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    METHOD FOR INCREASING N-GLYCOSYLATION SITE OCCUPANCY ON THERAPEUTIC GLYCOPROTEINS PRODUCED IN PICHIA PASTORIS
    26.
    发明申请
    METHOD FOR INCREASING N-GLYCOSYLATION SITE OCCUPANCY ON THERAPEUTIC GLYCOPROTEINS PRODUCED IN PICHIA PASTORIS 有权
    在PICHIA PASTORIS生产的治疗性GLYCOPROTEIN上增加N-糖苷化位点的方法

    公开(公告)号：US20120328626A1

    公开(公告)日：2012-12-27

    申请号：US13579972

    申请日：2011-02-23

    申请人： Natarajan Sethuraman ,  Byung-Kwon Choi ,  Bianka Prinz ,  Michael Meehl ,  Terrance Stadheim

    发明人： Natarajan Sethuraman ,  Byung-Kwon Choi ,  Bianka Prinz ,  Michael Meehl ,  Terrance Stadheim

    IPC分类号： C12P21/02 ,  A61K39/395 ,  A61P31/14 ,  C12N1/15 ,  C12N5/10 ,  A61K39/42 ,  C12N1/19

    CPC分类号： C07K16/2863 ,  C07K14/44 ,  C07K16/1027 ,  C07K16/32 ,  C07K2317/14 ,  C07K2317/21 ,  C07K2317/41 ,  C12N15/81 ,  C12P21/005

    摘要： Described is a method for increasing the N-glycosylation site occupancy of a therapeutic glycoprotein produced in recombinant host cells modified as described herein and genetically engineered to express the glycoprotein compared to the N-glycosylation site occupancy of the therapeutic glycoprotein produced in a recombinant host cell not modified as described herein. In particular, the method provides recombinant host cells that overexpress a heterologous single-subunit oligosaccharyltransferase, which in particular embodiments is capable of functionally suppressing the lethal phenotype of a mutation of at least one essential protein of the yeast oligosaccharyltransferase (OTase) complex, for example, the Leishmania major STT3D protein, in the presence of expression of the host cell genes encoding the endogenous OTase complex. The method is useful for both producing therapeutic glycoproteins with increased N-glycosylation site occupancy in lower eukaryote cells such as yeast and filamentous fungi and in higher eukaryote cells such as plant and insect cells and mammalian cells.

    摘要翻译： 描述了增加如本文所述修饰的重组宿主细胞中产生的治疗性糖蛋白的N-糖基化位点占有率的方法，并且与在重组宿主细胞中产生的治疗性糖蛋白的N-糖基化位点占据相比，进行遗传工程改造以表达糖蛋白 未经本文所述修饰。 特别地，该方法提供过表达异源单亚基寡糖基转移酶的重组宿主细胞，其在特定实施方案中能够功能性地抑制酵母寡糖转移酶（OTase）复合物的至少一种必需蛋白质的突变的致死表型，例如 ，利什曼原虫主要STT3D蛋白，在表达编码内源性OTase复合物的宿主细胞基因的存在下。 该方法对于在低等真核生物细胞如酵母和丝状真菌以及高等真核细胞如植物和昆虫细胞和哺乳动物细胞中产生具有增加的N-糖基化位点占有的治疗性糖蛋白是有用的。

    VECTORS AND YEAST STRAINS FOR PROTEIN PRODUCTION: CA2+ ATPASE OVEREXPRESSION
    27.
    发明申请
    VECTORS AND YEAST STRAINS FOR PROTEIN PRODUCTION: CA2+ ATPASE OVEREXPRESSION 失效
    蛋白质生产的载体和YEAST菌株：CA2 + ATPase过表达

    公开(公告)号：US20110143396A1

    公开(公告)日：2011-06-16

    申请号：US13057807

    申请日：2009-08-10

    申请人： Byung-Kwon Choi

    发明人： Byung-Kwon Choi

    IPC分类号： C12P21/00 ,  C12N1/15 ,  C12N1/19

    CPC分类号： C07K16/00 ,  C12N15/815 ,  C12P21/005

    摘要： Lower eukaryote host cells in which an endogenous or heterologous Ca2+ ATPase is overexpressed are described. Also described are lower eukaryote host cells in which a calreticulin and/or ERp57 protein are overexpressed. These host cells are useful for producing recombinant glycoproteins that have reduced O-glycosylation.

    摘要翻译： 描述了内源或异源Ca2 + ATP酶过表达的低等真核生物宿主细胞。 还描述了其中钙网蛋白和/或ERp57蛋白过表达的低等真核细胞宿主细胞。 这些宿主细胞可用于产生具有降低的O-糖基化的重组糖蛋白。

    Network-based robot control system and robot velocity control method in the network-based robot control system
    28.
    发明授权
    Network-based robot control system and robot velocity control method in the network-based robot control system 有权
    基于网络的机器人控制系统和机器人速度控制方法在基于网络的机器人控制系统中

    公开(公告)号：US07890210B2

    公开(公告)日：2011-02-15

    申请号：US11395768

    申请日：2006-03-31

    申请人： Byung-Kwon Choi ,  Hyun-Sik Shim ,  Joon-Koo Kim

    发明人： Byung-Kwon Choi ,  Hyun-Sik Shim ,  Joon-Koo Kim

    IPC分类号： G06F19/00

    CPC分类号： B25J9/1689 ,  G05B2219/40174

    摘要： Provided are a network-based robot control system and a robot velocity control method in the network-based robot control system. A client calculates a robot control velocity according to its reception state of video data frames captured by a robot, generates a robot control message including the calculated robot control velocity, and transmits the robot control message to the robot. The robot then changes its velocity according to the robot control velocity included in the received robot control message. In this way, the velocity of the robot is controlled according to the video data reception state of the client, thereby allowing a user to easily control the robot regardless of the performance of the client.

    摘要翻译： 提供了一种基于网络的机器人控制系统和基于网络的机器人控制系统中的机器人速度控制方法。 客户端根据机器人捕获的视频数据帧的接收状态来计算机器人控制速度，生成包含所计算的机器人控制速度的机器人控制消息，并将机器人控制消息发送给机器人。 然后机器人根据包含在接收的机器人控制消息中的机器人控制速度改变其速度。 以这种方式，根据客户端的视频数据接收状态来控制机器人的速度，从而允许用户容易地控制机器人，而不管客户端的性能如何。

    SOFTWARE ROBOT APPARATUS
    29.
    发明申请
    SOFTWARE ROBOT APPARATUS 有权
    软件机器人装置

    公开(公告)号：US20080066065A1

    公开(公告)日：2008-03-13

    申请号：US11852047

    申请日：2007-09-07

    申请人： Joon Koo Kim ,  Kwang-Choon Kim ,  Byung-Kwon Choi

    发明人： Joon Koo Kim ,  Kwang-Choon Kim ,  Byung-Kwon Choi

    IPC分类号： G06F9/44

    CPC分类号： G06N3/004 ,  G06F8/65 ,  G06N3/126

    摘要： A software robot apparatus (sobot apparatus) with a genetic code is disclosed. The sobot apparatus includes an application module, an artificial animated object engine, a sobot protocol module, an update agent, an update protocol module, a network interface, a sobot player, a script engine, a player database, a repository interface, a sobot graphic/audio interface, a 3D engine, a system software, a memory device, a display part, and an audio output part.

    摘要翻译： 公开了具有遗传密码的软件机器人装置（sobot装置）。 sobot装置包括应用模块，人造动画对象引擎，sobot协议模块，更新代理，更新协议模块，网络接口，sobot玩家，脚本引擎，玩家数据库，存储库界面，sobot 图形/音频接口，3D引擎，系统软件，存储设备，显示部分和音频输出部分。