公开(公告)号：US20170283822A1

公开(公告)日：2017-10-05

申请号：US15600193

申请日：2017-05-19

申请人： YISSUM RESEARCH DEVELOPMENT COMPANY OF THE HEBREW UNIVERSITY OF JERUSALEM LT

发明人： Oded Shoseyov ,  Lena MAGRISSO ,  Tzvi ZVIRIN

IPC分类号： C12N15/82 ,  C12N9/24 ,  C12N15/62 ,  C07K16/22 ,  C07K16/24

CPC分类号： C12N15/8258 ,  C07K16/22 ,  C07K16/241 ,  C07K2317/13 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2319/02 ,  C07K2319/03 ,  C07K2319/705 ,  C12N9/2402 ,  C12N15/62 ,  C12N15/625 ,  C12N15/8205 ,  C12N15/8218 ,  C12N15/8257 ,  C12P21/005 ,  C12Y302/01037 ,  C12Y302/01051

摘要： The A method of modifying a glycosylation pattern of a polypeptide-of-interest in a plant or plant cell is provided. The method comprising expressing in a plant or plant cell transformed to express at least one glycosidase in a subcellular compartment, a nucleic acid sequence encoding the polypeptide-of-interest, such that the at least one glycosidase and the polypeptide-of-interest are co-localized to the subcellular compartment of the plant or plant cell, thereby modifying the glycosylation pattern of the polypeptide-of-interest in the plant or plant cell.

公开(公告)号：US09765315B2

公开(公告)日：2017-09-19

申请号：US14421774

申请日：2013-08-15

申请人： Bangladesh Jute Research Institute

发明人： Maqsudul Alam ,  Mohammed S. Islam ,  Mohammed M. Hossen ,  Mohammed S. Haque ,  Mohammed M. Alam

IPC分类号： C07H21/02 ,  C12N9/42 ,  C12N9/24 ,  C12N9/26 ,  C12N9/34 ,  C12N9/38 ,  C12N9/88

CPC分类号： C12N9/2402 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2437 ,  C12N9/244 ,  C12N9/2445 ,  C12N9/2471 ,  C12N9/2482 ,  C12N9/2494 ,  C12N9/88 ,  C12Y302/01037 ,  C12Y302/01051 ,  C12Y302/01055 ,  C12Y302/01059 ,  C12Y302/01075 ,  C12Y302/01089 ,  C12Y302/0113 ,  C12Y302/01177

摘要： The present invention provides nucleotide sequences of Macrophomina phaseolina (“M. phaseolina”) that encodes proteins/enzymes with cellulolytic activity, including a cellulase activity, a endoglucanase, a cellobiohydrolase, a β-glucosidase, a a-glucosidase, a xylanase, a mannanse, a β-xylosidase, a a-xylosidase, a galactosidase, an arabinofuranosidase, a a-fucosidases, a β-galactanase, an unsaturated β-glucuronyl hydrolase and/or oligomerase activity. Vectors, expression constructs and host cells comprising and/or consisting of the nucleotide sequences of the enzyme genes are also provided. The invention further provides methods for producing the enzymes and methods for modifying the enzymes in order to improve their desirable characteristics. The enzymes of the invention can be used in a variety of, but not limited to, pharmaceutical, agricultural, food and feed processing, biofuel, energy efficiency and industrial contexts. These enzymes are also useful for complete hydrolysis of lignocellulosic biomass into simple sugar that can then be fermented to liquid fuels and chemical feedstocks.

公开(公告)号：US20160208302A1

公开(公告)日：2016-07-21

申请号：US14915421

申请日：2014-09-05

申请人： GLYCOM A/S

发明人： Gyula Dekany ,  Pauline Peltier-Pain ,  Dóra Molnár-Gábor ,  Markus Hederos

IPC分类号： C12P19/18 ,  C07H3/02 ,  C07H3/06 ,  C12P19/04

CPC分类号： C12P19/18 ,  C07H1/00 ,  C07H3/02 ,  C07H3/06 ,  C12P19/00 ,  C12P19/04 ,  C12Y302/01051

摘要： The present application discloses a method of making a mixture of 2′-FL and DFL in high yield by culturing, with lactose, a genetically modified cell having a recombinant gene that encodes a single fucosyl transferase. The resulting mixture of 2′-FL and DFL can be subjected to hydrolysis initiated by an acid or mediated by a fucosidase to produce fucose in high yields.

摘要翻译： 本申请公开了通过用乳糖培养具有编码单个岩藻糖基转移酶的重组基因的遗传修饰的细胞，以高产率制备2'-FL和DFL的混合物的方法。 所得到的2'-FL和DFL的混合物可以进行由酸引发的水解或由岩藻糖苷酶介导的产生高产率岩藻糖的水解。

公开(公告)号：US20150344585A1

公开(公告)日：2015-12-03

申请号：US14723020

申请日：2015-05-27

申请人： Academia Sinica

发明人： Chi-Huey WONG ,  Chung-Yi Wu ,  Ming-Hung Tsai

IPC分类号： C07K16/28 ,  C12P21/00

CPC分类号： C07K16/2887 ,  A61K2039/505 ,  A61K2039/54 ,  A61K2039/545 ,  C07K16/283 ,  C07K2317/24 ,  C07K2317/31 ,  C07K2317/41 ,  C07K2317/732 ,  C07K2317/734 ,  C07K2317/92 ,  C12P21/005 ,  C12Y302/01 ,  C12Y302/01051

摘要： The present disclosure relates to a novel class of anti-CD20 monoclonal antibodies comprising a homogeneous population of anti-CD20 IgG molecules having the same N-glycan on each of Fc. The antibodies of the invention can be produced from anti-CD20 monoclonal antibodies by Fc glycoengineering. Importantly, the antibodies of the invention have improved therapeutic values with increased ADCC activity and increased Fc receptor binding affinity compared to the corresponding monoclonal antibodies that have not been glycoengineered.

摘要翻译： 本公开涉及一类新的抗CD20单克隆抗体，其包含在Fc上具有相同N-聚糖的均匀的抗CD20IgG分子群。 本发明的抗体可以通过Fc糖工程从抗CD20单克隆抗体产生。 重要的是，本发明的抗体具有改善的治疗价值，与尚未糖基化的相应单克隆抗体相比，具有增加的ADCC活性和增加的Fc受体结合亲和力。

公开(公告)号：US08999705B2

公开(公告)日：2015-04-07

申请号：US13720866

申请日：2012-12-19

申请人： The Regents of the University of California

发明人： David A. Mills ,  Carlito B. Lebrilla ,  J. Bruce German ,  David Sela

IPC分类号： C12N9/24 ,  C12P19/00 ,  C07H21/04 ,  A23C9/20 ,  A23L1/29 ,  C12N9/18 ,  C12N9/88 ,  C12N9/90 ,  C12P19/04 ,  C12N9/38

CPC分类号： C12N9/24 ,  A23C9/206 ,  A23L33/40 ,  A23V2002/00 ,  C12N9/18 ,  C12N9/2402 ,  C12N9/2471 ,  C12N9/88 ,  C12N9/90 ,  C12P19/00 ,  C12P19/04 ,  C12Y301/01053 ,  C12Y302/01018 ,  C12Y302/01023 ,  C12Y302/01051 ,  C12Y302/01052 ,  C12Y403/03007 ,  C12Y501/03009 ,  A23V2250/28 ,  A23V2200/3202

摘要： This invention provides nucleic acids and proteins involved in oligosaccharide modification in the species Bifidobacteria. The invention provides methods for utilizing the proteins of the invention to generate human milk oligosaccharides or oligosaccharide mimics. The invention also provides compositions containing the human milk oligosaccharides or oligosaccharide mimics and methods for use.

摘要翻译： 本发明提供了在双歧杆菌物种中参与寡糖修饰的核酸和蛋白质。 本发明提供了利用本发明的蛋白质产生人乳低聚糖或寡糖模拟物的方法。 本发明还提供含有人乳低聚糖或寡糖模拟物的组合物和使用方法。

公开(公告)号：US20070122875A1

公开(公告)日：2007-05-31

申请号：US10518057

申请日：2003-06-20

申请人： Takeshi Sakai ,  Kumiko Ishizuka ,  Ikunoshin Kato

发明人： Takeshi Sakai ,  Kumiko Ishizuka ,  Ikunoshin Kato

IPC分类号： C12P21/06 ,  C12P19/04 ,  C12N9/24 ,  C12N1/21 ,  C07H21/04 ,  C08B37/00

CPC分类号： C12P19/04 ,  C12N9/16 ,  C12N9/2402 ,  C12N9/88 ,  C12P11/00 ,  C12Y302/01044 ,  C12Y302/01051

摘要： Sulfated fucan lyase capable of decomposing sulfated fucan derived from sea cucumber; a process for producing the enzyme; a low molecular compound obtained by causing the enzyme to act on sulfated fucan; a process for producing the same; and an activating factor for sulfated fucan lyase.

摘要翻译： 硫酸化岩藻糖裂解酶能够分解来自海参的硫酸化fucan; 一种生产酶的方法; 通过使酶作用于硫酸化的岩藻糖获得的低分子化合物; 其制造方法; 和硫酸化岩藻糖裂解酶的活化因子。

公开(公告)号：US07094563B2

公开(公告)日：2006-08-22

申请号：US10003136

申请日：2001-11-15

申请人： Sharon T. Wong-Madden ,  Ellen P. Guthrie ,  David Landry ,  Christopher H. Taron ,  Chudi Guan ,  Phillips W. Robbins

发明人： Sharon T. Wong-Madden ,  Ellen P. Guthrie ,  David Landry ,  Christopher H. Taron ,  Chudi Guan ,  Phillips W. Robbins

IPC分类号： C12P1/00 ,  C12P19/22 ,  C12N9/26 ,  C12N1/00 ,  C12N1/12

CPC分类号： C12Y302/01051 ,  C12N9/2402 ,  C12Y302/0105 ,  Y10S435/91

摘要： Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNacβ1-x, Galα1-3R, Galα1-6R, Galβ1-3R, Fucα-2R, Fucα1-3R, Fucα1-4R, Manα1-2R, Manα1-3R, Manα1-6R, Manβ1-4R, Xylβ1-2R, Glcβ1-4R, and Galβ1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

公开(公告)号：US06342365B1

公开(公告)日：2002-01-29

申请号：US09257153

申请日：1999-02-24

申请人： Sharon T. Wong-Madden ,  Ellen P. Guthrie ,  David Landry ,  Christopher H. Taron ,  Chudi Guan ,  Phillips W. Robbins

发明人： Sharon T. Wong-Madden ,  Ellen P. Guthrie ,  David Landry ,  Christopher H. Taron ,  Chudi Guan ,  Phillips W. Robbins

IPC分类号： C12Q134

CPC分类号： C12Y302/01051 ,  C12N9/2402 ,  C12Y302/0105 ,  Y10S435/91

摘要： Substantially pure glycosidases capable for cleaving selected glycosidic bonds have been described including glycosidases isolated from Xanthomonas and recombinant glycosidases. Substrate specificity of isolated enzymes have been identified for GlcNac&bgr;1-X, Gal&agr;1-3R, Gal&agr;1-6R, Gal&bgr;1-3R, Fuc&agr;1-2R, Fuc&agr;1-3R, Fuc&agr;1-4R, Man&agr;1-2R, Man&agr;1-3R, Man&agr;1-6R, Man&bgr;1-4R, Xyl&bgr;1-2R, Glc&bgr;1-4R, and Gal&bgr;1-4R providing improved capability for selectively cleaving a glycosidic linkage in a carbohydrate substrate and for forming modified carbohydrates.

摘要翻译： 已经描述了能够切割选择的糖苷键的基本上纯的糖苷酶，其包括从黄单胞菌属和重组糖苷酶分离的糖苷酶。 已经鉴定了GlcNacbeta1-X，Galalpha1-3R，Galalpha1-6R，Galbeta1-3R，Fucalpha1-2R，Fucalpha1-3R，Fucalpha1-4R，Manalpha1-2R，Manalpha1-3R，Manalpha1-6R，Manbeta1的分离的酶的底物特异性 -4R，Xylbeta1-2R，Glcbeta1-4R和Galbeta1-4R，其提供了选择性地切割碳水化合物底物中的糖苷键并形成修饰的碳水化合物的改善的能力。

公开(公告)号：US20190201503A1

公开(公告)日：2019-07-04

申请号：US16353168

申请日：2019-03-14

申请人： Ellis KLINE

发明人： Ellis KLINE

IPC分类号： A61K38/47 ,  A61K9/00 ,  A61K45/06 ,  A61K31/5377 ,  A61K31/517 ,  A61K31/506 ,  A61K31/404

CPC分类号： A61K38/47 ,  A61K9/006 ,  A61K31/404 ,  A61K31/506 ,  A61K31/517 ,  A61K31/5377 ,  A61K45/06 ,  C12Y302/01018 ,  C12Y302/01023 ,  C12Y302/01049 ,  C12Y302/0105 ,  C12Y302/01051 ,  A61K2300/00

摘要： The present invention relates to treatment and prevention of cancer with glycosidase(s). In various aspects the invention relates to the treatment and management of cancer to prevent metastasis or recurrence, or to improve outcome and rate of successful treatment with conventional therapeutic regimens.

公开(公告)号：US20180265910A1

公开(公告)日：2018-09-20

申请号：US15462287

申请日：2017-03-17

申请人： New England Biolabs, Inc.

发明人： Christopher H. Taron ,  Saulius Vainauskas ,  Xiaofeng Shi

IPC分类号： C12Q1/40 ,  C12Q1/34

CPC分类号： C12Q1/40 ,  C12Q1/34 ,  C12Y302/01018 ,  C12Y302/01022 ,  C12Y302/01023 ,  C12Y302/01051 ,  C12Y302/01096 ,  C12Y305/01052 ,  G01N2333/924 ,  G01N2400/02 ,  G01N2440/38

摘要： Provided herein is an α-fucosidase that can cleave a conjugate comprising an N-glycan and a label where the label is added by amine reactive chemistry. The α-fucosidase also has an accelerated reaction time using Schiff base labeled N-glycans compared with BKF. A reaction mix, enzyme mix and kit comprising the α-fucosidase are provided, as well as a method for analyzing glycoproteins. The α-fucosidase finds particular use in analyzing the N-glycans of therapeutic glycoproteins.