公开(公告)号：US10889793B2

公开(公告)日：2021-01-12

申请号：US15639120

申请日：2017-06-30

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Sol M. Resnick ,  Drew D. Regitsky

IPC: C12M1/02 ,  C12M1/04 ,  C12M1/08 ,  C12P5/02 ,  C12P7/16 ,  C12P7/18 ,  C12P7/40 ,  C12P7/42 ,  C12P17/02 ,  C12N1/08 ,  C12N1/10 ,  C12N1/12 ,  C12N1/20 ,  C12N13/00 ,  C02F3/32 ,  A01G33/00 ,  C12M1/00 ,  C12M1/12

Abstract: A fermenter can have at least one hollow fluid conduit disposed at least partially within a vessel. An external circumference of the hollow fluid conduit and an interior circumference of the vessel can define a downward flow path through which a multi-phase mixture including a liquid media and compressed gas substrate bubbles flows. An interior circumference of the hollow fluid conduit can defined an upward flow path which is in fluid communication with the downward flow path. The multi-phase liquid can flow through the upward flow path and exit the fermenter. Cooling may be provided in the hollow fluid conduit or the vessel. One or more backpressor generators can be used to maintain a backpressure on the fermenter. One or more fluid movers can be used to variously create an induced and/or forced flow in the downward and upward flow paths.

公开(公告)号：US10450548B2

公开(公告)日：2019-10-22

申请号：US14898948

申请日：2014-06-18

Applicant: CALYSTA, INC.

Inventor： Renee M. Saville ,  Sungwon Lee ,  Drew D. Regitsky ,  Sol M. Resnick ,  Joshua A. Silverman

IPC: C12N1/20 ,  C12N9/04 ,  C12P7/56 ,  C07B59/00 ,  C07C59/08 ,  C12N15/74

Abstract: The present disclosure provides non-naturally occurring c1 microorganisms useful for the production of lactate and related compositions, as well as methods for the biologically production of lactate. In specific embodiments, the present disclosure provides non-naturally occurring methanotrophic bacteria which are useful for producing lactate from c1 substrates.

公开(公告)号：US20190271019A1

公开(公告)日：2019-09-05

申请号：US16413422

申请日：2019-05-15

Applicant: CALYSTA, INC.

Inventor： Joshua A. Silverman ,  Lorraine Joan Giver ,  Jana Mueller ,  Renee M. Saville ,  Drew D. Regitsky

IPC: C12P19/18 ,  C12N9/00 ,  A23K50/00 ,  A23K10/12 ,  A23K20/163 ,  C12P19/04 ,  C12N9/10

Abstract: The present disclosure relates to recombinant microorganisms engineered for enhanced production of a desired carbohydrate, as well as related biomass, and compositions which are useful, inter alia, as animal feed ingredients. The present disclosure also provides related methods.

公开(公告)号：US20190264169A1

公开(公告)日：2019-08-29

申请号：US16299772

申请日：2019-03-12

Applicant: CALYSTA, INC.

Inventor： Howard Lam Ho Fong ,  John H Grate ,  Luan Nguyen ,  Joshua A. Silverman ,  Lisa Marie Newman ,  Lorraine Joan Giver ,  Drew D. Regitsky

IPC: C12N1/20 ,  C10L1/04 ,  C12N15/74 ,  C10L3/10 ,  B01D53/85 ,  A23K10/10 ,  C10L1/08 ,  C10L1/06 ,  C12R1/01 ,  C10G3/00 ,  A23N17/00 ,  C12N9/02 ,  C12P7/64

Abstract: The present disclosure provides compositions and methods for using recombinant C1 metabolizing microorganisms capable of metabolizing sulfur containing compounds and other contaminants to biologically convert sour or acidic natural gas into high-value molecules, and to allow recovery of stranded oil.

公开(公告)号：US20190264164A1

公开(公告)日：2019-08-29

申请号：US16405587

申请日：2019-05-07

Applicant: Calysta, Inc.

Inventor： Luan Thanh Nguyen ,  Arild Johannessen ,  Graham Ian Aylen ,  Joshua A. Silverman

IPC: C12M1/00 ,  C12M1/34 ,  C12M1/42 ,  C12N1/30

Abstract: Reactors, systems and processes for the production of biomass by culturing microorganisms in aqueous liquid culture medium circulating inner loop reactor which utilize nonvertical pressure reduction zones are described. Recovery and processing of the culture microorganisms to obtain products, such as proteins or hydrocarbons is described.

公开(公告)号：US20180282769A1

公开(公告)日：2018-10-04

申请号：US15877747

申请日：2018-01-23

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman

IPC: C12P7/64 ,  C12P7/42 ,  C12N9/02 ,  C12N9/06 ,  C12N9/16 ,  C12P7/44 ,  C12N9/04 ,  C12P7/24 ,  C12N15/52 ,  C12N9/00

CPC classification number: C12P7/6409 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0026 ,  C12N9/0077 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12P7/64 ,  C12P7/6436 ,  C12Y101/01 ,  C12Y101/01001 ,  C12Y102/99006 ,  C12Y301/02 ,  C12Y602/01

Abstract: The present disclosure provides compositions and methods for biologically producing fatty acid derivatives, such as fatty alcohols, from recombinant C1 metabolizing microorganisms that utilize C1 substrates such as methane or natural gas as a feedstock.

公开(公告)号：US09909153B2

公开(公告)日：2018-03-06

申请号：US14441153

申请日：2013-11-08

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman

IPC: C12P7/64 ,  C12N9/00 ,  C12N9/16 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12N9/04 ,  C12N9/02 ,  C12N9/06

CPC classification number: C12P7/6409 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0026 ,  C12N9/0077 ,  C12N9/16 ,  C12N9/93 ,  C12N15/52 ,  C12P7/24 ,  C12P7/42 ,  C12P7/44 ,  C12P7/64 ,  C12P7/6436 ,  C12Y101/01 ,  C12Y101/01001 ,  C12Y102/99006 ,  C12Y301/02 ,  C12Y602/01

Abstract: The present disclosure provides compositions and methods for biologically producing fatty acid derivatives, such as fatty alcohols, from recombinant C1 metabolizing microorganisms that utilize C1 substrates such as methane or natural gas as a feedstock.

公开(公告)号：US20170306358A1

公开(公告)日：2017-10-26

申请号：US15637838

申请日：2017-06-29

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Thomas Joseph Purcell

IPC: C12P5/02 ,  C12N1/20

CPC classification number: C12P5/026 ,  C12N1/20 ,  C12N9/16 ,  C12N9/88 ,  Y02E50/343

Abstract: The present disclosure relates to biosynthetic methods for producing propylene from C1 substrates (e.g., methane, methanol, carbon monoxide, syngas) and to genetically engineered organisms having propylene biosynthesis capability, as well as engineered organisms having a butyrate/butanol-producing pathway.

公开(公告)号：US20160333384A1

公开(公告)日：2016-11-17

申请号：US15111733

申请日：2015-01-16

Applicant: CALYSTA, INC.

Inventor： Joshua A. Silverman ,  Lorraine Joan Giver ,  Jana Mueller ,  Renee M. Saville ,  Drew D. Regitsky

IPC: C12P19/18 ,  A23K10/12 ,  A23K20/163 ,  C12N9/10 ,  C12P19/04

CPC classification number: C12P19/18 ,  A23K10/12 ,  A23K20/163 ,  A23K50/00 ,  C12N9/00 ,  C12N9/1051 ,  C12P19/04 ,  C12Y204/01034

Abstract: The present disclosure relates to recombinant microorganisms engineered for enhanced production of a desired carbohydrate, as well as related biomass, and compositions which are useful, inter alia, as animal feed ingredients. The present disclosure also provides related methods.

Abstract translation: 本公开涉及用于增强生产所需碳水化合物以及相关生物质的工程改造的重组微生物，以及尤其可用作动物饲料成分的组合物。 本公开还提供了相关方法。

公开(公告)号：US20200205440A1

公开(公告)日：2020-07-02

申请号：US16634114

申请日：2018-08-16

Applicant: Calysta, Inc.

Inventor： Joshua A. Silverman ,  Sungwon Lee

IPC: A23K10/16 ,  A23K20/158 ,  A23K50/80 ,  A23K50/75 ,  A23K10/30

Abstract: The present disclosure provides methods for improving accumulation of one or more polyunsaturated fatty acids in an animal through the use of a boosted feed composition comprising polyunsaturated fatty acids and a bacterial biomass (e.g., methylotrophic, methanotrophic, or both), wherein the animal that is fed and consumes the boosted feed composition accumulates the one or more polyunsaturated fatty acids more efficiently than when fed a reference feed composition lacking the bacterial biomass.