公开(公告)号：US07897257B2

公开(公告)日：2011-03-01

申请号：US11911540

申请日：2006-04-13

申请人： Mathias Alterman ,  Andreas Axen ,  Gunnar Glad ,  Dag Lindstrom ,  Ulrika Meyer ,  Nils Norrman ,  Tobias Soderman

发明人： Mathias Alterman ,  Andreas Axen ,  Gunnar Glad ,  Dag Lindstrom ,  Ulrika Meyer ,  Nils Norrman ,  Tobias Soderman

IPC分类号： B32B5/16 ,  B05D7/00

CPC分类号： G01N33/54326 ,  H01F1/0054 ,  H01F1/36 ,  Y10T428/12014 ,  Y10T428/12028 ,  Y10T428/2998

摘要： The present invention relates to magnetic beads suitable for, for example, isolation of proteins, cells, and viruses and also for diagnostic applications and cell cultivation. The magnetic beads are composite beads with an inner core of metal particles, which are coated with an inert synthetic polymer and these are then enclosed in a hydrophilic porous polymer, preferably agarose. This provides porous biocompatible beads without metal leakage. The beads may be used for cell cultivation or for chromatography. When the beads are used for chromatography the agarose layer is preferably provided with ligands having affinity for selected biomolecules.

摘要翻译： 本发明涉及适合于例如分离蛋白质，细胞和病毒以及用于诊断应用和细胞培养的磁珠。 磁珠是具有金属颗粒内核的复合珠粒，其中涂有惰性合成聚合物，然后将其包封在亲水性多孔聚合物，优选琼脂糖中。 这提供了多孔的生物相容珠，而没有金属泄漏。 珠粒可用于细胞培养或色谱法。 当珠子用于层析时，琼脂糖层优选具有对选定的生物分子具有亲和性的配体。

公开(公告)号：US20080237124A1

公开(公告)日：2008-10-02

申请号：US11996357

申请日：2006-08-30

申请人： Andreas Axen ,  Gunnar Glad ,  Henrik Ihre ,  Anders Larsson ,  Nils Norrman

发明人： Andreas Axen ,  Gunnar Glad ,  Henrik Ihre ,  Anders Larsson ,  Nils Norrman

IPC分类号： B01D15/08 ,  B01J39/26

CPC分类号： B01J39/26 ,  B01D15/362 ,  B01J20/289 ,  B01J39/19

摘要： The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

摘要翻译： 本发明涉及一种制备色谱基质的方法，包括提供包含可用羟基的多糖载体; 并使所述羟基与乙烯基磺酸酯反应以提供磺酸盐官能化的（S-官能化的）阳离子交换剂。 载体的羟基可以是琼脂糖聚合物的羟基; 或者它们可以提供在增量剂上，例如多羟基官能聚合物。 在一个实施方案中，载体由具有改进的流动压力性质的琼脂糖制成。

公开(公告)号：US20080199959A1

公开(公告)日：2008-08-21

申请号：US11917169

申请日：2006-06-19

申请人： Mattias Algotsson ,  Gunnar Glad ,  Nicolas Thevenin

发明人： Mattias Algotsson ,  Gunnar Glad ,  Nicolas Thevenin

IPC分类号： C12N5/06 ,  C12N1/20 ,  C12N1/00 ,  C12M1/00

CPC分类号： C12N5/0075 ,  G01N33/5005 ,  G01N33/5432

摘要： The present invention relates to a method for cell culture, more precisely small scale cell culture. In the present invention a screening tool is used which comprises particulate matter or microcarriers, such as beads, attached to a solid support, such as a microtiter plate, for the cultivation of cells on said microcarriers. The microcarriers are preferably cultivation beads, such as CYTODEX™. According to the invention, this small scale format for cell cultivation may be used for any testing involving cells, for example testing of optimal growth conditions for a specific type of cell, such as stem cells. Another use is cell expansion.

摘要翻译： 本发明涉及一种细胞培养方法，更准确地说是小规模细胞培养。 在本发明中，使用了一种筛选工具，其包括连接到固体支持物例如微量滴定板上的颗粒物质或微载体，例如珠，用于在所述微载体上培养细胞。 微载体优选是培养珠，例如CYTODEX TM。 根据本发明，用于细胞培养的这种小规模格式可用于涉及细胞的任何测试，例如测试特定类型细胞如干细胞的最佳生长条件。 另一个用途是细胞扩增。