Manufacture of a chromatography matrix
    2.
    发明授权
    Manufacture of a chromatography matrix 失效
    色谱柱的制造

    公开(公告)号:US07608187B2

    公开(公告)日:2009-10-27

    申请号:US12093229

    申请日:2006-11-16

    IPC分类号: B01D15/08

    摘要: The present invention relates to method of manufacturing a sulphonate-functionalized (S-functionalized) cation exchanger, which method comprises reacting olefinic groups with bisulphite in the presence of at least one amine oxide, such as N-methyl morpholine oxide (NMO). The olefinic groups of the carrier may be allyl groups; which may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

    摘要翻译: 本发明涉及制备磺酸酯官能化(S-官能化)阳离子交换剂的方法,该方法包括在至少一种氧化胺如N-甲基吗啉氧化物(NMO)存在下使烯基与亚硫酸氢盐反应。 载体的烯基可以是烯丙基; 其可以在增量剂如多羟基官能聚合物上提供。 在一个实施方案中,载体由具有改进的流动压力性质的琼脂糖制成。

    Manufacture of chromatography matrices
    3.
    发明授权
    Manufacture of chromatography matrices 有权
    色谱基质的制造

    公开(公告)号:US08858800B2

    公开(公告)日:2014-10-14

    申请号:US11996357

    申请日:2006-08-30

    摘要: The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

    摘要翻译: 本发明涉及一种制备色谱基质的方法,包括提供包含可用羟基的多糖载体; 并使所述羟基与乙烯基磺酸酯反应以提供磺酸盐官能化的(S-官能化的)阳离子交换剂。 载体的羟基可以是琼脂糖聚合物的羟基; 或者它们可以提供在增量剂上,例如多羟基官能聚合物。 在一个实施方案中,载体由具有改进的流动压力性质的琼脂糖制成。

    Manufacture of Chromatography Matrices
    4.
    发明申请
    Manufacture of Chromatography Matrices 有权
    色谱矩阵的制造

    公开(公告)号:US20080237124A1

    公开(公告)日:2008-10-02

    申请号:US11996357

    申请日:2006-08-30

    IPC分类号: B01D15/08 B01J39/26

    摘要: The present invention relates to a method of manufacturing a chromatography matrix comprising providing a polysaccharide carrier comprising available hydroxyl groups; and reacting said hydroxyl groups with vinyl sulphonate to provide a sulphonate-functionalized (S-functionalized) cation exchanger. The hydroxyl groups of the carrier may be hydroxyls of the agarose polymer; or alternatively they may be provided on extenders such as polyhydroxyfunctional polymers. In one embodiment, the carrier is made of agarose with improved flow pressure properties.

    摘要翻译: 本发明涉及一种制备色谱基质的方法,包括提供包含可用羟基的多糖载体; 并使所述羟基与乙烯基磺酸酯反应以提供磺酸盐官能化的(S-官能化的)阳离子交换剂。 载体的羟基可以是琼脂糖聚合物的羟基; 或者它们可以提供在增量剂上,例如多羟基官能聚合物。 在一个实施方案中,载体由具有改进的流动压力性质的琼脂糖制成。

    SEPARATION MATRIX
    6.
    发明申请
    SEPARATION MATRIX 审中-公开
    分离矩阵

    公开(公告)号:US20100320149A1

    公开(公告)日:2010-12-23

    申请号:US12667873

    申请日:2008-07-22

    IPC分类号: B01D15/38 B01D15/36

    摘要: The present invention relates to a separation matrix, which comprises a support; extenders coupled to an outer part of said support; and ligands coupled to said extenders, wherein the part of the support to which the extenders are coupled constitutes less than 50% of the volume of the separation matrix. The invention also embraces a method of preparing such a separation matrix, as well as a process wherein the separation media is used.

    摘要翻译: 本发明涉及一种分离基质,其包括载体; 联接到所述支撑件的外部的延伸器; 和与所述增量剂偶联的配体,其中与所述增量剂偶联的载体的部分构成小于分离基质体积的50%。 本发明还包括制备这种分离基质的方法,以及其中使用分离介质的方法。

    Magnetic Beads
    7.
    发明申请
    Magnetic Beads 有权
    磁珠

    公开(公告)号:US20080152939A1

    公开(公告)日:2008-06-26

    申请号:US11911540

    申请日:2006-04-13

    IPC分类号: H01F1/06 B22F1/00 B22F1/02

    摘要: The present invention relates to magnetic beads suitable for, for example, isolation of proteins, cells, and viruses and also for diagnostic applications and cell cultivation. The magnetic beads are composite beads with an inner core of metal particles, which are coated with an inert synthetic polymer and these are then enclosed in a hydrophilic porous polymer, preferably agarose. This provides porous biocompatible beads without metal leakage. The beads may be used for cell cultivation or for chromatography. When the beads are used for chromatography the agarose layer is preferably provided with ligands having affinity for selected biomolecules.

    摘要翻译: 本发明涉及适合于例如分离蛋白质,细胞和病毒以及用于诊断应用和细胞培养的磁珠。 磁珠是具有金属颗粒内核的复合珠粒,其中涂有惰性合成聚合物,然后将其包封在亲水性多孔聚合物,优选琼脂糖中。 这提供了多孔的生物相容珠,而没有金属泄漏。 珠粒可用于细胞培养或色谱法。 当珠子用于层析时,琼脂糖层优选具有对选定的生物分子具有亲和性的配体。