公开(公告)号：US10002316B2

公开(公告)日：2018-06-19

申请号：US15667125

申请日：2017-08-02

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Christina Fan ,  Glenn Fu ,  Geoffrey Facer

IPC: G06K19/06 ,  C12Q1/68

CPC classification number: G06K19/06103 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q2525/179 ,  C12Q2543/10 ,  C12Q2563/155

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.

公开(公告)号：US20170342405A1

公开(公告)日：2017-11-30

申请号：US15596364

申请日：2017-05-16

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Eleen Shum

IPC: C12N15/10 ,  C12Q1/68

CPC classification number: C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: The present disclosure relates to compositions, methods and kits for labeling an internal sequence of a target nucleic acid molecule with molecular barcodes. In some embodiments, the methods comprise intramolecular circulation of a labeled target nucleic acid molecule. Further provided methods for generating sequencing libraries comprising overlapping fragments covering the full length of a target nucleic acid molecule, sequencing the libraries using the methods disclosed herein, and methods of analyzing sequencing results therefrom.

公开(公告)号：US09567645B2

公开(公告)日：2017-02-14

申请号：US14472363

申请日：2014-08-28

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

Abstract translation: 本公开提供了用于单个细胞的多重核酸分析的方法，组合物和试剂盒。 方法，组合物和系统可用于大规模并行单细胞测序。 方法，组成和系统可用于同时分析数千个细胞。 数千个细胞可以包含混合的细胞群体（例如，不同类型或亚型的细胞，不同大小的细胞）。

公开(公告)号：US20160265069A1

公开(公告)日：2016-09-15

申请号：US15167807

申请日：2016-05-27

Applicant: Cellular Research Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20160010151A1

公开(公告)日：2016-01-14

申请号：US14800526

申请日：2015-07-15

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US11332776B2

公开(公告)日：2022-05-17

申请号：US16781814

申请日：2020-02-04

Applicant: Cellular Research, Inc.

Inventor： Craig Betts ,  Glenn Fu

IPC: C12P19/34 ,  C12Q1/6806 ,  C12N15/10

Abstract: The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.

公开(公告)号：US10927419B2

公开(公告)日：2021-02-23

申请号：US15987851

申请日：2018-05-23

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20200216877A1

公开(公告)日：2020-07-09

申请号：US16781814

申请日：2020-02-04

Applicant: Cellular Research, Inc.

Inventor： Craig Betts ,  Glenn Fu

IPC: C12Q1/6806 ,  C12N15/10

Abstract: The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.

公开(公告)号：US20180291470A1

公开(公告)日：2018-10-11

申请号：US16012584

申请日：2018-06-19

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US09598736B2

公开(公告)日：2017-03-21

申请号：US15167807

申请日：2016-05-27

Applicant: Cellular Research Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).