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公开(公告)号：US08232086B2
公开(公告)日：2012-07-31
申请号：US13010219
申请日：2011-01-20
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质，例如 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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公开(公告)号：US20080299613A1
公开(公告)日：2008-12-04
申请号：US12130838
申请日：2008-05-30
申请人： Sandra Merino , Keith McFarland , Joel Cherry , Sarah Teter
发明人： Sandra Merino , Keith McFarland , Joel Cherry , Sarah Teter
CPC分类号： C12N9/2437 , C07K14/37 , C12N9/2445 , C12P7/10 , C12P19/14 , C12P19/16 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions.
摘要翻译： 本发明涉及用于降解或转化含纤维素材料的纤维素分解组合物以及制备和使用该组合物的方法。
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公开(公告)号：US07354743B2
公开(公告)日：2008-04-08
申请号：US11036871
申请日：2005-01-14
申请人： Elena Vlasenko , Joel Cherry , Feng Xu
发明人： Elena Vlasenko , Joel Cherry , Feng Xu
IPC分类号： C12P19/04
CPC分类号： C12P19/14 , C12P7/06 , C12P13/04 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermentating microoganisms; and (c) recovering the organic substance from the fermentation.
摘要翻译： 本发明涉及降解木质纤维素材料的方法，包括：在至少一种表面活性剂存在下，用有效量的一种或多种纤维素分解酶处理木质纤维素材料，所述表面活性剂选自仲醇乙氧基化物，脂肪醇乙氧基化物 ，壬基酚乙氧基化物，十三烷基乙氧基化物和聚氧乙烯醚，其中与不存在表面活性剂相比，表面活性剂的存在增加了木质纤维素材料的降解。 本发明还涉及生产有机物质的方法，其包括：（a）在至少一种表面活性剂存在下，用有效量的一种或多种纤维素分解酶对木质纤维素材料进行糖化，所述表面活性剂选自仲醇乙氧基化物 ，脂肪醇乙氧基化物，壬基酚乙氧基化物，十三烷基乙氧基化物和聚氧乙烯醚，其中与不存在表面活性剂相比，表面活性剂的存在增加了木质纤维素材料的降解; （b）用一种或多种发酵微生物发酵步骤（a）的糖化木质纤维素材料; 和（c）从发酵中回收有机物质。
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公开(公告)号：US20070270574A1
公开(公告)日：2007-11-22
申请号：US11736322
申请日：2007-04-17
申请人： Jon Ness , Mark Welch , Lorraine Giver , Joel Cherry , Torben Borchert , Jeremy Minshull
发明人： Jon Ness , Mark Welch , Lorraine Giver , Joel Cherry , Torben Borchert , Jeremy Minshull
IPC分类号： C07K14/00
CPC分类号： C12N9/6408 , C12N9/54
摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.
摘要翻译： 提供了新的枯草杆菌蛋白酶同源物（核酸和蛋白质）。 还提供了包括这些新蛋白质，重组细胞，涉及新同系物的洗牌方法，新同系物的抗体以及使用同源物的方法的组合物。
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公开(公告)号：US06876932B1
公开(公告)日：2005-04-05
申请号：US09607142
申请日：2000-06-29
CPC分类号： C12N9/2417 , A21D2/26 , A21D8/042
摘要： The inventors have modified the amino acid sequence of a maltogenic alpha-amylase to obtain variants with improved properties, based on the three-dimensional structure of the maltogenic alpha-amylase Novamyl. The variants have altered physicochemical properties., e.g. an altered pH optimum, improved thermostability, increased specific activity, an altered cleavage pattern or an increased ability to reduce retrogradation of starch or staling of bread.
摘要翻译： 基于麦芽糖α-淀粉酶Novamyl的三维结构，本发明人已经修饰了产麦芽糖α-淀粉酶的氨基酸序列以获得具有改进性质的变体。 这些变体具有改变的物理化学性质。 改变的pH最佳，改善的热稳定性，增加的比活性，改变的切割模式或增加降低淀粉退化或面包陈化的能力。
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公开(公告)号：US09969993B2
公开(公告)日：2018-05-15
申请号：US12130838
申请日：2008-05-30
申请人： Sandra Merino , Keith McFarland , Joel Cherry , Sarah Teter
发明人： Sandra Merino , Keith McFarland , Joel Cherry , Sarah Teter
CPC分类号： C12N9/2437 , C07K14/37 , C12N9/2445 , C12P7/10 , C12P19/14 , C12P19/16 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to cellulolytic compositions for degrading or converting cellulose-containing material and methods of producing and using the compositions.
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27.发明授权Methods for transforming and expression screening of filamentous fungal cells with a DNA library 有权用DNA文库转化和表达筛选丝状真菌细胞的方法公开(公告)号：US09045748B2
公开(公告)日：2015-06-02
申请号：US11140150
申请日：2005-05-26
申请人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
发明人： Sarah Teter , Michael Lamsa , Joel Cherry , Connie Ward
CPC分类号： C12N15/1037 , C12N15/1086 , C12N15/80 , C40B40/02
摘要： The present invention relates to methods for expression screening of filamentous fungal transformants, comprising: (a) isolating single colony transformants of a DNA library introduced into E. coli; (b) preparing DNA from each of the single colony E. coli transformants; (c) introducing a sample of each of the DNA preparations of step (b) into separate suspensions of protoplasts of a filamentous fungus to obtain transformants thereof, wherein each transformant contains one or more copies of an individual polynucleotide from the DNA library; (d) growing the individual filamentous fungal transformants of step (c) on selective growth medium, thereby permitting growth of the filamentous fungal transformants, while suppressing growth of untransformed filamentous fungi; and (e) measuring activity or a property of each polypeptide encoded by the individual polynucleotides. The present invention also relates to isolated polynucleotides encoding polypeptides of interest obtained by such methods, to nucleic acid constructs, expression vectors, and recombinant host cells comprising the isolated polynucleotides, and to methods of producing the polypeptides encoded by the isolated polynucleotides.
摘要翻译： 本发明涉及丝状真菌转化体的表达筛选方法，其包括：（a）分离导入大肠杆菌的DNA文库的单个菌落转化体; （b）从每个单个菌落的大肠杆菌转化体制备DNA; （c）将步骤（b）的每种DNA制剂的样品引入到丝状真菌的原生质体的分离悬浮液中以获得其转化体，其中每个转化体含有来自DNA文库的单个多核苷酸的一个或多个拷贝; （d）在选择性生长培养基上生长步骤（c）的单个丝状真菌转化体，从而允许丝状真菌转化体生长，同时抑制未转化的丝状真菌的生长; 和（e）测量由各个多核苷酸编码的每种多肽的活性或性质。 本发明还涉及通过这些方法获得的编码感兴趣的多肽的分离的多核苷酸，包括分离的多核苷酸的核酸构建体，表达载体和重组宿主细胞，以及由分离的多核苷酸编码的多肽的制备方法。
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公开(公告)号：US20090215128A1
公开(公告)日：2009-08-27
申请号：US11821438
申请日：2007-06-22
申请人： Elena Vlasenko , Joel Cherry , Feng Xu
发明人： Elena Vlasenko , Joel Cherry , Feng Xu
CPC分类号： C12P19/14 , C12P7/06 , C12P13/04 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for degrading a lignocellulosic material, comprising: treating the lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying a lignocellulosic material with an effective amount of one or more cellulolytic enzymes in the presence of at least one surfactant selected from the group consisting of a secondary alcohol ethoxylate, fatty alcohol ethoxylate, nonylphenol ethoxylate, tridecyl ethoxylate, and polyoxyethylene ether, wherein the presence of the surfactant increases the degradation of lignocellulosic material compared to the absence of the surfactant; (b) fermenting the saccharified lignocellulosic material of step (a) with one or more fermenting microorganisms; and (c) recovering the organic substance from the fermentation.
摘要翻译： 本发明涉及降解木质纤维素材料的方法，包括：在至少一种表面活性剂存在下，用有效量的一种或多种纤维素分解酶处理木质纤维素材料，所述表面活性剂选自仲醇乙氧基化物，脂肪醇乙氧基化物 ，壬基酚乙氧基化物，十三烷基乙氧基化物和聚氧乙烯醚，其中与不存在表面活性剂相比，表面活性剂的存在增加了木质纤维素材料的降解。 本发明还涉及生产有机物质的方法，其包括：（a）在至少一种表面活性剂存在下，用有效量的一种或多种纤维素分解酶对木质纤维素材料进行糖化，所述表面活性剂选自仲醇乙氧基化物 ，脂肪醇乙氧基化物，壬基酚乙氧基化物，十三烷基乙氧基化物和聚氧乙烯醚，其中与不存在表面活性剂相比，表面活性剂的存在增加了木质纤维素材料的降解; （b）用一种或多种发酵微生物发酵步骤（a）的糖化木质纤维素材料; 和（c）从发酵中回收有机物质。
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公开(公告)号：US20080293109A1
公开(公告)日：2008-11-27
申请号：US12172852
申请日：2008-07-14
申请人： Randy Berka , Joel Cherry
发明人： Randy Berka , Joel Cherry
IPC分类号： C12P13/24 , C12P1/00 , C12P7/04 , C12P7/00 , C12P13/04 , C12P7/16 , C12P7/10 , C12P13/06 , C12P13/08 , C12P13/10 , C12P13/12 , C12P13/14 , C12P13/16 , C12P13/18 , C12P13/20
CPC分类号： C12P19/14 , C12P5/023 , C12P7/00 , C12P7/02 , C12P7/04 , C12P7/06 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/26 , C12P7/28 , C12P7/40 , C12P13/04 , C12P17/04 , C12P19/02 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
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公开(公告)号：US07413882B2
公开(公告)日：2008-08-19
申请号：US11078921
申请日：2005-03-10
申请人： Randy Berka , Joel Cherry
发明人： Randy Berka , Joel Cherry
CPC分类号： C12P19/14 , C12P5/023 , C12P7/00 , C12P7/02 , C12P7/04 , C12P7/06 , C12P7/10 , C12P7/16 , C12P7/18 , C12P7/20 , C12P7/26 , C12P7/28 , C12P7/40 , C12P13/04 , C12P17/04 , C12P19/02 , Y02E50/10 , Y02E50/16 , Y02E50/17 , Y02E50/343
摘要： The present invention relates to methods for converting plant cell wall polysaccharides into one or more products, comprising: treating the plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into the one or more products. The present invention also relates to methods for producing an organic substance, comprising: (a) saccharifying plant cell wall polysaccharides with an effective amount of a spent whole fermentation broth of a recombinant microorganism, wherein the recombinant microorganism expresses one or more heterologous genes encoding enzymes which degrade or convert the plant cell wall polysaccharides into saccharified material; (b) fermenting the saccharified material of step (a) with one or more fermenting microoganisms; and (c) recovering the organic substance from the fermentation.
摘要翻译： 本发明涉及将植物细胞壁多糖转化为一种或多种产品的方法，其包括：用有效量的重组微生物的消耗的全发酵液处理植物细胞壁多糖，其中重组微生物表达一种或多种异源 编码将植物细胞壁多糖降解或转化成一种或多种产物的酶的基因。 本发明还涉及有机物质的制造方法，其特征在于，包括：（a）用有效量的重组微生物用的全部发酵肉汤糖化植物细胞壁多糖，其中所述重组微生物表达一种或多种编码酶的异源基因 其将植物细胞壁多糖降解或转化为糖化物质; （b）用一种或多种发酵微生物发酵步骤（a）的糖化物质; 和（c）从发酵中回收有机物质。
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