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21.发明授权
公开(公告)号：US5916777A
公开(公告)日：1999-06-29
申请号：US949076
申请日：1997-10-10
摘要： The present invention relates to methods of enzymatically synthesizing oligonucleotides. In particular, it relates to the use of 3'-ribonucleotide primers which effectively serve to initiate oligonucleotide synthesis, and can also be easily cleaved from synthesis products and reused in subsequent synthesis reactions.
摘要翻译： 本发明涉及酶促合成寡核苷酸的方法。 特别涉及使用有效用于引发寡核苷酸合成的3'-核糖核苷酸引物，也可以容易地从合成产物中切割出来，并在随后的合成反应中重复使用。
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公开(公告)号：US5851548A
公开(公告)日：1998-12-22
申请号：US482305
申请日：1995-06-07
IPC分类号： A61K9/127 , C07C381/12 , C12N15/88
CPC分类号： A61K9/1272 , A61K47/48046 , C07C381/12 , C12N15/88
摘要： Lipid molecules bearing a cationic charge are described. These cationic lipids are useful in the delivery of biomolecules, such as oligonucleotides, nucleic acids, peptides, diagnostic imaging agents, proteins and drug molecules. In the form of liposomes, they can effectively be used for the intracellular delivery of biomolecules for therapeutic or diagnostic purposes.
摘要翻译： 描述了带有阳离子电荷的脂质分子。 这些阳离子脂质可用于递送生物分子，如寡核苷酸，核酸，肽，诊断成像剂，蛋白质和药物分子。 以脂质体的形式，它们可以有效地用于生物分子的细胞内递送用于治疗或诊断目的。
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23.发明授权Method for the intracellular delivery of biomolecules using liposomes containing cationic lipids and vitamin D 失效使用含有阳离子脂质和维生素D的脂质体细胞内转运生物分子的方法
公开(公告)号：US5711964A
公开(公告)日：1998-01-27
申请号：US480622
申请日：1995-06-07
IPC分类号： A61K9/127 , C07C381/12 , C12N15/88
CPC分类号： C12N15/88 , A61K47/48046 , A61K9/1272 , C07C381/12
摘要： Lipid molecules bearing a cationic charge are described. These cationic lipids are useful in the delivery of biomolecules, such as oligonucleotides, nucleic acids, peptides, diagnostic imaging agents, proteins and drug molecules. In the form of liposomes, they can effectively be used for the intracellular delivery of biomolecules for therapeutic or diagnostic purposes.
摘要翻译： 描述了带有阳离子电荷的脂质分子。 这些阳离子脂质可用于递送生物分子，如寡核苷酸，核酸，肽，诊断成像剂，蛋白质和药物分子。 以脂质体的形式，它们可以有效地用于生物分子的细胞内递送用于治疗或诊断目的。
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公开(公告)号：US4734363A
公开(公告)日：1988-03-29
申请号：US675386
申请日：1984-11-27
CPC分类号： C07H21/00 , C12Q1/686 , C12Q1/6876
摘要： A process for production of a single strand of a nucleic acid comprising covalently linking to a solid substrate a polynucleotide complementary to the desired strand, hybridizing said polynucleotide with an oligonucleotide, extending the oligonucleotide in direction away from said substrate, denaturing the hybridized polynucleotide and extended oligonucleotide, thereby to free the extended oligonucleotide from the solid substrate, and separating the extended oligonucleotide. The product can be used for making analytical probes.
摘要翻译： 一种生产单链核酸的方法，包括共价连接到固体底物与所需链互补的多核苷酸，将所述多核苷酸与寡核苷酸杂交，使寡核苷酸沿远离所述底物的方向延伸，使杂交的多核苷酸变性并扩展 从而从固体底物中释放延伸的寡核苷酸，并分离延伸的寡核苷酸。 该产品可用于制造分析探针。
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公开(公告)号：US4713326A
公开(公告)日：1987-12-15
申请号：US611667
申请日：1984-05-18
IPC分类号： C09H1/00 , C07H21/00 , C07H21/04 , C08H1/00 , C12Q1/68 , G01N33/53 , G01N33/547 , G01N33/548 , G01N33/50
CPC分类号： C07H21/00 , C12Q1/6834 , G01N33/548 , Y10T436/143333
摘要： A solid support capable of binding a nucleic acid thereto upon suitable irradiation, comprising (a) a solid substrate, (b) a photochemically reactive intercalator compound or other nucleic acid-binding ligands, and (c) divalent radical chemically linking the substrate and the ligand (b). Specifically, a hydroxy group-containing solid substrate such as nitrocellulose paper is linked via a bifunctional reagent such as cyanogen bromide or 1,4-butanedioldiglycidyl ether to an amino-substituted angelicin or psoralen or ethidium bromide which in turn is photochemically linked to a nucleic acid. The resulting immobilized nucleic acid probe is capable of hybridizing with complementary nucleic acid fragments and is thereby useful in diagnostic assays.
摘要翻译： 包含（a）固体底物，（b）光化学反应性嵌入剂化合物或其它核酸结合配体的能够将核酸与其结合的固体支持物，和（c）将基材和 配体（b）。 具体地说，将含有羟基的固体基质如硝化纤维素纸通过诸如溴化氰或1,4-丁二醇二缩水甘油醚的双官能试剂连接到氨基取代的阿魏酸或补骨脂素或溴化乙锭，其又与光化学连接至核酸 酸。 所得到的固定化核酸探针能够与互补核酸片段杂交，因此可用于诊断测定。
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公开(公告)号：USRE38960E1
公开(公告)日：2006-01-31
申请号：US10165281
申请日：2002-06-07
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2537/1376 , C12Q2527/101
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
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公开(公告)号：US06620586B2
公开(公告)日：2003-09-16
申请号：US09791030
申请日：2001-02-20
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q2565/501 , C12Q2537/143 , C12Q2523/319
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the invention provides for methods and combinations for analyzing nucleic acids in a plurality of samples using a plurality of detectably different signature labels and a probe that is hybridizable to each of the target nucleic acids. The invention also provides for a method for quantifying a nucleic acid by analyzing the amount of a label, e.g., a photoactivatable label, attached to the target nucleic acid.
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公开(公告)号：US06379930B1
公开(公告)日：2002-04-30
申请号：US09384717
申请日：1999-08-26
IPC分类号： C12P1934
CPC分类号： C12N15/1003 , C12Q1/6848 , C12Q2547/107 , C12Q2527/127 , C12Q2527/125
摘要： The present invention provides a cocktail of reagents for nucleic acid amplification that are stabilized by inclusion of a reversible inhibitor of undesirable reactions. Such cocktail of reagents eliminates the requirement for separate preparation and quality control of each reagent used in a reaction. Methods to prepare stabilized cocktails and to use stabilized cocktails also are included. The stabilized cocktail compositions also can include reagents to release nucleic acid from cells and to label the nucleic acid, allowing detection of nucleic acid in a sample with a single reagent addition step. The invention also provides kits for performing the above methods.
摘要翻译： 本发明提供用于核酸扩增的试剂的混合物，其通过包含不期望的反应的可逆抑制剂而被稳定化。 这种试剂混合物消除了对反应中使用的每种试剂的单独制备和质量控制的要求。 还包括制备稳定的鸡尾酒和使用稳定的鸡尾酒的方法。 稳定化的混合物组合物还可以包括从细胞中释放核酸并标记核酸的试剂，允许用单个试剂添加步骤检测样品中的核酸。 本发明还提供了用于执行上述方法的试剂盒。
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公开(公告)号：US06214587B1
公开(公告)日：2001-04-10
申请号：US09506282
申请日：2000-02-17
IPC分类号： C12P1934
CPC分类号： C12Q1/6844 , C12Q2565/537 , C12Q2537/1376 , C12Q2527/101
摘要： Methods for amplifying target nucleic acid sequences using a nucleic acid polymerase lacking 5′ exonuclease activity and a set of oligonucleotide primers. Preferably, a primer array is used. The primer array contains two sets of primers. One set contains at least two complementary primers. The other set contains at least two sense primers. Using the described methods amplification can be carried out under essentially constant environmental conditions without the requirement for exonuclease activity or restriction endonuclease activity.
摘要翻译： 使用缺少5'核酸外切酶活性的核酸聚合酶和一组寡核苷酸引物扩增靶核酸序列的方法。 优选使用引物数组。 引物数组包含两组引物。 一组含有至少两个互补引物。 另一组包含至少两个有义引物。 使用所述方法可以在基本恒定的环境条件下进行扩增，而不需要外切核酸酶活性或限制性内切核酸酶活性。
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30.发明授权Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides 失效限制性内切核酸酶序列用于切割硫代磷酸酯寡核苷酸
公开(公告)号：US5652126A
公开(公告)日：1997-07-29
申请号：US484816
申请日：1995-06-07
摘要： The present invention relates to methods of cleaving phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences can be used to facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.
摘要翻译： 本发明涉及切割硫代磷酸酯寡核苷酸的方法。 特别地，它涉及使用某些限制性内切酶来切割含有限制性内切核酸酶识别序列的硫代磷酸酯寡核苷酸。 这些限制性序列可用于促进相对切割抗性的硫代磷酸酯寡核苷酸的切割，从而促进其在合成后的分离和纯化。
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