HIGH FRUCTAN CEREAL PLANTS
    32.
    发明申请
    HIGH FRUCTAN CEREAL PLANTS 有权
    高科技谷物植物

    公开(公告)号:US20110281818A1

    公开(公告)日:2011-11-17

    申请号:US13008746

    申请日:2011-01-18

    摘要: The invention provides cereal plants having a high level of fructan useful for the production of a range of food, beverage, nutraceutical and pharmaceutical products. The invention provides methods of producing high-fructan products from plants modified to comprise a reduced level of an endogenous polypeptide with starch synthase activity, and products so produced. In some embodiments, plants are modified by introduction of an agent such as a nucleic acid molecule which down regulates endogenous starch synthase II gene expression.

    摘要翻译: 本发明提供了具有高水平的果聚糖的谷类植物,其用于生产一系列食品,饮料,营养药物和药物产品。 本发明提供了从修饰为包含具有淀粉合成酶活性的内源性多肽的降低水平的植物生产高果聚糖产物的方法以及如此制备的产品。 在一些实施方案中,通过引入下调调节内源淀粉合成酶II基因表达的核酸分子的试剂来修饰植物。

    PLANTS WITH MODIFIED STARCH METABOLISM
    33.
    发明申请
    PLANTS WITH MODIFIED STARCH METABOLISM 审中-公开
    具有改性淀粉代谢的植物

    公开(公告)号:US20110045127A1

    公开(公告)日:2011-02-24

    申请号:US12745237

    申请日:2008-11-27

    摘要: The specification provides methods of obtaining a genetically modified plant which has increased production potential compared to a control plant, the method comprising the steps of i) obtaining a plurality of plants at least one of which comprises in its genome a heterologous polynucleotide, ii) identifying from the plurality of plants a plant which has increased production potential relative to the control plant and comprises the heterologous polynucleotide, and iii) selecting the genetically modified plant, wherein the polynucleotide comprises a transcriptional control sequence operably linked to a nucleic acid sequence which encodes an agent that modifies endogenous starch phosphorylation and/or starch degradation in the plant. In some embodiments, the plant has increased endogenous glycosylase or increased digestibility compared to a control plant. In some specific embodiments, the endogenous starch phosphorylation and/or starch degradation is modified by modifying expression or activity of one or more enzymes selected from the group consisting of α-amylase (EC 3.2.1.1), β-amylase (EC 3.2.1.2), glucoamylase (EC 3.2.1.3), starch phosphorylase (EC2.4.1.1), glycosylase (EC 3.1.33), sucrase-isomaltase (EC 3.2.10), amylomaltase (EC 2.4.1.25), maltase (EC 3.2.1.20), isoamylase, and α-glucan, water dikinase (GWD, EC 2.7.9.4).

    摘要翻译: 本说明书提供了获得与对照植物相比具有增加的生产潜力的遗传修饰植物的方法,所述方法包括以下步骤:i)获得多个植物,其中至少一个在其基因组中包含异源多核苷酸,ii)鉴定 来自多个植物的植物相对于对照植物具有增加的生产潜力并且包含异源多核苷酸,以及iii)选择遗传修饰植物,其中所述多核苷酸包含可操作地连接到编码 修饰植物内源性淀粉磷酸化和/或淀粉降解的试剂。 在一些实施方案中,与对照植物相比,植物具有增加的内源糖基化酶或增加的消化率。 在一些具体实施方案中,内源性淀粉磷酸化和/或淀粉降解通过修饰选自以下的一种或多种酶的表达或活性来修饰:α-淀粉酶(EC 3.2.1.1),淀粉酶(EC 3.2。 1.2),葡糖淀粉酶(EC 3.2.1.3),淀粉磷酸化酶(EC2.4.1.1),糖基化酶(EC 3.1.33),蔗糖酶异麦芽糖酶(EC 3.2.10),淀粉麦芽糖酶(EC 2.4.1.25),麦芽糖酶 3.2.1.20),异淀粉酶和α-葡聚糖,水二激酶(GWD,EC 2.7.9.4)。

    Genes encoding wheat starch synthases and uses thereof
    39.
    发明授权
    Genes encoding wheat starch synthases and uses thereof 有权
    编码小麦淀粉合成酶的基因及其用途

    公开(公告)号:US07001771B1

    公开(公告)日:2006-02-21

    申请号:US10018418

    申请日:2000-04-28

    摘要: The present invention provides isolated nucleic acid molecules encoding wheat starch syntheses, and probes and primers derived therefrom, which are useful in the modificaiton of plant starch content and/or composition, and for screening plant lines to determine the presence of natural and/or induced mutations in starch synthase genes which affect starch content and/or composition. More particularly the isolated nucleic acid molecules of the present invention further provide for the screening-assisted breeding of plants having desirable starch content and/or composition, in addition to providing for the direct genetic manipulation of plant starch content and/or composition.

    摘要翻译: 本发明提供编码小麦淀粉合成的分离的核酸分子,以及衍生自其的探针和引物,其可用于植物淀粉含量和/或组合物的改良,并用于筛选植物系以确定天然和/或诱导的 影响淀粉含量和/或组成的淀粉合成酶基因中的突变。 更具体地,除了提供植物淀粉含量和/或组合物的直接遗传操作之外,本发明的分离的核酸分子进一步提供具有所需淀粉含量和/或组成的植物的筛选辅助育种。