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65 条记录 (耗时 0.034 秒)

    Mutant carotenoid ketolases
    21.
    发明申请
    Mutant carotenoid ketolases 失效
    突变类胡萝卜素酮醇酶

    公开(公告)号：US20070238149A1

    公开(公告)日：2007-10-11

    申请号：US11393082

    申请日：2006-03-30

    申请人： Qiong Cheng ,  Luan Tao

    发明人： Qiong Cheng ,  Luan Tao

    IPC分类号： C12P23/00 ,  C12P21/06 ,  C07H21/04 ,  C12N9/02 ,  C12N1/21 ,  C12N1/18 ,  C12N15/74

    CPC分类号： C12N9/0069 ,  C12P23/00

    摘要： CrtW carotenoid ketolases are provided that are useful for the production of astaxanthin and other cyclic ketocarotenoids. The mutant ketolase genes of the present invention encode polypeptides characterized by an improvement in astaxanthin synthesis activity when converting cyclic hydroxylated carotenoid intermediates into astaxanthin. Expression of the mutant carotenoid ketolases in heterologous hosts enabled increased production of astaxanthin relative to the Sphingomonas melonis DC18 CrtW.

    摘要翻译： 提供了可用于生产虾青素和其他环状酮类胡萝卜素的CrtW类胡萝卜素酮酶。 本发明的突变型酮糖酶基因编码多肽，其特征在于将环状羟基化类胡萝卜素中间体转化成虾青素时，虾青素合成活性得到改善。 突变型类胡萝卜素酮酶在异源宿主中的表达使得虾青素相对于甜菜根鞘节DC18 CrtW的产量增加。

    Carotenoid ketolase gene
    23.
    发明授权
    Carotenoid ketolase gene 失效

    公开(公告)号：US06984523B2

    公开(公告)日：2006-01-10

    申请号：US10209372

    申请日：2002-07-30

    申请人： Qiong Cheng ,  Luan Tao

    发明人： Qiong Cheng ,  Luan Tao

    IPC分类号： C12N15/54

    CPC分类号： C12N9/0069 ,  C12P23/00 ,  C12Y114/15802

    摘要： A ketolase gene has been isolated from Rhodococcus erythropolis AN12 strain encoding a carotenoid modification enzyme of the carotenoid biosynthetic pathway. The gene and gene product are the first isolated from a Rhodococcus strain. Six conserved amino acid motifs have been identified as the characteristic of this type of ketolase enzymes. The gene and gene product of the present invention may be used in a variety of ways for the production of keto-carotenoid compounds in a variety of organisms.

    Method for synthesis of aryl-carotenoids
    24.
    发明申请
    Method for synthesis of aryl-carotenoids 失效
    合成芳香类胡萝卜素的方法

    公开(公告)号：US20050214896A1

    公开(公告)日：2005-09-29

    申请号：US10430129

    申请日：2003-05-05

    申请人： Qiong Cheng ,  Luan Tao ,  Pierre Rouvier

    发明人： Qiong Cheng ,  Luan Tao ,  Pierre Rouvier

    IPC分类号： A01H1/00 ,  C12N1/18 ,  C12N1/21 ,  C12N5/04 ,  C12N9/02 ,  C12P23/00

    CPC分类号： C12N9/0083 ,  C12P23/00

    摘要： A method for the production of aryl-carotenoids is provided through bioconversion of cyclic carotenoids having at least one β-ionone ring. Expression of a heterologous gene encoding a carotene desaturase (crtU) enzyme in a host cell that produces a suitable cyclic carotenoid substrate effect the production of aryl carotenoids.

    摘要翻译： 通过具有至少一个β-紫罗兰酮环的环状类胡萝卜素的生物转化提供了生产芳基类胡萝卜素的方法。 编码胡萝卜素去饱和酶（crtU）酶的异源基因在产生合适的环状类胡萝卜素底物的宿主细胞中的表达影响芳香类胡萝卜素的产生。

    Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number
    25.
    发明申请
    Broad host range pBBR1-based plasmid mutant derivatives having altered plasmid copy number 失效
    具有改变的质粒拷贝数的基于pBBR1的质粒突变体衍生物

    公开(公告)号：US20050130307A1

    公开(公告)日：2005-06-16

    申请号：US10940052

    申请日：2004-09-14

    申请人： Qiong Cheng ,  Luan Tao

    发明人： Qiong Cheng ,  Luan Tao

    IPC分类号： C12N20060101 ,  C12N1/21 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74

    CPC分类号： C12N15/70 ,  C12N15/74

    摘要： The present invention describes a mutant plasmid replication control region having the ability to convey a phenotype of altered plasmid copy number to the plasmid on which it resides. The mutant replication control region is based on a similar region isolated from the pBBR1 plasmid family. Plasmids containing this replication control region cannot be classed as belonging to any known incompatibility group and thus may co-exist with a broad range of other plasmids in a single host.

    摘要翻译： 本发明描述了具有将改变的质粒拷贝数表型转移到其所在质粒上的突变质粒复制控制区。 突变体复制控制区基于与pBBR1质粒家族分离的相似区域。 含有该复制控制区的质粒不能归类为任何已知的不相容性基团，因此可以与单个宿主中的宽范围的其他质粒共存。

    Genes encoding carotenoid compounds
    28.
    发明申请
    Genes encoding carotenoid compounds 失效
    编码类胡萝卜素的基因

    公开(公告)号：US20050014219A1

    公开(公告)日：2005-01-20

    申请号：US10810733

    申请日：2004-03-26

    申请人： Qiong Cheng ,  Luan Tao ,  Natalia Sedkova

    发明人： Qiong Cheng ,  Luan Tao ,  Natalia Sedkova

    IPC分类号： C07H21/04 ,  C12N1/21 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N9/90 ,  C12N15/52 ,  C12N15/74 ,  C12P7/26 ,  C12P23/00

    CPC分类号： C12N9/001 ,  C07H21/04 ,  C12N9/00 ,  C12N9/0004 ,  C12N9/0071 ,  C12N9/0083 ,  C12N9/1051 ,  C12N9/1085 ,  C12N9/90 ,  C12N15/52 ,  C12P23/00 ,  C12Y205/01029 ,  Y02E50/343

    摘要： A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crt)(-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (Crtl), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.

    摘要翻译： 从Panteoa stewartii菌株DC413中分离出一种致病基因生物合成基因簇，其中簇的遗传组织为crtE-idi-crt（-crtY-crtI-crtB-crtZ），该簇内包含的基因编码geranylgeranyl pyrophosphate（GGPP） 合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），玉米黄质葡萄糖基转移酶（CrtX），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（Crtl），八氢番茄红素合酶（CrtB）和β-胡萝卜素羟化酶（CrtZ） 公开了基因及其产物可用于将焦磷酸法呢基转化为类胡萝卜素。含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

    Mutant carotenoid ketolases
    29.
    发明授权
    Mutant carotenoid ketolases 失效
    突变类胡萝卜素酮醇酶

    公开(公告)号：US07393671B2

    公开(公告)日：2008-07-01

    申请号：US11393082

    申请日：2006-03-30

    申请人： Qiong Cheng ,  Luan Tao

    发明人： Qiong Cheng ,  Luan Tao

    IPC分类号： C12P23/00 ,  C12N5/10 ,  C12N1/21 ,  C12N15/00 ,  C12N5/14 ,  C12N1/12 ,  C12N1/11 ,  C12N1/13 ,  C12N1/15 ,  C12N1/19 ,  C07H21/00 ,  C12N9/02 ,  C12P21/00 ,  C07K14/00

    CPC分类号： C12N9/0069 ,  C12P23/00

    摘要： CrtW carotenoid ketolases are provided that are useful for the production of astaxanthin and other cyclic ketocarotenoids. The mutant ketolase genes of the present invention encode polypeptides characterized by an improvement in astaxanthin synthesis activity when converting cyclic hydroxylated carotenoid intermediates into astaxanthin. Expression of the mutant carotenoid ketolases in heterologous hosts enabled increased production of astaxanthin relative to the Sphingomonas melonis DC18 CrtW.

    摘要翻译： 提供了可用于生产虾青素和其他环状酮类胡萝卜素的CrtW类胡萝卜素酮酶。 本发明的突变型酮糖酶基因编码多肽，其特征在于将环状羟基化类胡萝卜素中间体转化成虾青素时，虾青素合成活性得到改善。 突变型类胡萝卜素酮酶在异源宿主中的表达使得虾青素相对于甜菜根鞘节DC18 CrtW的产量增加。

    Mutations affecting carotenoid production
    30.
    发明授权
    Mutations affecting carotenoid production 失效
    影响类胡萝卜素生产的突变

    公开(公告)号：US07232665B2

    公开(公告)日：2007-06-19

    申请号：US10735008

    申请日：2003-12-12

    申请人： Qiong Cheng ,  Pierre E. Rouviere ,  Luan Tao

    发明人： Qiong Cheng ,  Pierre E. Rouviere ,  Luan Tao

    IPC分类号： C12P23/00 ,  C12N1/20

    CPC分类号： C12P23/00 ,  C07K14/24 ,  C12N15/52

    摘要： Mutations in genes having no direct relationship to the carotenoid biosynthetic pathway have been found to increase carbon flux through that pathway. Complete disruption in the deaD, mreC, and yfhE genes were effective. Additionally where genes of the lower carotenoid pathway reside on a plasmid having either a p15A or pMB1 replicon, mutations in the thrS, rspA, rpoC, yjeR, and rhoL were found effective.

    摘要翻译： 已经发现与类胡萝卜素生物合成途径没有直接关系的基因中的突变增加通过该途径的碳通量。 deaD，mreC和yfhE基因的完全破坏是有效的。 此外，较低类胡萝卜素途径的基因存在于具有p15A或pMB1复制子的质粒上，发现thrS，rspA，rpoC，yjeR和rhoL中的突变是有效的。