  Janak Paudyal

IPC分类号： H01B1/04 ,  H01B1/02

CPC分类号： H01B1/04 ,  H01B1/02

摘要： A porous conductive thin film includes a layer of metal nanoparticles decorated on a layer of conductive carbon nanomaterials. The thin film can be supported by a porous support. The porous support can be a MCE paper upon which a metallic or semi-metallic single-walled carbon nanotube (SWCNT) layer is decorated with gold nanoparticles (AuNPs) or platinum nanoparticles (PtNPs). The thin film can be constructed by filtering a dispersion of SWCNTs onto MCE filter paper followed by the filtration of a citrate stabilized dispersion of AuNPs or PtNPs onto the SWCNT layer.

摘要翻译： 多孔导电薄膜包括在导电碳纳米材料层上装饰的一层金属纳米颗粒。 薄膜可以由多孔载体支撑。 多孔载体可以是MCE纸，金属或半金属单壁碳纳米管（SWCNT）层用金纳米颗粒（AuNP）或铂纳米颗粒（PtNP）进行装饰。 可以通过将SWCNT的分散体过滤到MCE滤纸上，然后将AuNP或PtNP的柠檬酸盐稳定分散体过滤到SWCNT层上来构建薄膜。

公开(公告)号：US07803542B2

公开(公告)日：2010-09-28

申请号：US11564674

申请日：2006-11-29

申请人： Yi Xiao ,  Arica Lubin ,  Kevin Plaxco

发明人： Yi Xiao ,  Arica Lubin ,  Kevin Plaxco

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6825 ,  G01N27/3277 ,  C12Q2565/133 ,  C12Q2563/113 ,  C12Q2525/205 ,  C12Q2537/163 ,  C12Q2537/137 ,  C12Q2565/525 ,  C12Q2565/549 ,  C12Q2565/607

摘要： The invention provides a general “signal-on” architecture for oligonucleotide-based detectors that leads to order of magnitude increases in signal gain and sensitivity as compared to prior art detectors. The detectors of the invention rely on base pairing between two oligonucleotide strands, the sensor strand and the blocker strand. In the ‘off’ position of the detector, i.e., in the absence of target, the blocker strand and sensor strand are base-paired. As shown in FIG. 1, the formation of comparatively rigid, duplex DNA prevents the redox moiety from approaching the electrode surface, thereby suppressing Faradaic currents. When target is added to the system, the target displaces the blocker strand, binds to the sensor strand, liberating the end of the redox-labeled oligonucleotide to produce a flexible element. This, in turn, allows the redox moiety to collide with the electrode surface, producing a readily detectable Faradaic current.

摘要翻译： 本发明提供了一种基于寡核苷酸的检测器的通用“信号启动”架构，与现有技术的检测器相比，信号增益和灵敏度的幅度增加了一个数量级。 本发明的检测器依赖于两个寡核苷酸链之间的碱基配对，传感器链和阻断链。 在检测器的“关闭”位置，即在没有靶的情况下，阻滞链和传感器链是碱基配对的。 如图所示。 1，相对刚性的双链DNA的形成防止氧化还原部分接近电极表面，从而抑制法拉第电流。 当将靶标加入到系统中时，靶标取代阻断链，结合传感器链，释放氧化还原标记的寡核苷酸的末端以产生柔性元件。 这又使得氧化还原部分与电极表面碰撞，产生易于检测的法拉第电流。

公开(公告)号：US20070154909A1

公开(公告)日：2007-07-05

申请号：US11564674

申请日：2006-11-29

申请人： Yi Xiao ,  Arica Lubin ,  Kevin Plaxco

发明人： Yi Xiao ,  Arica Lubin ,  Kevin Plaxco

IPC分类号： C12Q1/68 ,  C12M1/34

CPC分类号： C12Q1/6825 ,  G01N27/3277 ,  C12Q2565/133 ,  C12Q2563/113 ,  C12Q2525/205 ,  C12Q2537/163 ,  C12Q2537/137 ,  C12Q2565/525 ,  C12Q2565/549 ,  C12Q2565/607

摘要： The invention provides a general “signal-on” architecture for oligonucleotide-based detectors that leads to order of magnitude increases in signal gain and sensitivity as compared to prior art detectors. The detectors of the invention rely on base pairing between two oligonucleotide strands, the sensor strand and the blocker strand. In the ‘off’ position of the detector, i.e., in the absence of target, the blocker strand and sensor strand are base-paired. As shown in FIG. 1, the formation of comparatively rigid, duplex DNA prevents the redox moiety from approaching the electrode surface, thereby suppressing Faradaic currents. When target is added to the system, the target displaces the blocker strand, binds to the sensor strand, liberating the end of the redox-labeled oligonucleotide to produce a flexible element. This, in turn, allows the redox moiety to collide with the electrode surface, producing a readily detectable Faradaic current.

摘要翻译： 本发明提供了一种基于寡核苷酸的检测器的通用“信号启动”架构，与现有技术的检测器相比，信号增益和灵敏度的幅度增加了一个数量级。 本发明的检测器依赖于两个寡核苷酸链之间的碱基配对，传感器链和阻断链。 在检测器的“关闭”位置，即在没有靶的情况下，阻滞链和传感器链是碱基配对的。 如图所示。 1，相对刚性的双链DNA的形成防止氧化还原部分接近电极表面，从而抑制法拉第电流。 当将靶标加入到系统中时，靶标取代阻断链，结合传感器链，释放氧化还原标记的寡核苷酸的末端以产生柔性元件。 这又使得氧化还原部分与电极表面碰撞，产生易于检测的法拉第电流。

公开(公告)号：US11656235B2

公开(公告)日：2023-05-23

申请号：US17396663

申请日：2021-08-07

申请人： Yi Xiao ,  Obtin Alkhamis ,  Haixiang Yu

发明人： Yi Xiao ,  Obtin Alkhamis ,  Haixiang Yu

IPC分类号： G01N33/94 ,  G01N21/78 ,  G01N33/58

CPC分类号： G01N33/9486 ,  G01N21/78 ,  G01N33/583 ,  G01N33/946 ,  G01N33/948 ,  G01N33/9413

摘要： The subject invention provides materials and methods for single-step detection of target molecules in a sample. The methods and assays of the subject invention employ a dye-displacement strategy, in which aptamers complexed with a cyanine dye for sensitive and rapid detection of targets of interest. In the presence of a target, aptamer-target binding liberates the non-covalently bound aptamer-binding dye, resulting in optical changes that can be observed spectrophotometrically or with the naked eye. The methods and assays of the subject invention enable the colorimetric detection of targets of interest regardless of their structure, sequence, target-binding affinity, and physicochemical properties of their targets.