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21.发明授权公开(公告)号：US08093020B2
公开(公告)日：2012-01-10
申请号：US12343233
申请日：2008-12-23
申请人： Eric B. Shain
发明人： Eric B. Shain
IPC分类号： C12P19/34
CPC分类号： G06F19/22 , C12Q1/6858 , G06F19/24 , C12Q2537/165
摘要： In various embodiments this invention provides novel methods for discriminating two or more different target nucleic acids. In certain embodiments the methods comprise providing data amplification reactions comprising reagents to amplify two or more different target nucleic acids where the data comprise signals comprising an amplitude measurement representing the degree of amplification of each target nucleic acid in the amplification reaction and the time point in the amplification reaction at which the amplitude is measured; determining an efficiency related transform of the data, determining an efficiency related value for each target nucleic acid that is the maximum magnitude of the efficiency related transform; and outputting the efficiency related values in the amplification reaction for each target nucleic acid, where the relative amplitudes of the efficiency related values for each target nucleic acid is an indicator of the presence of each of said nucleic acids in said sample.
摘要翻译： 在各种实施方案中，本发明提供用于区分两种或更多种不同靶核酸的新方法。 在某些实施方案中，所述方法包括提供包含用于扩增两种或更多种不同靶核酸的试剂的数据扩增反应，其中所述数据包括包括表示扩增反应中每个靶核酸的扩增程度的振幅测量的信号，以及 测量振幅的扩增反应; 确定所述数据的效率相关变换，确定作为所述效率相关变换的最大幅度的每个靶核酸的效率相关值; 并输出针对每个靶核酸的扩增反应中的效率相关值，其中每个靶核酸的效率相关值的相对幅度是所述样品中每种所述核酸的存在的指标。
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公开(公告)号：US07565250B2
公开(公告)日：2009-07-21
申请号：US10991025
申请日：2004-11-17
CPC分类号： G06F19/22 , C12Q1/68 , C12Q1/6851 , C12Q1/686 , C12Q2561/113 , C12Q2545/101 , C12Q2537/165
摘要： A method and system for determining the quantity of an analyte initially present in a chemical and or biological reaction as well as a computer implemented method and system to automate portions of the analysis comprising mathematical or graphical analysis of an amplification reaction.
摘要翻译： 用于确定最初存在于化学和/或生物反应中的分析物的量的方法和系统以及计算机实现的方法和系统，以使包括扩增反应的数学或图形分析的部分分析自动化。
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公开(公告)号：US06426045B1
公开(公告)日：2002-07-30
申请号：US09407716
申请日：1999-09-28
IPC分类号： G01N2141
CPC分类号： G01N21/4133 , G01N21/05 , G01N21/274 , Y10T436/144444 , Y10T436/146666 , Y10T436/147777 , Y10T436/171538 , Y10T436/200833
摘要： Apparatus and method for determining at least one parameter, e. g., concentration, of at least one analyte, e. g., urea, of a biological sample, e. g., urine. A biological sample particularly suitable for the apparatus and method of this invention is urine. In general, spectroscopic measurements can be used to quantify the concentrations of one or more analytes in a biological sample. In order to obtain concentration values of certain analytes, such as hemoglobin and bilirubin, visible light absorption spectroscopy can be used. In order to obtain concentration values of other analytes, such as urea, creatinine, glucose, ketones, and protein, infrared light absorption spectroscopy can be used. The apparatus and method of this invention utilize one or more mathematical techniques to improve the accuracy of measurement of parameters of analytes in a biological sample. The invention also provides an apparatus and method for measuring the refractive index of a sample of biological fluid while making spectroscopic measurements substantially simultaneously.
摘要翻译： 用于确定至少一个参数的装置和方法， 至少一种分析物的浓度，例如， 例如生物样品的尿素，例如， 例如尿。 特别适用于本发明的装置和方法的生物样品是尿液。 通常，光谱测量可用于量化生物样品中一种或多种分析物的浓度。 为了获得某些分析物（如血红蛋白和胆红素）的浓度值，可以使用可见光吸收光谱。 为了获得尿素，肌酐，葡萄糖，酮和蛋白质等其他分析物的浓度值，可以使用红外光吸收光谱。 本发明的装置和方法利用一种或多种数学技术来提高生物样品中分析物参数的测量精度。 本发明还提供了一种用于在基本上同时进行光谱测量的同时测量生物流体样品的折射率的装置和方法。
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公开(公告)号：US20090314802A1
公开(公告)日：2009-12-24
申请号：US12486215
申请日：2009-06-17
申请人： Scott Safar , Stephen L. Herchenbach , Chadwick M. Dunn , Charles Michael Galitz , Julius J. Toth , Ronald E. Kukla , Michael G. Lowery , Eric B. Shain
发明人： Scott Safar , Stephen L. Herchenbach , Chadwick M. Dunn , Charles Michael Galitz , Julius J. Toth , Ronald E. Kukla , Michael G. Lowery , Eric B. Shain
CPC分类号： G01N35/1004 , B01L3/50255 , B01L3/5082 , B01L99/00 , B01L2200/0668 , B01L2400/049 , B01L2400/0655 , B01L2400/0688 , G01N35/0098 , G01N35/1065 , Y10T137/0391 , Y10T137/0396 , Y10T436/25375 , Y10T436/2575
摘要： A reaction vessel with a bottom drain opening supporting a selected unpressured head of fluid by the surface tension of the fluid. A device processing zone includes a support for spaced rows of reaction vessels, passages communicating with their drain openings of supported vessels, and a pressure source for selectively draining fluid through the drain openings. Generally horizontal bar magnets are supported for selected vertical movement between the vessel rows. A dispensing head has X discharge openings selectively positionable over X selected reaction vessels. A metering pump mechanism selectively meters X a selected quantity of fluid a bulk supply (where X is at least four), and selectively pumps the metered selected quantities through the drain openings to the selected reaction vessels. Methods of drawing fluid from the vessels using the pressure source, and moving the magnets to form a pellet of analyte are also included.
摘要翻译： 具有底部排放开口的反应容器，其通过流体的表面张力支撑选定的未压缩流体的头部。 装置处理区域包括支撑隔离排列的反应容器，与其支撑的容器的排放开口连通的通道，以及用于选择性地通过排放开口排出流体的压力源。 通常，水平条形磁体被支撑用于在容器列之间选择的垂直运动。 分配头具有可选择性地定位在X选择的反应容器上的X个排出口。 计量泵机构选择性地测量选定量的流体的批量供应（其中X是至少四个），并且选择性地将经计量的所选量通过排放开口泵送到选定的反应容器。 还包括使用压力源从容器中抽取流体并移动磁体以形成分析物颗粒的方法。
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公开(公告)号：US20090252652A1
公开(公告)日：2009-10-08
申请号：US12486074
申请日：2009-06-17
申请人： Scott Safar , Stephen Herchenbach , Chadwick M. Dunn , Charles Michael Galitz , Julius J. Toth , Ronald E. Kukla , Michael G. Lowery , Eric B. Shain
发明人： Scott Safar , Stephen Herchenbach , Chadwick M. Dunn , Charles Michael Galitz , Julius J. Toth , Ronald E. Kukla , Michael G. Lowery , Eric B. Shain
IPC分类号： B01L3/00
CPC分类号： G01N35/1004 , B01L3/50255 , B01L3/5082 , B01L99/00 , B01L2200/0668 , B01L2400/049 , B01L2400/0655 , B01L2400/0688 , G01N35/0098 , G01N35/1065 , Y10T137/0391 , Y10T137/0396 , Y10T436/25375 , Y10T436/2575
摘要： A reaction vessel with a bottom drain opening supporting a selected unpressured head of fluid by the surface tension of the fluid. A device processing zone includes a support for spaced rows of reaction vessels, passages communicating with their drain openings of supported vessels, and a pressure source for selectively draining fluid through the drain openings. Generally horizontal bar magnets are supported for selected vertical movement between the vessel rows. A dispensing head has X discharge openings selectively positionable over X selected reaction vessels. A metering pump mechanism selectively meters X a selected quantity of fluid a bulk supply (where X is at least four), and selectively pumps the metered selected quantities through the drain openings to the selected reaction vessels. Methods of drawing fluid from the vessels using the pressure source, and moving the magnets to form a pellet of analyte are also included.
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26.发明授权Method for optical measurements of tissue to determine disease state or concentration of an analyte 有权用于光学测量组织以确定分析物的疾病状态或浓度的方法公开(公告)号：US07167734B2
公开(公告)日：2007-01-23
申请号：US09834440
申请日：2001-04-13
IPC分类号： A61B5/00
CPC分类号： A61B5/1495 , A61B5/14532 , A61B5/1455 , A61B5/7264
摘要： A method for collecting optical data at two morphologically similar, substantially non-overlapping, and preferably adjacent, areas on the surface of a tissue, while the temperature in each area is being maintained or modulated according to a temperature program. The optical data obtained are inserted into a mathematical relationship, e.g., an algorithm, that can be used to predict a disease state (such as the diabetes mellitus disease state) or the concentration of an analyte for indicating a physical condition (such as blood glucose level). This invention can be used to differentiate between disease status, such as, for example, diabetic and non-diabetic. The method involves the generation of a calibration (or training) set that utilizes the relationship between optical signals emanating from the skin under different thermal stimuli and disease status, e.g., diabetic status, established clinically. This calibration set can be used to predict the disease state of other subjects. Structural changes, as well as circulatory changes, due to a disease state are determined at two morphologically similar, but substantially non-overlapping areas on the surface of human tissue, e.g., the skin of a forearm, with each area being subjected to different temperature modulation programs. In addition to determination of a disease state, this invention can also be used to determine the concentration of an analyte in the tissues. This invention also provides an apparatus for the determination of a disease state, such as diabetes, or concentration of an analyte, such as blood glucose level, by the method of this invention.
摘要翻译： 一种用于在组织表面上的两个形态上类似的，基本上不重叠的，优选相邻的区域收集光学数据的方法，同时根据温度程序维持或调节每个区域中的温度。 所获得的光学数据被插入到数学关系中，例如算法，其可用于预测疾病状态（例如糖尿病状态）或用于指示身体状况的分析物的浓度（例如血糖 水平）。 本发明可用于区分疾病状况，例如糖尿病和非糖尿病。 该方法包括产生校准（或训练）组，其利用在不同热刺激下发生的光学信号与疾病状态（例如临床确立的糖尿病状态）之间的关系。 该校准集可用于预测其他受试者的疾病状态。 由于疾病状态引起的结构变化以及循环变化在人组织表面上的两个形态相似但基本上非重叠的区域确定，例如前臂的皮肤，每个区域经受不同的温度 调制程序。 除了确定疾病状态之外，本发明还可以用于确定组织中分析物的浓度。 本发明还提供了一种用于通过本发明的方法测定疾病状态的装置，例如糖尿病或分析物的浓度，例如血糖水平。
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公开(公告)号：US06773922B2
公开(公告)日：2004-08-10
申请号：US10191148
申请日：2002-07-09
IPC分类号： G01N2141
CPC分类号： G01N21/4133 , G01N21/05 , G01N21/274 , Y10T436/144444 , Y10T436/146666 , Y10T436/147777 , Y10T436/171538 , Y10T436/200833
摘要： Apparatus and method for determining at least one parameter, e.g., concentration, of at least one analyte, e.g., urea, of a biological sample, e.g., urine. A biological sample particularly suitable for the apparatus and method of this invention is urine. In general, spectroscopic measurements can be used to quantify the concentrations of one or more analytes in a biological sample. In order to obtain concentration values of certain analytes, such as hemoglobin and bilirubin, visible light absorption spectroscopy can be used. In order to obtain concentration values of other analytes, such as urea, creatinine, glucose, ketones, and protein, infrared light absorption spectroscopy can be used. The apparatus and method of this invention utilize one or more mathematical techniques to improve the accuracy of measurement of parameters of analytes in a biological sample. The invention also provides an apparatus and method for measuring the refractive index of a sample of biological fluid while making spectroscopic measurements substantially simultaneously.
摘要翻译： 用于确定生物样品例如尿液的至少一种分析物（例如尿素）的至少一个参数例如浓度的装置和方法。 特别适用于本发明的装置和方法的生物样品是尿液。 通常，光谱测量可用于量化生物样品中一种或多种分析物的浓度。 为了获得某些分析物（如血红蛋白和胆红素）的浓度值，可以使用可见光吸收光谱。 为了获得其他分析物（如尿素，肌酐，葡萄糖，酮和蛋白质）的浓度值，可以使用红外光吸收光谱。 本发明的装置和方法利用一种或多种数学技术来提高生物样品中分析物参数的测量精度。 本发明还提供了一种用于在基本上同时进行光谱测量的同时测量生物流体样品的折射率的装置和方法。
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公开(公告)号：US08409528B2
公开(公告)日：2013-04-02
申请号：US10865207
申请日：2004-06-10
申请人： Scott Safar , Stephen Herchenbach , Chadwick M. Dunn , Charles Michael Galitz , Julius J. Toth , Ronald E. Kukla , Michael G. Lowery , Eric B. Shain
发明人： Scott Safar , Stephen Herchenbach , Chadwick M. Dunn , Charles Michael Galitz , Julius J. Toth , Ronald E. Kukla , Michael G. Lowery , Eric B. Shain
IPC分类号： B01L3/00
CPC分类号： G01N35/1004 , B01L3/50255 , B01L3/5082 , B01L99/00 , B01L2200/0668 , B01L2400/049 , B01L2400/0655 , B01L2400/0688 , G01N35/0098 , G01N35/1065 , Y10T137/0391 , Y10T137/0396 , Y10T436/25375 , Y10T436/2575
摘要： A reaction vessel with a bottom drain opening supporting a selected unpressured head of fluid by the surface tension of the fluid. A device processing zone includes a support for spaced rows of reaction vessels, passages communicating with their drain openings of supported vessels, and a pressure source for selectively draining fluid through the drain openings. Generally horizontal bar magnets are supported for selected vertical movement between the vessel rows. A dispensing head has X discharge openings selectively positionable over X selected reaction vessels. A metering pump mechanism selectively meters X a selected quantity of fluid a bulk supply (where X is at least four), and selectively pumps the metered selected quantities through the drain openings to the selected reaction vessels. Methods of drawing fluid from the vessels using the pressure source, and moving the magnets to form a pellet of analyte are also included.
摘要翻译： 具有底部排放开口的反应容器，其通过流体的表面张力支撑选定的未压缩流体的头部。 装置处理区域包括支撑隔离排列的反应容器，与其支撑的容器的排放开口连通的通道，以及用于选择性地通过排放开口排出流体的压力源。 通常，水平条形磁体被支撑用于在容器列之间选择的垂直运动。 分配头具有可选择性地定位在X选择的反应容器上的X个排出口。 计量泵机构选择性地将X选定量的流体计量为批量供应（其中X为至少四个），并且选择性地将计量的所选量通过排放开口泵送到选定的反应容器。 还包括使用压力源从容器中抽取流体并移动磁体以形成分析物颗粒的方法。
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29.发明授权Image based correction for unwanted light signals in a specific region of interest 有权基于特定感兴趣区域的不需要的光信号的基于图像的校正公开(公告)号：US08249381B2
公开(公告)日：2012-08-21
申请号：US12707011
申请日：2010-02-17
CPC分类号： G06T7/11 , G06F19/20 , G06T2207/30072
摘要： A method for correcting the signal in an image having a plurality of regions of interest, the method comprising the steps of: (a) providing an image having a plurality of regions of interest, these regions of interest having areas between them; (b) determining a region of correction between at least two regions of interest; (c) calculating a correction signal from the region of correction; and (d) using the correction signal to correct a measured signal from one or more regions of interest. This invention also provides a method for defining a region of correction for use in a method for correcting the signal in an image having a plurality of regions of interest, the defining method comprising the steps of: (a) providing an image having a plurality of regions of interest; (b) extracting geometric information for a plurality of regions of interest; (c) selecting a location between at least two regions of interest; (d) selecting at least one parameter to describe regions of correction; and (e) constructing regions of correction between the at least two regions of interest.
摘要翻译： 一种用于校正具有多个感兴趣区域的图像中的信号的方法，所述方法包括以下步骤：（a）提供具有多个感兴趣区域的图像，所述感兴趣区域在它们之间具有区域; （b）确定至少两个感兴趣区域之间的校正区域; （c）从校正区域计算校正信号; 和（d）使用校正信号来校正来自一个或多个感兴趣区域的测量信号。 本发明还提供了一种用于定义用于校正具有多个感兴趣区域的图像中的信号的方法中的校正区域的方法，所述定义方法包括以下步骤：（a）提供具有多个 感兴趣的区域 （b）提取多个感兴趣区域的几何信息; （c）选择至少两个感兴趣的区域之间的位置; （d）选择至少一个参数来描述校正区域; 和（e）构建所述至少两个感兴趣区域之间的校正区域。
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公开(公告)号：US20120164652A1
公开(公告)日：2012-06-28
申请号：US13337784
申请日：2011-12-27
申请人： John M. Clemens , Eric B. Shain
发明人： John M. Clemens , Eric B. Shain
CPC分类号： C12Q1/686 , C12Q1/6851 , G01N21/6428 , G01N2021/6441 , C12Q2545/107 , C12Q2563/159
摘要： The present invention provides systems, devices, methods, kits, and compositions for nucleic acid analysis using digital PCR. In particular, methods are provided to analyze high titer samples that cannot be divided into a sufficient number of partitions containing zero nucleic acid molecules per partition to allow for Poisson analysis (digital PCR analysis).
摘要翻译： 本发明提供使用数字PCR进行核酸分析的系统，装置，方法，试剂盒和组合物。 特别地，提供了分析高滴度样品的方法，其不能被分成足够数量的每个分区含有零个核酸分子的分区以允许泊松分析（数字PCR分析）。
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