公开(公告)号：US20210002721A1

公开(公告)日：2021-01-07

申请号：US16844141

申请日：2020-04-09

Applicant: 10x Genomics, Inc.

Inventor： Christopher Hindson ,  Michael Schnall-Levin ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20200377942A1

公开(公告)日：2020-12-03

申请号：US16998414

申请日：2020-08-20

Applicant: 10X GENOMICS, INC.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov ,  Paul Hardenbol ,  Rajiv Bharadwaj ,  Xinying Zheng ,  Phillip Belgrader

IPC: C12Q1/6874 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6869

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing. Such polynucleotide processing may be useful for a variety of applications, including polynucleotide sequencing.

公开(公告)号：US20200255894A1

公开(公告)日：2020-08-13

申请号：US16736323

申请日：2020-01-07

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Serge Saxonov

IPC: C12Q1/6874 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US10590244B2

公开(公告)日：2020-03-17

申请号：US16178430

申请日：2018-11-01

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Christopher Hindson ,  Adam Lowe ,  Andrew D. Price

IPC: C08J3/075 ,  C08J3/24 ,  B01L3/00

Abstract: The present disclosure provides systems and methods for making a hydrogel comprising a cell, cell nucleus, or one or more components derived from a cell or cell nucleus. A method for making a hydrogel may comprise providing a cell or cell nucleus, a first polymer, wherein the first polymer comprises a plurality of first crosslink precursors, each of the plurality of first crosslink precursors comprising an azide group; providing a second polymer, wherein the second polymer comprises a plurality of second crosslink precursors, each of the plurality of second crosslink precursors comprising an alkyne group; and crosslinking the first polymer and the second polymer via a reaction between a first section of the first crosslink precursors and a second section of the second crosslink precursors, thereby providing the hydrogel comprising the cell or cell nucleus.

公开(公告)号：US20200002763A1

公开(公告)日：2020-01-02

申请号：US16439675

申请日：2019-06-12

Applicant: 10X Genomics, Inc.

Inventor： Phillip Belgrader ,  Zachary Bent ,  Rajiv Bharadwaj ,  Vijay Kumar Sreenivasa Gopalan ,  Josephine Harada ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Michael Ybarra Lucero ,  Geoffrey McDermott ,  Elliott Meer ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Serge Saxonov ,  John R. Stuelpnagel ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Indira Wu ,  Solongo Batjargal Ziraldo ,  Stephane Claude Boutet ,  Sarah Taylor ,  Niranjan Srinivas

IPC: C12Q1/6874 ,  C12Q1/683 ,  C12Q1/6806

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization.

公开(公告)号：US20190383804A1

公开(公告)日：2019-12-19

申请号：US16375119

申请日：2019-04-04

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC: G01N33/53 ,  G01N33/543 ,  C12N15/11

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, epigenetic information (e.g., accessible chromatin, DNA methylation), and RNA molecules (e.g., mRNA or CRISPR guide RNAs). In some cases, the disclosed methods comprise analysis of analytes from a cell using a cell bead.

公开(公告)号：US20190382836A1

公开(公告)日：2019-12-19

申请号：US16395090

申请日：2019-04-25

Applicant: 10X GENOMICS, INC.

Inventor： Christopher Hindson ,  Michael Schnall-Levin ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US20190367969A1

公开(公告)日：2019-12-05

申请号：US16375093

申请日：2019-04-04

Applicant: 10X Genomics, Inc.

Inventor： Kamila Belhocine ,  Jason Bell ,  Zachary Bent ,  Rajiv Bharadwaj ,  Christopher Hindson ,  Mohammad Rahimi Lenji ,  Bill Kengli Lin ,  Anthony Makarewicz ,  Geoffrey McDermott ,  Elliott Meer ,  Francesca Meschi ,  Tarjei Sigurd Mikkelsen ,  Christopher Joachim O'Keeffe ,  Katherine Pfeiffer ,  Andrew D. Price ,  Paul Ryvkin ,  Michael Schnall-Levin ,  Sarah Taylor ,  Jessica Michele Terry ,  Tobias Daniel Wheeler ,  Yifeng Yin ,  Xinying Zheng ,  Solongo Batjargal Ziraldo ,  Eswar Prasad Ramachandran Iyer ,  Luigi Jhon Alvarado Martinez

IPC: C12Q1/6818 ,  C12Q1/6827

Abstract: The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization from a single cell. Such polynucleotide processing may be useful for a variety of applications. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins) and chromatin (e.g., accessible chromatin).

公开(公告)号：US20190203262A1

公开(公告)日：2019-07-04

申请号：US16242962

申请日：2019-01-08

Applicant: 10X Genomics, Inc.

Inventor： Benjamin Hindson ,  Christopher Hindson ,  Michael Schnall-Levin ,  Kevin Ness ,  Mirna Jarosz ,  Donald Masquelier ,  Serge Saxonov ,  Landon Merrill ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/6806 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00572 ,  B01J2219/00722 ,  C12N15/1006 ,  C12N15/1065 ,  C12Q2535/122 ,  C12Q2563/159

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.

公开(公告)号：US10323279B2

公开(公告)日：2019-06-18

申请号：US16052486

申请日：2018-08-01

Applicant: 10X GENOMICS, INC.

Inventor： Christopher Hindson ,  Michael Schnall-Levin ,  Andrew Price ,  Paul Hardenbol ,  Yuan Li

IPC: C12Q1/68 ,  C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6855 ,  C12Q1/6869

Abstract: This disclosure provides methods and compositions for sample processing, particularly for sequencing applications. Included within this disclosure are bead compositions, such as diverse libraries of beads attached to large numbers of oligonucleotides containing barcodes. Often, the beads provides herein are degradable. For example, they may contain disulfide bonds that are susceptible to reducing agents. The methods provided herein include methods of making libraries of barcoded beads as well as methods of combining the beads with a sample, such as by using a microfluidic device.