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    Plasmid purification
    5.
    发明申请
    Plasmid purification 有权
    质粒纯化

    公开(公告)号：US20070161000A1

    公开(公告)日：2007-07-12

    申请号：US10589740

    申请日：2005-02-21

    申请人： James Van Alstine ,  Jan Bergstrom ,  Dag Lindstrom ,  Joachim Stadler

    发明人： James Van Alstine ,  Jan Bergstrom ,  Dag Lindstrom ,  Joachim Stadler

    IPC分类号： C12Q1/68

    CPC分类号： C12N15/1006 ,  B01D15/327 ,  B01D15/34 ,  B01D15/363 ,  B01J41/20

    摘要： The present invention relates to a method of isolating at least one plasmid from other components of a liquid, which method comprises the steps of providing a separation matrix comprised of one or more porous carriers, which carrier(s) present anion exchange groups on external surfaces as well as pore surfaces and a pore size distribution that does not allow access of plasmids to pore surfaces; contacting said matrix with the liquid to allow adsorption of the plasmids to ligands present on the separation matrix; contacting an eluent with the separation matrix to release the plasmids and recovering plasmids from a fraction of said eluent. Thus, the present method allows the plasmids to adsorb to the external surfaces of the matrix, while other components such as RNA is adsorbed onto the pore surfaces. In one embodiment, the matrix presents a DNA exclusion limit of at least about 270 base pairs; such as at least about 1,000 base pairs.

    摘要翻译： 本发明涉及从液体的其它组分分离至少一种质粒的方法，该方法包括以下步骤：提供由一种或多种多孔载体组成的分离基质，该载体在外表面上存在阴离子交换基团 以及孔表面和不允许质粒进入孔表面的孔径分布; 使所述基质与液体接触以允许质粒吸附到存在于分离基质上的配体; 将洗脱液与分离基质接触以释放质粒并从一部分所述洗脱液中回收质粒。 因此，本方法允许质粒吸附到基质的外表面，而诸如RNA的其它组分被吸附到孔表面上。 在一个实施方案中，所述基质呈现至少约270个碱基对的DNA排除极限; 例如至少约1,000个碱基对。

    Magnetic Beads
    7.
    发明申请
    Magnetic Beads 有权
    磁珠

    公开(公告)号：US20080152939A1

    公开(公告)日：2008-06-26

    申请号：US11911540

    申请日：2006-04-13

    申请人： Mathias Alterman ,  Andreas Axen ,  Gunnar Glad ,  Dag Lindstrom ,  Ulrika Meyer ,  Nils Norrman ,  Tobias E. Soderman

    发明人： Mathias Alterman ,  Andreas Axen ,  Gunnar Glad ,  Dag Lindstrom ,  Ulrika Meyer ,  Nils Norrman ,  Tobias E. Soderman

    IPC分类号： H01F1/06 ,  B22F1/00 ,  B22F1/02

    CPC分类号： G01N33/54326 ,  H01F1/0054 ,  H01F1/36 ,  Y10T428/12014 ,  Y10T428/12028 ,  Y10T428/2998

    摘要： The present invention relates to magnetic beads suitable for, for example, isolation of proteins, cells, and viruses and also for diagnostic applications and cell cultivation. The magnetic beads are composite beads with an inner core of metal particles, which are coated with an inert synthetic polymer and these are then enclosed in a hydrophilic porous polymer, preferably agarose. This provides porous biocompatible beads without metal leakage. The beads may be used for cell cultivation or for chromatography. When the beads are used for chromatography the agarose layer is preferably provided with ligands having affinity for selected biomolecules.

    摘要翻译： 本发明涉及适合于例如分离蛋白质，细胞和病毒以及用于诊断应用和细胞培养的磁珠。 磁珠是具有金属颗粒内核的复合珠粒，其中涂有惰性合成聚合物，然后将其包封在亲水性多孔聚合物，优选琼脂糖中。 这提供了多孔的生物相容珠，而没有金属泄漏。 珠粒可用于细胞培养或色谱法。 当珠子用于层析时，琼脂糖层优选具有对选定的生物分子具有亲和性的配体。

    Manufacture of improved support matrices
    8.
    发明授权
    Manufacture of improved support matrices 失效
    改进的支撑矩阵的制造

    公开(公告)号：US07208532B2

    公开(公告)日：2007-04-24

    申请号：US10168709

    申请日：2000-12-11

    申请人： Eva Berggren ,  Dag Lindstrom

    发明人： Eva Berggren ,  Dag Lindstrom

    IPC分类号： C08F2/46 ,  C08F251/00 ,  C08J3/28 ,  B01J20/04 ,  B01J20/24

    CPC分类号： B01J20/285 ,  B01J20/26 ,  B01J20/261 ,  B01J20/262 ,  B01J20/267 ,  B01J20/28011 ,  B01J20/28016 ,  B01J20/28042 ,  B01J2219/00497 ,  B01J2220/52 ,  B01J2220/82 ,  C07B2200/11 ,  C07K1/042 ,  C07K1/16 ,  C08J3/28 ,  C12N5/0068 ,  C12N2533/76 ,  C12N2533/78

    摘要： A method for the production of a cross-linked support matrix that in form of a bed will permit liquid flow velocities above 5 cm/h, preferably above 50 cm/h, to pass through the bed. The method is characterized in that it comprises the steps: (a) providing a starting support matrix that has pendent unsaturated groups, and (b) subjecting said starting support matrix to electron beam or gamma-ray irradiation. The use of a cross-linked support matrix produced by a method comprising the steps of: (a) providing a starting support matrix that has pendent unsaturated groups, and (b) subjecting said starting support matrix to electron beam or gamma-ray irradiation as a support matrix in liquid chromatography, cell culturing, step-wise solid phase synthesis of organic compounds, running catalytic reactions by the use of a solid phase bound catalyst.

    摘要翻译： 用于生产以床形式的交联载体基质的方法将允许高于5cm / h，优选地高于50cm / h的液体流速通过床。 该方法的特征在于其包括以下步骤：（a）提供具有侧链不饱和基团的起始载体基质，和（b）使所述起始载体基质经受电子束或γ射线照射。 使用通过包括以下步骤的方法产生的交联载体基质：（a）提供具有侧链不饱和基团的起始载体基质，和（b）使所述起始载体基质进行电子束或γ射线照射， 液相色谱法中的支持基质，细胞培养，有机化合物的逐步固相合成，通过使用固相结合的催化剂进行催化反应。