公开(公告)号：US20240263165A1

公开(公告)日：2024-08-08

申请号：US18627190

申请日：2024-04-04

Applicant: Cellular Research, Inc.

Inventor： Philipp Spuhler ,  Sixing Li ,  Dennis E. Prosen

IPC: C12N15/10 ,  B01L3/00

CPC classification number: C12N15/1003 ,  B01L3/5027

Abstract: Disclosed herein include systems, apparatuses, devices, and methods for introducing one or more components into a fluid. A first fluid and a second fluid can be co-injected into a fluidic channel of a flow cell. In some embodiments, the first fluid and a second fluid are immiscible (e.g. an aqueous buffer and a non-aqueous liquid). In some embodiments, the second fluid is less dense than the first fluid.

公开(公告)号：US11976269B2

公开(公告)日：2024-05-07

申请号：US16799464

申请日：2020-02-24

Applicant: Cellular Research, Inc.

Inventor： Philipp Spuhler ,  Sixing Li ,  Dennis Prosen

IPC: C12Q1/68 ,  C12N15/10 ,  B01L3/00

CPC classification number: C12N15/1003 ,  B01L3/5027

Abstract: Disclosed herein include systems, apparatuses, devices, and methods for introducing one or more components into a fluid. A first fluid and a second fluid can be co-injected into a fluidic channel of a flow cell. In some embodiments, the first fluid and a second fluid are immiscible (e.g. an aqueous buffer and a non-aqueous liquid). In some embodiments, the second fluid is less dense than the first fluid.

公开(公告)号：US11932849B2

公开(公告)日：2024-03-19

申请号：US16677012

申请日：2019-11-07

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12N15/10 ,  C40B40/06 ,  C40B50/04 ,  C40B50/06 ,  C40B70/00

CPC classification number: C12N15/1096 ,  C12N15/1006 ,  C40B40/06 ,  C40B50/04 ,  C40B50/06 ,  C40B70/00

Abstract: Disclosed herein include systems, methods, compositions, and kits for whole transcriptome analysis (WTA) with random priming and extension (RPE). The RPE-based WTA method can comprise hybridizing random primers with a plurality of first strand barcoded polynucleotides associated with a solid support and extending the random primers to generate a plurality of extension products. The method can comprise amplifying the plurality of extension products to generate a sequencing library.

公开(公告)号：US11492660B2

公开(公告)日：2022-11-08

申请号：US16707780

申请日：2019-12-09

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12Q1/6837 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6855

Abstract: Disclosed herein include methods and compositions for selectively amplifying and/or extending nucleic acid target molecules in a sample. The methods and compositions can, for example, reduce the amplification and/or extension of undesirable nucleic acid species in the sample, and/or allow selective removal of undesirable nucleic acid species in the sample.

公开(公告)号：US11467157B2

公开(公告)日：2022-10-11

申请号：US16789358

申请日：2020-02-12

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Olaf Zoellner

IPC: G01N33/566 ,  G16B99/00 ,  G01N33/50 ,  G01N33/543 ,  G01N33/68 ,  C12Q1/6804 ,  G16Z99/00 ,  C12N15/10 ,  C12Q1/6806 ,  C12Q1/6823 ,  C12Q1/6869 ,  C12Q1/68 ,  G16B5/10 ,  G16H20/13 ,  G16H10/20 ,  G16B25/10

Abstract: Some embodiments disclosed herein provide a plurality of compositions each comprising a protein binding reagent conjugated with an oligonucleotide. The oligonucleotide comprises a unique identifier for the protein binding reagent it is conjugated with, and the protein binding reagent is capable of specifically binding to a protein target. Further disclosed are methods and kits for quantitative analysis of a plurality of protein targets in a sample and for simultaneous quantitative analysis of protein and nucleic acid targets in a sample. Also disclosed herein are systems and methods for preparing a labeled biomolecule reagent, including a labeled biomolecule agent comprising a protein binding reagent conjugated with an oligonucleotide.

公开(公告)号：US11061043B2

公开(公告)日：2021-07-13

申请号：US16824318

申请日：2020-03-19

Applicant: Cellular Research, Inc.

Inventor： Janice H. Lai ,  Philipp S. Spuhler ,  Geoffrey R. Facer ,  Sixing Li ,  Christopher G. Cesar

IPC: G01N1/00 ,  G01N35/00 ,  C12N15/10 ,  G01N35/10 ,  B01L9/00 ,  B01L3/00

Abstract: Disclosed herein are methods, devices, and systems for loading and retrieval of particles. In some embodiments, a loading station comprise a tray configured to receive a microwell array, a first magnet, a second magnet, and an actuation mechanism configured to cause movement of at least one of the first magnet and the second magnet.

公开(公告)号：US10527171B2

公开(公告)日：2020-01-07

申请号：US15677849

申请日：2017-08-15

Applicant: Cellular Research, Inc.

Inventor： Philipp S. Spuhler ,  Geoffrey R. Facer ,  Sixing Li ,  Christopher G. Cesar

IPC: F16J15/02 ,  B01L3/00 ,  F16J15/14 ,  B01L1/00

Abstract: Disclosed herein are methods, devices, and systems for fluidic handling. In some embodiments, a gasket for providing a fluidic interface with a flowcell includes an inner cavity extending distally from a proximal end of the gasket, the inner cavity being defined by a plurality of inner surfaces sections, an inlet port positioned at a distal end of the inner cavity, an outlet port positioned at a distal end of the gasket, and a cannula extending between the inlet port and the outlet port; wherein at least some of the plurality of inner surface sections are tapered towards the distal end of the gasket to direct a pipette tip received within the gasket towards the inlet port of the gasket.

公开(公告)号：US10202641B2

公开(公告)日：2019-02-12

申请号：US15608780

申请日：2017-05-30

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum

IPC: C12Q1/68 ,  C12Q1/686

Abstract: Disclosed herein are methods and systems for correcting errors in sample amplification, including the errors occurred in determining the number of targets in samples. In some embodiments, the method comprises: stochastically barcoding a plurality of targets in the samples using oligonucleotides comprising stochastic barcodes to generate stochastically barcoded targets; contacting one or more defined barcoded primers with each of the one or more samples; and determining an amplification noise.

公开(公告)号：US10131958B1

公开(公告)日：2018-11-20

申请号：US16038887

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20180327867A1

公开(公告)日：2018-11-15

申请号：US16038979

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).