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公开(公告)号：US11994459B2
公开(公告)日：2024-05-28
申请号：US18130571
申请日：2023-04-04
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Joseph T. Trotter , Keegan Owsley
IPC: G01N15/14 , B07C5/342 , B07C5/36 , G01N15/1434 , G01N15/149 , G06V10/70 , G06V20/69 , G16C20/20
CPC classification number: G01N15/1459 , B07C5/3425 , B07C5/361 , G01N15/1425 , G01N15/1434 , G06V10/87 , G06V20/695 , G06V20/698 , G16C20/20 , G01N15/149
Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.
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公开(公告)号：US20230333002A1
公开(公告)日：2023-10-19
申请号：US18214317
申请日：2023-06-26
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
CPC classification number: G01N15/1434 , G01N15/1475 , G01N15/1425 , G01N15/147 , G01N15/1429 , G01N21/645 , G01N15/14 , G02B21/06 , G01N2015/1006 , G01N2015/1454 , G01N2021/6482 , G01N2015/1447
Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号：US20230057188A1
公开(公告)日：2023-02-23
申请号：US17955060
申请日：2022-09-28
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Jonathan Lin , Matthew Bahr , Keegan Owsley
Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.
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公开(公告)号：US11543295B2
公开(公告)日：2023-01-03
申请号：US17225730
申请日：2021-04-08
Applicant: Becton, Dickinson and Company
Inventor： Peter Mage , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for spectrally resolving light from fluorophores having overlapping fluorescence spectra in a sample. Methods according to certain embodiments include detecting light with a light detection system from a sample having a plurality of fluorophores having overlapping fluorescence spectra and spectrally resolving light from each fluorophore in the sample. In some embodiments, methods include estimating the abundance of one or more of the fluorophores in the sample, such as on a particle. In certain instances, methods include identifying the particle in the sample based on the abundance of each fluorophore and sorting the particle. Methods according to some embodiments includes spectrally resolving the light from each fluorophore by calculating a spectral unmixing matrix for the fluorescence spectra of each fluorophore. Systems and integrated circuit devices (e.g., a field programmable gate array) for practicing the subject methods are also provided.
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公开(公告)号：US20220317018A1
公开(公告)日：2022-10-06
申请号：US17845294
申请日：2022-06-21
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , David A. Roberts
Abstract: Disclosed herein include systems, devices, computer readable media, and methods for subsampling flow cytometric event data. First and second flow cytometric event data can be transformed into a lower-dimensional space, associated with a plurality of bins, and assigned to a first bin and a second bin. Subsampled flow cytometric event data comprising the first flow cytometric event data can be generated. The subsampled flow cytometric event data can comprise the second flow cytometric event data if the first bin and the second bin are different. The subsampled flow cytometric event data may not comprise the second flow cytometric event data if the first bin and the second bin are identical.
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Patent Agency Ranking
36.发明申请公开(公告)号：US20220236164A1
公开(公告)日：2022-07-28
申请号：US17545646
申请日：2021-12-08
Applicant: Becton, Dickinson and Company
Inventor： Matthew Bahr , Christopher Ghazi , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for determining drop delay of a particle in a flow stream (e.g., in a particle analyzer). Methods according to certain embodiments include irradiating the particle in the flow stream with two or more spatially separated lasers, detecting light from the particle in a first photodetector channel and a second photodetector channel calculating a velocity of the particle in the flow stream based on the detected light in the first photodetector channel and the second photodetector channel and determining the drop delay of the particle based on the calculated velocity. Systems (e.g., particle analyzers) having a light source with two or more spatially separated lasers and a light detection system for practicing the subject methods are also described. Integrated circuits and non-transitory computer readable storage medium having instructions stored thereon for determining drop delay according to the subject methods are also provided.
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37.发明授权公开(公告)号：US11328422B2
公开(公告)日：2022-05-10
申请号：US16817135
申请日：2020-03-12
Applicant: Becton, Dickinson and Company
Inventor： Matthew Bahr , Keegan Owsley
Abstract: Disclosed herein include embodiments of a system, a device, and a method for sorting a plurality cells of a sample. A plurality of raw images comprising pixels of complex values in a frequency space can be generated from a plurality of channels of fluorescence intensity data of fluorescence emissions of fluorophores, the fluorescence emissions being elicited by fluorescence imaging using radiofrequency-multiplexed excitation in a temporal space. Spectral unmixing can be performed on the raw images prior to a sorting decision being made.
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公开(公告)号：US11327003B2
公开(公告)日：2022-05-10
申请号：US16557539
申请日：2019-08-30
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Jonathan Lin , Joseph Trotter , Keegan Owsley
Abstract: Non-parametric transforms such as t-distributed stochastic neighbor embedding (tSNE) are used to analyze multi-parametric data such as data derived from flow cytometry or other particle analysis systems and methods. These transforms may be included for dimensionality reduction and identification of subpopulations (e.g., gating). By nature, non-parametric transforms cannot transform new observations without training a new transformation based on the entire dataset including the new observations. The features described parameterize non-parametric transforms using a neural network thereby allowing a small training dataset to be transformed using non-parametric techniques. The training dataset may then be used to generate an accurate parametric model for assessing additional events in a manner consistent with the initial events.
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公开(公告)号：US20200378882A1
公开(公告)日：2020-12-03
申请号：US16887538
申请日：2020-05-29
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US20200309664A1
公开(公告)日：2020-10-01
申请号：US16817331
申请日：2020-03-12
Applicant: Becton, Dickinson and Company
Inventor： Matthew Bahr , Eric D. Diebold , Jonathan Lin , Keegan Owsley
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image of an object in the flow stream in an interrogation region and determining whether the object in the flow stream is an aggregate based on the generated image. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image of an object in a flow stream and to determine whether the object is an aggregate are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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