公开(公告)号：US09023632B2

公开(公告)日：2015-05-05

申请号：US14150206

申请日：2014-01-08

申请人： BASF SE

发明人： Hartwig Schröder ,  Stefan Haefner ,  Gregory Von Abendroth ,  Rajan Hollmann ,  Aline Raddatz ,  Hansgeorg Ernst ,  Hans Gurski

IPC分类号： C12P7/46 ,  C12P7/44 ,  C12N1/32 ,  C12N9/10 ,  C12R1/01

CPC分类号： C12P7/46 ,  C12N1/32 ,  C12N9/1029 ,  C12R1/01 ,  C12Y203/01054

摘要： The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.

摘要翻译： 本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及 涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。 本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。

公开(公告)号：US20140011243A1

公开(公告)日：2014-01-09

申请号：US13935043

申请日：2013-07-03

申请人： Wintershall Holding GmbH ,  BASF SE

发明人： Beata Brockmann ,  Andrea Herold ,  Oskar Zelder ,  Stefan Haefner ,  Christian Fleck ,  Hartwig Schröder ,  Mari Granström ,  Julia Kristiane Schmidt

IPC分类号： C12P19/04 ,  C12N15/80

CPC分类号： C12P19/04 ,  C08B37/0024 ,  C08L5/00 ,  C12N9/1051 ,  C12N15/80 ,  C12P19/18 ,  C12Y204/01034 ,  Y02P20/52

摘要： The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.

摘要翻译： 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于所述遗传修饰的微生物过表达（i）编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸和/或（ii ）具有1,3-β-D-葡聚糖合酶活性的多肽，与相同菌株的相应未修饰的对照微生物相比较。 本发明还涉及编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于产生β-葡聚糖的用途。 此外，本发明涉及生产β-葡聚糖的方法，包括在编码具有1,3-β-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加所述多核苷酸的表达，或多核苷酸 将具有1,3-β-D-葡聚糖合酶活性的多肽编码成能够合成β-葡聚糖的微生物。

公开(公告)号：US08557555B2

公开(公告)日：2013-10-15

申请号：US13689958

申请日：2012-11-30

申请人： BASF SE

发明人： Stefan Haefner ,  Annegret Welzel ,  Robert Thummer

IPC分类号： C12N9/00 ,  C12N9/16 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/16 ,  C07K2319/21

摘要： The invention relates to a synthetic phytase with elevated thermostability, elevated stability to acids at pH 2, elevated stability to pepsin and with a broadened active pH range, and to an isolated nucleic acid sequence coding for a synthetic phytase and to the use of the phytase in an animal feed for reducing the phosphate content in the slurry and to animal feed additives and animal feeds comprising the synthetic phytase.

摘要翻译： 本发明涉及具有升高的热稳定性的合成植酸酶，在pH2下对酸的稳定性升高，对胃蛋白酶的稳定性升高，并且具有扩大的活性pH范围，以及编码合成植酸酶的分离的核酸序列和植酸酶的使用 在用于降低浆料中的磷酸盐含量的动物饲料和包含合成植酸酶的动物饲料添加剂和动物饲料中。