公开(公告)号：US07972816B2

公开(公告)日：2011-07-05

申请号：US10567168

申请日：2004-08-09

申请人： Yasuomi Takagi ,  Kazunari Taira ,  Masumi Taki ,  Yoshio Kato ,  Makoto Miyagishi

发明人： Yasuomi Takagi ,  Kazunari Taira ,  Masumi Taki ,  Yoshio Kato ,  Makoto Miyagishi

IPC分类号： C12P19/34 ,  C12Q1/68 ,  C07H21/04

CPC分类号： C12N15/66 ,  A61K31/711 ,  A61K31/713 ,  C12N15/10 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/53 ,  C12N2330/30

摘要： The present invention provides a simple method for producing a dumbbell-shaped DNA.A method for producing a dumbbell-shaped DNA, wherein each of sense and antisense strands is connected at both the 5′ and 3′ ends of a linear-shaped double stranded DNA by a single stranded DNA of loop structure, comprising the steps of; 1) amplifying a target DNA in a template DNA by PCR using sense and antisense primers, wherein each of the sense and antisense primers contains the following sequence (a) at the 5′ end and also contains the following sequences (b), (c), and (d) in order from the 5′ end to the 3′ end, (a) a part of a sense sequence of a nickase recognition sequence, comprising the sequence of a region between the site where a nick is introduced by the action of a nickase and the 3′ end, (b) a sequence capable of forming a loop structure from a single strand, (c) the entire antisense sequence of the nickase recognition sequence (a), (d) a sequence complementary to all or part of the sequence of the target DNA; 2) treating the amplified DNA product of step 1) with a nickase of (a); 3) heating and then annealing the nickase treated amplified DNA product of step 2); and 4) treating the heated and annealed amplified DNA product of step 3) with DNA ligase, wherein the sense and antisense primers used in step 1) are phosphorylated at the 5′ end, or the amplified DNA product is phosphorylated at the 5′ end after step 1) but before step 4).

摘要翻译： 本发明提供了一种制备哑铃型DNA的简单方法。 一种制备哑铃型DNA的方法，其中有义链和反义链中的每一个通过环结构的单链DNA连接在线形双链DNA的5'和3'末端，包括以下步骤： 1）使用正义和反义引物通过PCR扩增模板DNA中的靶DNA，其中有义和反义引物中的每一个在5'末端包含以下序列（a），并且还包含以下序列（b），（c ）和（d）从5'末端到3'末端的顺序，（a）切口酶识别序列的有义序列的一部分，其包含在切口被引入的位点之间的区域的序列 切口酶和3'端的作用，（b）能够从单链形成环结构的序列，（c）切口酶识别序列（a）的整个反义序列，（d）与所有的互补序列互补的序列 或靶DNA的序列的一部分; 2）用（a）的切口酶处理步骤1）的扩增的DNA产物; 3）加热然后对步骤2）的经切割处理的扩增的DNA产物进行退火; 和4）用DNA连接酶处理步骤3）的加热和退火的扩增DNA产物，其中步骤1）中使用的有义和反义引物在5'末端被磷酸化，或者扩增的DNA产物在5'末端被磷酸化 在步骤1之后），但在步骤4之前）。

公开(公告)号：US07923883B2

公开(公告)日：2011-04-12

申请号：US12150059

申请日：2008-04-23

申请人： Kazuyuki Yamaguchi ,  Kenji Endo ,  Yoshio Kato

发明人： Kazuyuki Yamaguchi ,  Kenji Endo ,  Yoshio Kato

IPC分类号： H02K1/00

CPC分类号： H02K15/067

摘要： The present invention provides a stator coiled in a spiral, capable of mechanically inserting a wound coil in the slot of a stator core. In a stator in which coils are overlapped in a spiral when viewed from the end surface of the stator core after each of one sides of a plurality of coils wound in advance is inserted in the slot of the stator core and each of the other sides of the plurality of coils is inserted in a different slot, by repeating the inserting operation twice or more, the loop of the coils overlapped in a spiral are piled in two layers or more in the radius direction of the stator core and the total coil sectional area inserted in the slots of the stator core is larger than a prescribed value based on the inside radius of the stator core.

摘要翻译： 本发明提供一种螺旋状的定子，其能够将缠绕的线圈机械地插入定子芯的槽中。 在从预定的多个线圈的一侧的每一侧插入定子铁芯的槽中的每一个侧面之后，在从定子铁心的端面观察时，线圈重叠成螺旋状的定子中， 通过重复插入操作两次以上，将多个线圈插入到不同的槽中，以螺旋重叠的线圈的环在定子铁芯的半径方向上堆叠成两层以上，总线圈截面积 基于定子铁心的内半径插入定子铁芯的槽中的规定值大于规定值。

公开(公告)号：US07794881B1

公开(公告)日：2010-09-14

申请号：US10110313

申请日：2000-10-20

申请人： Masahisa Fujimoto ,  Shin Fujitani ,  Masaki Shima ,  Hiromasa Yagi ,  Hisaki Tarui ,  Hiroshi Kurokawa ,  Shigeki Matsuta ,  Yoichi Domoto ,  Yoshio Kato ,  Hiroshi Nakajima ,  Hiroaki Ikeda ,  Kenji Asaoka ,  Ryuji Ohshita

发明人： Masahisa Fujimoto ,  Shin Fujitani ,  Masaki Shima ,  Hiromasa Yagi ,  Hisaki Tarui ,  Hiroshi Kurokawa ,  Shigeki Matsuta ,  Yoichi Domoto ,  Yoshio Kato ,  Hiroshi Nakajima ,  Hiroaki Ikeda ,  Kenji Asaoka ,  Ryuji Ohshita

IPC分类号： H01M4/40 ,  H01M4/64

CPC分类号： H01M4/134 ,  H01M2/0207 ,  H01M4/04 ,  H01M4/131 ,  H01M4/1395 ,  H01M4/366 ,  H01M4/38 ,  H01M4/386 ,  H01M4/387 ,  H01M4/40 ,  H01M4/405 ,  H01M4/525 ,  H01M4/58 ,  H01M4/661 ,  H01M4/667 ,  H01M4/70 ,  H01M6/10 ,  H01M6/40 ,  H01M10/052 ,  H01M10/0587 ,  H01M10/44 ,  H01M2010/4292

摘要： An electrode for a lithium battery having a thin film composed of active material capable of lithium storage and release, e.g., a microcrystalline or amorphous silicon thin film, provided on a current collector, the electrode being characterized in that a constituent of the current collector is diffused into the thin film.

摘要翻译： 一种用于锂电池的电极，其具有由集电体上设置的能够蓄积和释放的活性物质（例如微晶或非晶硅薄膜）构成的薄膜，所述电极的特征在于，所述集电体的成分为 扩散到薄膜中。

公开(公告)号：US20080153763A1

公开(公告)日：2008-06-26

申请号：US10567168

申请日：2004-08-09

申请人： Yasuomi Takagi ,  Kazunari Taira ,  Masumi Taki ,  Yoshio Kato ,  Makoto Miyagishi

发明人： Yasuomi Takagi ,  Kazunari Taira ,  Masumi Taki ,  Yoshio Kato ,  Makoto Miyagishi

IPC分类号： A61K31/711 ,  C12P19/34 ,  C12N15/64 ,  C07H21/04

CPC分类号： C12N15/66 ,  A61K31/711 ,  A61K31/713 ,  C12N15/10 ,  C12N15/111 ,  C12N2310/11 ,  C12N2310/53 ,  C12N2330/30

摘要： The present invention provides a simple method for producing a dumbbell-shaped DNA.A method for producing a dumbbell-shaped DNA, wherein each of sense and antisense strands is connected at both the 5′ and 3′ ends of a linear-shaped double stranded DNA by a single stranded DNA of loop structure, comprising the steps of; 1) amplifying a target DNA in a template DNA by PCR using sense and antisense primers, wherein each of the sense and antisense primers contains the following sequence (a) at the 5′ end and also contains the following sequences (b), (c), and (d) in order from the 5′ end to the 3′ end, (a) a part of a sense sequence of a nickase recognition sequence, comprising the sequence of a region between the site where a nick is introduced by the action of a nickase and the 3′ end, (b) a sequence capable of forming a loop structure from a single strand, (c) the entire antisense sequence of the nickase recognition sequence (a), (d) a sequence complementary to all or part of the sequence of the target DNA; 2) treating the amplified DNA product of step 1) with a nickase of (a); 3) heating and then annealing the nickase treated amplified DNA product of step 2); and 4) treating the heated and annealed amplified DNA product of step 3) with DNA ligase, wherein the sense and antisense primers used in step 1) are phosphorylated at the 5′ end, or the amplified DNA product is phosphorylated at the 5′ end after step 1) but before step 4).

摘要翻译： 本发明提供了一种制备哑铃型DNA的简单方法。 一种制备哑铃型DNA的方法，其中有义链和反义链中的每一个通过环结构的单链DNA连接在线形双链DNA的5'和3'末端，包括以下步骤： 1）使用正义和反义引物通过PCR扩增模板DNA中的靶DNA，其中有义和反义引物中的每一个在5'末端包含以下序列（a），并且还包含以下序列（b），（c ）和（d）从5'末端到3'末端的顺序，（a）切口酶识别序列的有义序列的一部分，其包含在切口被引入的位点之间的区域的序列 切口酶和3'端的作用，（b）能够从单链形成环结构的序列，（c）切口酶识别序列（a）的整个反义序列，（d）与所有的互补序列互补的序列 或靶DNA的序列的一部分; 2）用（a）的切口酶处理步骤1）的扩增的DNA产物; 3）加热然后对步骤2）的经切割处理的扩增的DNA产物进行退火; 和4）用DNA连接酶处理步骤3）的加热和退火的扩增DNA产物，其中步骤1）中使用的有义和反义引物在5'末端被磷酸化，或者扩增的DNA产物在5'末端被磷酸化 在步骤1之后），但在步骤4之前）。

公开(公告)号：US07252260B2

公开(公告)日：2007-08-07

申请号：US11183862

申请日：2005-07-19

申请人： Yoshio Kato

发明人： Yoshio Kato

IPC分类号： B21C47/14

CPC分类号： H01F41/066 ,  H01F41/082 ,  H01F41/098

摘要： Provided are a winding method and a winding apparatus in which it makes possible to expedite the winding cycle with a relatively simple construction.In a winding method in which a plurality of conductor wires are arranged in parallel and wound around a reel to form a coil, which is dropped into a coil receiving jig, the plurality of conductor wires are temporarily wound around a bobbin, and the bobbin is attached to a flyer of a winding apparatus. The flyer is rotated to wind the plurality of conductor wires around the reel while drawing them out of the bobbin.

摘要翻译： 提供一种卷绕方法和卷绕装置，其中可以以相对简单的结构加速卷绕周期。 在卷绕方法中，多条导线平行布置并卷绕在卷轴上以形成卷绕在线圈接收夹具中的线圈，多条导线暂时缠绕在线轴上，线轴为 连接到卷绕装置的传单。 将传单旋转以将多根导线缠绕在卷轴上，同时将它们从线轴中拉出。

公开(公告)号：US06685127B2

公开(公告)日：2004-02-03

申请号：US10064545

申请日：2002-07-25

申请人： Tadashi Takano ,  Susumu Ando ,  Yoshio Kato

发明人： Tadashi Takano ,  Susumu Ando ,  Yoshio Kato

IPC分类号： H02K15085

CPC分类号： H02K15/095 ,  Y10T29/49071

摘要： An automatic machine and method of winding of armatures having maximum coil density because the winding nozzle does not enter into the slots between the pole teeth and the wire is restrained at at least one the ends of the core portion of the armature to facilitate winding.

摘要翻译： 由于绕组喷嘴不进入极齿和线之间的槽中的电枢卷绕具有最大线圈密度的自动机器和方法被限制在衔铁的芯部的至少一个端部以便于卷绕。

公开(公告)号：US06685126B2

公开(公告)日：2004-02-03

申请号：US10064543

申请日：2002-07-25

申请人： Tadashi Takano ,  Susumu Ando ,  Yoshio Kato

发明人： Tadashi Takano ,  Susumu Ando ,  Yoshio Kato

IPC分类号： H02K15085

CPC分类号： H02K15/095 ,  Y10T29/49071

摘要： Armature structures and methods that provide very dense coil windings and afford very rapid winding methods at a relatively low cost. In addition the likelihood of damaging the already wound wires by contact with the winding nozzle is substantially eliminated. This is achieved by moving the winding needle in varying distances and directions away from the pole teeth to induce varying degrees of slack in the wire during the winding and when the needle is not in the slot between the pole teeth.

摘要翻译： 电枢结构和方法提供非常密集的线圈绕组，并以相对低的成本提供非常快速的绕组方法。 此外，基本上消除了通过与绕组喷嘴接触而损坏已经缠绕的线的可能性。 这是通过使绕组针在距离极齿不同的距离和方向上移动来实现的，以在绕组期间以及当针不在极齿之间的槽中时引起线中的松弛度变化。

公开(公告)号：US4620137A

公开(公告)日：1986-10-28

申请号：US515253

申请日：1983-07-19

申请人： Yoshio Numaho ,  Yoshio Kato ,  Hiroshi Okada ,  Tetsuro Ogawa

发明人： Yoshio Numaho ,  Yoshio Kato ,  Hiroshi Okada ,  Tetsuro Ogawa

IPC分类号： G01S7/14 ,  G09G1/14 ,  H01J29/78

CPC分类号： G01S7/14

摘要： A sweep termination generating circuit adopts an octagon boundary for sweep termination which is set by combining a plurality of comparators so as to be circumscribed about the display screen of a circular CRT. When the sweep position is deviated from the center of the display screen for the purpose of displaying a radar image by PPI sweep, the circuit allows the comparators to detect that a scanning signal for scanning an electron beam from the off-center sweep position has reached the boundary for sweep termination and thereby generates a sweep termination signal.

摘要翻译： 扫描终止产生电路采用扫描终止的八边形边界，其通过组合多个比较器来设定，以便围绕圆形CRT的显示屏来限定。 当扫描位置偏离显示屏幕的中心以便通过PPI扫描显示雷达图像时，电路允许比较器检测到用于扫描来自偏心扫描位置的电子束的扫描信号已经到达 扫描终止的边界，从而产生扫描终止信号。

公开(公告)号：US4586876A

公开(公告)日：1986-05-06

申请号：US766670

申请日：1985-08-16

申请人： Yoshio Kato ,  Toshihiro Nagamathu ,  Kazufumi Tsurumi ,  Shozo Nakayama ,  Kimio Kato

发明人： Yoshio Kato ,  Toshihiro Nagamathu ,  Kazufumi Tsurumi ,  Shozo Nakayama ,  Kimio Kato

IPC分类号： F04B39/02 ,  F04B27/08 ,  F04B27/10 ,  F04B1/16 ,  F04B1/18

CPC分类号： F04B27/0821 ,  F04B27/1063 ,  F05C2201/0466 ,  F05C2201/0475

摘要： A fluid compressor comprises a cylinder block, a driving shaft rotatably mounted in the cylinder block by means of thrust bearings and radial bearings, an inclined disk rigidly secured to the driving shaft, pistons slidably disposed in cylinder bores formed in the cylinder block, and shoes for operably connecting the inclined disk to the pistons, which are reciprocally moved in the cylinder bores when the inclined disk is rotated to pressurize gaseous fluid for cooling. In order to accommodate the inclined disk, the cylinder block has a housing, which is provided with recesses or grooves on the opposite axial end surfaces to catch the lubrication oil flowing along the inner wall of the housing. Oil ports or passages are also provided for introducing the oil from the recesses or grooves to the radial bearings. Sliding bearings may be used as the radial bearings. Such a sliding bearing may be provided with an oil guide groove or ball indents on the inner peripheral wall thereof.

摘要翻译： 流体压缩机包括气缸体，通过推力轴承和径向轴承可旋转地安装在气缸体中的驱动轴，刚性地固定到驱动轴的倾斜盘，可滑动地设置在形成在气缸体中的气缸孔中的活塞和鞋 用于将斜盘可操作地连接到活塞，当倾斜盘旋转以加压气态流体进行冷却时，活塞在气缸孔中往复运动。 为了容纳斜盘，气缸体具有壳体，该壳体在相对的轴向端面上设置有凹槽或凹槽，以捕获沿着壳体的内壁流动的润滑油。 还提供油​​口或通道用于将油从凹槽或凹槽引导到径向轴承。 滑动轴承可用作径向轴承。 这样的滑动轴承可以在其内周壁上设置有导油槽或球形凹口。

公开(公告)号：US4154900A

公开(公告)日：1979-05-15

申请号：US796196

申请日：1977-05-12

申请人： Yoshihiro Kaku ,  Kiyotaka Hiraoka ,  Mikio Sugiura ,  Yoshio Kato

发明人： Yoshihiro Kaku ,  Kiyotaka Hiraoka ,  Mikio Sugiura ,  Yoshio Kato

IPC分类号： B22D19/00 ,  B22D19/08 ,  B22F7/04 ,  B22F7/08 ,  B23K20/00 ,  B22F3/00

CPC分类号： B22D19/08 ,  B22D19/0081 ,  B22F7/04 ,  B22F7/08 ,  Y10T428/12069 ,  Y10T428/12104 ,  Y10T428/1216 ,  Y10T428/12444 ,  Y10T428/12451

摘要： A composite material of a ferrous cladding material and an aluminum cast matrix which composite material exhibits excellent performance and a method for producing the same which can be carried out quite easily. The composite material comprises a ferrous cladding material, powder particles bonded to the ferrous cladding material, seizing portions defined by the powder particles, and a cast matrix of aluminum or its alloy held by the seizing portions. The method comprises the steps of spreading powder particles over a ferrous cladding material, bonding the powder particles to the ferrous cladding material by sintering, and casting aluminum or its alloy over the sintered surface. Further, a metallic layer can be formed between the bonded powder particles and the cast aluminum, by plating.

摘要翻译： 一种复合材料的铁质包层材料和铝铸造基体，该复合材料表现出优异的性能及其制造方法，可以很容易地进行。 复合材料包括铁包覆材料，结合到含铁包层材料的粉末颗粒，由粉末颗粒限定的捕获部分，以及由捕获部分保持的铝或其合金的浇注基质。 该方法包括以下步骤：将粉末颗粒撒在铁包层材料上，通过烧结将粉末颗粒与铁包层材料接合，并在烧结表面上浇铸铝或其合金。 此外，可以通过电镀在接合的粉末颗粒和铸铝之间形成金属层。