利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

35 条记录 (耗时 0.016 秒)

    Use of pectinolytic enzymes for the treatment of fruit and vegetable mash and enzyme sequences therefor
    21.
    发明授权
    Use of pectinolytic enzymes for the treatment of fruit and vegetable mash and enzyme sequences therefor 有权
    使用果胶分解酶处理水果和蔬菜糊状物和酶序列

    公开(公告)号：US08748149B2

    公开(公告)日：2014-06-10

    申请号：US12994075

    申请日：2009-05-22

    申请人： Terhi Puranen ,  Bernhard Seiboth ,  Klaudija Milos ,  Wilfried Theiss ,  Jarno Kallio ,  Christian Kubicek ,  Jari Vehmaanperä

    发明人： Terhi Puranen ,  Bernhard Seiboth ,  Klaudija Milos ,  Wilfried Theiss ,  Jarno Kallio ,  Christian Kubicek ,  Jari Vehmaanperä

    IPC分类号： C12N9/24 ,  C12N1/15 ,  C12N1/19

    CPC分类号： C12Y302/01015 ,  C12N9/2402 ,  C12Y302/01067

    摘要： The invention relates to the use of one or more pectinolytic enzyme(s) for the treatment of fruit or vegetable mash as well as a process for enzymatic treatment of fruit or vegetable mash comprising the step of adding one or more pectinolytic enzyme(s), wherein at least one pectinolytic enzyme is obtainable from Trichoderma reesei, as well as to a process for the preparation of a fruit or vegetable juice comprising the process for enzymatic treatment of fruit or vegetable mash. Moreover, the invention discloses recombinant DNA molecules encoding a polypeptide having endo-polygalacturonase activity, a polypeptide having exo-polygalacturonase activity, a polypeptide having exo-rhamnogalacturonase activity and a polypeptide having xylogalacturonase activity.

    摘要翻译： 本发明涉及一种或多种果胶分解酶用于处理水果或蔬菜醪液的用途以及用于酶或处理水果或蔬菜醪液的方法，包括添加一种或多种果胶分解酶， 其中至少一种果胶分解酶可从里氏木霉获得，以及一种用于制备水果或蔬菜汁的方法，其包括用于酶处理水果或蔬菜糊状物的方法。 此外，本发明公开了编码具有内切多聚半乳糖醛酸酶活性的多肽的重组DNA分子，具有外切多聚半乳糖醛酸酶活性的多肽，具有外分泌半乳糖醛酸聚糖酶活性的多肽和具有木糖聚半乳糖醛酸酶活性的多肽。

    Phospholipase polypeptide and a DNA encoding same
    22.
    发明授权
    Phospholipase polypeptide and a DNA encoding same 有权
    磷脂酶多肽和编码相同的DNA

    公开(公告)号：US08653241B2

    公开(公告)日：2014-02-18

    申请号：US13171005

    申请日：2011-06-28

    申请人： Khanh Q. Nguyen ,  Volker Marschner ,  Kornelia Titze ,  Bruno Winter

    发明人： Khanh Q. Nguyen ,  Volker Marschner ,  Kornelia Titze ,  Bruno Winter

    IPC分类号： C07K1/00

    CPC分类号： C12N9/18

    摘要： The invention relates to a DNA sequence that encodes a polypeptide with phospholipase activity and was isolated from Aspergillus and sequences derived therefrom, polypeptides with phospholipase activity encoded by these sequences as well as the use of these polypeptides for degumming of vegetable oil, for the preparation of dough and/or bakery products, for the preparation of dairy products, for processing steps in the textile industry and for related applications.

    摘要翻译： 本发明涉及一种编码具有磷脂酶活性的多肽的DNA序列，其从曲霉属中分离得到，由此得到的序列由这些序列编码的具有磷脂酶活性的多肽以及这些多肽用于植物油脱胶的用途，用于制备 面团和/或面包店产品，用于制备乳制品，用于纺织工业中的加工步骤和相关应用。

    Polypeptide having phytase activity and increased temperature resistance of the enzyme activity, and nucleotide sequence coding said polypeptide
    23.
    发明授权
    Polypeptide having phytase activity and increased temperature resistance of the enzyme activity, and nucleotide sequence coding said polypeptide 有权
    具有植酸酶活性和酶活性耐温度升高的多肽以及编码所述多肽的核苷酸序列

    公开(公告)号：US08420369B2

    公开(公告)日：2013-04-16

    申请号：US12312381

    申请日：2007-11-02

    申请人： Khanh Q. Nguyen ,  Bruno Winter

    发明人： Khanh Q. Nguyen ,  Bruno Winter

    IPC分类号： C12N9/16

    CPC分类号： C12N9/16 ,  A23K10/14 ,  A23L29/06 ,  C12Y301/03026

    摘要： The invention relates to a recombinant DNA molecule encoding a polypeptide having phytase activity and increased temperature stability and increased proteolytic stability of the enzyme activity. The DNA sequence has been obtained by variation of the mature wild-type E. coli phytase sequence with defined amino acid positions being modified in comparison to the wild-type sequence or with the sequences having N- and/or C-terminal extensions, respectively. The invention further relates to a method for expressing the recombinant phytase as well as its use in the food and animal feed technologies.

    摘要翻译： 本发明涉及编码具有植酸酶活性和增加的温度稳定性以及增加的酶活性的蛋白水解稳定性的多肽的重组DNA分子。 DNA序列是通过变异成熟的野生型大肠杆菌肌醇六磷酸酶序列得到的，其具有与野生型序列相比修饰的具有限定的氨基酸位置或与分别具有N-和/或C-末端延伸的序列 。 本发明还涉及用于表达重组植酸酶的方法及其在食品和动物饲料技术中的应用。

    Method for Recovering Oil from Plant Seeds
    24.
    发明申请
    Method for Recovering Oil from Plant Seeds 有权
    从植物种子中回收油的方法

    公开(公告)号：US20100041125A1

    公开(公告)日：2010-02-18

    申请号：US12448482

    申请日：2007-12-05

    申请人： Jörg Köhler ,  Volker Marschner ,  Bruno Winter

    发明人： Jörg Köhler ,  Volker Marschner ,  Bruno Winter

    IPC分类号： C12P1/00

    CPC分类号： C11B1/025 ,  Y02E50/13

    摘要： The invention relates to a method for recovering of oil from plant seeds characterized in that a) an aqueous solution containing one or more cellulolytic and/or lipolytic and/or pectinolytic and/or proteolytic enzyme(s) and/or phytase is sprayed onto the seed, b) the thus obtained seed is directly supplied to a one-stage or multistage pressing in a way known per se, optionally coupled to an extraction, and c) the oil is recovered in a way known per se and optionally further processed, and the use of the method, particularly in the production of edible oil or biodiesel.

    摘要翻译： 本发明涉及从植物种子中回收油的方法，其特征在于：a）将含有一种或多种纤维素分解和/或脂肪分解和/或果胶分解和/或蛋白水解酶和/或植酸酶的水溶液喷雾到 种子，b）将如此获得的种子以本身已知的方式直接供应至一级或多级压榨，任选地与提取物偶联，和c）以本身已知的方式回收油并任选进一步加工， 并采用该方法，特别是在生产食用油或生物柴油时。

    Cloning, expression and use of acid phospholipases
    28.
    发明授权
    Cloning, expression and use of acid phospholipases 有权

    公开(公告)号：US09322003B2

    公开(公告)日：2016-04-26

    申请号：US14692230

    申请日：2015-04-21

    申请人： AB Enzymes GMBH

    发明人： Khanh Quoc Nguyen ,  Kornelia Titze ,  Tatiana Schwarz ,  Silvia Paladino ,  Volker Marschner ,  Patrick Lorenz

    IPC分类号： C12N9/18 ,  A23D9/02 ,  A23D9/00 ,  A23J7/00 ,  A23K1/165 ,  C11B3/00 ,  C12N9/16

    CPC分类号： C12N9/18 ,  A23D9/00 ,  A23D9/02 ,  A23D9/04 ,  A23J7/00 ,  A23K20/189 ,  A23V2002/00 ,  C11B3/00 ,  C11B3/003 ,  C12N9/16 ,  C12Y301/01004 ,  C12Y301/01005 ,  C12Y301/01032

    摘要： The invention relates to a DNA sequence, which codes for a polypeptide having phospholipase activity essentially without lipase activity, characterized in that the DNA sequence is selected from a) DNA sequences that comprise a nucleotide sequence according to SEQ ID NO: 1, b) DNA sequences that comprise the coding sequence according to SEQ ID NO: 1, c) DNA sequences that code for the protein sequence according to SEQ ID NO: 2, d) DNA sequences that are coded for by the plasmid pPL3940-Topo2.5 with the restriction map according to FIG. 7, which is deposited under accession number DSM 22741, e) DNA sequences that hybridize under stringent conditions with one of the DNA sequences according to a), b), c) or d), f) DNA sequences that are related to the DNA sequences according to a), b), c), d) or e) due to the degeneration of the genetic code, and g) complementary strands to the sequences according to a) to f), wherein the DNA sequence is preferably derived from Aspergillus, and more preferably from Aspergillus fumigatus, and a polypeptide having phospholipase activity essentially without lipase activity selected from a) a polypeptide which is coded for by the coding part of a DNA sequence as defined above, b) a polypeptide having the sequence according to SEQ ID NO: 2 or a sequence derived therefrom, which may be obtained by substitution, addition, deletion of one or more amino acid(s), c) a polypeptide having a sequence that has at least 83% identity with the amino acids 1 to 299 of SEQ ID NO: 2, d) a polypeptide which is coded for by a nucleic acid sequence which hybridizes under stringent conditions with (i) nucleotides 55 to 1106 of SEQ ID NO: 1, (ii) the cDNA sequence contained in nucleotides 55 to 1106 of SEQ ID NO: 1, (iii) a partial sequence of (i) or (ii) composed of at least 100 nucleotides, or (iv) a complementary strand of (i), (ii) or (iii), e) a variant of the polypeptide having SEQ ID NO: 2, comprising a substitution, deletion and/or insertion of one or more amino acid(s), f) allelic variants to amino acid sequences a) to e).

    Expression system for the antibiotic-free production of polypeptides
    29.
    发明授权
    Expression system for the antibiotic-free production of polypeptides 有权
    用于无抗生素生产多肽的表达系统

    公开(公告)号：US08465946B2

    公开(公告)日：2013-06-18

    申请号：US12598712

    申请日：2008-02-12

    申请人： Céline Cadot ,  Tina Ploss ,  Ruth Schwerdtfeger ,  Bruno Winter

    发明人： Céline Cadot ,  Tina Ploss ,  Ruth Schwerdtfeger ,  Bruno Winter

    IPC分类号： C12P21/06 ,  C12N1/20

    CPC分类号： C12N15/75 ,  C12N9/0006

    摘要： The present invention relates to a microbial expression system for the production of polypeptides based on the use of extrachromosomal DNA, whereby no antibiotic marker genes for the selection of the host cell but DNA sequences that encode glycerine-3-phosphate dehydrogenase are used, and, thus, the production of the desired polypeptide, e.g., xylanase, does not need the addition of antibiotics. The expression system is free from antibiotic-resistance genes. The invention further relates to a DNA sequence that encodes a polypeptide with glycerine-3-phosphate dehydrogenase activity as well as a polypeptide with glycerine-3-phosphate dehydrogenase activity.

    摘要翻译： 本发明涉及一种用于根据染色体外DNA的使用生产多肽的微生物表达系统，由此不使用用于选择宿主细胞但编码甘油-3-磷酸脱氢酶的DNA序列的抗生素标记基因， 因此，所需多肽例如木聚糖酶的生产不需要添加抗生素。 表达系统不含抗生素抗性基因。 本发明还涉及编码具有甘油-3-磷酸脱氢酶活性的多肽的DNA序列以及具有甘油-3-磷酸脱氢酶活性的多肽。

    Polypeptide having phytase activity and nucleotide sequence encoding the same
    30.
    发明授权
    Polypeptide having phytase activity and nucleotide sequence encoding the same 有权
    具有植酸酶活性的多肽和编码其的核苷酸序列

    公开(公告)号：US08313937B2

    公开(公告)日：2012-11-20

    申请号：US11665631

    申请日：2005-10-14

    申请人： Khanh Q. Nguyen ,  Bruno Winter

    发明人： Khanh Q. Nguyen ,  Bruno Winter

    IPC分类号： C12N9/16 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12N9/16

    摘要： The present invention features a recombinant DNA molecule which, upon expression in a prokaryotic or eukaryotic host cell, encodes a polypeptide having phytase activity. In preferred embodiments, the recombinant DNA molecule comprises a DNA sequence selected from DNA sequences which have been obtained by variations of the mature wild-type E. coli phytase sequence, wherein at least one amino acid at position 200 or position 207 is mutated as compared to the wild-type sequence, where the recombinant DNA molecule is, upon expression in a suitable host cell, associated with an increased activity of the thus encoded protein in the culture supernatant.

    摘要翻译： 本发明的特征在于重组DNA分子，其在原核或真核宿主细胞中表达后，编码具有植酸酶活性的多肽。 在优选的实施方案中，重组DNA分子包含选自通过成熟野生型大肠杆菌植酸酶序列的变体获得的DNA序列的DNA序列，其中位置200或位置207处的至少一个氨基酸被突变 涉及在合适的宿主细胞中表达时与重组DNA分子相关的野生型序列，与培养上清液中如此编码的蛋白质的活性增加相关。