-
公开(公告)号：US11697796B2
公开(公告)日：2023-07-11
申请号：US16480025
申请日：2018-01-23
Applicant: STEMCELL Technologies Canada Inc.
Inventor： Adam Hirst , Arwen Hunter , Melanie Kardel , Wing Chang
IPC: C12N5/0735 , C12N5/074
CPC classification number: C12N5/0606 , C12N5/0696 , C12N2500/36 , C12N2501/115 , C12N2501/15 , C12N2501/727 , C12N2501/999 , C12N2533/90
Abstract: The present disclosure relates to improved supplements, culture media and methods for enhancing the survival or proliferation of mammalian stem cells. In particular, adding a lipid supplement, such as a lipid-enriched carrier (e.g. a lipid-enriched albumin), to the culture medium may enhance the survival and/or proliferation of the stem cells by at least 5% to 65% as compared to a culture medium that does not contain the lipid supplement.
-
公开(公告)号：US20220315877A1
公开(公告)日：2022-10-06
申请号：US17620511
申请日：2019-12-18
Applicant: StemCell Technologies Canada Inc.
Inventor： Emanuel NAZARETH , Eric JERVIS , Martin O'KEANE , Tia SOJONKY , Mark ROMANISH
Abstract: Apparatus, systems and methods for the adaptive passage of a culture of cells and apparatus and methods for dissociating cell colonies are described. The systems may include an imaging module, a pipette module, a handling module, and/or a stage module. Coordinated operation of the modules, optionally in an automated manner, is effected by at least one processor based on one or more characteristics of the culture of cells calculated from one or more images captured at more than one time point. A first apparatus for adaptive passage of a culture cells includes an imaging module and at least one processor, which apparatus may be included in the systems or used in the methods. A second apparatus for dissociating cell colonies, may also be included in the systems or used in the methods, includes impact bumper(s) collidable with impact bracket(s) to transmit a dissociative force to a culture of cells.
-
公开(公告)号：US20210317399A1
公开(公告)日：2021-10-14
申请号：US17253838
申请日：2019-06-19
Applicant: STEMCELL TECHNOLOGIES CANADA INC.
Inventor： Emanuel Nazareth , Eric Jervis , Martin O'Keane , Carrick Faris
Abstract: Systems and methods for the automated culture of cells and apparatus and methods for dissociating one or more colonies in a culture of cells are described. The automated systems include at least an imaging module, a pipette module, a handling module, and optionally a stage module. Coordinated operation of the foregoing modules is effected by at least one processor based on one or more characteristics of the culture of cells calculated from one or more images captured at a first time point and at one or more subsequent time points. The apparatus includes a mounting surface, having one or more impact bumpers, connected to a rotatable drive, a platform rotatable dependently or independently of the drive, and one or more impact brackets connected to the platform and collidable with the one or more impact bumpers to transmit a dissociative force to a culture of cells in a cell culture vessel upon the platform.
-
公开(公告)号：US20190178879A1
公开(公告)日：2019-06-13
申请号：US16227436
申请日：2018-12-20
Applicant: StemCell Technologies Canada Inc.
Inventor： Samuel Jon Clarke , Adil Kassam
IPC: G01N33/533 , G01N33/569
Abstract: This disclosure provides compositions and methods for a low-avidity, high-affinity and high-specificity biomolecular interaction that is rapidly reversible under physiological conditions. The methods comprise linking biological targets (such as molecules, proteins, DNA, cells, extracellular vesicles, etc.) with polymers and anti-polymer ligands and a way to reverse their binding using physiologically compatible polymeric compounds. The methods also comprise a way to combine different polymer/anti-polymer systems for orthogonal labeling. The compositions comprise labels including particles (fluorescent, magnetic, dense, etc.) conjugated to polymers or labels conjugated to anti-polymer antibodies. The compositions also comprise biomolecules (proteins, antibodies, DNA, etc.) conjugated to the polymers. These methods and compositions represent a major improvement to the state-of-the-art. They are particularly useful for separation and isolation of biological targets using particles, but have important application to other fields including fluorescent imaging.
-
公开(公告)号：US12258552B2
公开(公告)日：2025-03-25
申请号：US17620511
申请日：2019-12-18
Applicant: StemCell Technologies Canada Inc.
Inventor： Emanuel Nazareth , Eric Jervis , Martin O'Keane , Tia Sojonky , Mark Romanish
Abstract: Apparatus, systems and methods for the adaptive passage of a culture of cells and apparatus and methods for dissociating cell colonies are described. The systems may include an imaging module, a pipette module, a handling module, and/or a stage module. Coordinated operation of the modules, optionally in an automated manner, is effected by at least one processor based on one or more characteristics of the culture of cells calculated from one or more images captured at more than one time point. A first apparatus for adaptive passage of a culture cells includes an imaging module and at least one processor, which apparatus may be included in the systems or used in the methods. A second apparatus for dissociating cell colonies, may also be included in the systems or used in the methods, includes impact bumper(s) collidable with impact bracket(s) to transmit a dissociative force to a culture of cells.
-
Patent Agency Ranking
公开(公告)号：US12150982B2
公开(公告)日：2024-11-26
申请号：US17563764
申请日：2021-12-28
Applicant: STEMCELL TECHNOLOGIES CANADA INC.
Inventor： Howard Bernstein , Scott Loughhead , Matthew Booty , Kelan Hlavaty , Tarek Abdeljawad , Maisam Dadgar , Jason Murray , David Chirgwin , Harry An , Armon R. Sharei
Abstract: The present application provides formulations of modified peripheral blood mononuclear cells (PBMCs), wherein the formulation comprises: PBMCs comprising at least one antigen (e.g., a human papillomavirus (HPV) antigen) and a cryopreservation medium. In some embodiments, the PBMCs are conditioned by incubating the PBMC in the presence of an adjuvant.
-
公开(公告)号：US20240342727A1
公开(公告)日：2024-10-17
申请号：US18739988
申请日：2024-06-11
Applicant: STEMCELL TECHNOLOGIES CANADA INC.
Inventor： Martin O'KEANE , Mark SMYTH , Kevin CHEN , Miguel EAGLETON
Abstract: Aspects of this disclosure relate to a vessel support apparatus and to uses of the vessel support, such as in the laboratory setting. Apparatus of this disclosure may secure a vessel thereto, such as when the apparatus is in operation or use. Vessel support apparatus will comprise a vessel supporting means and one or more means for securing the vessel to the supporting means.
-
公开(公告)号：US20230257707A1
公开(公告)日：2023-08-17
申请号：US18017512
申请日：2021-07-27
Applicant: STEMCELL TECHNOLOGIES CANADA INC.
Inventor： Nooshin TABATABAEI-ZAVAREH , Tim LE FEVRE
IPC: C12N5/0783 , C12N5/0781
CPC classification number: C12N5/0636 , C12N5/0646 , C12N5/0635 , C12N2501/26 , C12N2501/125 , C12N2501/145 , C12N2501/155 , C12N2501/115 , C12N2501/165 , C12N2500/90 , C12N2502/13 , C12N2506/02
Abstract: Disclosed are media, methods, systems and kits for differentiating hematopoietic progenitor cells from a population of early mesoderm cells under conditions that exclude any combination of certain exogenously added agonists of growth factor signaling. The population of early mesoderm cells may be derived from pluripotent stem cells, and the hematopoietic progenitor cells differentiated under the disclosed conditions are multipotent. The culture conditions disclosure herein may form serum-free workflows and/or workflows free of stroma and/or feeder cells.
-
公开(公告)号：US11725042B2
公开(公告)日：2023-08-15
申请号：US16912882
申请日：2020-06-26
Applicant: STEMCELL Technologies Canada Inc.
Inventor： Adil Kassam , Eric Jervis
IPC: C07K14/705 , C07K16/28 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/541 , G01N33/543 , G01N33/566 , G01N33/577 , A61K47/69 , C12M1/00 , C12M1/12 , C12M1/34 , G01N33/537
CPC classification number: C07K14/70596 , A61K47/6913 , C07K16/2896 , C12M21/00 , C12M25/14 , C12M41/36 , G01N33/48 , G01N33/50 , G01N33/53 , G01N33/537 , G01N33/541 , G01N33/54346 , G01N33/566 , G01N33/577 , C07K2319/03 , G01N2333/705 , G01N2333/70596
Abstract: A method of recovering a population of extracellular vesicles from a biological sample comprising extracellular vesicles and contaminants is described. In one embodiment, the method comprises: a) removing contaminants from the sample, wherein the contaminants are relatively larger or more dense than the extracellular vesicles; b) contacting the sample of step a) with a plurality of binding compositions, each binding composition having first and/or second moieties capable of binding a recognition motif of the target entities under conditions to allow complexing of the extracellular vesicles with the plurality of binding compositions to form a complexed population of extracellular vesicles, the complexed population of extracellular vesicles having an increased volume and/or higher density in comparison to the extracellular vesicles in individual form; and c) recovering the complexed population of extracellular vesicles.
-
公开(公告)号：US20230250194A1
公开(公告)日：2023-08-10
申请号：US18012557
申请日：2021-06-29
Applicant: StemCell Technologies Canada Inc.
Inventor： Eric OUELLET , Andy KOKAJI
CPC classification number: C07K16/468 , A61P37/02 , C07K2317/31
Abstract: Provided are compositions comprising a target cell binding moiety conjugated with a nucleic acid polymer, the nucleic acid polymer characterized by a predicted melting temperature range (of secondary structures thereof) and/or a predicted free energy range (of self-hybridization). The compositions may be included in kits or systems, together with one or more complementary oligonucleotides, competitor oligonucleotides, anti-competitor oligonucleotides or the like. The compositions, kits, and systems may be used in methods of stimulating cells, quenching the stimulation of cells, modulating the stimulation of cells, or tagging (stimulated or unstimulated) cells to be analyzed.
-
-
-
-
-
-
-
-
-
Search Results
41
records
(took 0.019 seconds)
