Allele specific primer extension
    24.
    发明授权
    Allele specific primer extension 有权
    等位基因特异性引物延伸

    公开(公告)号:US07560254B2

    公开(公告)日:2009-07-14

    申请号:US10651558

    申请日:2003-08-29

    摘要: A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3′→5′ exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.

    摘要翻译: 提供了表征核酸样品的方法,其包括以下步骤:(a)进行DNA聚合酶反应,其包括模板,等位基因特异性引物,至少一个末端磷酸酯标记的核苷酸,DNA聚合酶和 任选地当引物不可水解时具有3'→5'核酸外切酶活性的酶,该反应导致标记的多磷酸盐的产生; (b)允许标记的多磷酸酯与磷酸酶反应以产生可检测的物质; (c)检测可检测物种; 和(d)基于这种检测来表征核酸样品。

    Amplification of self-ligated, circularized cDNA for expression profiling
    25.
    发明授权
    Amplification of self-ligated, circularized cDNA for expression profiling 有权
    用于表达谱的自连接的环化cDNA的扩增

    公开(公告)号:US07309571B2

    公开(公告)日:2007-12-18

    申请号:US10770657

    申请日:2004-02-03

    申请人: John Nelson

    发明人: John Nelson

    IPC分类号: C12Q1/68 C12P19/34

    摘要: This invention presents a new cDNA amplification method. RNA is first converted to cDNA. The synthesis of cDNA can include a promoter tagged oligonucleotide, and then this cDNA is ligated to form circles (or possibly concatemers). This is then amplified using a Phi29 DNA polymerase based rolling circle and strand displacement amplification. The invention allows for RNA promoter sequences to be attached to the cDNA to facilitate additional amplification through the generation of RNA from the amplified cDNA. The resulting product can then be used to make materials for gene expression studies or other RNA analysis procedures.

    摘要翻译: 本发明提出了一种新的cDNA扩增方法。 RNA首先转化为cDNA。 cDNA的合成可以包括启动子标记的寡核苷酸,然后将该cDNA连接形成环(或可能是连接体)。 然后使用基于Phi29 DNA聚合酶的滚动圆和链置换扩增来扩增。 本发明允许将RNA启动子序列连接到cDNA,以便通过从扩增的cDNA产生RNA来进一步扩增。 所得产物可用于制备用于基因表达研究或其他RNA分析程序的材料。

    INSECT CONTROL COMPOSITIONS
    26.
    发明申请
    INSECT CONTROL COMPOSITIONS 审中-公开
    防护组合物

    公开(公告)号:US20070264294A1

    公开(公告)日:2007-11-15

    申请号:US11382574

    申请日:2006-05-10

    IPC分类号: A01N25/00

    摘要: Disclosed herein are sprayable insect repellent compositions that provide improved skin feel through the incorporation of a powder ingredient. The powder is essentially permanently suspended in the composition by a thickener (e.g. carbomer thickener) so that no shaking to resuspend settled out powder is necessary immediately before the composition is applied to the skin. This is achieved in an aerosol or spritz spray formulation, while avoiding clogging problems. One composition contains ethanol, DEET or picaridin, carbomer thickener, a neutralizer, corn starch powder, water, and gas propellant.

    摘要翻译: 本文公开了可喷雾的驱虫剂组合物,其通过掺入粉末成分来提供改善的皮肤感觉。 该粉末通过增稠剂(例如卡波姆增稠剂)基本上永久地悬浮在组合物中,使得在将组合物施用于皮肤之前不需要立即振荡以重悬沉淀出的粉末。 这可以在气雾剂或喷雾剂喷雾配方中实现,同时避免堵塞问题。 一种组合物含有乙醇,DEET或氨基吡啶,卡波姆增稠剂,中和剂,玉米淀粉粉末,水和气体推进剂。

    Stone fabrication positioning system
    27.
    发明申请
    Stone fabrication positioning system 审中-公开
    石材制造定位系统

    公开(公告)号:US20070161331A1

    公开(公告)日:2007-07-12

    申请号:US11595378

    申请日:2006-11-10

    申请人: John Nelson

    发明人: John Nelson

    IPC分类号: B24B51/00 B24B7/30

    CPC分类号: B24B41/068 B24B9/06 B28D7/04

    摘要: A stone cutting system effective for mechanically delineating defined datum points in a Cartesian coordinate system by positioning at least one datum point defining means per coordinate comprising a combination blocking and clamping device, a table of a computer numerically controlled (CNC) stoneworking machine, a locating means positioned on the table, the blocking and clamping device being in the form of a parallelepiped including a bottom surface having a plurality of apertures adapted to enable positioning about the locating means, a top surface having a first blocking edge and a second clamping edge, including at least one means for clamping a workpiece within said top surface second clamping edge, a left side surface, a right side surface, a front surface, a rear surface, and at least one means for fixation of said device on said table.

    摘要翻译: 一种石材切割系统,其有效地通过定位至少一个基准点来定义笛卡尔坐标系中的定义的基准点,每个坐标包括组合阻塞和夹紧装置,计算机数字控制(CNC)制作机的工作台,定位 位于桌子上的装置,阻塞和夹紧装置为平行六面体形式,包括底表面,底面具有多个适于能够围绕定位装置定位的孔口,顶表面具有第一阻挡边缘和第二夹紧边缘, 包括用于将工件夹持在所述顶表面第二夹紧边缘,左侧表面,右侧表面,前表面,后表面中的至少一个装置以及用于将所述装置固定在所述工作台上的至少一个装置。

    Microreplicated article with moire reducing surface
    29.
    发明申请
    Microreplicated article with moire reducing surface 审中-公开
    具有莫尔条纹表面的微复制品

    公开(公告)号:US20060209428A1

    公开(公告)日:2006-09-21

    申请号:US11369482

    申请日:2006-03-06

    IPC分类号: G02B3/08

    摘要: A microreplicated article having a moiré reducing surface and method of manufacturing the same, are disclosed. A microreplicated article includes a flexible substrate having first and second opposed surfaces, a first coated microreplicated pattern on the first surface, and a second coated microreplicated pattern on the second surface. The first coated microreplicated pattern and the second coated microreplicated pattern are registered to within 10 micrometers in a machine direction and a transverse direction and the first coated microreplicated pattern and second coated microreplicated pattern form a plurality of lens segments. Each lens segment includes a plurality of lens elements each having an optical axis where all of the lens element optical axes are parallel to each other and lens elements within a first lens segment have optical axes that are offset from optical axes of lens elements within an adjacent second lens segment.

    摘要翻译: 公开了具有莫尔面还原表面的微复制品及其制造方法。 微复制品包括具有第一和第二相对表面的柔性基底,第一表面上的第一涂覆的微复制图案和在第二表面上的第二涂覆的微复制图案。 第一涂布的微复制图案和第二涂布的微复制图案在机器方向和横向方向上记录在10微米内,并且第一涂布的微复制图案和第二涂布的微复制图案形成多个透镜段。 每个透镜段包括多个透镜元件,每个透镜元件具有其中所有透镜元件光轴彼此平行的光轴,并且第一透镜段内的透镜元件具有偏离相邻的透镜元件的透镜元件的光轴的光轴 第二透镜段。