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    SYSTEM FOR EXPRESSION OF GENES IN PLANTS
    22.
    发明申请
    SYSTEM FOR EXPRESSION OF GENES IN PLANTS 有权

    公开(公告)号：US20170121721A1

    公开(公告)日：2017-05-04

    申请号：US15398388

    申请日：2017-01-04

    申请人： iBio, Inc.

    发明人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    IPC分类号： C12N15/82 ,  C12N7/00

    CPC分类号： C12N15/8203 ,  C07K14/61 ,  C07K14/62 ,  C12N7/00 ,  C12N15/8257 ,  C12N2770/00043 ,  C12P21/02

    摘要： The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

    SYSTEM FOR EXPRESSION OF GENES IN PLANTS
    27.
    发明申请
    SYSTEM FOR EXPRESSION OF GENES IN PLANTS 有权
    在植物中表达基因的系统

    公开(公告)号：US20140220674A1

    公开(公告)日：2014-08-07

    申请号：US14093833

    申请日：2013-12-02

    申请人： iBio, Inc.

    发明人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8203 ,  C07K14/61 ,  C07K14/62 ,  C12N7/00 ,  C12N15/8257 ,  C12N2770/00043 ,  C12P21/02

    摘要： The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

    摘要翻译： 本发明提供用于在植物中表达基因产物的反式互补系统。 通常，本发明提供了包括载体载体和生产者载体的系统，其都是基于植物病毒的。 生产者载体对于植物的成功的全身感染所需的至少一个功能是有缺陷的，例如复制，细胞到细胞运动或长距离运动。 载波向量以反向方式提供丢失的功能。 某些生产者载体缺乏功能外壳蛋白编码序列，在这种情况下，相应的生产者载体以反式提供外壳蛋白。 本发明还提供新的植物病毒载体和使用方法，例如在植物中产生多肽或活性RNA。

    Virus vector and use thereof
    28.
    发明授权
    Virus vector and use thereof 有权
    病毒载体及其用途

    公开(公告)号：US08586826B2

    公开(公告)日：2013-11-19

    申请号：US12450999

    申请日：2008-04-11

    申请人： Masashi Mori ,  Koji Dohi

    发明人： Masashi Mori ,  Koji Dohi

    IPC分类号： C07K14/005 ,  C12N15/67 ,  C12N15/40

    CPC分类号： C12N15/8203 ,  C12N15/8257 ,  C12N2770/00043

    摘要： It is intended to provide a polynucleotide comprising a viral base sequence, the viral base sequence containing: a first base sequence encoding a viral replication protein, and a second base sequence encoding a viral movement protein, the second base sequence being located downstream of the first base sequence and having a linking site for linking with an exogenous base sequence encoding a polypeptide to be expressed, the linking site being located downstream of the second base sequence, the second base sequence being obtained by modifying with a base sequence in a native sequence derived from a virus by insertion, substitution, or addition. By using this, a vector containing a viral base sequence is constructed, and a protein is efficiently produced without worsening growth of a host cell containing the vector.

    摘要翻译： 旨在提供包含病毒碱基序列的多核苷酸，所述病毒碱基序列包含编​​码病毒复制蛋白的第一碱基序列和编码病毒运动蛋白的第二碱基序列，所述第二碱基序列位于第一 碱基序列并具有与编码待表达多肽的外源碱基序列连接的连接位点，所述连接位点位于第二碱基序列的下游，第二碱基序列通过用天然序列衍生的碱基序列修饰获得 从病毒通过插入，替换或添加。 通过使用该方法构建含有病毒碱基序列的载体，能有效地产生蛋白质，而不会使包含载体的宿主细胞的生长恶化。

    SYSTEM FOR EXPRESSION OF GENES IN PLANTS
    30.
    发明申请
    SYSTEM FOR EXPRESSION OF GENES IN PLANTS 有权
    在植物中表达基因的系统

    公开(公告)号：US20120096585A1

    公开(公告)日：2012-04-19

    申请号：US13243796

    申请日：2011-09-23

    申请人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    发明人： Oleg Fedorkin ,  Shailaja Rabindran ,  Vidadi Yusibov

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8203 ,  C07K14/61 ,  C07K14/62 ,  C12N7/00 ,  C12N15/8257 ,  C12N2770/00043 ,  C12P21/02

    摘要： The present invention provides trans-complementation systems for expressing gene products in plants. In general, the invention provides systems including a carrier vector and a producer vector, both based on plant viruses. The producer vector is defective for at least one function needed for successful systemic infection of a plant, e.g., replication, cell-to-cell movement, or long distance movement. The carrier vector supplies the missing function in trans. Certain producer vectors lack a functional coat protein coding sequence, in which case the corresponding producer vector supplies coat protein in trans. The invention also provides novel plant viral vectors and methods of use, e.g., to produce polypeptides or active RNAs in plants.

    摘要翻译： 本发明提供用于在植物中表达基因产物的反式互补系统。 通常，本发明提供了包括载体载体和生产者载体的系统，其都是基于植物病毒的。 生产者载体对于植物的成功的全身感染所需的至少一个功能是有缺陷的，例如复制，细胞到细胞运动或长距离运动。 载波向量以反向方式提供丢失的功能。 某些生产者载体缺乏功能外壳蛋白编码序列，在这种情况下，相应的生产者载体以反式提供外壳蛋白。 本发明还提供新的植物病毒载体和使用方法，例如在植物中产生多肽或活性RNA。