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    Sesquiterpene synthases from grand fir (abies grandis), and methods of use
    31.
    发明申请
    Sesquiterpene synthases from grand fir (abies grandis), and methods of use 有权

    公开(公告)号：US20020038001A1

    公开(公告)日：2002-03-28

    申请号：US09865171

    申请日：2001-05-24

    Applicant: Washington State University Research Foundation

    Inventor： Rodney Bruce Croteau ,  Jorg Bohlmann ,  John E. Crock ,  Christopher L. Steele

    IPC: C12P021/00

    CPC classification number: C12N15/8243 ,  C12N9/88 ,  C12N15/8279

    Abstract: cDNAs encoding E-null-bisabolelene synthase, null-selinene synthase and null-humulene synthase from Grand Fir (Abies grandis) have been isolated and sequenced, and the corresponding amino acid sequences have been determined. Accordingly, isolated DNA sequences (SEQ ID No:12; SEQ ID No:19 and SEQ ID No:23) are provided which code for the expression of E-null-bisabolene synthase (SEQ ID No:13), null-selinene synthase (SEQ ID No:20) and null-humulene synthase (SEQ ID No:24), respectively, from Grand Fir (Abies grandis). In other aspects, replicable recombinant cloning vehicles are provided which code for E-null-bisabolene synthase, null-selinene synthase and null-humulene synthase, or for a base sequence sufficiently complementary to at least a portion of E-null-bisabolene synthase, null-selinene synthase or null-humulene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding E-null-bisabolene synthase, null-selinene synthase or null-humulene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant sesquiterpene synthases that may be used to facilitate their production, isolation and purification in significant amounts. Recombinant E-null-bisabolene synthase, null-selinene synthase and null-humulene synthase may be used to obtain expression or enhanced expression of E-null-bisabolene synthase, null-selinene synthase and null-humulene synthase in plants in order to enhance the production of sesquiterpenoids, or may be otherwise employed for the regulation or expression of E-null-bisabolene synthase, null-selinene synthase and null-humulene synthase, or the production of their products.

    Secure key storage and distribution
    33.
    发明授权
    Secure key storage and distribution 有权
    安全密钥存储和分发

    公开(公告)号：US09049011B1

    公开(公告)日：2015-06-02

    申请号：US13967029

    申请日：2013-08-14

    Applicant: Washington State University Research Foundation

    Inventor： Punit Agrawal

    IPC: H04L29/00 ,  H04L9/08

    CPC classification number: H04L9/085 ,  H04L9/0825 ,  H04L9/0894

    Abstract: This disclosure describes a distributed, fault-tolerant security system that enables the secure storage and distribution of private keys. In one implementation, the security system includes a plurality of computing resources that independently store private keys provided by publishers and encrypted using a single security system public key. To protect against malicious activity, the security system private key necessary to decrypt the publication private keys is not stored at any of the computing resources. Rather portions, or shares of the security system private key are stored at each of the computing resources within the security system and multiple security systems must communicate and share partial decryptions in order to decrypt the stored private key.

    Abstract translation: 本公开描述了分布式，容错的安全系统，其实现私钥的安全存储和分发。 在一个实现中，安全系统包括多个计算资源，其独立地存储由发布者提供并且使用单个安全系统公钥加密的私钥。 为了防止恶意活动，解密发布私钥所需的安全系统私钥不会存储在任何计算资源上。 相反，安全系统私钥的部分或共享存储在安全系统内的每个计算资源上，并且多个安全系统必须通信和共享部分解密以便解密存储的私钥。

    Transacylases of the paclitaxel biosynthetic pathway
    35.
    发明申请
    Transacylases of the paclitaxel biosynthetic pathway 审中-公开
    紫杉醇生物合成途径的转酰胺酶

    公开(公告)号：US20040005562A9

    公开(公告)日：2004-01-08

    申请号：US10166984

    申请日：2002-06-10

    Applicant: Washington State University Research Foundation

    Inventor： Rodney B. Croteau ,  Kevin D. Walker ,  Anne Schoendorf ,  Mark R. Wildung

    IPC: C12Q001/68 ,  C07H021/04 ,  C12N009/10 ,  C12P021/02 ,  C12N005/06

    CPC classification number: C12P17/02 ,  C12N9/1029

    Abstract: Transacylase enzymes and the use of such enzymes to produce paclitaxel and related taxoids, as well as intermediates in the paclitaxel biosynthetic pathway, are disclosed. Also disclosed are nucleic acid sequences encoding such transacylase enzymes such as (but without limitation) C-13 phenylpropanoid side chain-CoA acyltransferase and benzoyl-CoA:3null-N-debenzoyl-2null-deoxytaxol N-benzoyltransferase.

    Abstract translation: 公开了转酰胺酶和这种酶在紫杉醇生物合成途径中产生紫杉醇和相关紫杉烷以及中间体的用途。 还公开了编码这样的转酰酶的核酸序列，例如（但不限于）C-13苯丙素侧链-CoA酰基转移酶和苯甲酰辅酶A：3'-N-脱苯甲酰基-2'-脱氧塔考酚N-苯甲酰基转移酶。

    Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-beta-farnesene
    36.
    发明申请
    Isolation and bacterial expression of a sesquiterpene synthase cDNA clone from peppermint (Mentha x piperita, L.) that produces the aphid alarm pheromone (E)-beta-farnesene 失效
    从薄荷（Mentha x piperita，L.）中产生蚜虫报警信息素（E） - 法呢烯的倍半萜合酶cDNA克隆的分离和细菌表达

    公开(公告)号：US20030166204A1

    公开(公告)日：2003-09-04

    申请号：US09860282

    申请日：2001-05-17

    Applicant: Washington State University Research Foundation

    Inventor： Rodney Bruce Croteau ,  John E. Crock

    IPC: C12N009/10 ,  C07H021/04 ,  C12P021/02 ,  C12N005/04

    CPC classification number: C12P5/007 ,  C12N9/88

    Abstract: A cDNA encoding (E)-null-farnesene synthase from peppermint (Mentha piperita) has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Accordingly, an isolated DNA sequence (SEQ ID NO:1) is provided which codes for the expression of (E)-null-farnesene synthase (SEQ ID NO:2), from peppermint (Mentha piperisa). In other aspects, replicable recombinant cloning vehicles are provided which code for (E)-null-farnesene synthase, or for a base sequence sufficiently complementary to at least a portion of (E)-null-farnesene synthase DNA or RNA to enable hybridization therewith. In yet other aspects, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding (E)-null-farnesene synthase. Thus, systems and methods are provided for the recombinant expression of the aforementioned recombinant (E)-null-famesene synthase that may be used to facilitate its production, isolation and purification in significant amounts. Recombinant (E)-null-farnesene synthase may be used to obtain expression or enhanced expression of (E)-null-famesene synthase in plants in order to enhance the production of (E)-null-farnesene, or may be otherwise employed for the regulation or expression of (E)-null-farnesene synthase, or the production of its product.

    Abstract translation: 已经分离了来自薄荷（薄荷胡椒）的编码（E） - 法呢烯合成酶的cDNA并测序，并确定了相应的氨基酸序列。 因此，提供了从薄荷（Mentha piperisa）编码（E） - 法呢烯合成酶（SEQ ID NO：2）的表达的分离的DNA序列（SEQ ID NO：1）。 在其它方面，提供了编码（E） - 法呢烯合成酶的可重复的重组克隆载体，或与（E） - 法呢烯合成酶DNA或RNA的至少一部分充分互补的碱基序列，以使其能够与其进行杂交 。 在另一方面，提供已经用编码（E） - 法呢烯合成酶的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 因此，提供了用于重组表达上述重组（E）-β-甲酰基合成酶的系统和方法，其可用于促进其显着量的产生，分离和纯化。 可以使用重组（E） - 法呢烯合成酶来获得植物中（E）-β-fameene合酶的表达或增强的表达，以增强（E） - 法呢烯的生产，或者可以另外用于 （E） - 法呢烯合成酶的调节或表达，或其产物的生产。

    Virtual assembly design environment (VADE)
    39.
    发明申请
    Virtual assembly design environment (VADE) 审中-公开
    虚拟装配设计环境（VADE）

    公开(公告)号：US20020123812A1

    公开(公告)日：2002-09-05

    申请号：US09888055

    申请日：2001-06-21

    Applicant: Washington State University Research Foundation.

    Inventor： Sankar Jayaram ,  Uma Jayaram ,  Yong Wang ,  Hrishikesh Tirumali ,  Hiral Chandrana ,  Hugh Ingram Connacher ,  Kevin Lyons ,  Peter Fred Hart

    IPC: G06F019/00 ,  G06K009/00 ,  G06T015/00 ,  G06T017/00 ,  G03B021/60 ,  G06T013/00 ,  G06T015/70

    CPC classification number: G06T19/20 ,  G06T2210/21 ,  G06T2219/2004 ,  G06T2219/2008

    Abstract: A virtual assembly design environment that simulates axial and planar constrained motion for multiple parts in any combination and application order. Dynamic simulation methods are used to simulate object behavior in the design environment using physical laws and collision detection algorithms. The physical properties, e.g., mass properties, of parts in an assembly are created in a separate CAD system. In one embodiment, physical property information is transferred from the CAD system to the virtual reality environment where it is used in dynamic simulations. The parts behave realistically in a user's hand, constrained on the base part, or moving freely in space. A swept volume can be generated directly in the CAD system. Real-time bi-directional data transfer between a VR environment and the CAD system is also provided. As a result, the user can perform parametric design modifications in the virtual environment by the use of a CAD system.

    Abstract translation: 虚拟装配设计环境，以任何组合和应用顺序模拟多个零件的轴向和平面约束运动。 动态仿真方法用于使用物理定律和碰撞检测算法来模拟设计环境中的对象行为。 组件中部件的物理性质，例如质量特性是在单独的CAD系统中产生的。 在一个实施例中，物理属性信息从CAD系统传送到在动态模拟中使用的虚拟现实环境。 这些部件在用户手中实际操作，限制在基部上，或在空间中自由移动。 扫描体积可以直接在CAD系统中生成。 还提供了VR环境和CAD系统之间的实时双向数据传输。 因此，用户可以通过使用CAD系统在虚拟环境中执行参数设计修改。

    Geranyl diphosphate synthase large subunit, and methods of use
    40.
    发明申请
    Geranyl diphosphate synthase large subunit, and methods of use 审中-公开
    香叶基二磷酸合成酶大亚基，以及使用方法

    公开(公告)号：US20020106772A1

    公开(公告)日：2002-08-08

    申请号：US09934778

    申请日：2001-08-21

    Applicant: Washington State University Research Foundation

    Inventor： Rodney B. Croteau ,  Charles C. Burke

    IPC: C12N009/16 ,  C07H021/04

    CPC classification number: C12N9/1085 ,  A61K48/00 ,  C12N15/8243

    Abstract: A cDNA encoding geranyl diphosphate synthase large subunit from peppermint has been isolated and sequenced, and the corresponding amino acid sequence has been determined. Replicable recombinant cloning vehicles are provided which code for geranyl diphosphate synthase large subunit). In another aspect, modified host cells are provided that have been transformed, transfected, infected and/or injected with a recombinant cloning vehicle and/or DNA sequence encoding geranyl diphosphate synthase large subunit. In yet another aspect, the present invention provides isolated, recombinant geranyl diphosphate synthase protein comprising an isolated, recombinant geranyl diphosphate synthase large subunit protein and an isolated, recombinant geranyl diphosphate synthase small subunit protein. Thus, systems and methods are provided for the recombinant expression of geranyl diphosphate synthase.

    Abstract translation: 已经分离并测序了从薄荷中编码香叶基二磷酸合酶大亚基的cDNA，并测定了相应的氨基酸序列。 提供可复制的重组克隆载体，其编码香叶基二磷酸合酶大亚基）。 在另一方面，提供已经用编码香叶基二磷酸合成酶大亚基的重组克隆载体和/或DNA序列转化，转染，感染和/或注射的修饰的宿主细胞。 在另一方面，本发明提供了分离的重组香叶基二磷酸合酶蛋白，其包含分离的重组香叶基二磷酸合酶大亚基蛋白和分离的重组香叶基二磷酸合酶小亚基蛋白。 因此，提供了重组表达香叶基二磷酸合成酶的系统和方法。

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