公开(公告)号：US07785798B2

公开(公告)日：2010-08-31

申请号：US12553225

申请日：2009-09-03

Applicant: Charles R Cantor ,  Chunming Ding

Inventor： Charles R Cantor ,  Chunming Ding

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/166 ,  C12Q2521/331

Abstract: Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.

公开(公告)号：US20090325232A1

公开(公告)日：2009-12-31

申请号：US12553225

申请日：2009-09-03

Applicant: Charles R. Cantor ,  Chunming Ding

Inventor： Charles R. Cantor ,  Chunming Ding

IPC: C12P19/30

CPC classification number: C12Q1/6883 ,  C12Q1/6806 ,  C12Q1/6827 ,  C12Q1/6876 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/166 ,  C12Q2521/331

Abstract: Chromosomal abnormalities are responsible for a significant number of birth defects, including mental retardation. The present invention is related to methods for non-invasive and rapid, prenatal diagnosis of chromosomal abnormalities based on analysis of a maternal blood sample. The invention exploits the differences in DNA between the mother and fetus, for instance differences in their methylation states, as a means to enrich for fetal DNA in maternal plasma sample. The methods described herein can be used to detect chromosomal DNA deletions and duplications. In a preferred embodiment, the methods are used to diagnose chromosomal aneuploidy and related disorders, such as Down's and Turner's Syndrome.

Abstract translation: 染色体异常负责大量的出生缺陷，包括精神发育迟滞。 本发明涉及基于母体血液样品分析的非侵入性和快速，产前诊断染色体异常的方法。 本发明利用母体和胎儿之间的DNA差异，例如甲基化状态的差异，作为富集母体血浆样品中胎儿DNA的手段。 本文描述的方法可用于检测染色体DNA缺失和重复。 在优选的实施方案中，所述方法用于诊断染色体非整倍体和相关疾病，例如唐氏和特纳综合征。

公开(公告)号：US20080081338A1

公开(公告)日：2008-04-03

申请号：US11861809

申请日：2007-09-26

Applicant: Yuk Ming LO ,  Kwan Chee Chan ,  Chunming Ding

Inventor： Yuk Ming LO ,  Kwan Chee Chan ,  Chunming Ding

IPC: C12Q1/68 ,  C07H21/00

CPC classification number: C12Q1/6886 ,  C12Q2600/118 ,  C12Q2600/154

Abstract: The present invention concerns a method for the detection or monitoring of cancer using a biological sample selected from blood, plasma, serum, saliva, urine from an individual, said method comprising: (a) obtaining DNA from the said biological sample; (b) digesting the DNA sample with one or more methylation-sensitive restriction enzymes; (c) quantifying or detecting a DNA sequence of interest after step (b), wherein the target sequence of interest contains at least two methylation-sensitive restriction enzyme recognition sites; and (d) comparing the level of the DNA sequence from the individual to a normal standard, to detect, prognosticate or monitor cancer.

Abstract translation: 本发明涉及使用选自血液，血浆，血清，唾液，个体尿液的生物样品检测或监测癌症的方法，所述方法包括：（a）从所述生物样品中获得DNA; （b）用一种或多种甲基化敏感性限制酶消化DNA样品; （c）在步骤（b）之后量化或检测目标DNA序列，其中所述靶序列含有至少两个甲基化敏感性限制酶识别位点; 和（d）将来自个体的DNA序列的水平与正常标准进行比较，以检测，预测或监测癌症。

公开(公告)号：US20060019278A1

公开(公告)日：2006-01-26

申请号：US11144951

申请日：2005-06-03

Applicant: Yuk Ming Lo ,  Rossa Chiu ,  Stephen Chim ,  Yu-kwan Tong ,  Chunming Ding

Inventor： Yuk Ming Lo ,  Rossa Chiu ,  Stephen Chim ,  Yu-kwan Tong ,  Chunming Ding

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2521/331

Abstract: The present invention relates to new methods for diagnosing a pregnancy-associated disorder by analyzing fetal DNA present in the mother's blood. More specifically, this invention relies on the discovery that the maspin gene is differentially methylated in fetal DNA and in maternal DNA and provides these new diagnostic methods, which distinguish fetal DNA from maternal DNA and detect prenatal disorders based on abnormalities in fetal DNA level and methylation status.

Abstract translation: 本发明涉及通过分析存在于母亲血液中的胎儿DNA来诊断妊娠相关疾病的新方法。 更具体地说，本发明依赖于maspin基因在胎儿DNA和母体DNA中差异甲基化的发现，并提供了这些新的诊断方法，其将胎儿DNA与母体DNA区分开，并根据胎儿DNA水平和甲基化异常检测产前疾病 状态。

公开(公告)号：US20120156685A1

公开(公告)日：2012-06-21

申请号：US13412984

申请日：2012-03-06

Applicant: Charles R Cantor ,  Chunming Ding

Inventor： Charles R Cantor ,  Chunming Ding

IPC: G01N27/62

CPC classification number: C12Q1/6827 ,  C12Q2600/156 ,  C12Q2525/186 ,  C12Q2535/125 ,  C12Q2565/627 ,  C12Q2545/101

Abstract: The present invention is directed to a method for detecting and quantifying rare mutations in a nucleic acid sample. The nucleic acid molecules under investigation can be either DNA or RNA. The rare mutation can be any type of functional or non-functional nucleic acid change or mutation, such as deletion, insertion, translocation, inversion, one or more base substitution or polymorphism. Therefore, the methods of the present invention are useful in detection of rare mutations in, for example, diagnostic, prognostic and follow-up applications, when the targets are rare known nucleic acid variants mixed in with the wildtype or the more common nucleic acid variant(s).

Abstract translation: 本发明涉及一种用于检测和定量核酸样品中罕见突变的方法。 正在研究的核酸分子可以是DNA或RNA。 罕见突变可以是任何类型的功能或非功能性核酸变化或突变，例如缺失，插入，易位，反转，一个或多个碱基取代或多态性。 因此，当目标是罕见的已知的与野生型或更常见的核酸变体混合的核酸变体时，本发明的方法可用于检测例如诊断，预后和随访应用中的罕见突变 （s）。

公开(公告)号：US08034567B2

公开(公告)日：2011-10-11

申请号：US10655762

申请日：2003-09-05

Applicant: Charles R. Cantor ,  Chunming Ding

Inventor： Charles R. Cantor ,  Chunming Ding

IPC: C12Q1/68 ,  C12P19/34 ,  H01J49/00 ,  H01J49/40

CPC classification number: C12Q1/6851 ,  C12Q2545/107 ,  C12Q2535/125 ,  C12Q2525/186

Abstract: The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a “target nucleic acid sequence.” Use of such standard in combination with a method of “enhancing” the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the “enhanced” standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry.

Abstract translation: 本发明涉及使用与目的基因相比具有一个碱基差异的标准或“靶核酸序列”来测量样品中靶核酸量的方法。使用这种标准物 结合使用例如在突变位点上携带的碱基延伸反应来“提高”标准差和测试核酸样品的方法，允许以相同的效率扩增标准品和靶核酸并促进定量 的靶核酸。 此后，使用定量“增强”标准和靶核酸样品的手段来确定靶核酸的量。 在优选实施方案中，定量方法是质谱法。

公开(公告)号：US20100184075A1

公开(公告)日：2010-07-22

申请号：US12704043

申请日：2010-02-11

Applicant: Charles R. Cantor ,  Chunming Ding

Inventor： Charles R. Cantor ,  Chunming Ding

IPC: C12Q1/68

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/137 ,  C12Q2537/143 ,  C12Q2565/627

Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.

Abstract translation: 本发明提供了高通量单倍型分析的有效方法。 可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定 的单倍体存在于受试者。 核酸标记在染色体上可以彼此有任何距离。 此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。 因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

公开(公告)号：US07700325B2

公开(公告)日：2010-04-20

申请号：US10542043

申请日：2004-01-16

Applicant: Charles R Cantor ,  Chunming Ding

Inventor： Charles R Cantor ,  Chunming Ding

IPC: C12P19/34 ,  C12Q1/68 ,  C07H21/02

CPC classification number: C12Q1/6827 ,  C12Q1/6858 ,  C12Q2600/156 ,  C12Q2527/137 ,  C12Q2537/143 ,  C12Q2565/627

Abstract: The present invention provides an efficient way for high throughput haplotype analysis. Several polymorphic nucleic acid markers, such as SNPs, can be simultaneously and reliably determined through multiplex PCR of single nucleic acid molecules in several parallel single molecule dilutions and the consequent statistical analysis of the results from these parallel single molecule multiplex PCR reactions results in reliable determination of haplotypes present in the subject. The nucleic acid markers can be of any distance to each other on the chromosome. In addition, an approach wherein overlapping DNA markers are analyzed can be used to link smaller haplotypes into larger haplotypes. Consequently, the invention provides a powerful new tool for diagnostic haplotyping and identifying novel haplotypes.

Abstract translation: 本发明提供了高通量单倍型分析的有效方法。 可以通过多个平行的单分子稀释物中的单个核酸分子的多重PCR来同时并可靠地测定几种多态性核酸标记，例如SNP，并且随后对来自这些平行的单分子多重PCR反应的结果的统计分析导致可靠的测定 的单倍体存在于受试者。 核酸标记在染色体上可以彼此有任何距离。 此外，分析重叠的DNA标记物的方法可用于将较小的单倍型连接到较大的单元型中。 因此，本发明为诊断单倍型和鉴定新型单体型提供了强大的新工具。

公开(公告)号：US20090155776A1

公开(公告)日：2009-06-18

申请号：US11784501

申请日：2007-04-06

Applicant: Yuk Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Stephen Siu Chung Chim ,  Chunming Ding ,  Kwan Chee Chan ,  Hing Nam Ivy Wong ,  Ka Chun Ryan Yuen

Inventor： Yuk Ming Dennis Lo ,  Rossa Wai Kwun Chiu ,  Stephen Siu Chung Chim ,  Chunming Ding ,  Kwan Chee Chan ,  Hing Nam Ivy Wong ,  Ka Chun Ryan Yuen

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Q2600/154 ,  C12Q2600/156

Abstract: This application describes the discovery that, in a pregnant woman, certain genes (such as RASSF1A, APC, CASP8, RARB, SCGB3A1, DAB2IP, PTPN6, THY1, TMEFF2, and PYCARD) originated from a fetus are highly methylated, whereas the same genes of maternal origin are unmethylated. This discovery allows the easy detection of one or more of these methylated fetal genes in a biological sample from a pregnant woman, serving as a universal indicator of the presence of fetal DNA in the sample. These fetal methylation markers are particularly useful as positive controls for a non-invasive analytical process during which the quality and quantity of fetal DNA are monitored. These newly identified fetal markers can also be measured directly for diagnosis of certain pregnancy-related conditions.

Abstract translation: 该应用描述了在孕妇中发现来自胎儿的某些基因（如RASSF1A，APC，CASP8，RARB，SCGB3A1，DAB2IP，PTPN6，THY1，TMEFF2和PYCARD）高度甲基化，而相同的基因 产妇来源是未甲基化。 该发现允许从孕妇的生物样品中容易地检测这些甲基化胎儿基因中的一种或多种，​​作为样品中胎儿DNA存在的通用指标。 这些胎儿甲基化标记物作为非侵入性分析过程的阳性对照特别有用，在此过程中监测胎儿DNA的质量和数量。 这些新确定的胎儿标记物也可以直接测量以诊断某些妊娠相关病症。

公开(公告)号：US07388061B2

公开(公告)日：2008-06-17

申请号：US10503515

申请日：2003-01-30

Applicant: Mingzhi Gao ,  Haitao Liu ,  Zhulan Li ,  Jun Wang ,  Juxiu Yang ,  Tianyi Li ,  Xiaodong Wang ,  Changxiu Li ,  Chunming Ding

Inventor： Mingzhi Gao ,  Haitao Liu ,  Zhulan Li ,  Jun Wang ,  Juxiu Yang ,  Tianyi Li ,  Xiaodong Wang ,  Changxiu Li ,  Chunming Ding

IPC: C08F4/42

CPC classification number: C08F10/00 ,  C08F110/06 ,  C08F4/651 ,  C08F4/6494 ,  C08F4/6492 ,  C08F4/6465 ,  C08F2500/18 ,  C08F2500/12 ,  C08F2500/15 ,  C08F2500/04

Abstract: The present invention provides a solid catalyst component for the polymerization of olefins, comprising magnesium, titanium, a halogen and an electron donor, wherein said electron donor comprises at least one selected from the group consisting of ester of polyol of the formula (I): R1CO—O—CR3R4—A—CR5R6—O—CO—R2  (I) wherein, R1 and R2 groups, which may be identical or different, can be substituted or unsubstituted hydrocarbyl having 1 to 20 carbon atoms, R3-R6 groups, which may be identical or different, can be selected from the group consisting of hydrogen, halogen or substituted or unsubstituted hydrocarbyl having 1 to 20 carbon atoms, R1-R6 groups optionally contain one or more hetero-atoms replacing carbon, hydrogen atom or the both, said hetero-atom is selected from the group consisting of nitrogen, oxygen, sulfur, silicon, phosphorus and halogen atom, two or more of R3-R6 groups can be linked to form saturated or unsaturated monocyclic or polycyclic ring A is a single bond or bivalent linking group with chain length between two free radicals being 1-10 atoms, wherein said bivalent linking group is selected from the group consisting of aliphatic, alicyclic and aromatic bivalent radicals, and can carry C1-C20 linear or branched substituents one or more of carbon atom and/or hydrogen atom on above-mentioned bivalent linking group and substituents can be replaced by a hetero-atom selected from the group consisting of nitrogen, oxygen, sulfur, silicon, phosphorus, and halogen atom, and two or more said substituents on the linking group as well as above-mentioned R3-R6 groups can be linked to form saturated or unsaturated monocyclic or polycyclic ring.