公开(公告)号：US20160362682A1

公开(公告)日：2016-12-15

申请号：US15156217

申请日：2016-05-16

Applicant: Gigagen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C12N15/10 ,  C07K16/00

CPC classification number: C12N15/1037 ,  C07K14/7051 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6876 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

Abstract translation: 本文公开了用于从免疫细胞产生重组融合多肽的所有组成成分的方法和组合物及其应用。

公开(公告)号：US20150031555A1

公开(公告)日：2015-01-29

申请号：US14374371

申请日：2013-01-24

Applicant: GIGAGEN, INC.

Inventor： David Scott Johnson ,  Andrea Loehr

IPC: C12Q1/68 ,  G06F19/24

CPC classification number: C12Q1/6851 ,  C12Q1/6881 ,  C12Q2600/156 ,  G16B40/00 ,  C12Q2537/143 ,  C12Q2537/165

Abstract: This invention relates a method to correct for bias inherent to multiplexed sequence amplification. The resulting corrected data is a much more accurate representation of true quantities than unprocessed data.

Abstract translation: 本发明涉及一种校正多路复用序列扩增固有偏差的方法。 所得到的校正数据比未处理的数据更真实地表示真实量。

公开(公告)号：US12241178B2

公开(公告)日：2025-03-04

申请号：US17862101

申请日：2022-07-11

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C40B40/08 ,  C07K14/725 ,  C07K16/00 ,  C12N15/10 ,  C12Q1/6876 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US20230124998A1

公开(公告)日：2023-04-20

申请号：US17872042

申请日：2022-07-25

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Shultz Adler ,  Matthew James Spindler ,  Rena Aviva Mizrahi

IPC: C40B40/08 ,  C12N15/10 ,  C12Q1/6888 ,  C07K16/00 ,  C07K14/725 ,  C07K14/74 ,  C12Q1/6869 ,  C40B50/06 ,  C12Q1/6806

Abstract: Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

公开(公告)号：US11427933B2

公开(公告)日：2022-08-30

申请号：US17392892

申请日：2021-08-03

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Shultz Adler ,  Matthew James Spindler ,  Rena Aviva Mizrahi

IPC: C40B50/06 ,  C40B40/08 ,  C12N15/10 ,  C12Q1/6888 ,  C07K16/00 ,  C07K14/725 ,  C07K14/74 ,  C12Q1/6869 ,  C12Q1/6806

Abstract: Provided herein are methods that enable parallel evaluation of multiple functional nucleic acids in individual cells or subpopulations of cells, in the context of incubation with other types of single cells. The key insight is concurrent measurement of polynucleic acids derived from small populations of at least two different cell types, such that function in one cell type is linked to the clonal identity of another cell. These methods simultaneously process thousands, millions, or more single cells or small populations of cells. The method integrates molecular, algorithmic, and engineering approaches. This invention has broad and useful application in a number of biological and medical fields, including immunology and drug discovery.

公开(公告)号：US20220243197A1

公开(公告)日：2022-08-04

申请号：US17607497

申请日：2020-04-30

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Adam Shultz Adler ,  Rena Aviva Mizrahi ,  Yoong Wearn Lim ,  Michael Asensio ,  Sheila Keating ,  Renee Leong ,  Jackson Leong

IPC: C12N15/10 ,  C07K16/08 ,  C07K16/12 ,  C12N15/79

Abstract: Provided herein are compositions comprising recombinant polyclonal proteins (RPPs) derived from mammalian plasma cells and plasmablasts. Also provided are methods of using the RPPs.

公开(公告)号：US11053543B2

公开(公告)日：2021-07-06

申请号：US16885089

申请日：2020-05-27

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/6874 ,  C12N15/10 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6888 ,  C40B50/06 ,  C07K16/00

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US20200291474A1

公开(公告)日：2020-09-17

申请号：US16885089

申请日：2020-05-27

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/6874 ,  C12N15/10 ,  C07K16/00 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6881 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6888

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US10465243B2

公开(公告)日：2019-11-05

申请号：US16132211

申请日：2018-09-14

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/6874 ,  C12N15/10 ,  C07K16/00 ,  C12Q1/6844 ,  C12Q1/6886 ,  C12Q1/6837 ,  C12Q1/6883 ,  C12Q1/6888 ,  C40B50/06

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US10106789B2

公开(公告)日：2018-10-23

申请号：US15431660

申请日：2017-02-13

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12N15/10 ,  C12Q1/68 ,  C40B50/06 ,  C12Q1/6888 ,  C12Q1/6874

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.