公开(公告)号：US20160076096A1

公开(公告)日：2016-03-17

申请号：US14856623

申请日：2015-09-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Min-Yi Shen ,  Douglas Greiner

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01J2219/005 ,  C12Q1/6874 ,  C12Q2533/107 ,  C12Q2535/122 ,  C12Q2545/101 ,  G01N2201/1242 ,  G16B30/00 ,  C12Q2563/149

Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.

Abstract translation: 公开了一种用于进行核酸样品测序的质量控制的系统。 该系统包含一组固体支持物，每个固体支持物附着有多个核酸序列，其中该组包含多组固体支持物，并且每组含有与其相连的相同核酸序列的固体支持物。 各组的核酸序列彼此不同。 核酸序列是合成衍生的，并且设计核酸序列使得核酸序列在测序运行期间产生可检测信号的预定模式。 还公开了制备用于进行核酸样品测序的质量控制的方法，在核酸测序实验期间验证核酸测序仪的方法，以及在核酸测序实验期间处理核酸测序数据的方法。

公开(公告)号：US20150346085A1

公开(公告)日：2015-12-03

申请号：US14648855

申请日：2013-12-04

Applicant: SP3H

Inventor： Sylvain OBERTI ,  Johan FOURNEL

IPC: G01N21/27 ,  G01N21/3504

CPC classification number: G01N21/3577 ,  G01N21/274 ,  G01N21/3504 ,  G01N2021/3181 ,  G01N2201/062 ,  G01N2201/0624 ,  G01N2201/121 ,  G01N2201/1211 ,  G01N2201/124 ,  G01N2201/1242 ,  G01N2201/1247 ,  G01N2201/127 ,  G01N2201/12723

Abstract: A method for controlling a spectrometer for analyzing a product includes steps of: acquiring a measurement representative of the operation of a light source, determining, depending on the measurement, a value of supply current of the light source, and/or a value of integration time of light-sensitive cells of a sensor, disposed on a route of a light beam emitted by the light source and having interacted with a product to be analyzed, and if the integration time and/or supply current value is between threshold values, supplying the light source with a supply current corresponding to the determined supply current value, adjusting the integration time of a light-sensitive cell to the determined integration time value, and acquiring light intensity measurements supplied by the sensor, enabling a spectrum to be formed.

Abstract translation: 一种用于控制用于分析产品的光谱仪的方法包括以下步骤：获取代表光源的操作的测量，根据测量确定光源的电源电流的值和/或积分值 传感器的感光单元的时间设置在由光源发射的光束的路径上并且与待分析的产品相互作用，并且如果积分时间和/或电源电流值在阈值之间，则提供 所述光源具有与所确定的电源电流值相对应的电源电流，将感光单元的积分时间调整到所确定的积分时间值，以及获取由所述传感器提供的光强度测量值，从而能够形成光谱。

公开(公告)号：US20110207624A1

公开(公告)日：2011-08-25

申请号：US13030818

申请日：2011-02-18

Applicant: Min-Yi Shen ,  Douglas Greiner

Inventor： Min-Yi Shen ,  Douglas Greiner

IPC: C40B30/04 ,  C40B40/08 ,  C40B50/14

CPC classification number: C12Q1/6874 ,  B01J2219/005 ,  C12Q2533/107 ,  C12Q2535/122 ,  G01N2201/1242 ,  G06F19/22 ,  C12Q2563/149 ,  C12Q2545/101

Abstract: A system for performing quality control for nucleic acid sample sequencing is disclosed. The system comprises a set of solid supports, each solid support having attached thereto a plurality of nucleic acid sequences, wherein the set comprises plural groups of solid supports and each group contains solid supports having the same nucleic acid sequences attached thereto. The nucleic acid sequences of each group differ from each other. The nucleic acid sequences are synthetically derived, and the nucleic acids sequences are designed such that the nucleic acid sequences produce a predefined pattern of detectable signals during a sequencing run. A method of preparing a quality control for performing nucleic acid sample sequencing, a method of validating a nucleic acid sequencing instrument during a nucleic acid sequencing experiment, and a method of processing nucleic acid sequencing data during a nucleic acid sequencing experiment are also disclosed.

Abstract translation: 公开了一种用于进行核酸样品测序的质量控制的系统。 该系统包含一组固体支持物，每个固体支持物附着有多个核酸序列，其中该组包含多组固体支持物，并且每组含有与其相连的相同核酸序列的固体支持物。 各组的核酸序列彼此不同。 核酸序列是合成衍生的，并且设计核酸序列使得核酸序列在测序运行期间产生可检测信号的预定模式。 还公开了制备用于进行核酸样品测序的质量控制的方法，在核酸测序实验期间验证核酸测序仪的方法，以及在核酸测序实验期间处理核酸测序数据的方法。

公开(公告)号：US07521703B2

公开(公告)日：2009-04-21

申请号：US11653949

申请日：2007-01-17

Applicant: Kenichi Nishigaki ,  Masato Ishizawa ,  Shigenori Watari

Inventor： Kenichi Nishigaki ,  Masato Ishizawa ,  Shigenori Watari

IPC: G01J1/42

CPC classification number: G01N21/253 ,  G01N21/272 ,  G01N35/00603 ,  G01N2201/0415 ,  G01N2201/1242 ,  G01N2201/1245

Abstract: In an automatic analyzer which includes a reaction container which contains reaction solution therein, a light source which emit light to be transmitted through the reaction solution, a spectral detector which measures the light transmitted through the reaction solution, a memory which stores light measurement data measured by the spectral detector and a CPU which calculates the light measurement data stored in the memory to obtain a light intensity, wherein the spectral detector measures the light over an entirety of an area from one end to the other end of the reaction container at a portion where the reaction solution reserves, the memory stores the light measurement data measured by the spectral detector, and light measurement data in an area where the reaction solution exists is obtained from the memory to calculate a light intensity.

Abstract translation: 在包含反应容器的自动分析装置中，发射透过反应溶液的光的光源，测定透过反应液的光的光谱检测器，存储测定的光测量数据的存储器 通过光谱检测器和CPU，其计算存储在存储器中的光测量数据以获得光强度，其中光谱检测器在一部分处测量反射容器的一端至另一端的整个区域的光 在反应溶液保留的情况下，存储器存储由光谱检测器测量的光测量数据，并且从存储器获得存在反应溶液的区域中的光测量数据，以计算光强度。

公开(公告)号：US20070181787A1

公开(公告)日：2007-08-09

申请号：US11653949

申请日：2007-01-17

Applicant: Kenichi Nishigaki ,  Masato Ishizawa ,  Shigenori Watari

Inventor： Kenichi Nishigaki ,  Masato Ishizawa ,  Shigenori Watari

IPC: G01J1/42 ,  G01J5/08

CPC classification number: G01N21/253 ,  G01N21/272 ,  G01N35/00603 ,  G01N2201/0415 ,  G01N2201/1242 ,  G01N2201/1245

Abstract: In an automatic analyzer which includes a reaction container which contains reaction solution therein, a light source which emit light to be transmitted through the reaction solution, a spectral detector which measures the light transmitted through the reaction solution, a memory which stores light measurement data measured by the spectral detector and a CPU which calculates the light measurement data stored in the memory to obtain a light intensity, wherein the spectral detector measures the light over an entirety of an area from one end to the other end of the reaction container at a portion where the reaction solution reserves, the memory stores the light measurement data measured by the spectral detector, and light measurement data in an area where the reaction solution exists is obtained from the memory to calculate a light intensity.

Abstract translation: 在包含反应容器的自动分析装置中，发射透过反应溶液的光的光源，测定透过反应液的光的光谱检测器，存储测定的光测量数据的存储器 通过光谱检测器和CPU，其计算存储在存储器中的光测量数据以获得光强度，其中光谱检测器在一部分处测量反射容器的一端至另一端的整个区域的光 在反应溶液保留的情况下，存储器存储由光谱检测器测量的光测量数据，并且从存储器获得存在反应溶液的区域中的光测量数据，以计算光强度。

公开(公告)号：US07180594B2

公开(公告)日：2007-02-20

申请号：US10856885

申请日：2004-05-27

Applicant: Paul C. Williams

Inventor： Paul C. Williams

IPC: G01N21/00 ,  G01J3/42

CPC classification number: G01N21/534 ,  G01N21/274 ,  G01N21/4785 ,  G01N21/8507 ,  G01N2021/8535 ,  G01N2201/0218 ,  G01N2201/024 ,  G01N2201/1242 ,  G01N2201/12746

Abstract: An in-situ technique is provided for automatically verifying proper operation of a photometric device, such as a cell density probe (CDP). The CDP has a first detector and a second detector. The first detector senses light that is transmitted from a light source of the CDP. The second detector senses light that has passed through an optical gap at a tip of the CDP, wherein the sensed light has been reduced in intensity due to light absorption. Electrical current provided to the light source is reduced, and the resultant values of a light characteristic (such as intensity) are sensed. These values from the detectors are compared against one or more predicted values. If the CDP is operating properly, the values from the detectors will be consistent with the predicted values. If there is a malfunction in the CDP, then the values from the detectors will be inconsistent with the predicted values.

Abstract translation: 提供了用于自动验证诸如细胞密度探针（CDP）的光度测量装置的正确操作的原位技术。 CDP具有第一检测器和第二检测器。 第一检测器感测从CDP的光源传输的光。 第二检测器感测已经通过CDP顶端的光学间隙的光，其中感测的光由于光吸收而被强度降低。 降低了提供给光源的电流，并且感测出光特性（如强度）的合成值。 来自检测器的这些值与一个或多个预测值进行比较。 如果CDP正常工作，检测器的值将与预测值一致。 如果CDP出现故障，则检测器的值将与预测值不一致。

公开(公告)号：US20050264816A1

公开(公告)日：2005-12-01

申请号：US10856885

申请日：2004-05-27

Applicant: Paul Williams

Inventor： Paul Williams

IPC: C12M1/34 ,  G01J1/32 ,  G01N21/27 ,  G01N21/53 ,  G01N21/85 ,  G01B9/02

CPC classification number: G01N21/534 ,  G01N21/274 ,  G01N21/4785 ,  G01N21/8507 ,  G01N2021/8535 ,  G01N2201/0218 ,  G01N2201/024 ,  G01N2201/1242 ,  G01N2201/12746

Abstract: An in-situ technique is provided for automatically verifying proper operation of a photometric device, such as a cell density probe (CDP). The CDP has a first detector and a second detector. The first detector senses light that is transmitted from a light source of the CDP. The second detector senses light that has passed through an optical gap at a tip of the CDP, wherein the sensed light has been reduced in intensity due to light absorption. Electrical current provided to the light source is reduced, and the resultant values of a light characteristic (such as intensity) at the first and second detectors are sensed. These values from the detectors are compared against one or more predicted values. If the CDP is operating properly, the values from the detectors will be consistent with the predicted values. If there is a malfunction in the CDP, then the values from the detectors will be inconsistent with the predicted values.

Abstract translation: 提供了用于自动验证诸如细胞密度探针（CDP）的光度测量装置的正确操作的原位技术。 CDP具有第一检测器和第二检测器。 第一检测器感测从CDP的光源传输的光。 第二检测器感测已经通过CDP顶端的光学间隙的光，其中感测的光由于光吸收而被强度降低。 提供给光源的电流减小，并且感测在第一和第二检测器处的​​光特性（例如强度）的合成值。 来自检测器的这些值与一个或多个预测值进行比较。 如果CDP正常工作，检测器的值将与预测值一致。 如果CDP出现故障，则检测器的值将与预测值不一致。