公开(公告)号：US11950553B2

公开(公告)日：2024-04-09

申请号：US16449329

申请日：2019-06-21

Applicant: Keygene N.V.

Inventor： Jeroen Stuurman

IPC: A01H1/02 ,  A01H3/00 ,  A01H6/82 ,  A01H1/04 ,  A01H5/10

CPC classification number: A01H1/021 ,  A01H3/00 ,  A01H6/82 ,  A01H1/04 ,  A01H5/10

Abstract: The present invention provides for a method for producing an inbred plant comprising a first and second trait of interest in the L1-shoot meristem layer for use in producing a periclinal chimera plant, the inbred plant thus obtained, the use of said inbred plant for producing said periclinal chimera plant, a method for producing a periclinal chimera plant using said inbred plant, a periclinal chimera plant thus obtained, the use of said periclinal chimera plant in producing plant product and the plant product thus obtained.

公开(公告)号：US11844323B2

公开(公告)日：2023-12-19

申请号：US17701154

申请日：2022-03-22

Applicant: PureCircle USA Inc. ,  KEYGENE N.V. ,  THE COCA-COLA COMPANY

Inventor： Avetik Markosyan ,  Seong Siang Ong ,  Runchun Jing ,  Tengfang Huang ,  Stephen Ezra Schauer ,  Fayaz Khazi ,  Indra Prakash ,  Alec Hayes

IPC: A01H6/14 ,  C12N15/82

CPC classification number: A01H6/1488 ,  C12N15/8245 ,  C12N15/8279

Abstract: Stevia varieties with a high content of RebM, are disclosed Further provided are methods for producing Stevia plants having a high RebM content by negatively regulating certain genes selecting the resulting plants, and breeding with such plants to confer such desirable Reb M phenotypes to plant progeny.

公开(公告)号：US20220333100A1

公开(公告)日：2022-10-20

申请号：US17843612

申请日：2022-06-17

Applicant: Keygene N.V.

Inventor： René Cornelis Josephus HOGERS ,  Stefan John WHITE

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6855

Abstract: The current invention pertains to adapters comprising a protelomerase recognition sequence, preferably a TeIN protelomerase recognition sequence. The adapters of the invention can be used for the preparation of a nucleic acid molecule library. The invention also relates to a method for producing a nucleic acid molecule library using one or more adapters comprising a protelomerase recognition sequence. The adapters may be contacted with a protelomerase to cleave and close the ends of the adapters. Said closed adapters are e.g. protected against exonuclease treatment. The method of the invention further concerns an amplification method and a sequencing method using adapters having a protelomerase recognition sequence.

公开(公告)号：US20220315940A1

公开(公告)日：2022-10-06

申请号：US17843627

申请日：2022-06-17

Applicant: Keygene N.V. ,  Stichting Wageningen Research

Inventor： Hendrik Jan BOSCH ,  Michiel Theodoor Jan DE BOTH ,  Katarina CANKAR ,  Paul BUNDOCK ,  Robert Edouard SEVENIER ,  Martinus Julius BEEKWILDER

IPC: C12N15/82 ,  C12N15/11 ,  C12N9/22 ,  C12N9/88

Abstract: The invention is in the field of agriculture, in particular in the field of crop improvement for processing, more particularly in the field of sesquiterpene lactone (STL), squalene and phenolic compound biosynthesis by plants. A method for producing a plant having reduced STL levels, increased squalene levels and increased phenolic compound levels is disclosed, as well as a plant produced by such method.

公开(公告)号：US20220025445A1

公开(公告)日：2022-01-27

申请号：US17402037

申请日：2021-08-13

Applicant: Keygene N.V.

Inventor： Stefan John WHITE ,  René Cornelis Josephus HOGERS

IPC: C12Q1/6827 ,  C12Q1/6869 ,  G16B20/20 ,  G16B25/20

Abstract: The current invention pertains to a reliable method for determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell, wherein the method uses a UMI to correct for any amplification biases. The invention further pertains to the use of a UMI for accurately determining the relative frequency of a sequence variant of interest in a nucleic acid sample derived from at least one polyploid cell.

公开(公告)号：US11060100B2

公开(公告)日：2021-07-13

申请号：US16310349

申请日：2017-06-16

Applicant: Keygene N.V.

Inventor： Matthew Vitabile Dileo ,  Jesse David Munkvold ,  Martin De Vos ,  Anna Maria Tomczak ,  Sandra Goritschnig

IPC: C12N15/82 ,  C07K14/41 ,  C07K14/415

Abstract: The invention is in the field of agriculture, in particular in the field of crop protection, more particularly in the field of providing nematode resistance to plants. A method for producing a plant having improved nematode resistance, particularly to root-knot nematodes and/or cyst nematodes, is disclosed, as well as a plant produced by such method.

公开(公告)号：US20210079415A1

公开(公告)日：2021-03-18

申请号：US17062221

申请日：2020-10-02

Applicant: Keygene N.V.

Inventor： Michiel Theodoor Jan DE BOTH ,  Rik Hubertus Martinus OP DEN CAMP ,  Bjorn Alexander KLOOSTERMAN ,  Bernarda Gerharda Johanna FIERENS-ONSTENK

IPC: C12N15/82

Abstract: Genetic modification of plants is hampered by the limited capacity of plant cells to regenerate. The current invention solves this problem by introducing or increasing the expression of a histidine kinase in a plant cell. Preferred histidine kinases are at least one of CHK2, CHK3 and CHK4. The invention therefore concerns a method for improving a cytokinin-induced regeneration capacity of a plant cell, wherein the method comprises a step of increasing or introducing the expression of a histidine kinase in the plant cell. The invention further pertains to a method for regenerating a plant, wherein the method comprises a step of introducing or increasing the expression of a histidine kinase and to a plant obtainable from such method. Moreover, the method concerns the use of at least one of CHK2, CHK3 and CHK4 for improving a cytokinin-induced regeneration capacity of a plant.

公开(公告)号：US20210054393A1

公开(公告)日：2021-02-25

申请号：US17081832

申请日：2020-10-27

Applicant: Keygene N.V.

Inventor： Bernardus SCHERES ,  Renze HEIDSTRA ,  Menno Christian Cyrano PIJNENBURG ,  Michiel Theodoor Jan DE BOTH

IPC: C12N15/82 ,  C07K14/415

Abstract: The invention pertains to a method for regenerating a plant cell, preferably regenerating a shoot from a plant cell by altering the expression levels of at least WOX5 and a PLT protein, preferably WOX5 and PLT1. In addition the expression levels of further proteins can altered, such as WIND1, SHR, SCR, RBR, PLT4 and PLT5 to regenerate a shoot from a plant cell. Preferably, the expression levels are transiently altered. The invention further pertains to a nucleic acid construct suitable for transient protein expression and the use of the protein combinations for regenerating a shoot from a plant cell.

公开(公告)号：US20200291471A1

公开(公告)日：2020-09-17

申请号：US16719863

申请日：2019-12-18

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  Adrianus Johannes VAN TUNEN

IPC: C12Q1/6874 ,  G16B30/00 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q1/6827 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6851

Abstract: Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation.

公开(公告)号：US20200071757A1

公开(公告)日：2020-03-05

申请号：US16539119

申请日：2019-08-13

Applicant: Keygene N.V.

Inventor： Michael Josephus Theresia VAN EIJK ,  René Cornelis Josephus HOGERS

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6827

Abstract: The invention relates to a method for the detection of a target nucleotide sequence in a sample based on an oligonucleotide ligation assay wherein probes are used that contain (a combination of) sequence-based identifiers that can identify the sample and the target sequence (i.e. locus and/or allele combination) wherein after the ligation step, the ligated probes, or after amplification, the amplified ligated probes, are restricted using restriction enzymes to cut of part of the probes and continue with those parts (identifiers and target sequence) that contain the relevant information in the sequencing step.