公开(公告)号：US11467157B2

公开(公告)日：2022-10-11

申请号：US16789358

申请日：2020-02-12

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Olaf Zoellner

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68 ,  G16B5/10 ,  G16H20/13 ,  G16H10/20 ,  G16B25/10

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US10131958B1

公开(公告)日：2018-11-20

申请号：US16038887

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180327867A1

公开(公告)日：2018-11-15

申请号：US16038979

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180258500A1

公开(公告)日：2018-09-13

申请号：US15987851

申请日：2018-05-23

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180127744A1

公开(公告)日：2018-05-10

申请号：US15806174

申请日：2017-11-07

Applicant: Cellular Research, Inc.

Inventor： Jing Hu ,  Christina Fan ,  David Rosenfeld ,  Jue Fan ,  Elisabeth Marie Walczak

IPC: C12N15/10 ,  G06F19/26

CPC classification number: C12N15/1065 ,  C12Q1/6869 ,  C12Q2525/161 ,  C12Q2535/122 ,  G16B45/00

Abstract: Disclosed herein are methods and systems for classifying cell labels, for example identifying a signal cell label. In some embodiments, the method comprises: obtaining sequencing data of barcoded targets created using targets in cells barcoded using barcodes, wherein a barcode comprises a cell label and a molecular label. After ranking the cell labels, a minimum of a second derivative plot of a cumulative sum plot can be determined. Using the methods, a cell label can be classified as a signal cell label or a noise cell label based on the number of molecular labels with distinct sequences associated with the cell label and a cell label threshold.

公开(公告)号：US20180088112A1

公开(公告)日：2018-03-29

申请号：US15715028

申请日：2017-09-25

Applicant: Cellular Research, Inc. ,  Becton, Dickinson and Company

Inventor： Christina Fan ,  Christina Chang ,  Eleen Shum ,  Olaf Zoellner ,  Nidhanjali Bansal ,  James Ghadiali ,  Gretchen Yinbon Lam ,  Eric Jensen ,  Jurg Rohrer ,  Brent Gaylord ,  Jody Martin ,  Jason G. Vidal

IPC: G01N33/566 ,  C12Q1/68 ,  G01N33/543 ,  C12N15/10

CPC classification number: G01N33/566 ,  B01J2219/00646 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07K2319/01 ,  C12N15/1006 ,  C12N15/1096 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  G01N33/505 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/6842 ,  G01N33/6857 ,  G06F19/00 ,  G16B25/10 ,  G16B99/00 ,  G16H10/20 ,  G16H20/13 ,  C12Q2525/161 ,  C12Q2525/173 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US20180002764A1

公开(公告)日：2018-01-04

申请号：US15459977

申请日：2017-03-15

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn K. Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US09727810B2

公开(公告)日：2017-08-08

申请号：US15055445

申请日：2016-02-26

Applicant: Cellular Research, Inc.

Inventor： Stephen P. A. Fodor ,  Christina Fan ,  Glenn Fu ,  Geoffrey Facer

IPC: G06K19/06 ,  C12Q1/68

CPC classification number: G06K19/06103 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q2525/179 ,  C12Q2543/10 ,  C12Q2563/155

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.

公开(公告)号：US20240376465A1

公开(公告)日：2024-11-14

申请号：US18780091

申请日：2024-07-22

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Christina Fan ,  Elisabeth Marie Walczak

IPC: C12N15/10

Abstract: Disclosed herein include systems, methods, compositions, and kits for the labeling of nucleic acids targets. In some embodiments, the methods comprise the use of target-specific primers and target-non-specific primers during library preparation (e.g., hybrid library preparation). In some embodiments, the methods increase the abundance of select low abundance targets during cDNA library preparation and/or sequencing library preparation.

公开(公告)号：US11618929B2

公开(公告)日：2023-04-04

申请号：US16012584

申请日：2018-06-19

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).